Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,020,629 C→A 11.1% I50I (ATC→ATA)  ygfQ → putative purine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,020,6290CA11.1% 832.4 / 64.1 249I50I (ATC→ATA) ygfQputative purine permease
Reads supporting (aligned to +/- strand):  ref base C (134/87);  new base A (17/11);  total (151/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

ATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACGGCG  >  CP009273/3020480‑3020776
                                                                                                                                                     |                                                                                                                                                   
aTGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtc                                                                                                                                                     <  1:1190165/150‑1 (MQ=255)
aTGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtc                                                                                                                                                     <  2:412163/150‑1 (MQ=255)
   tCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgtc                                                                                                                                                  <  2:671764/150‑1 (MQ=255)
   tCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTAtcgtc                                                                                                                                                  <  2:1835265/150‑1 (MQ=255)
      ggAGACATCCCACAACCACCGGACGCACCACAGCCACCGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACAGGTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGtt                                                                                                                                               <  2:3536391/150‑1 (MQ=255)
         gaCATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCg                                                                                                                                            <  1:1151994/150‑1 (MQ=255)
            aTCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGa                                                                                                                                         >  2:308544/1‑150 (MQ=255)
            aTCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGa                                                                                                                                         <  2:4465209/150‑1 (MQ=255)
               cTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGACTg                                                                                                                                      >  1:3138775/1‑150 (MQ=255)
               cTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATg                                                                                                                                      >  1:4296818/1‑150 (MQ=255)
                tACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGc                                                                                                                                     >  1:4505737/1‑150 (MQ=255)
                  cAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTg                                                                                                                                   >  1:1025048/1‑150 (MQ=255)
                     acacCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGc                                                                                                                                >  2:1395779/1‑150 (MQ=255)
                         cGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTGTCTGGCCAGGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAg                                                                                                                            >  2:2220332/1‑150 (MQ=255)
                          ggACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGc                                                                                                                           >  1:1385651/1‑150 (MQ=255)
                             cGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAgg                                                                                                                        <  1:3032027/150‑1 (MQ=255)
                              gCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCTTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGt                                                                                                                       >  2:5050435/1‑150 (MQ=255)
                              gCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGt                                                                                                                       >  2:2421281/1‑150 (MQ=255)
                               cACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATNCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGtt                                                                                                                      <  1:4793816/150‑1 (MQ=255)
                                aCCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGttt                                                                                                                     >  2:3804668/1‑150 (MQ=255)
                                  cAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtc                                                                                                                   <  1:958248/150‑1 (MQ=255)
                                     aGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTtcctc                                                                                                                >  2:327453/1‑150 (MQ=255)
                                        cacaGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCg                                                                                                             <  2:56031/150‑1 (MQ=255)
                                             ggCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCt                                                                                                        >  1:596390/1‑150 (MQ=255)
                                             ggCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCt                                                                                                        >  1:4882552/1‑150 (MQ=255)
                                                   cTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgttt                                                                                                  >  2:3421093/1‑150 (MQ=255)
                                                    tGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttg                                                                                                 >  2:1229767/1‑150 (MQ=255)
                                                    tGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAACTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGATATCGTCGTTCCGGGAATGATGGGCAAAGCAGGTTTTCCTCCCGCAGCGGtgtttg                                                                                                 >  2:4298976/1‑150 (MQ=255)
                                                      gATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGGATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGtgtttgtt                                                                                               <  1:5014082/150‑1 (MQ=255)
                                                       aTAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTttgttg                                                                                              >  1:3601784/1‑150 (MQ=255)
                                                             attTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCt                                                                                        >  2:4406141/1‑150 (MQ=255)
                                                                 tAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCt                                                                                    <  1:4581947/150‑1 (MQ=255)
                                                                    aaTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGt                                                                                 <  1:1113/150‑1 (MQ=255)
                                                                    aaTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGt                                                                                 >  1:2996163/1‑150 (MQ=255)
                                                                    aaTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGt                                                                                 >  1:3146190/1‑150 (MQ=255)
                                                                     aTTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTc                                                                                >  1:4270257/1‑150 (MQ=255)
                                                                       tACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTcgc                                                                              >  1:491348/1‑150 (MQ=255)
                                                                       tACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGATTAACTACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCAGCAGCTGTGTTTGTTGCCACCTGTCTGGtaga                                                                              >  1:4121164/1‑147 (MQ=255)
                                                                        aCCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTcgcg                                                                             >  1:1489389/1‑150 (MQ=255)
                                                                         ccGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCgg                                                                            >  2:1504425/1‑150 (MQ=255)
                                                                            cTCGTGGCAGTACCGTTCGTCAGGTAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCt                                                                         <  2:4251961/150‑1 (MQ=255)
                                                                             tCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCtt                                                                        <  1:3120107/150‑1 (MQ=255)
                                                                               gTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCg                                                                      >  1:4458708/1‑150 (MQ=255)
                                                                                tGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCgg                                                                     >  1:571829/1‑150 (MQ=255)
                                                                                 ggCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGc                                                                    >  1:714003/1‑150 (MQ=255)
                                                                                    aGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCg                                                                 <  2:2180704/150‑1 (MQ=255)
                                                                                     gTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGt                                                                >  2:3992817/1‑150 (MQ=255)
                                                                                     gTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGt                                                                >  2:2267852/1‑150 (MQ=255)
                                                                                      tACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGtt                                                               <  2:2515710/150‑1 (MQ=255)
                                                                                      tACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGtt                                                               <  2:2065728/150‑1 (MQ=255)
                                                                                       aCCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTg                                                              >  1:4411217/1‑150 (MQ=255)
                                                                                       aCCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTg                                                              >  2:2657356/1‑150 (MQ=255)
                                                                                          gTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTg                                                           >  1:2586707/1‑150 (MQ=255)
                                                                                          gTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTg                                                           >  2:4698283/1‑150 (MQ=255)
                                                                                           ttCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTAGTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGa                                                          <  2:2272001/150‑1 (MQ=255)
                                                                                            tCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGAt                                                         <  1:1391190/150‑1 (MQ=255)
                                                                                            tCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGAt                                                         >  1:2293640/1‑150 (MQ=255)
                                                                                            tCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGAt                                                         <  1:2793206/150‑1 (MQ=255)
                                                                                            tCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGAt                                                         <  1:3885931/150‑1 (MQ=255)
                                                                                                 aGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGAt                                                    >  2:4899736/1‑150 (MQ=255)
                                                                                                    aaGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTAt                                                 <  2:3244015/150‑1 (MQ=255)
                                                                                                      gTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTACGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATgg                                               >  2:4835880/1‑150 (MQ=255)
                                                                                                       tACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATggg                                              <  2:3670760/150‑1 (MQ=255)
                                                                                                       tACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATggg                                              <  1:4219138/150‑1 (MQ=255)
                                                                                                         ctggctggCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCt                                            <  1:1576934/150‑1 (MQ=255)
                                                                                                               ggctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTg                                      >  2:256009/1‑150 (MQ=255)
                                                                                                               ggctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTg                                      >  2:4795810/1‑150 (MQ=255)
                                                                                                                 ctTAACGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGcc                                    <  1:2223551/150‑1 (MQ=255)
                                                                                                                  ttAACGACCTTTCTGGCCATGTTTTATTCCGTTATCGTCGTTCCGGTAATGCTGGGCAAAGCAGGTTTTCCTCACGCAGCTGTGATTTTATACACCTGTCTTTTAGAGGGATTCTGATCGTTGCTGATGGGATTATTGGCTAATATTCCa                                   >  2:2826376/1‑150 (MQ=255)
                                                                                                                   tAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCaa                                  >  2:1785096/1‑150 (MQ=255)
                                                                                                                   tAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCaa                                  >  2:5096417/1‑150 (MQ=255)
                                                                                                                     aCGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAAAGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATg                                >  2:1906875/1‑150 (MQ=255)
                                                                                                                      cGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCGGATGGGATTATGGGCTAATTTGCCAATgg                               >  1:2490693/1‑150 (MQ=255)
                                                                                                                      cGACCTTTCTGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATgg                               <  1:2023902/150‑1 (MQ=255)
                                                                                                                       gACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGc                              <  1:4740755/150‑1 (MQ=255)
                                                                                                                         ccTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGa                            >  1:4636784/1‑150 (MQ=255)
                                                                                                                           tttCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGAtt                          >  2:838707/1‑150 (MQ=255)
                                                                                                                             tCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTgg                        <  1:2783954/150‑1 (MQ=255)
                                                                                                                              cTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGt                       <  2:629699/150‑1 (MQ=255)
                                                                                                                              cTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGt                       <  2:1940228/150‑1 (MQ=255)
                                                                                                                               tGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTTCCAATGGCGATTGGtt                      >  1:3982063/1‑150 (MQ=255)
                                                                                                                               tGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGtt                      >  1:3982102/1‑150 (MQ=255)
                                                                                                                               tGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGtt                      >  1:3434758/1‑150 (MQ=255)
                                                                                                                               tGGCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGtt                      <  2:4766277/150‑1 (MQ=255)
                                                                                                                                ggCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTg                     >  1:2074539/1‑150 (MQ=255)
                                                                                                                                ggCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTg                     >  2:673122/1‑150 (MQ=255)
                                                                                                                                ggCCATGGTTTATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTg                     >  2:3931847/1‑150 (MQ=255)
                                                                                                                                 gCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgc                    <  1:725652/150‑1 (MQ=255)
                                                                                                                                  ccATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgcg                   >  1:1738433/1‑150 (MQ=255)
                                                                                                                                  ccATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgcg                   >  1:2856722/1‑150 (MQ=255)
                                                                                                                                  ccATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgcg                   >  2:4188484/1‑150 (MQ=255)
                                                                                                                                  ccATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTgcg                   <  1:1329239/150‑1 (MQ=255)
                                                                                                                                      ggTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGAt               >  2:2342009/1‑150 (MQ=255)
                                                                                                                                       gTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGAtt              <  2:1025048/150‑1 (MQ=255)
                                                                                                                                        tttATTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGAttt             >  1:3706257/1‑150 (MQ=255)
                                                                                                                                           aTTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCt          <  2:4139832/150‑1 (MQ=255)
                                                                                                                                           aTTCCGTTATAGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCt          >  2:5137906/1‑150 (MQ=255)
                                                                                                                                                gTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACg     <  1:1615216/150‑1 (MQ=255)
                                                                                                                                                gTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACg     <  1:3625535/150‑1 (MQ=255)
                                                                                                                                                gTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACg     >  2:2994786/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATGTCTGGAGACATCCTACAAACACCGGACGCACCAAAGCCACAGGGCGCGCTGGATAATTATTTTAAAATTACCGCTCGTGGCAGTACCGTTCGTCAGGAAGTACTGGCTGGCTTAACGACCTTTCTGGCCATGGTTTATTCCGTTATCGTCGTTCCGGGAATGCTGGGCAAAGCAGGTTTTCCTCCCGCAGCTGTGTTTGTTGCCACCTGTCTGGTCGCGGGCTTCGGCTCGTTGCTGATGGGATTATGGGCTAATTTGCCAATGGCGATTGGTTGCGCGATTTCCTTGACGGCG  >  CP009273/3020480‑3020776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑