Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,717,788 C→A 71.5% P297P (CCG→CCT)  glyQ ← glycine tRNA synthetase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,717,7880CA71.5% 394.4 / 255.5 263P297P (CCG→CCT) glyQglycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (41/34);  new base A (97/91);  total (138/125)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 263 total aligned reads displayed.

GGCCAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAA  >  CP009273/3717640‑3717936
                                                                                                                                                    |                                                                                                                                                    
ggCCAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCg                                                                                                                                                     <  1:3542928/150‑1 (MQ=255)
     gCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAg                                                                                                                                                >  1:2426077/1‑150 (MQ=255)
       gTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGcc                                                                                                                                              >  1:838092/1‑150 (MQ=255)
                 tCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCa                                                                                                                                    <  1:288427/150‑1 (MQ=255)
                  ccGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCAc                                                                                                                                   <  1:2130862/150‑1 (MQ=255)
                      aGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGa                                                                                                                               >  2:2564226/1‑150 (MQ=255)
                         aaaGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGACGCCGAGGGCTTCACGGGAAGc                                                                                                                            >  2:3396574/1‑150 (MQ=255)
                         aaaGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGc                                                                                                                            >  1:4885376/1‑150 (MQ=255)
                              tCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCgtagt                                                                                                                       >  1:2481486/1‑150 (MQ=255)
                               cGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgtagta                                                                                                                      <  2:3068758/150‑1 (MQ=255)
                                gcagcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTAt                                                                                                                     <  1:2783172/150‑1 (MQ=255)
                                gcagcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTAt                                                                                                                     >  1:322394/1‑150 (MQ=255)
                                 cagcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATg                                                                                                                    >  2:4539727/1‑150 (MQ=255)
                                  agcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGc                                                                                                                   <  2:252718/150‑1 (MQ=255)
                                  agcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGc                                                                                                                   >  2:592443/1‑150 (MQ=255)
                                  agcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGc                                                                                                                   <  2:3942819/150‑1 (MQ=255)
                                   gcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCt                                                                                                                  <  1:3324520/150‑1 (MQ=255)
                                    caAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                                 <  1:4336799/150‑1 (MQ=255)
                                     aaaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTc                                                                                                                >  1:4804191/1‑150 (MQ=255)
                                      aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCt                                                                                                               >  2:831467/1‑150 (MQ=255)
                                          aCTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCa                                                                                                           <  1:1911514/150‑1 (MQ=255)
                                            tCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACt                                                                                                         <  1:1954835/150‑1 (MQ=255)
                                            tCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACt                                                                                                         <  1:4025486/150‑1 (MQ=255)
                                             cAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTg                                                                                                        >  1:3098404/1‑150 (MQ=255)
                                             cAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTg                                                                                                        <  2:2737982/150‑1 (MQ=255)
                                              aGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGc                                                                                                       >  2:4652000/1‑150 (MQ=255)
                                               gCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCt                                                                                                      <  1:2977422/150‑1 (MQ=255)
                                                ccAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCtt                                                                                                     <  1:4314284/150‑1 (MQ=255)
                                                 cAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCttt                                                                                                    >  1:2268127/1‑150 (MQ=255)
                                                 cAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCttt                                                                                                    <  2:38258/150‑1 (MQ=255)
                                                 cAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCttt                                                                                                    >  1:2268145/1‑150 (MQ=255)
                                                      tGCGCAGTGCTTTTGGTGGCAGCTCTTCAGGGCCGATTTCCACCAGAAAAGGTTTCTCAGACATAGCCGCCTCTTACGTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCa                                                                                               <  2:3672938/150‑1 (MQ=255)
                                                       gcgcAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAg                                                                                              <  2:1636368/150‑1 (MQ=255)
                                                           aGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTg                                                                                          >  2:1900004/1‑150 (MQ=255)
                                                           aGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTg                                                                                          >  2:3862622/1‑150 (MQ=255)
                                                              gCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                       >  2:1724087/1‑150 (MQ=255)
                                                                ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAAt                                                                                     <  2:2376276/150‑1 (MQ=255)
                                                                ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAAt                                                                                     <  2:2033478/150‑1 (MQ=255)
                                                                ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAAt                                                                                     >  1:525413/1‑150 (MQ=255)
                                                                ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTGTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAAt                                                                                     <  2:722097/150‑1 (MQ=255)
                                                                 tttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATg                                                                                    >  2:4819812/1‑150 (MQ=255)
                                                                 tttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATg                                                                                    >  1:383036/1‑150 (MQ=255)
                                                                  ttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATgc                                                                                   <  1:1448015/150‑1 (MQ=255)
                                                                      tggCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAg                                                                               <  1:1584149/150‑1 (MQ=255)
                                                                      tggCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAg                                                                               >  2:2754257/1‑150 (MQ=255)
                                                                          aGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                           >  2:1340047/1‑150 (MQ=255)
                                                                            ctctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTa                                                                         <  2:183059/150‑1 (MQ=255)
                                                                            ctctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTa                                                                         >  1:2111451/1‑150 (MQ=255)
                                                                               ttCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgcg                                                                      <  2:2956878/150‑1 (MQ=255)
                                                                                 cAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCt                                                                    <  2:1579893/150‑1 (MQ=255)
                                                                                  aGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGACTGCGCAGAATGTAGCGCTg                                                                   >  2:2324715/1‑150 (MQ=255)
                                                                                  aGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTg                                                                   >  1:2185964/1‑150 (MQ=255)
                                                                                   gTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGa                                                                  >  1:772942/1‑150 (MQ=255)
                                                                                    tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGAc                                                                 <  1:1755417/150‑1 (MQ=255)
                                                                                    tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGAc                                                                 <  2:1337539/150‑1 (MQ=255)
                                                                                    tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGAc                                                                 <  2:4977469/150‑1 (MQ=255)
                                                                                        gATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTc                                                             <  2:4636590/150‑1 (MQ=255)
                                                                                         aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCg                                                            >  1:3446345/1‑150 (MQ=255)
                                                                                         aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGAGTAGTATGCTTCTGCCACTGCTTTGTACAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTct                                                            >  2:1762988/1‑149 (MQ=255)
                                                                                              caccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGAc                                                       <  2:1897609/150‑1 (MQ=255)
                                                                                               accaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGAc                                                       >  1:4530432/1‑149 (MQ=255)
                                                                                                 caGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGga                                                    >  2:4314672/1‑150 (MQ=255)
                                                                                                    aaaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGAt                                                 >  1:5123182/1‑150 (MQ=255)
                                                                                                     aaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATg                                                >  1:4420496/1‑150 (MQ=255)
                                                                                                     aaaGTGTTCTCAGACATAGCCGCCTCTTACTGATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGGAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATg                                                <  2:3560214/150‑1 (MQ=255)
                                                                                                       aGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGc                                              <  1:3374328/150‑1 (MQ=255)
                                                                                                         ttttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCtt                                            <  2:554012/150‑1 (MQ=255)
                                                                                                           ttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTa                                          >  1:2049136/1‑150 (MQ=255)
                                                                                                             ctcAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcg                                        >  2:4052521/1‑150 (MQ=255)
                                                                                                               cAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcgcg                                      >  2:3278098/1‑150 (MQ=255)
                                                                                                               cAGACATAGCCGCCTCTGACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcgcg                                      <  2:1543604/150‑1 (MQ=255)
                                                                                                                 gACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCa                                    <  2:236716/150‑1 (MQ=255)
                                                                                                                 gACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCa                                    <  2:3088136/150‑1 (MQ=255)
                                                                                                                 gACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCa                                    >  1:842174/1‑150 (MQ=255)
                                                                                                                   cATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATc                                  >  1:1251450/1‑150 (MQ=255)
                                                                                                                   cATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATc                                  >  1:4100287/1‑150 (MQ=255)
                                                                                                                   cATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATc                                  <  1:3776013/150‑1 (MQ=255)
                                                                                                                    aTAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATcc                                 <  1:1556354/150‑1 (MQ=255)
                                                                                                                     tAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCca                                >  1:967768/1‑150 (MQ=255)
                                                                                                                     tAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCca                                >  1:3696999/1‑150 (MQ=255)
                                                                                                                        ccgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                             >  1:3353852/1‑150 (MQ=255)
                                                                                                                         cgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagcag                            >  2:4462677/1‑150 (MQ=255)
                                                                                                                          gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAgg                           >  2:4541738/1‑150 (MQ=255)
                                                                                                                             tctTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTg                        <  2:48911/150‑1 (MQ=255)
                                                                                                                             tctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTg                        >  1:3601224/1‑150 (MQ=255)
                                                                                                                              cttacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTACACGGGAAGCGTAGTATGCTACTGCCACTGCTTAGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGATGa                       >  2:2585952/1‑150 (MQ=255)
                                                                                                                               ttacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                      <  2:2857416/150‑1 (MQ=255)
                                                                                                                               ttactgaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                      <  2:2857399/150‑1 (MQ=255)
                                                                                                                                tacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAg                     <  2:4392960/150‑1 (MQ=255)
                                                                                                                                tacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAg                     >  2:4279062/1‑150 (MQ=255)
                                                                                                                                  cttaTCTTTGTTGCACATAGGGAAGCCGTGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCt                   >  2:2172294/1‑150 (MQ=255)
                                                                                                                                  cttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCt                   >  2:254952/1‑150 (MQ=255)
                                                                                                                                     aTCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCtgtg                <  1:4550351/150‑1 (MQ=255)
                                                                                                                                         ttgttgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcg            <  1:3794243/150‑1 (MQ=255)
                                                                                                                                             tgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCtt        >  1:1800282/1‑150 (MQ=255)
                                                                                                                                             tgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCtt        >  1:1000961/1‑150 (MQ=255)
                                                                                                                                              gCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCttt       <  1:3915717/150‑1 (MQ=255)
                                                                                                                                               cacaTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTc      >  1:2326092/1‑150 (MQ=255)
                                                                                                                                                 caTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAg    <  1:3862622/150‑1 (MQ=255)
                                                                                                                                                 caTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAg    <  1:4644806/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCCAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAA  >  CP009273/3717640‑3717936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑