Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,738,431 C→A 91.8% P90T (CCA→ACA)  yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,738,4310CA91.8% 853.3 / 56.1 268P90T (CCA→ACA) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base C (15/7);  new base A (133/113);  total (148/120)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 269 total aligned reads displayed.

CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  CP009273/3738284‑3738580
                                                                                                                                                   |                                                                                                                                                     
cAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACa                                                                                                                                                     >  2:2098661/1‑150 (MQ=255)
 aGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAg                                                                                                                                                    >  2:549264/1‑150 (MQ=255)
 aGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAg                                                                                                                                                    <  1:3233661/150‑1 (MQ=255)
  gATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGAGTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAgg                                                                                                                                                   <  1:3013476/150‑1 (MQ=255)
      aTGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCggg                                                                                                                                               <  1:3444906/150‑1 (MQ=255)
       tGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggc                                                                                                                                              >  1:1044555/1‑150 (MQ=255)
         gCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctg                                                                                                                                            <  2:3847069/150‑1 (MQ=255)
           acaAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCgggctggg                                                                                                                                          <  1:2814025/150‑1 (MQ=255)
               aCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTAc                                                                                                                                      >  2:3296138/1‑150 (MQ=255)
                   tGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCg                                                                                                                                  >  2:843062/1‑150 (MQ=255)
                     ntGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAATCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGc                                                                                                                                >  1:2456166/2‑150 (MQ=255)
                           ggAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTg                                                                                                                          >  1:4723812/1‑150 (MQ=255)
                             aGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGc                                                                                                                        >  1:4591093/1‑150 (MQ=255)
                               ccGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAg                                                                                                                      <  1:1692391/150‑1 (MQ=255)
                                 gATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                                                    >  1:3592746/1‑150 (MQ=255)
                                 gATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                                                    <  1:5092102/150‑1 (MQ=255)
                                   tAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAt                                                                                                                  >  2:4705397/1‑150 (MQ=255)
                                   tAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAt                                                                                                                  >  1:2722108/1‑150 (MQ=255)
                                    aGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATg                                                                                                                 >  2:1830092/1‑150 (MQ=255)
                                      cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                                               >  2:708052/1‑150 (MQ=255)
                                       ttCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAtt                                                                                                              >  2:1214815/1‑150 (MQ=255)
                                          tcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATg                                                                                                           >  1:4482176/1‑150 (MQ=255)
                                          tcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATg                                                                                                           <  1:4406252/150‑1 (MQ=255)
                                           cccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATgg                                                                                                          >  1:5130435/1‑150 (MQ=255)
                                            ccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGc                                                                                                         <  2:2948156/150‑1 (MQ=255)
                                              tgctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGa                                                                                                       <  1:4427722/150‑1 (MQ=255)
                                                ctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGc                                                                                                     >  2:4392539/1‑150 (MQ=255)
                                                 tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc                                                                                                    >  1:4781397/1‑150 (MQ=255)
                                                   gCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCtt                                                                                                  <  2:1839239/150‑1 (MQ=255)
                                                   gCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCtt                                                                                                  <  2:5169182/150‑1 (MQ=255)
                                                     gATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTc                                                                                                >  2:3341726/1‑150 (MQ=255)
                                                      aTTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCt                                                                                               <  1:4646355/150‑1 (MQ=255)
                                                        tCCGTTCTTTGTGCTGGCGGGTGAAATCATGAACGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTgg                                                                                             <  2:171602/150‑1 (MQ=255)
                                                           gTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTc                                                                                          <  1:3193780/150‑1 (MQ=255)
                                                               tttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGcc                                                                                      <  2:3986677/150‑1 (MQ=255)
                                                                 tgtgCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGt                                                                                    >  1:4052106/1‑150 (MQ=255)
                                                                   tgCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTg                                                                                  <  1:2177795/150‑1 (MQ=255)
                                                                    gCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGc                                                                                 <  2:3175365/150‑1 (MQ=255)
                                                                     cTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCg                                                                                >  1:3411040/1‑150 (MQ=255)
                                                                       ggCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGa                                                                              <  1:3670358/150‑1 (MQ=255)
                                                                       ggCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGa                                                                              <  2:453504/150‑1 (MQ=255)
                                                                           ggTGATATCATGACTGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGCTGAAACTGGTGGTTCATAAAACAGGCGGGCTGGGCTTCGTCGTCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAcc                                                                          <  1:2373398/150‑1 (MQ=255)
                                                                             tGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTGTCTGGTTCTGCCGTTGCGGATAccgc                                                                        >  2:1537344/1‑150 (MQ=255)
                                                                                aaTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgc                                                                     >  1:1103257/1‑150 (MQ=255)
                                                                                 aTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgcc                                                                    <  2:1027566/150‑1 (MQ=255)
                                                                                 aTCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgcc                                                                    <  2:1618887/150‑1 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                                 <  1:4861753/150‑1 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                                 >  1:847573/1‑150 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                                 >  2:251430/1‑150 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                                 >  2:2865435/1‑150 (MQ=255)
                                                                                    aTGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGtt                                                                 <  2:1388211/150‑1 (MQ=255)
                                                                                            gggcggCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGctct                                                         <  1:318277/150‑1 (MQ=255)
                                                                                               cggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                      >  2:3243992/1‑150 (MQ=255)
                                                                                               cggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                      <  1:1914845/150‑1 (MQ=255)
                                                                                               cggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                                                      >  2:4423207/1‑150 (MQ=255)
                                                                                                ggcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                                                     <  2:2387058/150‑1 (MQ=255)
                                                                                                 gcTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTgg                                                    >  1:3986616/1‑150 (MQ=255)
                                                                                                  cTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGt                                                   <  1:4801728/150‑1 (MQ=255)
                                                                                                   ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTg                                                  >  2:136240/1‑150 (MQ=255)
                                                                                                   ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTg                                                  >  2:136061/1‑150 (MQ=255)
                                                                                                   ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTg                                                  >  2:4109541/1‑150 (MQ=255)
                                                                                                   ttGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACGGGTg                                                  >  2:1513499/1‑150 (MQ=255)
                                                                                                       cAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCga                                              >  2:3420769/1‑150 (MQ=255)
                                                                                                        aaaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTCGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgat                                             >  1:4091539/1‑150 (MQ=255)
                                                                                                         aaaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg                                            >  2:858040/1‑150 (MQ=255)
                                                                                                          aaCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatga                                           <  1:846637/150‑1 (MQ=255)
                                                                                                           aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatgat                                          <  1:1282010/150‑1 (MQ=255)
                                                                                                           aCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatgat                                          <  2:1297499/150‑1 (MQ=255)
                                                                                                             gCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgc                                        >  1:2192767/1‑150 (MQ=255)
                                                                                                             gCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgc                                        >  1:2265690/1‑150 (MQ=255)
                                                                                                             gCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgc                                        >  2:4227081/1‑150 (MQ=255)
                                                                                                             gCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgc                                        >  1:4316241/1‑150 (MQ=255)
                                                                                                              cATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCCCAAACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcg                                       >  2:2579311/1‑150 (MQ=255)
                                                                                                               aTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc                                      <  2:2006049/150‑1 (MQ=255)
                                                                                                               aTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc                                      >  2:3617948/1‑150 (MQ=255)
                                                                                                                ttgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCa                                     <  2:3066498/150‑1 (MQ=255)
                                                                                                                 tgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAg                                    >  2:2581109/1‑150 (MQ=255)
                                                                                                                 tgttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAg                                    <  2:175260/150‑1 (MQ=255)
                                                                                                                  gttgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAgc                                   >  1:2759484/1‑150 (MQ=255)
                                                                                                                    tgACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAgcgc                                 >  1:17280/1‑150 (MQ=255)
                                                                                                                     gACCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGcc                                >  2:5105661/1‑150 (MQ=255)
                                                                                                                      aCCTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCa                               <  2:3874732/150‑1 (MQ=255)
                                                                                                                        cTGCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAAc                             >  1:143567/1‑150 (MQ=255)
                                                                                                                         tGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACt                            >  2:3501849/1‑150 (MQ=255)
                                                                                                                         tGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACt                            >  2:3502030/1‑150 (MQ=255)
                                                                                                                         tGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACt                            >  2:662592/1‑150 (MQ=255)
                                                                                                                          gCCGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTa                           >  1:5007152/1‑150 (MQ=255)
                                                                                                                            cGATGAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATc                         <  2:3574024/150‑1 (MQ=255)
                                                                                                                                gAAACTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGt                     <  1:36978/150‑1 (MQ=255)
                                                                                                                                   aCTGGTGGGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCaa                  <  2:119654/150‑1 (MQ=255)
                                                                                                                                     tggtggGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTCCTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAAcc                >  1:3331447/1‑150 (MQ=255)
                                                                                                                                        tggGGCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCggg             >  2:1895353/1‑150 (MQ=255)
                                                                                                                                           ggCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGggcgg          <  2:82821/150‑1 (MQ=255)
                                                                                                                                            gCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGggcggc         <  2:2192654/150‑1 (MQ=255)
                                                                                                                                            gCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGggcggc         <  1:4246231/150‑1 (MQ=255)
                                                                                                                                            gCATAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGggcggc         <  2:35049/150‑1 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGgcggcgc       >  2:4806365/1‑149 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg       >  1:4936869/1‑150 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg       >  1:52051/1‑150 (MQ=255)
                                                                                                                                              aTAAAACAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTAGCGGATACCGCCGCAGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGcggcgg       >  1:3935797/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTG  >  CP009273/3738284‑3738580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑