Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,707,492 A→C 8.1% K413T (AAG→ACG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,707,4920AC 8.1% 575.8 / 12.8 207K413T (AAG→ACG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (124/61);  new base C (2/20);  total (126/81)
Fisher's exact test for biased strand distribution p-value = 1.55e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

GCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACT  >  CP009273/2707345‑2707641
                                                                                                                                                   |                                                                                                                                                     
      atatTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCggg                                                                                                                                               >  1:5082991/1‑150 (MQ=255)
      atatTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCggg                                                                                                                                               >  2:675125/1‑150 (MQ=255)
       tatTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGATAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGt                                                                                                                                              <  2:3879809/150‑1 (MQ=255)
        atTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTg                                                                                                                                             >  1:1492427/1‑150 (MQ=255)
            aaGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaaaa                                                                                                                                         <  2:2145081/150‑1 (MQ=255)
            aaGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaaaa                                                                                                                                         >  1:1865651/1‑150 (MQ=255)
            aaGAGCCAATTAAAGCTCGCGTTATTGAGGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaaaa                                                                                                                                         >  1:2356579/1‑150 (MQ=255)
              gagCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaaaaaa                                                                                                                                       >  1:1389257/1‑150 (MQ=255)
               agCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAAc                                                                                                                                      >  2:3884724/1‑150 (MQ=255)
                   aaTTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCa                                                                                                                                  <  2:2454630/150‑1 (MQ=255)
                    aTTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCAt                                                                                                                                 >  1:3202431/1‑150 (MQ=255)
                       aaaGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAATGAGAAGCCGCGGGTGAAAAAACGCCATcgc                                                                                                                              >  2:3553091/1‑150 (MQ=255)
                        aaGCTCGAGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGAAAGCCATCTAATAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATcgcg                                                                                                                             <  2:3640297/150‑1 (MQ=255)
                         aGCTCGCGTTTTTGATGAGTTACGCCCTAAAACGCGTGCGCCACGCTAAAAGCAGACCGGCACGCCATCGAAGAAAGTACTGGATAAACGTGCTGATAAGAAAAAAGCTAAACAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGa                                                                                                                            <  2:1318475/150‑1 (MQ=255)
                          gCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGac                                                                                                                           <  2:2078778/150‑1 (MQ=255)
                            tNGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGacac                                                                                                                         >  1:4663892/1‑150 (MQ=255)
                                gTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCaaa                                                                                                                     >  1:3720892/1‑150 (MQ=255)
                                gTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCaaa                                                                                                                     <  2:4673239/150‑1 (MQ=255)
                                 ttATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCacaa                                                                                                                    >  2:4520858/1‑150 (MQ=255)
                                  tATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCaaaaa                                                                                                                   >  1:4040356/1‑150 (MQ=255)
                                   aTTTATGATTTACGCCCTAAAACGCTTGCGCCAAGCTAAAAGCAGACCGGAAAGCCATCGAATAAATTACTGGCTAAACGTGCTTAGAATAAAAAAGATAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAat                                                                                                                  <  1:4093966/150‑1 (MQ=255)
                                     tgatgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCTAAGAATGTACTGGATAAACGTGCTGATAAGAAAAAAGCTAAAGACAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAatat                                                                                                                <  1:3677931/150‑1 (MQ=255)
                                       atgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTg                                                                                                              >  2:1555244/1‑150 (MQ=255)
                                       atgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTg                                                                                                              <  2:1174408/150‑1 (MQ=255)
                                        tgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGATAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTgg                                                                                                             >  1:4375352/1‑150 (MQ=255)
                                                gCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAACGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGcc                                                                                                     >  2:4443951/1‑150 (MQ=255)
                                                     aaaaCGCGTGCGCCCAGCTAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTaaa                                                                                                <  2:3063089/150‑1 (MQ=255)
                                                      aaaCGCGTGCGCCAAGCTAAAAGCAGACCGGAAAGCAATCTAAGAAAGTACTGGCTAAACGTGCTGATAATAAAAAAGATAAAGAGAAAGCCACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAAc                                                                                               <  1:275497/150‑1 (MQ=255)
                                                       aaCGCGTGCGCCAAGCGAAAAGAAGACCGGCAAGCCATCTAAGAAAGTACTGGCTAAACGTGCTGATAAGAAAAAAGCTAAAGAGAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAAcc                                                                                              <  1:2956995/150‑1 (MQ=255)
                                                         cgcgTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCaa                                                                                            >  1:906986/1‑150 (MQ=255)
                                                           cgTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGc                                                                                          >  2:4349082/1‑150 (MQ=255)
                                                               cgcCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGaa                                                                                      >  2:731163/1‑150 (MQ=255)
                                                                gcCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAAc                                                                                     <  2:5128973/150‑1 (MQ=255)
                                                                 ccAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACg                                                                                    <  2:4229891/150‑1 (MQ=255)
                                                                    cgccaaaaGCAGACGGGAAAGCCATCTAAGAATTTACGGTCTAAACGTGCTGATAATAAAAAAGATAAATAGAAAGCGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGc                                                                                 <  1:2357372/146‑1 (MQ=255)
                                                                       taaaaGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACTTGCTTATAATAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTg                                                                              <  1:1271167/149‑1 (MQ=255)
                                                                       gAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTg                                                                              >  1:4584717/1‑150 (MQ=255)
                                                                        aaaaGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGc                                                                             >  1:4931024/1‑150 (MQ=255)
                                                                         aaaGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGcc                                                                            >  1:4315695/1‑150 (MQ=255)
                                                                             cAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCg                                                                        >  2:891187/1‑150 (MQ=255)
                                                                             cAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCg                                                                        >  2:891322/1‑150 (MQ=255)
                                                                               gACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCa                                                                      >  1:2218515/1‑150 (MQ=255)
                                                                                aCCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCaa                                                                     <  2:2591481/150‑1 (MQ=255)
                                                                                      aaGCCATCTAATAATGTAGTGGCTAACCGTGCTGATAATAAAAAAGATAACGACAAACACCCCCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACa                                                               <  1:1092945/150‑1 (MQ=255)
                                                                                      aaGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACa                                                               >  2:1975107/1‑150 (MQ=255)
                                                                                      aaGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACa                                                               >  1:3095261/1‑150 (MQ=255)
                                                                                         ccATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaa                                                            >  2:993028/1‑150 (MQ=255)
                                                                                          cATCTAAGAAAGTACTGTCTAAACGTGCTTAGAATAAAAAAGCTAAAGAGAAAGATAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaag                                                           <  1:1710057/150‑1 (MQ=255)
                                                                                          cATCGAAGAAATTACTGGCTAAACGTGCTGATAATAAAAAAGCTAAACACAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaag                                                           <  1:197986/150‑1 (MQ=255)
                                                                                          cATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaag                                                           >  1:2235321/1‑150 (MQ=255)
                                                                                           aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCACGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  2:3233011/1‑150 (MQ=255)
                                                                                           aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  1:1632529/1‑150 (MQ=255)
                                                                                           aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  2:5103590/1‑150 (MQ=255)
                                                                                           aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  1:4226792/1‑150 (MQ=255)
                                                                                           aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  1:3484845/1‑150 (MQ=255)
                                                                                           aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  2:2261422/1‑150 (MQ=255)
                                                                                            tctaagaaATTACTGGATAAACGTGCTGAGAATAAAAAATCTAAAGAGAAAGAGAAGCCGCGGGTTAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAagag                                                         <  1:3884724/147‑1 (MQ=255)
                                                                                             cGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGt                                                        >  1:3461945/1‑150 (MQ=255)
                                                                                              gaagaaAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCTCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGAGCCACCGCAAACGACAGAAGAGTa                                                       >  2:3926946/1‑150 (MQ=255)
                                                                                              gaagaaAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTa                                                       >  2:3926894/1‑150 (MQ=255)
                                                                                               aagaaTGTACTGGCTAACCGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTaa                                                      <  2:54684/150‑1 (MQ=255)
                                                                                               aagaaAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTaa                                                      >  2:76401/1‑150 (MQ=255)
                                                                                                 gaaAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtc                                                    <  2:3076127/150‑1 (MQ=255)
                                                                                                  aacgTACTGGATAAACGTGCTGATAATAAAAAAGCTAAAGACAAAGAGCACCCGCGGGTGAAAAAACCCAATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtct                                                   <  1:3901961/147‑1 (MQ=255)
                                                                                                  aaaGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtct                                                   >  2:2298788/1‑150 (MQ=255)
                                                                                                   aaGTACTGTCTAAACGTGCTGAGAATAAAAAAGCTAAATAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtctc                                                  <  2:1781638/150‑1 (MQ=255)
                                                                                                   aaGTACTGGCTAAACGTGCTTACAACAAAAAAGATAAATCGAAAGCCCCGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtctc                                                  <  1:965473/150‑1 (MQ=255)
                                                                                                   aaGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtctc                                                  >  2:3688177/1‑150 (MQ=255)
                                                                                                     ttACGGGCCAAACGTGGTGAGAATAAAAATGCTAAAGCCACCGCCACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAACCCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCaa                                                <  1:72540/149‑1 (MQ=255)
                                                                                                      tccTGGATAAAGGTGCTGATAATAAAAAAGCTAAAGAGAACCAGACGCCGCGGGGGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAAt                                               <  1:114842/148‑1 (MQ=255)
                                                                                                      tACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAAt                                               <  1:5030522/150‑1 (MQ=255)
                                                                                                      tACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAAt                                               <  2:4623594/150‑1 (MQ=255)
                                                                                                      tACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAAt                                               <  2:1613925/150‑1 (MQ=255)
                                                                                                              aatcGTGCTGAGAAGAAAAAAGCTAAAGAGATAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAACAGATTGGTAAGCGCCGTAAACCAAGCTGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGtt                                       <  2:4910587/147‑1 (MQ=255)
                                                                                                              aaaCGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGtt                                       >  1:81061/1‑150 (MQ=255)
                                                                                                               aaCGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGttt                                      <  2:4040356/150‑1 (MQ=255)
                                                                                                                    gCTGAGAATAAAAAAGATAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGcc                                 <  2:5009872/150‑1 (MQ=255)
                                                                                                                     cTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCt                                >  1:1764676/1‑150 (MQ=255)
                                                                                                                       gataataaaaaaGCTAAAGAGAACGCCACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTgg                              <  1:2408777/144‑1 (MQ=255)
                                                                                                                         gaagaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGACCGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTa                            >  2:1899126/1‑150 (MQ=255)
                                                                                                                           agaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTAtt                          <  2:794277/150‑1 (MQ=255)
                                                                                                                            gaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTa                         >  2:4686344/1‑150 (MQ=255)
                                                                                                                             aaaaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaa                        >  2:1502558/1‑150 (MQ=255)
                                                                                                                             aaaaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaa                        >  2:3663916/1‑150 (MQ=255)
                                                                                                                               aaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaaaa                      >  2:1490174/1‑150 (MQ=255)
                                                                                                                                  aGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGACAGGTGTAAGCCTGGTATTAAAAGTg                   >  1:4783657/1‑150 (MQ=255)
                                                                                                                                   gCTAACGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGc                  <  2:2110937/150‑1 (MQ=255)
                                                                                                                                   gCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGc                  <  2:2037745/150‑1 (MQ=255)
                                                                                                                                   gCTAAAGAGAAACAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGc                  <  2:622472/150‑1 (MQ=255)
                                                                                                                                      aaagagaaagagAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg               >  1:1491188/1‑150 (MQ=255)
                                                                                                                                      aaagagaaagagAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg               >  1:2280719/1‑150 (MQ=255)
                                                                                                                                       aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCCAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGGCATCTCAATGCCAGGTTTAAGCCTGGTAGTAAAAGTGCTGGc              >  2:4508783/1‑150 (MQ=255)
                                                                                                                                       aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGc              >  1:1573000/1‑150 (MQ=255)
                                                                                                                                       aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCAGGTATTAAAAGTGCTGGc              >  1:2552296/1‑150 (MQ=255)
                                                                                                                                        agagaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCa             >  2:1235365/1‑150 (MQ=255)
                                                                                                                                               gagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCaa      >  2:2114007/1‑150 (MQ=255)
                                                                                                                                                agaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCaaa     >  2:1709035/1‑150 (MQ=255)
                                                                                                                                                   aGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACt  <  2:1355615/150‑1 (MQ=255)
                                                                                                                                                   aGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACt  >  2:3246374/1‑150 (MQ=255)
                                                                                                                                                   aGCCGCGGGTGAAAAAACGCCAGCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACt  <  2:3485689/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACT  >  CP009273/2707345‑2707641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑