BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,811,408	T→A	5.0%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,811,408	0	T	A	5.0%	533.4 / 7.2	161	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (74/78); new base A (5/4); total (79/82)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  CP009273/2811259‑2811557

tctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                       >  1:2853292/2‑150 (MQ=255)

actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                       <  2:3879555/150‑1 (MQ=255)

actaAAGCCATATCAACTAAGAGATGGTGCATCCGGGAGGATTACTGGACTTGCGGCTCGCCCTACGGGCCGTTGCGAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                       <  2:755733/150‑1 (MQ=17)

  taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     <  1:3806032/150‑1 (MQ=255)

   aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg                                                                                                                                                    >  2:502735/1‑150 (MQ=255)

    aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   <  1:3507350/150‑1 (MQ=255)

    aaGCCATATCAATTAAGAGATGGTGCAACCGGGAGGATTACTCGCCATGCGGCTCGCCCTTCTGGCCGTTGCTAAAGCAACGG‑TATCATCCCTAGTGCCTGCGGATAATTCAGCTACCTCTTCGATACCATCATTGAAGAAGATGACGga                                                                                                                                                   >  2:4539776/1‑150 (MQ=255)

        cATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCACTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAat                                                                                                                                               <  1:4505487/150‑1 (MQ=255)

        cATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCCCAGAGCGCCTCGCCCTACTGGCCGTTTCTAAAGCCCCGC‑TGACATCCGTAGTGACAGCGGTTCATTCAGGTACCTCTTCGATACCTTCATTGCTGTAGATTTCGgcgatt                                                                                                                                               >  2:5006496/1‑148 (MQ=12)

          tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                             >  1:878774/1‑150 (MQ=255)

           atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                            >  2:1617924/1‑150 (MQ=255)

             cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                          <  2:111733/150‑1 (MQ=255)

              aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTAGGAGAATATGGTg                                                                                                                                         >  2:4824250/1‑150 (MQ=255)

                   agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATcc                                                                                                                                    <  1:4205683/150‑1 (MQ=255)

                   agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATcc                                                                                                                                    <  2:2558839/150‑1 (MQ=255)

                             cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAACGCAACGT‑TATCCTCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:2989955/150‑1 (MQ=255)

                             cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          >  2:4639211/1‑150 (MQ=255)

                             cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          >  1:3451415/1‑150 (MQ=255)

                              aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                         >  2:1624125/1‑150 (MQ=255)

                              aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                         >  1:1930353/1‑150 (MQ=255)

                              aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                         <  1:1492232/150‑1 (MQ=255)

                              aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                         >  1:3845593/1‑150 (MQ=255)

                              aTCCGGGAGGATTACTCGCCCTGCGGCTCGCCCTTCGGGCCGTTGCTAACGCAACGT‑TATCCTCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                         <  2:739944/150‑1 (MQ=255)

                                ccGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGaa                                                                                                                       >  2:4141156/1‑150 (MQ=255)

                                  gggAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAcc                                                                                                                     >  1:4670923/1‑150 (MQ=255)

                                    gaggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTc                                                                                                                   >  1:3520959/1‑150 (MQ=255)

                                     aggcTTACTCGCCCTGCGGCTCGCCCGTCGGGCCGTTGCTTAAGCAACGT‑TCTCATGCGTAGTGCCTGCGGATAATTCCGCTAACTCTTCTATACCTTAAGTGCTTAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                                                  <  1:4389513/150‑1 (MQ=17)

                                     aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                                                  >  2:3625345/1‑150 (MQ=255)

                                      ggaTGACTCGCCTTGCGGCTCGCCCGTCGGGCCGTTGCTACAGCAACGT‑TATCCTCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCg                                                                                                                 <  2:3493974/150‑1 (MQ=255)

                                         ttACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGGATCCGGGAGGATTCGAACCTCCGAcc                                                                                                              >  1:4802226/1‑150 (MQ=255)

                                           aCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTTCTAAAGC‑ACGTATATCATCCCTAGTGCCTGCGGTAAATTGCGCTACATGTTCGATACCTTCTATGCTGTAGATTTCGGAGAAGATGGTGCATCCGGGGGGATTCGAGCCTCCGACCGc                                                                                                            >  2:3240660/1‑150 (MQ=18)

                                           aCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGc                                                                                                            >  1:2057695/1‑150 (MQ=255)

                                               gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                        >  2:665237/1‑150 (MQ=255)

                                               gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                        >  2:3207723/1‑150 (MQ=255)

                                                  ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     <  1:1579730/150‑1 (MQ=255)

                                                   tGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                    >  2:1346360/1‑150 (MQ=255)

                                                         tCGCCCTTCGGGCCGTTGCTTTAGCAACGT‑TATCCTTCCTCGTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                              <  2:807055/150‑1 (MQ=11)

                                                           gCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg                                                                                            >  1:2924717/1‑150 (MQ=255)

                                                           gCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg                                                                                            <  1:502735/150‑1 (MQ=255)

                                                                 cGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTAAATTGATGAATAATTGGGTGAAGATGGTGCGTGCGGGAGGATTCGATGCTCCGTCGGCGGGGGTGGTAGCGGCGTActct                                                                                      >  1:1052790/1‑150 (MQ=12)

                                                                 cGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                                                                      >  2:4987421/1‑150 (MQ=255)

                                                                  gggCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                     >  1:4164635/1‑150 (MQ=255)

                                                                  gggCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                     >  1:2620847/1‑150 (MQ=255)

                                                                  gggCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                     >  1:640270/1‑150 (MQ=255)

                                                                          gCTCAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                             <  2:1993921/150‑1 (MQ=255)

                                                                          gCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                             >  1:2295829/1‑150 (MQ=255)

                                                                            tAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           >  2:559023/1‑150 (MQ=255)

                                                                            tAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           <  1:4987421/150‑1 (MQ=255)

                                                                            tAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           >  2:559027/1‑150 (MQ=255)

                                                                              aaGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAAGTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                         <  1:547348/150‑1 (MQ=255)

                                                                                   aCGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                                    <  2:46596/150‑1 (MQ=255)

                                                                                   aCGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                                    <  2:46647/150‑1 (MQ=255)

                                                                                     gT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                  <  2:837391/150‑1 (MQ=255)

                                                                                     gT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                  >  1:998525/1‑150 (MQ=255)

                                                                                     gT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                  <  1:3017292/150‑1 (MQ=255)

                                                                                          tcatcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                                                              >  2:4827389/1‑150 (MQ=255)

                                                                                                 tAGTGCCTGCGGTTAAGTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                       <  2:2922385/150‑1 (MQ=255)

                                                                                                     gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttat                                                   <  1:3887957/150‑1 (MQ=255)

                                                                                                        tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAACTTTAtttt                                                >  2:2731976/1‑150 (MQ=255)

                                                                                                        tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTActtt                                                >  1:3212083/1‑150 (MQ=255)

                                                                                                               cataccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                         <  2:3921489/146‑1 (MQ=255)

                                                                                                               aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                         >  2:3085544/1‑150 (MQ=255)

                                                                                                               aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                         <  1:3893879/150‑1 (MQ=255)

                                                                                                               aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                         <  2:445588/150‑1 (MQ=255)

                                                                                                                 ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGat                                       >  2:4844162/1‑150 (MQ=255)

                                                                                                                 ttCCGCTACCTCTTCGATACCGTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGat                                       <  2:5157110/150‑1 (MQ=255)

                                                                                                                  tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGata                                      <  2:3114855/150‑1 (MQ=255)

                                                                                                                  tCCGCGACCTCTTCGATACCTTCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGata                                      <  2:180381/150‑1 (MQ=255)

                                                                                                                   ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTTGGGGTGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGatat                                     <  1:2084704/150‑1 (MQ=255)

                                                                                                                    cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                    >  1:1601868/1‑150 (MQ=255)

                                                                                                                    cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                    <  2:3520959/150‑1 (MQ=255)

                                                                                                                    cGCTACCTCTTCGATACCTTCATGGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                    <  2:2940197/150‑1 (MQ=255)

                                                                                                                       tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttttttg                                 >  2:1173206/1‑147 (MQ=14)

                                                                                                                         ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAc                               >  1:2416216/1‑150 (MQ=255)

                                                                                                                         ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAc                               <  1:1513723/150‑1 (MQ=255)

                                                                                                                         ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTATACTGCTGATATTGATaa                               >  1:3047915/1‑149 (MQ=255)

                                                                                                                          ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcc                              >  2:2853345/1‑150 (MQ=255)

                                                                                                                          ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcc                              <  1:4697633/150‑1 (MQ=255)

                                                                                                                             ttCGATACCTTCCTTGCTGAAGATTTCGGAGAATAGGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                           <  2:2275246/150‑1 (MQ=255)

                                                                                                                             ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                           <  1:169973/150‑1 (MQ=255)

                                                                                                                              tctttACCTTCATTGCTGTAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                          <  1:3249493/146‑1 (MQ=255)

                                                                                                                              tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                          <  1:4585868/150‑1 (MQ=255)

                                                                                                                               cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctac                         >  1:4105358/1‑150 (MQ=255)

                                                                                                                                  tACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGATATTTATTTTACTGCTGATATTGATAc                               >  1:3348023/1‑141 (MQ=16)

                                                                                                                                  tACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGATATTTATTTTACTGCTGATATTGATAc                               <  2:3348023/141‑1 (MQ=16)

                                                                                                                                    ccTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATATATTTAACAGCTTAAATTGATACCCCAACAAAAg                    >  2:2576225/1‑150 (MQ=11)

                                                                                                                                      ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                >  2:2743280/1‑120 (MQ=9)

                                                                                                                                      ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                <  1:2743280/120‑1 (MQ=9)

                                                                                                                                          ttGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                  <  2:2329206/98‑1 (MQ=37)

                                                                                                                                          ttGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAGTTTACTGCTGATATTGATAACGCTACAAAAGCCaaat              >  1:3661701/1‑150 (MQ=11)

                                                                                                                                           tGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATc             <  1:2576225/150‑1 (MQ=25)

                                                                                                                                           tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATc             <  1:1252692/150‑1 (MQ=255)

                                                                                                                                             cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTCCTAAAGCCATATCaa           >  1:1911232/1‑150 (MQ=255)

                                                                                                                                              tGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          <  1:2691575/150‑1 (MQ=255)

                                                                                                                                              tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          <  1:4141156/150‑1 (MQ=255)

                                                                                                                                               gAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATATATTTAACATCTGATATTGTTTCCGCAAATAAAGCCATTTCAAga         >  2:2691575/1‑149 (MQ=11)

                                                                                                                                                aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:3733877/36‑1 (MQ=255)

                                                                                                                                                  gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTCTTTTACTGCAGATATTTATACCGCGCCTCAAGCCAGATCAAGTCag      >  2:3198576/1‑150 (MQ=14)

                                                                                                                                                     ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAgaga   <  2:1096804/150‑1 (MQ=255)

                                                                                                                                                      tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt  >  1:4524526/1‑150 (MQ=255)

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGT‑TATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  CP009273/2811259‑2811557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`