Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,037,190 C→T 6.5% P57L (CCA→CTA)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,037,1900CT 6.5% 746.9 / 27.2 231P57L (CCA→CTA) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base C (102/114);  new base T (5/10);  total (107/124)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 239 total aligned reads displayed.

CTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAA  >  CP009273/3037041‑3037339
                                                                                                                                                     |                                                                                                                                                     
cTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGcc                                                                                                                                                       <  1:3105424/150‑1 (MQ=255)
          gATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATAc                                                                                                                                             <  2:4391025/150‑1 (MQ=255)
           aTTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACt                                                                                                                                            <  2:1520083/150‑1 (MQ=255)
              tCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATc                                                                                                                                         >  2:561635/1‑150 (MQ=255)
                 tATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCaa                                                                                                                                      >  1:1772490/1‑150 (MQ=255)
                    ggggCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAAcc                                                                                                                                   <  2:1443513/150‑1 (MQ=255)
                      ggcggcGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCAt                                                                                                                                 <  2:2520556/150‑1 (MQ=255)
                      ggcggcGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCAt                                                                                                                                 <  2:510966/150‑1 (MQ=255)
                       gcggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACTATCCAAACCATg                                                                                                                                <  2:3793107/150‑1 (MQ=255)
                       gcggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATg                                                                                                                                <  1:752020/150‑1 (MQ=255)
                        cggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGa                                                                                                                               >  1:4841637/1‑150 (MQ=255)
                         ggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGaa                                                                                                                              <  2:403258/150‑1 (MQ=255)
                         ggcgCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGaa                                                                                                                              >  2:2844766/1‑150 (MQ=255)
                              aGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGt                                                                                                                         <  2:3660016/150‑1 (MQ=255)
                              aGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGt                                                                                                                         <  1:3217752/150‑1 (MQ=255)
                               gTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGtt                                                                                                                        <  2:3726737/150‑1 (MQ=255)
                               gTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGtt                                                                                                                        <  2:694015/150‑1 (MQ=255)
                               gTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGtt                                                                                                                        >  1:1028049/1‑150 (MQ=255)
                                ttGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTg                                                                                                                       >  1:4992040/1‑150 (MQ=255)
                                ttGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTg                                                                                                                       >  1:4106312/1‑150 (MQ=255)
                                 tGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGa                                                                                                                      >  1:1980798/1‑150 (MQ=255)
                                    cGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGAttt                                                                                                                   <  1:4089430/150‑1 (MQ=255)
                                       tcatcaGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTa                                                                                                                <  2:921381/150‑1 (MQ=255)
                                         atcaGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATg                                                                                                              >  1:274300/1‑150 (MQ=255)
                                         atcaGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATg                                                                                                              >  1:519069/1‑150 (MQ=255)
                                           caGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGt                                                                                                            <  1:4585556/150‑1 (MQ=255)
                                            aGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTc                                                                                                           <  2:2252157/150‑1 (MQ=255)
                                             ggTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCa                                                                                                          <  2:3248086/150‑1 (MQ=255)
                                             ggTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCa                                                                                                          <  1:1558440/150‑1 (MQ=255)
                                              gTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCAc                                                                                                         <  2:3839671/150‑1 (MQ=255)
                                                 gAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtat                                                                                                      <  1:1073393/150‑1 (MQ=255)
                                                  aaGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGTGCGAAATCACCAAAGAAGTCTTGCCAGGACAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtata                                                                                                     >  2:1771375/1‑150 (MQ=255)
                                                  aaGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtata                                                                                                     >  2:2058826/1‑150 (MQ=255)
                                                  aaGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACtata                                                                                                     >  1:12874/1‑150 (MQ=255)
                                                   aGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATaa                                                                                                    >  2:2410323/1‑150 (MQ=255)
                                                   aGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATaa                                                                                                    <  2:2116957/150‑1 (MQ=255)
                                                      cggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGa                                                                                                 <  2:4713534/150‑1 (MQ=255)
                                                      cggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGa                                                                                                 >  2:3148274/1‑150 (MQ=255)
                                                      cggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGa                                                                                                 >  2:2422300/1‑150 (MQ=255)
                                                       ggcTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGaa                                                                                                >  2:4382750/1‑150 (MQ=255)
                                                          tGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGAc                                                                                             <  1:464354/150‑1 (MQ=255)
                                                             aaCAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATc                                                                                          >  1:406375/1‑150 (MQ=255)
                                                               cAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCaa                                                                                        <  2:2957008/150‑1 (MQ=255)
                                                                 aaGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGc                                                                                      >  2:3347288/1‑150 (MQ=255)
                                                                 aaGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGc                                                                                      <  1:3413179/150‑1 (MQ=255)
                                                                      ggAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTAttt                                                                                 <  1:2257087/150‑1 (MQ=255)
                                                                           aGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa                                                                            >  2:2107463/1‑150 (MQ=255)
                                                                           aGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa                                                                            >  2:683006/1‑150 (MQ=255)
                                                                            gggCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaa                                                                           >  2:4285802/1‑150 (MQ=255)
                                                                            gggCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaa                                                                           >  1:1185389/1‑150 (MQ=255)
                                                                            gggCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaa                                                                           <  2:4523298/150‑1 (MQ=255)
                                                                                cGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGATGATTTTTATGGTCACTATAAGGAAGACAACAAGCTATTTGCCGAAATgg                                                                       >  1:4865976/1‑150 (MQ=255)
                                                                                 gAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATggg                                                                      >  2:3416449/1‑150 (MQ=255)
                                                                                 gAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATggg                                                                      >  2:4825182/1‑150 (MQ=255)
                                                                                  aGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGc                                                                     <  2:4956056/150‑1 (MQ=255)
                                                                                   gCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCt                                                                    <  1:2651014/150‑1 (MQ=255)
                                                                                   gCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCt                                                                    <  2:704880/150‑1 (MQ=255)
                                                                                    cATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt                                                                   <  1:1057773/150‑1 (MQ=255)
                                                                                     aTTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTc                                                                  >  1:3607203/1‑150 (MQ=255)
                                                                                       ttGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCaa                                                                >  1:280748/1‑150 (MQ=255)
                                                                                         gtgACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt                                                              >  1:3857323/1‑150 (MQ=255)
                                                                                           gACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGt                                                            >  2:395822/1‑150 (MQ=255)
                                                                                            aCGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt                                                           <  1:2293553/150‑1 (MQ=255)
                                                                                             cGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGACAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTTTTTGCCGAAATGGGCTTCAAATGttt                                                          <  1:2693380/150‑1 (MQ=255)
                                                                                              gTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt                                                         >  2:3613205/1‑150 (MQ=255)
                                                                                                tCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg                                                       <  1:4255621/150‑1 (MQ=255)
                                                                                                 cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGt                                                      <  2:4189028/150‑1 (MQ=255)
                                                                                                   gACCGGTGGCGCACACGGCTGGCCGCGCGAAATCACCAAAGAAGTCTTTCCAGTAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTAc                                                    <  2:4349968/150‑1 (MQ=255)
                                                                                                   gACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTAc                                                    >  2:1243099/1‑150 (MQ=255)
                                                                                                       ggTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATcc                                                <  1:1390888/150‑1 (MQ=255)
                                                                                                       ggTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATcc                                                <  1:2017291/150‑1 (MQ=255)
                                                                                                       ggTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATcc                                                <  1:1551642/150‑1 (MQ=255)
                                                                                                         tGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt                                              >  2:42541/1‑150 (MQ=255)
                                                                                                          ggCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAtt                                             <  1:4532005/150‑1 (MQ=255)
                                                                                                           gcgcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTg                                            <  1:3148274/150‑1 (MQ=255)
                                                                                                             gcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGcc                                          >  2:4884711/1‑150 (MQ=255)
                                                                                                              cacaCGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCt                                         >  1:3945587/1‑150 (MQ=255)
                                                                                                                  cGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAc                                     >  2:35559/1‑150 (MQ=255)
                                                                                                                   ggCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcg                                    <  2:1884098/150‑1 (MQ=255)
                                                                                                                    gCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc                                   >  2:616423/1‑150 (MQ=255)
                                                                                                                      gTGCCGCGCGAAATCACCAAAGAAGTCTTGCTAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc                                 >  2:4683035/1‑150 (MQ=255)
                                                                                                                       tGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCa                                >  2:30061/1‑150 (MQ=255)
                                                                                                                          cgcgcgAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                             >  1:2116887/1‑150 (MQ=255)
                                                                                                                          cgcgcgAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                             >  1:2117025/1‑150 (MQ=255)
                                                                                                                          cgcgcgAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                             <  1:3376793/150‑1 (MQ=255)
                                                                                                                            cgcgAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt                           >  2:779607/1‑150 (MQ=255)
                                                                                                                              cgAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc                         <  1:4696799/150‑1 (MQ=255)
                                                                                                                                aaaTCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                       >  2:5168099/1‑150 (MQ=255)
                                                                                                                                  aTCACCACAGAAGTCTGGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa                     <  2:4129956/150‑1 (MQ=255)
                                                                                                                                      ccAACGACGTCTTGCCAGCAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGTACTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg                 <  1:4631334/150‑1 (MQ=255)
                                                                                                                                       cAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa                >  2:2214675/1‑150 (MQ=255)
                                                                                                                                           gaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGaa            >  1:301825/1‑150 (MQ=255)
                                                                                                                                           gaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGaa            <  1:968965/150‑1 (MQ=255)
                                                                                                                                           gaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGaa            <  1:2352779/150‑1 (MQ=255)
                                                                                                                                            aagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg           >  2:1987164/1‑150 (MQ=255)
                                                                                                                                                cTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCa       <  2:2109963/150‑1 (MQ=255)
                                                                                                                                                    ccAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGACGCTCAGCCa   >  1:3486578/1‑150 (MQ=255)
                                                                                                                                                    ccAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa   <  2:3304794/150‑1 (MQ=255)
                                                                                                                                                     cAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa  >  1:926993/1‑150 (MQ=255)
                                                                                                                                                     cAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa  >  1:3753598/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAA  >  CP009273/3037041‑3037339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑