BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,721,485	T→G	9.5%	Y416S (TAC→TCC)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,721,485	0	T	G	9.5%	398.8 / 9.9	174	Y416S (TAC→TCC)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base T (50/104); new base G (18/2); total (68/106)
Fisher's exact test for biased strand distribution p-value = 8.72e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721336‑3721634

gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                       <  2:4432682/150‑1 (MQ=255)

gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                       <  2:3500834/150‑1 (MQ=255)

    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCTGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                   <  2:1433579/150‑1 (MQ=255)

    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                   <  1:3368602/150‑1 (MQ=255)

          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGGACTCGAgctg                                                                                                                                             >  2:4101230/1‑150 (MQ=255)

            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                           <  2:4782528/150‑1 (MQ=255)

            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                           <  2:3218992/150‑1 (MQ=255)

            aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                           <  1:2224580/150‑1 (MQ=255)

              cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCGCGTACGGTCGTGGAGCTGCTGa                                                                                                                                         >  1:2423486/1‑150 (MQ=255)

                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                                    <  2:2717070/150‑1 (MQ=255)

                     cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGCCGGTAGTCGTGCTGCTGACCgggga                                                                                                                                  >  2:3186205/1‑146 (MQ=255)

                     cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                                  <  2:1440155/150‑1 (MQ=255)

                       tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGGCAGCGCGCTGCTGACCGcgggtc                                                                                                                                >  2:4713642/1‑145 (MQ=255)

                         tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGTCCGGGAATCGGGCTGCTGGCCGCTgggatc                                                                                                                              >  2:3320704/1‑145 (MQ=255)

                         tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATAGAGCTGCTGACCGCTGATATc                                                                                                                              <  2:175256/150‑1 (MQ=255)

                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                             <  2:3858013/150‑1 (MQ=255)

                           gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                                                            <  1:4148121/150‑1 (MQ=255)

                           gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                                                            <  1:773131/150‑1 (MQ=255)

                            tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTGCGGTAGGCGAGCTGGTGGGCGCGGAGGTCCGc                                                                                                                           >  2:1890554/1‑150 (MQ=255)

                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                   <  2:3154740/150‑1 (MQ=255)

                                      tGTTCTAACGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                 <  1:1574922/150‑1 (MQ=255)

                                       gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGGACTCGTGCGGCTGACCGCTGATCTCCGCCCGCGTCGGAc                                                                                                                >  2:4410713/1‑150 (MQ=255)

                                       gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                                <  1:4913488/150‑1 (MQ=255)

                                        ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGANg                                                                                                               <  1:2740272/150‑1 (MQ=255)

                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGGGTCGTGCTGGTGTCCGCGGGTGTGCGCCAGGCTCTGGCgg                                                                                                              >  1:679912/1‑149 (MQ=255)

                                          cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                             <  1:4418245/150‑1 (MQ=255)

                                           tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                            <  1:472553/150‑1 (MQ=255)

                                           tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                            <  2:858263/150‑1 (MQ=255)

                                             aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                                                          <  2:4672031/150‑1 (MQ=255)

                                              cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCGTCCCCCCCCGTACGGGAGTCGGGCTGCGGTCCGCGGGGCTCCGCCAGCTTCGGGCGCCCGtc                                                                                                         >  1:3466978/1‑149 (MQ=255)

                                              cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTACCCCCCCGGGCGGTAATCGGGCTGCTGTCCGCTGGGAGCCGCCCGCATCGGACGCCCGtc                                                                                                         >  1:862795/1‑149 (MQ=255)

                                                   gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                    <  2:4335935/150‑1 (MQ=255)

                                                   gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                    <  2:2446743/150‑1 (MQ=255)

                                                     tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                                  <  1:2110728/150‑1 (MQ=255)

                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                                 <  1:3464165/150‑1 (MQ=255)

                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                                 <  1:861278/150‑1 (MQ=255)

                                                         gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGTGGTGCTGACCGGTGATATCCGCCAGCATCTGTCGCCAGTACTCACTAcgcg                                                                                              >  1:4994000/1‑150 (MQ=255)

                                                           aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGTCTCGAGCTGCTGTCCGCTGGGAGCCGCGGGGGGCTGGCGGCCGTGCTCACTGCGcgcc                                                                                            >  2:889520/1‑150 (MQ=255)

                                                             caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGTAATCGGGCTGCTGAGCGCGGGTGTCCGCCCGCGTCGGGCGCCAGTACGCACTACGcgcccc                                                                                          >  1:2494548/1‑150 (MQ=255)

                                                             caaGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                          <  2:5159108/150‑1 (MQ=255)

                                                              aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCGCCCCCGGAGGGTGGGCGAGCTGCGGAGCGCGGGTAGCCGCCAGCGTGTGACGCCCGGACTCGCTACGcgccccg                                                                                         >  1:782686/1‑150 (MQ=255)

                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                                <  1:2029845/150‑1 (MQ=255)

                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                                <  1:1629742/150‑1 (MQ=255)

                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTGGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                                <  2:1424962/150‑1 (MQ=255)

                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTCCGCGAGCTGCTGGCCGCTGTTATCCGCCCGCAGCTGGCGCCCGGGCTCGCTCCGCGCCCCGCCCCCACTcca                                                                              >  1:3329577/1‑148 (MQ=255)

                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                             <  1:3319611/150‑1 (MQ=255)

                                                                          gATTTCTCGGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                             <  2:4515867/150‑1 (MQ=255)

                                                                              tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGTCAGAGAGCTGCTGACCGCTGAGATCCGCCCGCATCTGACGCCAGTACTCACTGCGCGCCCCGCCCCCACTCAACGTaa                                                                         >  2:4615952/1‑150 (MQ=255)

                                                                               ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGGGCGGGGGTCGGGCTGCGGACCGGGGTTGGCCGCGAGCGGCTGGCGCCGGTACGCACGCCGCGCCCCGCCCCCGGTCCACGGAac                                                                        >  2:1044270/1‑150 (MQ=255)

                                                                                tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCGCAATCAACGTAaca                                                                       <  2:2244297/150‑1 (MQ=255)

                                                                                   ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCGCCCCGGAGGGGAGTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGCCGCCGGTACTCGCTAGGCGGCCCGCCCCCGATCCACGGAacacgt                                                                    >  2:3541738/1‑148 (MQ=255)

                                                                                   ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGTAATCGAGCTGGTGACCGCTGATATCCGCCAGCAGCTGTCGCCAGTTCGCACTACGCGCCCCGCCCCCCCTCAACGTAACCCtt                                                                    >  2:2592294/1‑150 (MQ=255)

                                                                                    gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                                   <  1:1077699/150‑1 (MQ=255)

                                                                                     aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                                  <  1:1609589/150‑1 (MQ=255)

                                                                                     aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTCAGAGAGCTGCTGTCGGCTGTGATCCGCCCGCCGCTGACGCCAGTAGTCACTACGCGCCCCGCCCCCAAGCCACGTAACACTTTg                                                                  >  2:3076412/1‑150 (MQ=255)

                                                                                        cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                               <  2:4905543/150‑1 (MQ=255)

                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGACTCGGGCTGCTGACCGCGGCTGTCCGCCAGCCGCTGGCGCCAGTACTCGCGACGCGCCCCGCCCCCAATCCGCGTCACACGTTGCggg                                                              >  1:5133745/1‑149 (MQ=255)

                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt                                                              <  1:4968922/150‑1 (MQ=255)

                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                            <  2:3402544/150‑1 (MQ=255)

                                                                                             cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGAATCGCGCTGGTGTCCGCTGATATCCGCCAGCATCTGACGCCCGGACTCCCGACGCGCCCCGCCCCCAATCAACGGAGCACTTTGCGGGTTaa                                                          >  1:4268126/1‑150 (MQ=255)

                                                                                             cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGGCCCCCCCGGACGGTAATCGGGCTGGGGACCGCTGCTCTCCGCCCGCCTCTGACGCCGGGACTCCCTACGCGCCCCGCCCCCCATCCACGGTCCGCGTTGCGGttgac                                                          >  1:3716126/1‑147 (MQ=255)

                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:1873183/150‑1 (MQ=255)

                                                                                              gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:1876316/150‑1 (MQ=255)

                                                                                                  tGCGCCAGCCTTGCTGCGGCCAGAGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCAAGTACTCACTACGCGCCCCGCCCCCAATCAACGTATCACTTTGCGGTTTAATAcca                                                     <  2:2947113/150‑2 (MQ=255)

                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGTCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAAGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                                     <  2:1957887/150‑1 (MQ=255)

                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                                     <  1:181371/150‑1 (MQ=255)

                                                                                                     gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGTGGTCGAGCTGCGGGCCGCGGAGATCCGCGAGCAGCGGACGCCAGGACGCACGACGCGCCCCGCCCCCACTCACCGGAACCCGGTGGGGGTTAATACCGCAg                                                  >  2:416397/1‑150 (MQ=255)

                                                                                                       cAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGACGGTCATCGAGCTGCGGACCGCTGAGATCCGCCCGCGTCTGTCGCCCGGACTCACGACGCGCCCCGCCCCCCCTCGACGGACCGCGGTGCGGGGTAATGCCGCGGGc                                                >  2:2992675/1‑150 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGATCGGGCTGCTGGCCGCGGTTATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCAGCAACGTCACACGGTGCGGGTGAATACCGCAGGCATg                                             >  1:4302178/1‑150 (MQ=255)

                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGGTTAATACCGCAGGCATg                                             >  2:1694326/1‑150 (MQ=255)

                                                                                                            ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTGCTCACGACGCGCCCCGCCCCCGAGCAACGTCACACGTTGCGGGTTACTACCGCAggcaggca                                           >  2:167791/1‑150 (MQ=255)

                                                                                                            ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                           <  1:3902441/150‑1 (MQ=255)

                                                                                                             tgctgcGCCCAGTGCTGGCCCCACATCCTCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                          >  2:2804408/1‑150 (MQ=255)

                                                                                                                 gcgcCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGGACTCGAGCTGCGGACCGCTGATATCCGCCCGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAct                                      <  2:5110292/150‑2 (MQ=255)

                                                                                                                     ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                  <  1:2445099/150‑1 (MQ=255)

                                                                                                                     ccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                  >  2:2689696/1‑150 (MQ=255)

                                                                                                                      cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTTCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                 <  2:751237/150‑1 (MQ=255)

                                                                                                                      cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                 <  2:751274/150‑1 (MQ=255)

                                                                                                                       aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                                >  1:1473328/1‑150 (MQ=255)

                                                                                                                       aGTGCGGGCCCCACATCCCCCCCAGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCAGCCCCCAATCAACGTAACACTTTGAGGTTTAATACCGCAGGCATGCACGACATCCATg                                <  2:3642733/150‑1 (MQ=255)

                                                                                                                           cTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt                            <  2:3301038/150‑1 (MQ=255)

                                                                                                                            tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACGCACTACGCGCCCCGCCCCCAAACAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                           >  1:1100235/1‑150 (MQ=255)

                                                                                                                             ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                          <  1:617341/150‑1 (MQ=255)

                                                                                                                              gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTTCTCACTACGCGCCCCGCCCCCAATCAACCTAACACTTTGCGGTTTAATACCGCAGGCCTGCACGACATCCATGCCATCTg                         >  1:3696369/1‑150 (MQ=255)

                                                                                                                                  cacaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg                     >  2:3765488/1‑150 (MQ=255)

                                                                                                                                    caTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACCCTTTGCGGTTTAATACCGCAGGCATGCACGACCTCCCTGCCCTCTGCCAgcg                   >  2:3852541/1‑150 (MQ=255)

                                                                                                                                    caTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAACCATGCCATCTGCCAgcg                   >  1:3846695/1‑150 (MQ=255)

                                                                                                                                      tCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                 <  2:2017668/150‑1 (MQ=255)

                                                                                                                                         ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              <  1:2774361/150‑1 (MQ=255)

                                                                                                                                         ccccccGTACGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  2:9309/1‑150 (MQ=255)

                                                                                                                                         ccccccGTACGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  2:2627875/1‑150 (MQ=255)

                                                                                                                                           ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATCCCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc            <  1:139821/150‑1 (MQ=255)

                                                                                                                                             ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa          >  2:1406229/1‑150 (MQ=255)

                                                                                                                                             ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa          <  1:3006199/150‑1 (MQ=255)

                                                                                                                                             ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa          <  1:3006150/150‑1 (MQ=255)

                                                                                                                                                 aCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGcc      <  1:3493592/150‑1 (MQ=255)

                                                                                                                                                 aCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGcc      <  1:157212/150‑1 (MQ=255)

                                                                                                                                                  cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt     <  1:1127955/150‑1 (MQ=255)

                                                                                                                                                    gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc   >  2:4794790/1‑150 (MQ=255)

                                                                                                                                                     tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc  >  2:433930/1‑150 (MQ=255)

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721336‑3721634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`