Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,543,959 G→T 90.4% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,543,9590GT90.4% 655.9 / 59.2 235A625E (GCG→GAG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (12/11);  new base T (117/95);  total (129/106)
Fisher's exact test for biased strand distribution p-value = 8.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 242 total aligned reads displayed.

AGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCG  >  CP009273/3543810‑3544103
                                                                                                                                                     |                                                                                                                                                
  atatTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                                <  1:4859175/150‑1 (MQ=255)
  atatTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                                <  1:3753744/150‑1 (MQ=255)
    atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCgg                                                                                                                                              >  2:3896255/1‑150 (MQ=255)
      tttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                            >  1:4602385/1‑150 (MQ=255)
      tttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGa                                                                                                                                            >  1:637240/1‑150 (MQ=255)
        tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                          <  2:1109406/150‑1 (MQ=255)
          ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAg                                                                                                                                        >  1:2561176/1‑150 (MQ=255)
           tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGt                                                                                                                                       >  1:1158502/1‑150 (MQ=255)
           tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGt                                                                                                                                       >  1:4549111/1‑150 (MQ=255)
           tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGt                                                                                                                                       <  2:4630487/150‑1 (MQ=255)
            cACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                      >  2:3223697/1‑150 (MQ=255)
            cACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                      >  2:3223602/1‑150 (MQ=255)
            cACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                      >  1:3502763/1‑150 (MQ=255)
            cACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGGTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtt                                                                                                                                      >  2:4486548/1‑150 (MQ=255)
             aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttt                                                                                                                                     >  1:2505829/1‑150 (MQ=255)
             aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttt                                                                                                                                     >  1:2240657/1‑150 (MQ=255)
                    ttCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgcc                                                                                                                              >  2:827258/1‑150 (MQ=255)
                    ttCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTGTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgcc                                                                                                                              <  2:2497749/150‑1 (MQ=255)
                       tcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgccgcc                                                                                                                           >  1:1250740/1‑150 (MQ=255)
                        cGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgccgccg                                                                                                                          >  1:3699538/1‑150 (MQ=255)
                         ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGa                                                                                                                         <  1:2850704/150‑1 (MQ=255)
                         ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGa                                                                                                                         >  1:4082929/1‑150 (MQ=255)
                            gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                      >  1:3238503/1‑150 (MQ=255)
                            gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                      >  1:1830818/1‑150 (MQ=255)
                            gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                      <  1:340191/150‑1 (MQ=255)
                                  aaCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaata                                                                                                                <  1:1787255/150‑1 (MQ=255)
                                    cGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaataat                                                                                                              >  1:711219/1‑150 (MQ=255)
                                     gTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATc                                                                                                             >  2:2978218/1‑150 (MQ=255)
                                      ttCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATcc                                                                                                            <  1:3256782/150‑1 (MQ=255)
                                      ttCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATcc                                                                                                            >  1:3113405/1‑150 (MQ=255)
                                        cGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCgg                                                                                                          <  2:1603561/150‑1 (MQ=255)
                                          ccccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCa                                                                                                        >  2:2754427/1‑150 (MQ=255)
                                          ccccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCa                                                                                                        >  2:2525768/1‑150 (MQ=255)
                                               tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAac                                                                                                   <  1:2248625/150‑1 (MQ=255)
                                               tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAac                                                                                                   <  1:4507483/150‑1 (MQ=255)
                                                ccAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAaca                                                                                                  >  1:5077084/1‑150 (MQ=255)
                                                  aGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcacc                                                                                                >  1:330843/1‑150 (MQ=255)
                                                     gTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACaccacc                                                                                             >  2:2311564/1‑150 (MQ=255)
                                                      tACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCt                                                                                            >  2:5120669/1‑150 (MQ=255)
                                                       aCCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCtt                                                                                           <  2:3846761/150‑1 (MQ=255)
                                                          gACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAACCGGCAGGAACACCACCTTCaa                                                                                        >  1:2321440/1‑150 (MQ=255)
                                                            cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACt                                                                                      >  2:2936550/1‑150 (MQ=255)
                                                             aGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACtt                                                                                     >  1:4646426/1‑150 (MQ=255)
                                                               tAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACCCCACCTTCAACTTAt                                                                                   >  2:3622168/1‑150 (MQ=255)
                                                                 aGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCg                                                                                 <  2:4326141/150‑1 (MQ=255)
                                                                   cgcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTATTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTACGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGcc                                                                               >  1:1104969/1‑150 (MQ=255)
                                                                    gcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCa                                                                              <  2:1023076/150‑1 (MQ=255)
                                                                      aCCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAAc                                                                            >  1:4185503/1‑150 (MQ=255)
                                                                       ccATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAAcc                                                                           >  1:1963188/1‑150 (MQ=255)
                                                                           tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCg                                                                       >  2:4201497/1‑150 (MQ=255)
                                                                             aGCGCCAGTTTCATAGTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGa                                                                     <  1:1018414/150‑1 (MQ=255)
                                                                              gcgcCAGTTTCATATTGCCGGTACCGGATGCTTCTTTACCTTCAGTCGAAATTTTTTCGGTGATATCCGCCTCCTGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTAATCTTATCGCCAACCAGCGGAt                                                                    <  1:4060448/150‑1 (MQ=255)
                                                                               cgcCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATc                                                                   <  1:1448866/150‑1 (MQ=255)
                                                                                  cAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgtt                                                                >  1:1719565/1‑150 (MQ=255)
                                                                                  cAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgtt                                                                <  2:2895802/150‑1 (MQ=255)
                                                                                   aGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttg                                                               >  2:62023/1‑150 (MQ=255)
                                                                                   aGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttg                                                               >  2:1925657/1‑150 (MQ=255)
                                                                                     tttCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttgtt                                                             <  2:3331225/150‑1 (MQ=255)
                                                                                        cATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAt                                                          <  2:3045875/150‑1 (MQ=255)
                                                                                         atatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACGTATCGCCAACCAGCGGATCGTTGTTGATc                                                         >  2:570556/1‑150 (MQ=255)
                                                                                           atTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAc                                                       >  2:3304601/1‑150 (MQ=255)
                                                                                           atTGCCGGTACCGGAAGCTTCTGTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAc                                                       <  2:2061170/150‑1 (MQ=255)
                                                                                                  gTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGcc                                                >  1:4592092/1‑150 (MQ=255)
                                                                                                     ccGGTAGCTTCTTTACCTGCAGTCGAATGTTGTTCGGAGATATCCGCCGCCGTGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTCTCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACt                                             <  1:3724213/150‑1 (MQ=255)
                                                                                                        gAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttg                                          <  1:2012077/150‑1 (MQ=255)
                                                                                                         aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCTGATCGTTGTTGATCACGTCAGCCACTttgt                                         <  2:886262/150‑1 (MQ=255)
                                                                                                          aGCTTCTTTACCTGCAGTCGAAATTTGTTCGGATATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAAAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgtt                                        >  1:3853143/1‑150 (MQ=255)
                                                                                                          aGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgtt                                        <  2:2240657/150‑1 (MQ=255)
                                                                                                          aGCGTCTGTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgtt                                        <  2:4878248/150‑1 (MQ=255)
                                                                                                             ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGAt                                     >  2:3160746/1‑150 (MQ=255)
                                                                                                             ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGAt                                     <  2:3788345/150‑1 (MQ=255)
                                                                                                              tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATc                                    >  2:206686/1‑150 (MQ=255)
                                                                                                              tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATc                                    >  2:3156816/1‑150 (MQ=255)
                                                                                                              tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATc                                    <  1:4314906/150‑1 (MQ=255)
                                                                                                                     cTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGa                             >  1:2918890/1‑150 (MQ=255)
                                                                                                                       gCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGata                           <  2:2135026/150‑1 (MQ=255)
                                                                                                                        cAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataa                          >  1:5095325/1‑150 (MQ=255)
                                                                                                                         aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                         <  1:1594945/150‑1 (MQ=255)
                                                                                                                         aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                         >  2:4328658/1‑150 (MQ=255)
                                                                                                                         aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                         >  2:4010340/1‑150 (MQ=255)
                                                                                                                         aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                         >  2:1636030/1‑150 (MQ=255)
                                                                                                                         aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                         >  2:1456331/1‑150 (MQ=255)
                                                                                                                           tCGAAATTTGGTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATACTCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAatat                       <  2:4280373/150‑1 (MQ=255)
                                                                                                                            cGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                      >  1:4695499/1‑150 (MQ=255)
                                                                                                                             gAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttc                     <  1:3621455/150‑1 (MQ=255)
                                                                                                                                aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                  <  2:243112/150‑1 (MQ=255)
                                                                                                                                 tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATACTCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCg                 <  2:4788390/150‑1 (MQ=255)
                                                                                                                                 tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCg                 <  2:4283436/150‑1 (MQ=255)
                                                                                                                                 tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCg                 >  1:2181368/1‑150 (MQ=255)
                                                                                                                                   tGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGcc               <  1:3106255/150‑1 (MQ=255)
                                                                                                                                      tCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAgg            >  1:2409435/1‑150 (MQ=255)
                                                                                                                                        ggAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta          >  1:2628282/1‑150 (MQ=255)
                                                                                                                                         gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgaag         <  2:3932973/150‑1 (MQ=255)
                                                                                                                                         gagaTATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag         <  1:831254/150‑1 (MQ=255)
                                                                                                                                          agaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagt        >  1:4236420/1‑150 (MQ=255)
                                                                                                                                           gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagta       <  2:1250740/150‑1 (MQ=255)
                                                                                                                                            atatCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGtagtag      <  2:1727734/150‑1 (MQ=255)
                                                                                                                                             tatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc     >  1:1874812/1‑150 (MQ=255)
                                                                                                                                             tatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc     >  2:3582671/1‑150 (MQ=255)
                                                                                                                                               tCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGccc   <  1:979670/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCG  >  CP009273/3543810‑3544103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑