Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,422,390 C→A 8.9% intergenic (‑269/‑205) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,422,3900CA 8.9% 803.8 / 43.9 235intergenic (‑269/‑205)rrsD/yrdA16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
Reads supporting (aligned to +/- strand):  ref base C (98/116);  new base A (12/9);  total (110/125)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 237 total aligned reads displayed.

TTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACTTGCA  >  CP009273/3422241‑3422539
                                                                                                                                                     |                                                                                                                                                     
ttGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCATGATTTTTTTCTCTTCGTTGTTACGACCATCCTGTGAAGTGTTTc                                                                                                                                                       >  2:2641487/1‑150 (MQ=255)
  gTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTAGTTGTTCCGACCATCCTGTGAAGTGTTTCAc                                                                                                                                                     >  2:1820774/1‑150 (MQ=255)
     tGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGtt                                                                                                                                                  <  2:1138736/150‑1 (MQ=255)
        tAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTgtc                                                                                                                                               <  1:1784468/150‑1 (MQ=255)
        tAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTgtc                                                                                                                                               <  2:1003323/150‑1 (MQ=255)
           aTTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTgtcgtc                                                                                                                                            <  1:4982754/150‑1 (MQ=255)
             tGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGtctc                                                                                                                                          >  1:1892666/1‑150 (MQ=255)
                 aaaGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACg                                                                                                                                      <  1:1052439/150‑1 (MQ=255)
                 aaaGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACg                                                                                                                                      >  1:4243636/1‑150 (MQ=255)
                 aaaGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTAACGTTGTCGTCTCAACg                                                                                                                                      >  2:5007424/1‑150 (MQ=255)
                  aaGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACgg                                                                                                                                     >  2:2140220/1‑150 (MQ=255)
                   agagCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGa                                                                                                                                    <  2:4621338/150‑1 (MQ=255)
                     agCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAgg                                                                                                                                  >  1:1760740/1‑150 (MQ=255)
                      gCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTAAACGGAGgc                                                                                                                                 >  1:4628211/1‑150 (MQ=255)
                        aGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGgcgc                                                                                                                               >  1:2500213/1‑150 (MQ=255)
                        aGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGgcgc                                                                                                                               >  1:2340482/1‑150 (MQ=255)
                         gTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCa                                                                                                                              >  2:445976/1‑150 (MQ=255)
                           tGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCAtt                                                                                                                            <  2:3677972/150‑1 (MQ=255)
                            gCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATta                                                                                                                           <  1:4588785/150‑1 (MQ=255)
                             cGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATtat                                                                                                                          >  2:2526251/1‑150 (MQ=255)
                             cGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATtat                                                                                                                          <  2:692053/150‑1 (MQ=255)
                              gACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATtata                                                                                                                         <  2:3901205/150‑1 (MQ=255)
                              gACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATtata                                                                                                                         >  2:4352015/1‑150 (MQ=255)
                                cgcgGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAgg                                                                                                                       <  2:2919422/150‑1 (MQ=255)
                                    gCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATc                                                                                                                   <  2:1771280/150‑1 (MQ=255)
                                      tttCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATccc                                                                                                                 >  2:1076311/1‑150 (MQ=255)
                                      tttCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTAACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATccc                                                                                                                 >  1:711587/1‑150 (MQ=255)
                                          aGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAAtt                                                                                                             <  1:4825843/150‑1 (MQ=255)
                                          aGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAAtt                                                                                                             <  1:1331216/150‑1 (MQ=255)
                                           gCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAAttt                                                                                                            <  2:1418281/150‑1 (MQ=255)
                                             tCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAAttttt                                                                                                          <  2:4769883/150‑1 (MQ=255)
                                             tCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAAttttt                                                                                                          <  1:255744/150‑1 (MQ=255)
                                               aCTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTg                                                                                                        <  2:4841762/150‑1 (MQ=255)
                                                 tGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGca                                                                                                      <  1:2306127/150‑1 (MQ=255)
                                                    cgcgAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTAACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACaa                                                                                                   >  2:3630015/1‑150 (MQ=255)
                                                        aGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTAt                                                                                               >  1:1260845/1‑150 (MQ=255)
                                                         ggTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTAtt                                                                                              <  2:894651/150‑1 (MQ=255)
                                                           tGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTAtttt                                                                                            <  2:983273/150‑1 (MQ=255)
                                                                tataTTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGa                                                                                       >  1:449986/1‑150 (MQ=255)
                                                                tataTTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGa                                                                                       >  2:2810186/1‑150 (MQ=255)
                                                                 atatTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGAt                                                                                      <  2:4758664/150‑1 (MQ=255)
                                                                   atTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCt                                                                                    >  1:3314325/1‑150 (MQ=255)
                                                                    ttACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCtt                                                                                   >  1:5088557/1‑150 (MQ=255)
                                                                    ttACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCtt                                                                                   <  1:2209522/150‑1 (MQ=255)
                                                                      aCGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCtttt                                                                                 <  2:437344/150‑1 (MQ=255)
                                                                          tttCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTc                                                                             <  1:5023131/150‑1 (MQ=255)
                                                                           ttCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCt                                                                            <  1:179083/150‑1 (MQ=255)
                                                                             ccTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCtgt                                                                          <  2:4613265/150‑1 (MQ=255)
                                                                              ctctTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCtgtt                                                                         <  2:1688177/150‑1 (MQ=255)
                                                                                ctTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCtttttg                                                                       >  2:1562597/1‑150 (MQ=255)
                                                                                  ttCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCtgtttgtt                                                                     <  1:3623171/150‑1 (MQ=255)
                                                                                     agagTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTgttgtt                                                                  >  1:2230401/1‑150 (MQ=255)
                                                                                      gagTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGttt                                                                 <  2:217336/150‑1 (MQ=255)
                                                                                      gagTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGttt                                                                 <  2:23286/150‑1 (MQ=255)
                                                                                       agTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGtttt                                                                <  2:2116653/150‑1 (MQ=255)
                                                                                       agTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGGTTGTTGtttt                                                                >  2:962460/1‑150 (MQ=255)
                                                                                         tCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGtttttt                                                              <  1:4564693/150‑1 (MQ=255)
                                                                                          cAACCCTGAATTTCAGGATTTTTTTCTCTACGTTGTTCCGACCCTCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTc                                                             >  2:4304040/1‑150 (MQ=255)
                                                                                               cTGAATTTCAGGATTTTTTTCTCTTCTTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTCTAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCt                                                        >  2:4896374/1‑150 (MQ=255)
                                                                                                 gAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCttt                                                      <  2:4120935/150‑1 (MQ=255)
                                                                                                  aaTTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCtttt                                                     >  1:187079/1‑150 (MQ=255)
                                                                                                  aaTTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCtttt                                                     <  1:1687031/150‑1 (MQ=255)
                                                                                                   aTTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCttttt                                                    >  1:5149094/1‑150 (MQ=255)
                                                                                                    tttCAGGATTTTTTTCTCTTCGTTGTTCAGAACATCCTGTGAAGTGTTTCACGTTGTAGTCTCAACGGAGGAGCATTATATGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTtg                                                   <  1:2706027/150‑1 (MQ=255)
                                                                                                      tCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTtgct                                                 <  2:1590601/150‑1 (MQ=255)
                                                                                                      tCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAAGTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTtgct                                                 >  2:3323966/1‑150 (MQ=255)
                                                                                                       cAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTAACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTtgctg                                                >  2:2915268/1‑150 (MQ=255)
                                                                                                        aGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTtgctgc                                               >  2:2264015/1‑150 (MQ=255)
                                                                                                           aTTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGAAAt                                            >  1:736870/1‑150 (MQ=255)
                                                                                                             ttttttCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCg                                          <  1:841278/150‑1 (MQ=255)
                                                                                                              tttttCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGc                                         >  1:2458935/1‑150 (MQ=255)
                                                                                                               ttttCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGca                                        <  2:1732577/150‑1 (MQ=255)
                                                                                                                tttCTCTTCGTTGGTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGcac                                       <  2:2844644/150‑1 (MQ=255)
                                                                                                                  tctcTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGcacac                                     >  1:1327163/1‑150 (MQ=255)
                                                                                                                    tctTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACaa                                   <  2:2230401/150‑1 (MQ=255)
                                                                                                                     ctTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTGTTTGCTGCAATCGCACACaaa                                  >  2:2450380/1‑150 (MQ=255)
                                                                                                                       tCGTTGTTCCGACCATCCTGTGAGGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAAc                                <  1:1403442/150‑1 (MQ=255)
                                                                                                                          ttgttCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGt                             <  1:1561499/150‑1 (MQ=255)
                                                                                                                                cGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCtttt                       >  1:2312223/1‑150 (MQ=255)
                                                                                                                                cGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCtttt                       >  1:3429943/1‑150 (MQ=255)
                                                                                                                                 gACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCttttt                      <  2:4041104/150‑1 (MQ=255)
                                                                                                                                   ccATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTg                    >  1:3713124/1‑150 (MQ=255)
                                                                                                                                    cATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGc                   >  2:588935/1‑150 (MQ=255)
                                                                                                                                    cATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGc                   >  2:1109874/1‑150 (MQ=255)
                                                                                                                                    cATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGc                   <  1:3553674/150‑1 (MQ=255)
                                                                                                                                      tCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCAt                 >  2:1888781/1‑150 (MQ=255)
                                                                                                                                      tCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCAt                 <  1:1512336/150‑1 (MQ=255)
                                                                                                                                      tCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCAt                 >  2:1750295/1‑150 (MQ=255)
                                                                                                                                        cTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACTGAGCTTTTTTGCATAc               >  1:3152684/1‑150 (MQ=255)
                                                                                                                                           tgAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTaa            <  1:3044664/150‑1 (MQ=255)
                                                                                                                                            gAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTaaa           >  1:4273920/1‑150 (MQ=255)
                                                                                                                                            gAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTaaa           >  1:1123824/1‑150 (MQ=255)
                                                                                                                                            gAAGTGTTTAACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTaaa           >  1:3854656/1‑150 (MQ=255)
                                                                                                                                                 gtTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGaat      <  1:941667/150‑3 (MQ=255)
                                                                                                                                                 gtTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACt      <  1:4893766/150‑1 (MQ=255)
                                                                                                                                                 gtTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACt      <  1:102926/150‑1 (MQ=255)
                                                                                                                                                  tttCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACtt     >  1:1969964/1‑150 (MQ=255)
                                                                                                                                                  tttAACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACtt     >  1:4389428/1‑150 (MQ=255)
                                                                                                                                                   ttCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACTTg    <  1:1466015/150‑1 (MQ=255)
                                                                                                                                                     cACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACTTGca  >  1:813911/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGATCTTTTTTTCTGTTTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAAACGGTGCTTTTTTGCATACTAAAAGACTTGCA  >  CP009273/3422241‑3422539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑