Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,482,981 C→A 10.0% E229* (GAA→TAA)  yfdV ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,482,9810CA10.0% 666.6 / 43.0 197E229* (GAA→TAA) yfdVputative transporter
Reads supporting (aligned to +/- strand):  ref base C (105/72);  new base A (10/10);  total (115/82)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

GGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACAG  >  CP009273/2482832‑2483130
                                                                                                                                                     |                                                                                                                                                     
ggCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTc                                                                                                                                                       <  2:249999/150‑1 (MQ=255)
  cAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTctg                                                                                                                                                     <  2:3094882/150‑3 (MQ=255)
  cAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCgg                                                                                                                                                     <  2:4997058/150‑1 (MQ=255)
   aataatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGc                                                                                                                                                    >  1:2323292/1‑150 (MQ=255)
     taatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCAc                                                                                                                                                  >  1:2232516/1‑150 (MQ=255)
      aatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGCAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACt                                                                                                                                                 <  1:1198777/150‑1 (MQ=255)
      aatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACt                                                                                                                                                 >  1:4886303/1‑150 (MQ=255)
      aatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACt                                                                                                                                                 >  1:3020074/1‑150 (MQ=255)
      aatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACt                                                                                                                                                 >  2:4176903/1‑150 (MQ=255)
      aatGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACt                                                                                                                                                 <  2:4701319/150‑1 (MQ=255)
       atGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTg                                                                                                                                                >  2:4650388/1‑150 (MQ=255)
        tGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGa                                                                                                                                               >  1:4242595/1‑150 (MQ=255)
         gATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACTGaa                                                                                                                                              >  1:2748107/1‑150 (MQ=255)
            tCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTc                                                                                                                                           >  1:4337270/1‑150 (MQ=255)
               ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGaa                                                                                                                                        <  1:4159129/150‑1 (MQ=255)
                  gaATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAAttt                                                                                                                                     >  1:1158849/1‑150 (MQ=255)
                    aTGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAt                                                                                                                                   <  1:1665857/150‑1 (MQ=255)
                    aTGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAt                                                                                                                                   >  1:5078363/1‑150 (MQ=255)
                        cggcggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGc                                                                                                                               >  1:885888/1‑150 (MQ=255)
                        cggcggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGc                                                                                                                               >  1:3450980/1‑150 (MQ=255)
                           cggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGc                                                                                                                            >  1:4959806/1‑150 (MQ=255)
                            ggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGcc                                                                                                                           >  2:869691/1‑150 (MQ=255)
                            ggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACTGAACTCGAATTTATGTGCAGcc                                                                                                                           >  1:762744/1‑150 (MQ=255)
                               aaTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCaga                                                                                                                        <  2:3776581/150‑1 (MQ=255)
                               aaTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCaga                                                                                                                        <  1:1598166/150‑1 (MQ=255)
                                aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCagag                                                                                                                       >  1:3115033/1‑150 (MQ=255)
                                aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCagag                                                                                                                       <  2:4291467/150‑1 (MQ=255)
                                aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCagag                                                                                                                       <  2:4449388/150‑1 (MQ=255)
                                  gcgcCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTc                                                                                                                     >  1:1882551/1‑150 (MQ=255)
                                         ccAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGAAATTTAGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCg                                                                                                              <  1:5185369/150‑1 (MQ=255)
                                              aCCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAt                                                                                                                                   <  2:2098960/124‑1 (MQ=255)
                                                catcatCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGaa                                                                                                       >  2:929274/1‑150 (MQ=255)
                                                   catcTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATAc                                                                                                    >  1:4639785/1‑150 (MQ=255)
                                                   catcTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCAAGAGTCAACCCCGCAGCGAATAc                                                                                                    >  1:2053485/1‑150 (MQ=255)
                                                    atcTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATAcc                                                                                                   >  1:1121086/1‑150 (MQ=255)
                                                       tGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCt                                                                                                >  2:2594240/1‑150 (MQ=255)
                                                         cAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTAc                                                                                              <  1:1253465/150‑1 (MQ=255)
                                                          aGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTAcc                                                                                             >  1:2194346/1‑150 (MQ=255)
                                                          aGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTAcc                                                                                             >  2:1930379/1‑150 (MQ=255)
                                                             tGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCt                                                                                          <  1:4115371/150‑1 (MQ=255)
                                                             tGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCt                                                                                          <  2:3872036/150‑1 (MQ=255)
                                                               ttCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGa                                                                                        <  1:4555896/150‑1 (MQ=255)
                                                               ttCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGa                                                                                        <  1:3530809/150‑1 (MQ=255)
                                                                 cGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGt                                                                                      >  2:709700/1‑150 (MQ=255)
                                                                   cTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTa                                                                                    >  1:4579910/1‑150 (MQ=255)
                                                                   cTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTa                                                                                    <  1:515921/150‑1 (MQ=255)
                                                                    tGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGACTACCGCTACCCCTGAGTTAg                                                                                   >  2:4355080/1‑150 (MQ=255)
                                                                     gTTCAAATGACATGCCATACCATCGAGAAGCAGTGCCTGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGc                                                                                  <  2:5167338/150‑1 (MQ=255)
                                                                     gTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGc                                                                                  <  2:140398/150‑1 (MQ=255)
                                                                              aCATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGACAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAAt                                                                         <  1:2098730/150‑1 (MQ=255)
                                                                                 tGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAg                                                                      <  1:4259563/150‑1 (MQ=255)
                                                                                 tGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAg                                                                      <  1:2939020/150‑1 (MQ=255)
                                                                                   ccATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGAt                                                                    >  2:3327037/1‑150 (MQ=255)
                                                                                    cATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGAtt                                                                   >  2:1738635/1‑150 (MQ=255)
                                                                                     aTACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTa                                                                  <  2:3583351/150‑1 (MQ=255)
                                                                                     aTACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTa                                                                  <  2:3655323/150‑1 (MQ=255)
                                                                                        ccAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAg                                                               >  1:2421964/1‑150 (MQ=255)
                                                                                        ccAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAg                                                               <  2:2320122/150‑1 (MQ=255)
                                                                                        ccAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAg                                                               >  2:3877570/1‑150 (MQ=255)
                                                                                        ccAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAg                                                               >  1:2119770/1‑150 (MQ=255)
                                                                                          aaCGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGt                                                             >  2:1269806/1‑150 (MQ=255)
                                                                                           aCGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGtt                                                            <  2:4802205/150‑1 (MQ=255)
                                                                                           aCGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGtt                                                            <  2:326666/150‑1 (MQ=255)
                                                                                            cGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTg                                                           <  1:3356057/150‑1 (MQ=255)
                                                                                             gagaAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTgg                                                          <  2:2225426/150‑1 (MQ=255)
                                                                                             gagaAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTgg                                                          >  2:4688859/1‑150 (MQ=255)
                                                                                                  gCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTccc                                                     <  1:1930379/150‑1 (MQ=255)
                                                                                                    aGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCAt                                                   <  2:509323/150‑1 (MQ=255)
                                                                                                      tGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCAtgc                                                 <  2:2711149/150‑1 (MQ=255)
                                                                                                      tGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCAtgc                                                 <  1:4467953/150‑1 (MQ=255)
                                                                                                         cAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCAtgctgc                                              >  2:3880273/1‑150 (MQ=255)
                                                                                                           gaggCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTACGGCACTGAACTCGAATTTATGAGCAGCCAGAGTCAACCCCGCAGCGAATAACGCAACCCCAGAGTTATACATCGCAATCAGATTAAATGTTGTCACCCAAGCTGCCg                                            >  1:4008935/3‑150 (MQ=255)
                                                                                                             ggCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGa                                          >  2:4640261/1‑150 (MQ=255)
                                                                                                              gCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGaa                                         >  1:4778713/1‑150 (MQ=255)
                                                                                                              gCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTAGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGaa                                         >  1:1276987/1‑150 (MQ=255)
                                                                                                                aTCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAAtt                                       <  2:2596216/150‑1 (MQ=255)
                                                                                                                aTCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAAtt                                       >  2:4515238/1‑150 (MQ=255)
                                                                                                                  cAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAAtttt                                     <  1:360478/150‑1 (MQ=255)
                                                                                                                   aGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAAttttt                                    <  1:1459778/150‑1 (MQ=255)
                                                                                                                   aGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAAttttt                                    >  1:1422713/1‑150 (MQ=255)
                                                                                                                     aaTCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTAc                                  <  2:1882551/150‑1 (MQ=255)
                                                                                                                      aTCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTAcc                                 >  1:2638576/1‑150 (MQ=255)
                                                                                                                       tCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTAccc                                <  2:2535494/150‑1 (MQ=255)
                                                                                                                           cTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAGCTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCccaac                            >  1:63480/1‑150 (MQ=255)
                                                                                                                            ttCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCccaacc                           >  2:2783141/1‑150 (MQ=255)
                                                                                                                            ttCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCccaacc                           >  1:3281540/1‑150 (MQ=255)
                                                                                                                               aGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCcaaccaac                        <  2:195379/150‑1 (MQ=255)
                                                                                                                               aGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCcaaccaac                        >  1:5012146/1‑150 (MQ=255)
                                                                                                                                  aaGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACAcc                     <  2:4886303/150‑1 (MQ=255)
                                                                                                                                    ggTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAg                   <  1:2908480/150‑1 (MQ=255)
                                                                                                                                       gtTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGAt                >  2:1669185/1‑150 (MQ=255)
                                                                                                                                        ttATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATc               <  2:3714552/150‑1 (MQ=255)
                                                                                                                                          ataAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCAGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGt             >  1:100003/1‑150 (MQ=255)
                                                                                                                                            aaGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTg           <  2:3115033/150‑1 (MQ=255)
                                                                                                                                              gCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGcc         <  1:929274/150‑1 (MQ=255)
                                                                                                                                               cAATTTCGGCACTGAACTCGAATTTATGTGAAGCCATAGTCAACCCCGCAGCGATTACAGCTACCCCTGTGTTAGCCTTCGCAATCAGATTAAAGTTTGGGTCCCATGCTGCCTGAATTTTTACCCCAACCAACACCAGGATCGTTTCCa        <  2:4008935/150‑1 (MQ=255)
                                                                                                                                                aaTTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAg       >  2:1112431/1‑150 (MQ=255)
                                                                                                                                                aaTTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAg       >  2:1117570/1‑150 (MQ=255)
                                                                                                                                                   ttCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAAc    <  2:1340245/150‑1 (MQ=255)
                                                                                                                                                    tCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACa   <  2:2372415/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACAG  >  CP009273/2482832‑2483130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑