| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,816,601 | A→T | 100% | H281L (CAC→CTC) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,816,601 | 0 | A | T | 95.6% | 967.5 / 24.1 | 274 | H281L (CAC→CTC) | spoT | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (4/8); new base T (144/117); total (149/125) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 275 total aligned reads displayed.
GCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGA > CP009273/3816454‑3816747 | tCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGcc > 1:2873377/1‑150 (MQ=255) aGTGGTCGCGAGAAGCCTCTTTAGTCGATGTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCGACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTg < 2:5032023/150‑1 (MQ=255) tCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACaa > 1:1224232/1‑150 (MQ=255) cgcgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAg > 1:643828/1‑150 (MQ=255) cgcgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAg < 1:1981883/150‑1 (MQ=255) cgAGAAGCATCTTTATTCGATTTACTGCAAACTGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGcc < 2:4980684/150‑1 (MQ=255) gagaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCcg > 2:1340218/1‑150 (MQ=255) gagaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCcg > 1:1158998/1‑150 (MQ=255) gaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCcgcg > 1:3420143/1‑150 (MQ=255) aTCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCgg > 2:1834274/1‑150 (MQ=255) tCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCggg < 2:1900050/150‑1 (MQ=255) tCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCggg < 1:803039/150‑1 (MQ=255) tttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGcc < 1:5034227/150‑1 (MQ=255) gATTTACTGCAAAATGGTGCTCAAAGTGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaa > 2:870739/1‑150 (MQ=255) aTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa < 1:4165857/150‑1 (MQ=255) aTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa > 2:2853670/1‑150 (MQ=255) aTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa > 2:3181124/1‑150 (MQ=255) aTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa < 2:3290132/150‑1 (MQ=255) tttACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAg < 1:2599280/150‑1 (MQ=255) tACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGAc > 2:4616052/1‑150 (MQ=255) tACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGAc < 1:1512662/150‑1 (MQ=255) aCTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACt < 1:2931160/150‑1 (MQ=255) tGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACtat > 1:898132/1‑150 (MQ=255) cAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTatat < 1:3374073/150‑1 (MQ=255) tGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCa < 1:1801591/150‑1 (MQ=255) tGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCa < 1:4391772/150‑1 (MQ=255) gTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAtt < 1:4339452/150‑1 (MQ=255) aaaGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa > 1:1119492/1‑150 (MQ=255) aaaGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa > 1:1437293/1‑150 (MQ=255) aaGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAg > 2:42712/1‑150 (MQ=255) aaGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAg < 1:1710546/150‑1 (MQ=255) agagCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGc > 2:3881907/1‑150 (MQ=255) gagCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCg > 2:252861/1‑150 (MQ=255) gagCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCg < 1:428290/150‑1 (MQ=255) agcagcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGa < 1:1014128/150‑1 (MQ=255) cagcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGacc > 2:4439957/1‑148 (MQ=255) cagcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAAc < 2:2804398/150‑1 (MQ=255) agcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg > 2:2927105/1‑150 (MQ=255) agcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg > 2:2346079/1‑150 (MQ=255) agcGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg > 2:5047907/1‑150 (MQ=255) cGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGc > 1:3542127/1‑150 (MQ=255) cGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGc > 1:2415527/1‑150 (MQ=255) gTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCt > 2:3352276/1‑150 (MQ=255) ttttCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTa > 2:2562640/1‑150 (MQ=255) ttCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc < 2:3158461/150‑1 (MQ=255) cTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTc < 1:4440941/150‑1 (MQ=255) tCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt < 2:47444/150‑1 (MQ=255) tCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt > 2:2156265/1‑150 (MQ=255) tCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt < 2:1495042/150‑1 (MQ=255) tCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt < 1:269795/150‑1 (MQ=255) cGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCtt > 1:102225/1‑150 (MQ=255) gATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt > 1:2101475/1‑150 (MQ=255) aTCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTg < 2:4241653/150‑1 (MQ=255) cATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGca < 1:3882454/150‑1 (MQ=255) gACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACAcc > 2:2420184/1‑150 (MQ=255) aCATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCt > 2:4526866/1‑150 (MQ=255) cATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTc < 1:1056445/150‑1 (MQ=255) aTCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg > 2:541397/1‑150 (MQ=255) cTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgat > 2:1631753/1‑150 (MQ=255) cTAAGCTTTCCGCGTGATCGTAAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgat > 2:3707428/1‑150 (MQ=255) tCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGcc > 2:3465307/1‑150 (MQ=255) cgcgTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCg < 1:1151933/150‑1 (MQ=255) cgcgTGATCGACAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGACGATCGGCCCg < 1:685431/150‑1 (MQ=255) gTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCAc > 1:55770/1‑150 (MQ=255) gTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCAc > 1:3946869/1‑150 (MQ=255) gTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCAc > 1:252886/1‑150 (MQ=255) tCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGgtg < 1:2773304/150‑1 (MQ=255) cGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGgtgt < 1:369480/150‑1 (MQ=255) gTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGtgtg < 2:339293/150‑1 (MQ=255) cAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGcc < 1:5076084/150‑1 (MQ=255) aaTGATTCTGACACCTGTTCTCGCGTGCTGGGCCAGAGGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCg < 1:892482/150‑1 (MQ=255) aaTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCg < 1:1908004/150‑1 (MQ=255) aTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGAGCGGCCCGCACGGTGTGCCgg > 2:5167748/1‑150 (MQ=255) aTTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTg > 1:4583921/1‑150 (MQ=255) aTTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTg > 1:1846264/1‑150 (MQ=255) ttCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGa < 2:1623006/150‑1 (MQ=255) atCTGACACCAGTTATCTCGTGCTGGGCCAGATGCTCAGCCTGTACACGCCGCGTCCGGGCCGAGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCAATCAGTCTTAGCACACCTCGCTGATCGGCCCGCACGGTGTGCCGCTTGa > 2:3037884/2‑150 (MQ=255) cTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAgg < 2:1098660/150‑1 (MQ=255) tGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTAGATCGCCATTCCAAAAGCGAGCGGCTCTCTGGCTTTGCACACCGCGATGATCGGCCCGCACGGTGAGCCGGTGGAGGt > 2:1951253/1‑150 (MQ=255) gACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAATCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTc > 1:1754084/1‑150 (MQ=255) accccTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTcc < 1:4976004/147‑1 (MQ=255) cacCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCa > 2:4821238/1‑150 (MQ=255) acCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAg > 2:2918534/1‑150 (MQ=255) gTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc < 1:3443955/150‑1 (MQ=255) gTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATcc < 2:1135240/150‑1 (MQ=255) tATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAGTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGt < 1:2820917/150‑1 (MQ=255) cgcgTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTAcc > 1:206497/1‑150 (MQ=255) cgTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGa > 2:202366/1‑150 (MQ=255) gTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGaa < 2:3562621/150‑1 (MQ=255) gTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGaa > 2:3084367/1‑150 (MQ=255) gTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGaa < 2:2284939/150‑1 (MQ=255) tGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAg > 1:2226961/1‑150 (MQ=255) tGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAg < 2:105031/150‑1 (MQ=255) tGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCACGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAg > 1:4811651/1‑150 (MQ=255) gggCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGatat < 2:666033/150‑1 (MQ=255) cAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAc < 2:2774115/150‑1 (MQ=255) gATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCa > 2:639511/1‑150 (MQ=255) aTGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAg < 1:3181124/150‑1 (MQ=255) tGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGa < 2:1655283/150‑1 (MQ=255) tGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGa > 2:734171/1‑150 (MQ=255) | GCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGA > CP009273/3816454‑3816747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |