BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,020,489	G→T	13.6%	D4Y (GAC→TAC)	ygfQ →	putative purine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,020,489	0	G	T	13.6%	686.5 / 67.8	212	D4Y (GAC→TAC)	ygfQ	putative purine permease
Reads supporting (aligned to +/- strand): ref base G (99/84); new base T (11/18); total (110/102)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCG  >  CP009273/3020341‑3020638

ctGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGgaga                                                                                                                                                      <  1:4558749/150‑1 (MQ=255)

 tGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGAc                                                                                                                                                     >  1:3640452/1‑150 (MQ=255)

 tGTTTCTTTAGTCGAAAAATTGTTCGTGATGAAGACGTTGGGCGATGACCGTTCTATCTACCGCACCTACGTTGATGTTCGTATGGTGTACGAACGCAACTAATAATAAAAATTTAACATCCTCTTGAGGACATCATAATGTCTGGAGAc                                                                                                                                                     >  2:1306722/1‑150 (MQ=255)

  gTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACa                                                                                                                                                    <  2:1996001/150‑1 (MQ=255)

     tCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATcc                                                                                                                                                 <  2:584623/150‑1 (MQ=255)

     tCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATcc                                                                                                                                                 >  2:2842985/1‑150 (MQ=255)

      cTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCt                                                                                                                                                >  1:4113767/1‑150 (MQ=255)

        ttAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTAc                                                                                                                                              >  1:3702333/1‑150 (MQ=255)

         tAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACa                                                                                                                                             >  2:3837784/1‑150 (MQ=255)

            tCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAac                                                                                                                                          <  1:4066962/150‑1 (MQ=255)

              gACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAacac                                                                                                                                        >  1:2268067/1‑150 (MQ=255)

                cAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCg                                                                                                                                      >  1:2065728/1‑150 (MQ=255)

                cAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCg                                                                                                                                      >  1:4174921/1‑150 (MQ=255)

                cAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCg                                                                                                                                      >  1:3400526/1‑150 (MQ=255)

                 aaaTTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCgg                                                                                                                                     <  2:1595736/150‑1 (MQ=255)

                 aaaTTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCgg                                                                                                                                     >  2:26643/1‑150 (MQ=255)

                   aTTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGAc                                                                                                                                   >  2:1615216/1‑150 (MQ=255)

                      gTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCa                                                                                                                                <  2:1210246/150‑1 (MQ=255)

                       ttCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCAc                                                                                                                               <  1:636664/150‑1 (MQ=255)

                         cGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCa                                                                                                                             >  1:1172234/1‑150 (MQ=255)

                         cGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCa                                                                                                                             >  2:1391190/1‑150 (MQ=255)

                         cGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCa                                                                                                                             >  2:3885931/1‑150 (MQ=255)

                         cGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCa                                                                                                                             >  2:3886086/1‑150 (MQ=255)

                          gTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCaa                                                                                                                            <  2:1510626/150‑1 (MQ=255)

                          gTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCaa                                                                                                                            <  2:4105721/150‑1 (MQ=255)

                            gatgatgaCGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAg                                                                                                                          >  2:4890465/1‑150 (MQ=255)

                             atgatgaCGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGc                                                                                                                         >  1:4499419/1‑150 (MQ=255)

                               gatgaCGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCca                                                                                                                       >  2:208364/1‑150 (MQ=255)

                               gatgaCGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCca                                                                                                                       >  2:1087954/1‑150 (MQ=255)

                                   aCGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAgg                                                                                                                   <  2:2375215/150‑1 (MQ=255)

                                     gTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGgc                                                                                                                 <  2:288978/150‑1 (MQ=255)

                                         ggCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGgcgcgc                                                                                                             <  2:706207/150‑1 (MQ=255)

                                           cGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTg                                                                                                           <  2:4665512/150‑1 (MQ=255)

                                            gATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTgg                                                                                                          <  2:423279/150‑1 (MQ=255)

                                             aTGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGa                                                                                                         <  1:4160348/150‑1 (MQ=255)

                                             aTGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGa                                                                                                         <  2:2112334/150‑1 (MQ=255)

                                             aTGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTGAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGa                                                                                                         <  2:2764120/150‑1 (MQ=255)

                                               gACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATa                                                                                                       >  1:1267707/1‑150 (MQ=255)

                                                 ccGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAat                                                                                                     >  1:1940228/1‑150 (MQ=255)

                                                 ccGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAat                                                                                                     >  1:629699/1‑150 (MQ=255)

                                                      cGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTAttt                                                                                                <  2:2804145/150‑1 (MQ=255)

                                                         tCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTaa                                                                                             >  2:1747703/1‑150 (MQ=255)

                                                          cTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTaaa                                                                                            >  2:2443138/1‑150 (MQ=255)

                                                               gCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTAc                                                                                       <  2:1688353/150‑1 (MQ=255)

                                                                cACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTAcc                                                                                      <  1:3080381/150‑1 (MQ=255)

                                                                 aCCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCg                                                                                     <  2:606141/150‑1 (MQ=255)

                                                                 aCCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCg                                                                                     >  1:2128654/1‑150 (MQ=255)

                                                                     aCGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCg                                                                                 <  2:2290439/150‑1 (MQ=255)

                                                                     aCGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCg                                                                                 <  2:2290651/150‑1 (MQ=255)

                                                                     aCGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCg                                                                                 <  2:340894/150‑1 (MQ=255)

                                                                     aCGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCg                                                                                 <  1:503373/150‑1 (MQ=255)

                                                                      cGTTGATGGTCGTCTGGTGTACGAACTCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGt                                                                                >  1:2352072/1‑150 (MQ=255)

                                                                         tGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGc                                                                             <  1:2042761/150‑1 (MQ=255)

                                                                         tGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGc                                                                             >  2:317394/1‑150 (MQ=255)

                                                                         tGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGc                                                                             >  2:3802861/1‑150 (MQ=255)

                                                                             ggTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTa                                                                         >  2:4743999/1‑150 (MQ=255)

                                                                                  tcTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGtt                                                                    >  2:4266592/1‑150 (MQ=255)

                                                                                     ggTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGt                                                                 >  2:4582002/1‑150 (MQ=255)

                                                                                     ggTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGt                                                                 <  1:5053404/150‑1 (MQ=255)

                                                                                        gtACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAg                                                              >  2:1799641/1‑150 (MQ=255)

                                                                                          aCGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGa                                                            >  2:4062234/1‑150 (MQ=255)

                                                                                               cGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTAc                                                       >  2:3713593/1‑150 (MQ=255)

                                                                                               cGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTAc                                                       <  1:2961989/150‑1 (MQ=255)

                                                                                               cGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTAc                                                       >  1:935744/1‑150 (MQ=255)

                                                                                                gCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTAct                                                      <  2:1829937/150‑1 (MQ=255)

                                                                                                 cAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTActg                                                     <  2:3625299/150‑1 (MQ=255)

                                                                                                   aCTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTActggc                                                   >  2:1183836/1‑150 (MQ=255)

                                                                                                   aCTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTActggc                                                   >  1:456917/1‑150 (MQ=255)

                                                                                                    cTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCGGTACCGTTCGTCAGGAAGTActggct                                                  <  1:2159658/150‑1 (MQ=255)

                                                                                                       ataataaAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACtggctggc                                               >  1:4851198/1‑150 (MQ=255)

                                                                                                            aaaaCTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAAc                                          <  1:1484219/150‑1 (MQ=255)

                                                                                                             aaaCTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACg                                         <  1:498480/150‑1 (MQ=255)

                                                                                                               aCTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGAc                                       >  1:283364/1‑150 (MQ=255)

                                                                                                                cTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGAcc                                      <  2:2553285/150‑1 (MQ=255)

                                                                                                                   tAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCttt                                   <  1:2759954/150‑1 (MQ=255)

                                                                                                                     aCATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCt                                 <  1:3713036/150‑1 (MQ=255)

                                                                                                                        tCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGc                              >  2:1329239/1‑150 (MQ=255)

                                                                                                                         ccTCGTGAGGACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGcc                             <  1:1714305/150‑1 (MQ=255)

                                                                                                                            cGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATg                          >  1:2677048/1‑150 (MQ=255)

                                                                                                                            cGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATg                          >  1:2311791/1‑150 (MQ=255)

                                                                                                                             gTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATgg                         <  1:247145/150‑1 (MQ=255)

                                                                                                                                aGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGttt                      <  1:898007/150‑1 (MQ=255)

                                                                                                                                 ggACATCATTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTa                     <  1:384337/150‑1 (MQ=255)

                                                                                                                                 ggACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTa                     <  1:583785/150‑1 (MQ=255)

                                                                                                                                 ggACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTa                     >  2:2408557/1‑150 (MQ=255)

                                                                                                                                  gACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTAt                    >  2:5058672/1‑150 (MQ=255)

                                                                                                                                   aCATCATTATGTCTGGATACATCCTACAAACACCGGACGCAACAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTAtt                   >  2:2290505/1‑150 (MQ=255)

                                                                                                                                     atcatTATGTCTGGATACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTcc                 >  1:4032950/1‑150 (MQ=255)

                                                                                                                                     atcatTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTcc                 >  1:746789/1‑150 (MQ=255)

                                                                                                                                     atcatTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTcc                 >  2:2783954/1‑150 (MQ=255)

                                                                                                                                       catTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGt               >  1:1764357/1‑150 (MQ=255)

                                                                                                                                          tATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAt            <  1:1763790/150‑1 (MQ=255)

                                                                                                                                          tATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAt            >  1:1307556/1‑150 (MQ=255)

                                                                                                                                           aTGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtc           <  2:412163/150‑1 (MQ=255)

                                                                                                                                              tCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgtc        <  2:671764/150‑1 (MQ=255)

                                                                                                                                              tCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgtc        <  2:1835265/150‑1 (MQ=255)

                                                                                                                                                 ggAGACATCCCACAACCACCGGACGCACCACAGCCACCGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACAGGTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGtt     <  2:3536391/150‑1 (MQ=255)

                                                                                                                                                  gagaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTc    >  1:4549433/1‑150 (MQ=255)

                                                                                                                                                    gaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCg  >  1:1491648/1‑150 (MQ=255)

                                                                                                                                                    gaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGGTATCGTCGTTCCg  <  1:1151949/150‑1 (MQ=255)

CTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTTGGGCGATGACCGTTCGATCTACCGCACCTACGTTGATGGTCGTCTGGTGTACGAACGCAACTAATAATAAAACTTTAACATCCTCGTGAGGACATCATTATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCG  >  CP009273/3020341‑3020638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`