Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927312,9010GA94.9% 713.2 / 17.3 213D247N (GAT→AAT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base G (4/7);  new base A (106/96);  total (110/103)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 215 total aligned reads displayed.

GGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12754‑13045
                                                                                                                                                   |                                                                                                                                                
  tACTTTCGATATTTCTATTATCGATATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCa                                                                                                                                              <  2:3756521/150‑1 (MQ=255)
  tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCa                                                                                                                                              <  2:5140756/150‑1 (MQ=255)
   aCTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAg                                                                                                                                             >  1:1098217/1‑150 (MQ=255)
    cTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAgg                                                                                                                                            >  2:456014/1‑150 (MQ=255)
      ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGc                                                                                                                                          <  1:1100884/150‑1 (MQ=255)
      ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAAACAGGGc                                                                                                                                          >  2:1178149/1‑150 (MQ=255)
       tCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCa                                                                                                                                         >  2:2302706/1‑150 (MQ=255)
         gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       <  1:4690673/150‑1 (MQ=255)
         gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       <  2:4461621/150‑1 (MQ=255)
         gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       >  1:2037824/1‑150 (MQ=255)
          atatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTg                                                                                                                                      <  2:109179/150‑1 (MQ=255)
           tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGa                                                                                                                                     >  1:5160773/1‑150 (MQ=255)
            atTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGAc                                                                                                                                    <  1:5013228/150‑1 (MQ=255)
            atTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    >  1:2674535/1‑150 (MQ=255)
                cTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTgc                                                                                                                                >  1:1503955/1‑150 (MQ=255)
                  attatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCCACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTgcgc                                                                                                                              <  1:2850033/150‑1 (MQ=255)
                     atCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAAc                                                                                                                           >  2:4960861/1‑150 (MQ=255)
                          aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATcc                                                                                                                      <  2:4124039/150‑1 (MQ=255)
                          aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATcc                                                                                                                      >  1:2692402/1‑150 (MQ=255)
                               acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTgg                                                                                                                 >  1:2609522/1‑150 (MQ=255)
                                 gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                               >  1:2351589/1‑150 (MQ=255)
                                 gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                               >  1:3566082/1‑150 (MQ=255)
                                 gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                               <  2:145343/150‑1 (MQ=255)
                                   aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAAt                                                                                                             >  2:1530671/1‑150 (MQ=255)
                                   aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAAt                                                                                                             <  2:16921/150‑1 (MQ=255)
                                   aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAAt                                                                                                             >  1:407608/1‑150 (MQ=255)
                                     ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc                                                                                                           <  2:3430416/150‑1 (MQ=255)
                                     ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc                                                                                                           >  2:3540209/1‑150 (MQ=255)
                                      tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                          >  1:4887034/1‑150 (MQ=255)
                                      tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                          >  1:148395/1‑150 (MQ=255)
                                         cGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcg                                                                                                       >  1:1652096/1‑150 (MQ=255)
                                         cGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcg                                                                                                       >  1:3424826/1‑150 (MQ=255)
                                          ggCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAGTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                      >  2:2360387/1‑150 (MQ=255)
                                           gCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGcc                                                                                                     >  1:914106/1‑150 (MQ=255)
                                               aaaaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaa                                                                                                 <  2:3489327/150‑1 (MQ=255)
                                                 aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaag                                                                                               >  2:2907031/1‑150 (MQ=255)
                                                     cTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGc                                                                                           >  1:2330762/1‑150 (MQ=255)
                                                      ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCg                                                                                          <  1:2106445/150‑1 (MQ=255)
                                                         gAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCa                                                                                       >  1:3275662/1‑150 (MQ=255)
                                                           aGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGa                                                                                     <  2:761453/150‑1 (MQ=255)
                                                            gTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                    >  1:1362504/1‑150 (MQ=255)
                                                               cTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                 >  2:3557079/1‑150 (MQ=255)
                                                               cTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                 >  2:3113429/1‑150 (MQ=255)
                                                                tGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAg                                                                                >  2:2441572/1‑150 (MQ=255)
                                                                tGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAg                                                                                <  2:5042759/150‑1 (MQ=255)
                                                                tGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAg                                                                                >  2:1460552/1‑150 (MQ=255)
                                                                 ggCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGc                                                                               >  2:5048947/1‑150 (MQ=255)
                                                                   caaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                             >  1:3369833/1‑150 (MQ=255)
                                                                   caaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                             <  1:1141656/150‑1 (MQ=255)
                                                                    aaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaa                                                                            >  1:62029/1‑150 (MQ=255)
                                                                            gTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAAc                                                                    >  2:1539369/1‑150 (MQ=255)
                                                                             tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACt                                                                   >  1:1045730/1‑150 (MQ=255)
                                                                              gATACCCAACTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCGGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGTAAATGCGAAAATCGAAATg                                                                  >  2:3926117/1‑150 (MQ=255)
                                                                                 aCCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                               <  2:2104539/150‑1 (MQ=255)
                                                                                 aCCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                               <  2:4172550/150‑1 (MQ=255)
                                                                                    cACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTcc                                                            <  2:661345/150‑1 (MQ=255)
                                                                                    cACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTcc                                                            <  1:2148891/150‑1 (MQ=255)
                                                                                        tGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                        >  1:2669995/1‑150 (MQ=255)
                                                                                        tGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                        <  1:3827735/150‑1 (MQ=255)
                                                                                          gggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGACAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTcag                                                      >  2:3444373/1‑150 (MQ=255)
                                                                                              gTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGa                                                  >  2:3051536/1‑150 (MQ=255)
                                                                                              gTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGa                                                  >  1:5168067/1‑150 (MQ=255)
                                                                                               tGAAGACTTCGACAGCCGTCTGATCAACTATGTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAc                                                 <  1:31977/150‑1 (MQ=255)
                                                                                                 aaGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                               <  2:4119757/150‑1 (MQ=255)
                                                                                                  aGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGa                                              >  1:2890318/1‑150 (MQ=255)
                                                                                                   gACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGAc                                             <  2:2331061/150‑1 (MQ=255)
                                                                                                       tCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTa                                         >  1:3240565/1‑150 (MQ=255)
                                                                                                       tCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTa                                         <  2:3138822/150‑1 (MQ=255)
                                                                                                         gACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAc                                       <  2:4393699/150‑1 (MQ=255)
                                                                                                          aCAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAcc                                      >  1:3849277/1‑150 (MQ=255)
                                                                                                          aCAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAcc                                      <  1:3113429/150‑1 (MQ=255)
                                                                                                           cAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCt                                     >  2:3704645/1‑150 (MQ=255)
                                                                                                           cAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCt                                     >  1:2255020/1‑150 (MQ=255)
                                                                                                            aGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTg                                    >  2:1038025/1‑150 (MQ=255)
                                                                                                              ccGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGcc                                  <  1:4443736/150‑1 (MQ=255)
                                                                                                               cGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCa                                 <  1:3329482/150‑1 (MQ=255)
                                                                                                                gTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCAt                                <  1:188174/150‑1 (MQ=255)
                                                                                                                  cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATAc                              >  2:552395/1‑150 (MQ=255)
                                                                                                                  cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATAc                              >  2:1443203/1‑150 (MQ=255)
                                                                                                                  cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATAc                              >  2:4880880/1‑150 (MQ=255)
                                                                                                                     aTCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATc                           >  1:1721124/1‑150 (MQ=255)
                                                                                                                       cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCTGCAGACCGACGTTAACCTGCCATACATCAc                         <  1:4714303/150‑1 (MQ=255)
                                                                                                                       cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         <  2:2609522/150‑1 (MQ=255)
                                                                                                                        aaCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACt                        >  1:2501071/1‑150 (MQ=255)
                                                                                                                            aTCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                    >  2:329387/1‑150 (MQ=255)
                                                                                                                            aTCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                    <  1:4995864/150‑1 (MQ=255)
                                                                                                                              cTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAc                  <  2:1910266/150‑1 (MQ=255)
                                                                                                                               tGGTTGAAGAATTCATGAAAAATCAGGGCATTGACCTGCGCAACGATCCTCTGGCAATGCTGCGCCTGAATGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCTGCAGTCCGTCGTTAACCTGCCATTCATCACTGCAGAcg                 <  1:973588/150‑1 (MQ=255)
                                                                                                                               tGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcg                 <  1:3211177/150‑1 (MQ=255)
                                                                                                                               tGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcg                 <  1:2810194/150‑1 (MQ=255)
                                                                                                                                 gTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgcg               <  2:1140154/150‑1 (MQ=255)
                                                                                                                                   tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAc             <  1:5101500/150‑1 (MQ=255)
                                                                                                                                   tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAc             >  1:2007574/1‑150 (MQ=255)
                                                                                                                                    gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAACGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAcc            <  1:2465915/150‑1 (MQ=255)
                                                                                                                                    gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAcc            <  1:4616996/150‑1 (MQ=255)
                                                                                                                                     aagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCg           <  2:4713090/150‑1 (MQ=255)
                                                                                                                                        aaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTc        >  2:3362681/1‑150 (MQ=255)
                                                                                                                                          ttCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCg      <  2:709851/150‑1 (MQ=255)
                                                                                                                                           tCATGAAAAATCCGGGCATTGACGTGCGACACGATCCGCTGGCAATGCAGCGCCTTAATTAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTCCAGACGCGACCGGTCCGa     <  1:1789531/150‑1 (MQ=255)
                                                                                                                                              agaaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAac  >  2:3963831/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12754‑13045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑