Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,901,394 C→A 20.2% A544A (GCG→GCT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,901,3940CA20.2% 555.6 / 105.5 209A544A (GCG→GCT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (81/85);  new base A (22/21);  total (103/106)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATC  >  CP009273/2901245‑2901543
                                                                                                                                                     |                                                                                                                                                     
   ttACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACTATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGAAAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGcc                                                                                                                                                    <  1:2866180/150‑1 (MQ=255)
       cACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTAACCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGAc                                                                                                                                                >  1:1980742/1‑150 (MQ=255)
          gggAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATGAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGtt                                                                                                                                             <  2:620066/150‑1 (MQ=255)
             tgTCGATGTTTTCACGACCGATTATTTTTACGATTTGGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACACCGCGTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCt                                                                                                                                          <  1:663053/149‑1 (MQ=255)
             aGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCt                                                                                                                                          <  2:188226/150‑1 (MQ=255)
                cGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGa                                                                                                                                       >  2:4896820/1‑150 (MQ=255)
                 gatgatTTCACGACCGATGATTTTTACGATTTTGGAAATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGACGCTCTAACTTTTTTACTTACTTCTCATGACGTTTCTGGaa                                                                                                                                      >  2:42920/1‑150 (MQ=255)
                  atgatTTCACGACCGATGAATTTTCCGATTTTGGACATTAGGTGTTCCGCAAGTCACTAGTTAAACTTAAACGCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCGAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAAc                                                                                                                                     <  2:3052132/150‑1 (MQ=255)
                  atgagTTCACGACCGATGATTTGTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACGCCAGACAAACCACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAAc                                                                                                                                     <  2:4339675/150‑1 (MQ=255)
                   tgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACt                                                                                                                                    >  1:1605370/1‑150 (MQ=255)
                   tgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACt                                                                                                                                    >  2:4455111/1‑150 (MQ=255)
                        tCACGACCGATGATTTTTCCGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACGTATCCTGACGTTTCTGGAACTCGCTg                                                                                                                               >  2:2410809/1‑150 (MQ=255)
                        tCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTg                                                                                                                               >  1:4937886/1‑150 (MQ=255)
                         cACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTgg                                                                                                                              >  2:1653422/1‑150 (MQ=255)
                            gACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcgg                                                                                                                           >  1:2144741/1‑150 (MQ=255)
                            gACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcgg                                                                                                                           >  1:4284515/1‑150 (MQ=255)
                              ccgtttatTTTTCCGATTTTGTAAATTTGGTTTACCTCAAGTCACTAGTTAAACTTAAACTCCAGACAAACACCGCGTCCCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt                                                                                                                         <  1:2300954/144‑1 (MQ=255)
                              ccGATGTTTTTTACGATTTTGGACCTTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt                                                                                                                         <  2:1291818/150‑1 (MQ=255)
                               ctatgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCtt                                                                                                                        <  1:1138231/148‑1 (MQ=255)
                               cGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTATTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCtt                                                                                                                        >  2:4510789/1‑150 (MQ=255)
                                gatgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAACCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCtta                                                                                                                       <  1:1620629/150‑2 (MQ=255)
                                 atgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTc                                                                                                                      >  1:4810449/1‑150 (MQ=255)
                                       tttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAg                                                                                                                >  2:3702138/1‑150 (MQ=255)
                                         tACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGcc                                                                                                              <  1:3587204/150‑1 (MQ=255)
                                           cGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTGTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg                                                                                                            >  2:4116454/1‑150 (MQ=255)
                                           cGAGTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg                                                                                                            <  2:3403883/150‑1 (MQ=255)
                                            gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                                                           >  1:4633003/1‑150 (MQ=255)
                                            gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTATTTTACTAACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                                                           >  1:4632826/1‑150 (MQ=255)
                                             aTTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCa                                                                                                          >  1:195198/1‑150 (MQ=255)
                                             aTTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCa                                                                                                          >  1:1567755/1‑150 (MQ=255)
                                              ttttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCaa                                                                                                         <  2:2295009/150‑1 (MQ=255)
                                              ttttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCaa                                                                                                         >  1:2703219/1‑150 (MQ=255)
                                                ttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAAc                                                                                                       >  2:262539/1‑150 (MQ=255)
                                                 tGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACa                                                                                                      <  2:2721210/150‑1 (MQ=255)
                                                  ggACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAg                                                                                                     <  1:4417297/150‑1 (MQ=255)
                                                    aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                                                                   <  1:2929518/150‑1 (MQ=255)
                                                    aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                                                                   >  1:2036724/1‑150 (MQ=255)
                                                     cATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg                                                                                                  >  2:3529699/1‑150 (MQ=255)
                                                      aTTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg                                                                                                 <  2:1757912/150‑1 (MQ=255)
                                                         aGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGa                                                                                              <  2:883972/150‑1 (MQ=255)
                                                           gTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAcc                                                                                            >  1:4916745/1‑150 (MQ=255)
                                                           gTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACGTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAcc                                                                                            <  2:4213067/150‑1 (MQ=255)
                                                             tttCCTCAAGTCACTTGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGTTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCAt                                                                                          <  1:942912/150‑1 (MQ=255)
                                                               accTCAAGTCACTAGTTACACAGACACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCAGCTCTAAAATTTTTACTTACTTCTCCTGACGTTTATGTAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACTATCa                                                                                        <  2:3919314/149‑1 (MQ=255)
                                                                ccTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc                                                                                       <  2:1077087/150‑1 (MQ=255)
                                                                    aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                                                                   <  2:1585461/150‑1 (MQ=255)
                                                                    aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                                                                   <  1:3729052/150‑1 (MQ=255)
                                                                    aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGGACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                                                                   <  1:3857351/150‑1 (MQ=255)
                                                                     aGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGa                                                                                  <  2:812282/150‑1 (MQ=255)
                                                                      gTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg                                                                                 >  2:1303632/1‑150 (MQ=255)
                                                                       tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGt                                                                                <  2:3123758/150‑1 (MQ=255)
                                                                       tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGt                                                                                >  2:1129804/1‑150 (MQ=255)
                                                                         attatttAAACTTAACCTCAACACAAACACCGCGTACCCCGGGTAGGCGTTGCCGCTCTAACATTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAg                                                                              <  1:42920/145‑1 (MQ=255)
                                                                         aCTAGTTAAACTGAAACTCCAGACACACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAg                                                                              <  2:4792050/150‑1 (MQ=255)
                                                                            aGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAg                                                                           >  2:1047255/1‑150 (MQ=255)
                                                                             gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt                                                                          <  2:2871777/150‑1 (MQ=255)
                                                                             gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt                                                                          <  2:1636810/150‑1 (MQ=255)
                                                                                 aaCTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAAc                                                                      <  1:4469970/150‑1 (MQ=255)
                                                                                  aCTGAAACTCCAGACAACCAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt                                                                     <  1:4582094/150‑1 (MQ=255)
                                                                                  aCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt                                                                     <  1:1520209/150‑1 (MQ=255)
                                                                                  aCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt                                                                     >  1:982287/1‑150 (MQ=255)
                                                                                  aCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACGTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt                                                                     <  2:1158/150‑1 (MQ=255)
                                                                                       aaCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa                                                                >  1:1419587/1‑150 (MQ=255)
                                                                                       aaCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa                                                                >  1:4017936/1‑150 (MQ=255)
                                                                                          tCCAGACAAACACCGCGTCCCCCGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATgg                                                             <  1:1963706/150‑1 (MQ=255)
                                                                                             aGACAAACACCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                                                          <  1:3233795/150‑1 (MQ=255)
                                                                                             aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                                                          <  2:3360599/150‑1 (MQ=255)
                                                                                             aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTCGAGTAGAAGTAAACTCCGGATGGAAc                                                          >  2:3759436/1‑150 (MQ=255)
                                                                                             aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                                                          <  1:4537844/150‑1 (MQ=255)
                                                                                                     aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc                                                  >  1:2972163/1‑150 (MQ=255)
                                                                                                      acGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGcc                                                 <  1:3229985/150‑1 (MQ=255)
                                                                                                             cccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCCTCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc                                          >  1:2402446/1‑150 (MQ=255)
                                                                                                             cccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc                                          >  1:2402425/1‑150 (MQ=255)
                                                                                                              ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCa                                         >  1:142166/1‑150 (MQ=255)
                                                                                                              ccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCa                                         >  1:294598/1‑150 (MQ=255)
                                                                                                                 gggTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACgg                                      >  1:3651944/1‑150 (MQ=255)
                                                                                                                   gTACGCGTTGCCGCTCTAACTTTTTTACTTACTTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGt                                    >  1:621223/1‑150 (MQ=255)
                                                                                                                    tACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTg                                   <  1:440337/150‑1 (MQ=255)
                                                                                                                      cgcgTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGAt                                 >  2:2279318/1‑150 (MQ=255)
                                                                                                                          ttGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCgg                             <  2:75712/150‑1 (MQ=255)
                                                                                                                           tGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGa                            <  1:4768751/150‑1 (MQ=255)
                                                                                                                            gCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGaa                           >  2:3800243/1‑150 (MQ=255)
                                                                                                                            gCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCAACACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGaa                           >  1:2221320/1‑150 (MQ=255)
                                                                                                                             ccGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAAc                          <  1:678941/150‑1 (MQ=255)
                                                                                                                             ccGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAAc                          >  1:273958/1‑150 (MQ=255)
                                                                                                                              cGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACt                         >  1:3895084/1‑150 (MQ=255)
                                                                                                                                   tAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgat                    <  2:1455208/150‑1 (MQ=255)
                                                                                                                                   tAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgat                    >  1:3520409/1‑150 (MQ=255)
                                                                                                                                    aaCTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatg                   >  2:4052308/1‑150 (MQ=255)
                                                                                                                                      cTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatgat                 >  2:2035744/1‑150 (MQ=255)
                                                                                                                                       ttttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGAtc                <  2:2447478/150‑1 (MQ=255)
                                                                                                                                           ttacttacTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGa            >  2:2549330/1‑150 (MQ=255)
                                                                                                                                            tacttactTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGAc           <  1:2842450/150‑1 (MQ=255)
                                                                                                                                            tacttactTAGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGAc           <  1:4801199/150‑1 (MQ=255)
                                                                                                                                             acttacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGAcc          <  1:4688494/150‑1 (MQ=255)
                                                                                                                                              cttacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCa         <  1:2650714/150‑1 (MQ=255)
                                                                                                                                                 acttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACt      <  1:1852545/150‑1 (MQ=255)
                                                                                                                                                  cttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTg     >  2:4995680/1‑150 (MQ=255)
                                                                                                                                                     cGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGatc  <  1:3144580/150‑1 (MQ=255)
                                                                                                                                                     cGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGatc  <  2:5039345/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATC  >  CP009273/2901245‑2901543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑