Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 158,287 C→A 90.2% D78Y (GAC→TAC)  ligT ← 2'‑5' RNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273158,2870CA90.2% 800.7 / 74.2 264D78Y (GAC→TAC) ligT2'‑5' RNA ligase
Reads supporting (aligned to +/- strand):  ref base C (11/15);  new base A (109/129);  total (120/144)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 272 total aligned reads displayed.

TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAA  >  CP009273/158140‑158434
                                                                                                                                                   |                                                                                                                                                   
 aaTATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatc                                                                                                                                                  >  1:1577566/1‑150 (MQ=255)
 aaTATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatc                                                                                                                                                  <  2:5180266/150‑1 (MQ=255)
  atatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                                 >  1:1492167/1‑150 (MQ=255)
  atatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtaatca                                                                                                                                                 >  2:4776451/1‑150 (MQ=255)
   tatGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCaa                                                                                                                                                >  1:788718/1‑150 (MQ=255)
     tgtgGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGc                                                                                                                                              >  2:3435116/1‑150 (MQ=255)
       tgGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGt                                                                                                                                            >  2:676041/1‑150 (MQ=255)
         gATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGa                                                                                                                                          >  2:1308061/1‑150 (MQ=255)
            gAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAgtg                                                                                                                                       >  2:1387363/1‑150 (MQ=255)
             aaaCGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAgtgt                                                                                                                                      >  1:4875234/1‑150 (MQ=255)
                cGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaa                                                                                                                                   <  1:1909752/150‑1 (MQ=255)
                 ggACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGaaa                                                                                                                                  <  1:3367451/150‑1 (MQ=255)
                   aCGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                                >  2:258957/1‑150 (MQ=255)
                   aCGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATAGTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAAcc                                                                                                                                <  1:3129508/150‑1 (MQ=255)
                    cGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                               >  1:1401632/1‑150 (MQ=255)
                    cGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                               <  1:3477281/150‑1 (MQ=255)
                    cGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCa                                                                                                                               <  1:1107661/150‑1 (MQ=255)
                     gagtGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                              <  2:1109356/147‑1 (MQ=255)
                     gATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAg                                                                                                                              >  1:4233042/1‑150 (MQ=255)
                        tGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGtt                                                                                                                           <  1:2204244/150‑1 (MQ=255)
                        tGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGtt                                                                                                                           >  2:888141/1‑150 (MQ=255)
                         gCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTg                                                                                                                          <  2:772163/150‑1 (MQ=255)
                         gCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTg                                                                                                                          <  1:573164/150‑1 (MQ=255)
                          cTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGa                                                                                                                         <  2:2244636/150‑1 (MQ=255)
                          cTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGa                                                                                                                         >  2:2805579/1‑150 (MQ=255)
                          cTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGa                                                                                                                         <  1:2704749/150‑1 (MQ=255)
                           tttGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGAc                                                                                                                        >  2:1974875/1‑150 (MQ=255)
                             tGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGa                                                                                                                      >  1:2625495/1‑150 (MQ=255)
                                aaaCAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtc                                                                                                                   <  1:3679863/150‑1 (MQ=255)
                                  ncaacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccg                                                                                                                 >  1:2991399/2‑150 (MQ=255)
                                  acaacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccg                                                                                                                 >  2:3811270/1‑150 (MQ=255)
                                    aacCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtc                                                                                                               >  1:953350/1‑150 (MQ=255)
                                     acCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtcc                                                                                                              >  1:912415/1‑150 (MQ=255)
                                      ccGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAAtccgtccg                                                                                                             >  2:4180934/1‑150 (MQ=255)
                                       cGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCgg                                                                                                            >  1:2249366/1‑150 (MQ=255)
                                          tGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTa                                                                                                         >  1:282983/1‑150 (MQ=255)
                                                 aGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga                                                                                                  <  2:4118838/150‑1 (MQ=255)
                                                   ccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaaa                                                                                                <  1:4405907/150‑1 (MQ=255)
                                                   ccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaaa                                                                                                <  2:2410338/150‑1 (MQ=255)
                                                   ccTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGaaa                                                                                                <  1:2288972/150‑1 (MQ=255)
                                                     tgtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgc                                                                                              <  2:1436233/150‑1 (MQ=255)
                                                      gtgAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAgcg                                                                                             <  2:2624894/150‑1 (MQ=255)
                                                           cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtc                                                                                        >  1:3181692/1‑150 (MQ=255)
                                                           cGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtc                                                                                        <  1:3503180/150‑1 (MQ=255)
                                                            ggAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGTCGAATCAGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                       <  2:3228022/150‑1 (MQ=255)
                                                            ggAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                       <  1:1645935/150‑1 (MQ=255)
                                                            ggAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTtct                                                                                       >  2:1010561/1‑150 (MQ=255)
                                                              aGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTctct                                                                                     >  2:3160141/1‑150 (MQ=255)
                                                                   atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAACCCAGGTTGACGAATCCGTCCGGCTAAAAGAGAACGCGCCTTCTCTTtctc                                                                                >  1:3631825/1‑150 (MQ=255)
                                                                   atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtctc                                                                                <  1:1362335/150‑1 (MQ=255)
                                                                   atTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTtctc                                                                                >  1:3279692/1‑150 (MQ=255)
                                                                       gCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGgcg                                                                            <  2:42226/150‑1 (MQ=255)
                                                                       gCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGgcg                                                                            <  2:717074/150‑1 (MQ=255)
                                                                         cAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCt                                                                          <  1:614447/150‑1 (MQ=255)
                                                                          aGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGACACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTc                                                                         <  1:2744196/150‑1 (MQ=255)
                                                                          aGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTc                                                                         >  2:5025237/1‑150 (MQ=255)
                                                                          aGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTc                                                                         >  2:5001481/1‑150 (MQ=255)
                                                                           gCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCa                                                                        <  2:587533/150‑1 (MQ=255)
                                                                            cTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAc                                                                       >  2:2198710/1‑150 (MQ=255)
                                                                             tGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACt                                                                      >  2:3984283/1‑150 (MQ=255)
                                                                               gATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTc                                                                    <  1:19901/150‑1 (MQ=255)
                                                                                 ttAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGc                                                                  >  2:4511895/1‑150 (MQ=255)
                                                                                   aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCt                                                                <  2:2454948/150‑1 (MQ=255)
                                                                                   aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCt                                                                <  2:2750972/150‑1 (MQ=255)
                                                                                   aaGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCt                                                                >  2:3197344/1‑150 (MQ=255)
                                                                                      ccGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTTAAAGAGCAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTaaa                                                             <  2:3354081/150‑1 (MQ=255)
                                                                                      ccGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTaaa                                                             >  2:86038/1‑150 (MQ=255)
                                                                                          gTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCGATTGTCCGGCGTAATCAAGCGTTAGTGTGAAACCAGGTTGACGAATCCGTCGGGCTAAAAGAGAAAGCGCCTTCTATTTCTCGGCGCTCACTTCGCCTAAAAATg                                                         <  1:4801437/150‑1 (MQ=255)
                                                                                              cggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                     <  1:2074889/150‑1 (MQ=255)
                                                                                              cggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCag                                                     <  2:3333809/150‑1 (MQ=255)
                                                                                               ggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCaga                                                    >  2:2027805/1‑150 (MQ=255)
                                                                                               ggcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCaga                                                    >  1:4746553/1‑150 (MQ=255)
                                                                                                gcTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCagag                                                   <  1:2546639/150‑1 (MQ=255)
                                                                                                  tGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTc                                                 <  1:4054607/150‑1 (MQ=255)
                                                                                                  tGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGAGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCACTTTCTCGGCGCTCACTTCGCCTAAAAATGCCagagac                                                 >  1:3437356/1‑148 (MQ=255)
                                                                                                     cGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGa                                              <  2:368322/150‑1 (MQ=255)
                                                                                                        aTCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCCGAGTCAGATGc                                           >  2:1237779/1‑150 (MQ=255)
                                                                                                        aTCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGc                                           >  2:4885462/1‑150 (MQ=255)
                                                                                                         tCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCa                                          <  1:5076893/150‑1 (MQ=255)
                                                                                                           ccTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCaaa                                        <  2:2253631/150‑1 (MQ=255)
                                                                                                            cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCCAAt                                       >  2:3054296/1‑150 (MQ=255)
                                                                                                            cTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAAt                                       <  1:3915130/150‑1 (MQ=255)
                                                                                                               accacaccacACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                    <  1:1118735/150‑1 (MQ=255)
                                                                                                               accacaccacACGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTAt                                    <  1:1943944/150‑1 (MQ=255)
                                                                                                                 cacaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGTGTCAGATGCAAATTATcg                                  <  2:4016430/150‑1 (MQ=255)
                                                                                                                 cacaccacacGCGAACGCAGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATcg                                  <  1:4118092/150‑1 (MQ=255)
                                                                                                                          cgcgAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCACATTATCGGCGGCGAcc                         >  2:4665866/1‑150 (MQ=255)
                                                                                                                               aCGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCAGTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACg                    <  1:1755722/150‑1 (MQ=255)
                                                                                                                               aCGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACg                    <  1:1755560/150‑1 (MQ=255)
                                                                                                                                   aGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTccc                >  2:1708432/1‑150 (MQ=255)
                                                                                                                                   aGCCATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTccc                >  1:90490/1‑150 (MQ=255)
                                                                                                                                   aGCCATCGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTccc                <  2:3156939/150‑1 (MQ=255)
                                                                                                                                      cATTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGcc             <  1:4507356/150‑1 (MQ=255)
                                                                                                                                       aTTGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCt            >  1:4958568/1‑150 (MQ=255)
                                                                                                                                         tGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGTCGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCa          <  2:4307024/150‑1 (MQ=255)
                                                                                                                                         tGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCa          <  2:308016/150‑1 (MQ=255)
                                                                                                                                         tGTCCGGCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCa          <  2:1852524/150‑1 (MQ=255)
                                                                                                                                          gTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAg         <  2:1787474/150‑1 (MQ=255)
                                                                                                                                               gCGTAATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTggg    <  2:3532588/150‑1 (MQ=255)
                                                                                                                                                 gTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGaa  <  1:4614247/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TAATATGTGGATGAAACGGACGATTGCTTTGAAAACAACCGCTGCGGGCAGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGAAAGCGCCTTCTCTTTCTCGGCGCTCACTTCGCCTAAAAATGCCAGAGTCAGATGCAAATTATCGGCGGCGACCGGACGTCCCGCCTCAGGTGGGAA  >  CP009273/158140‑158434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑