Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,340 A→C 13.2% Q12H (CAA→CAC)  xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3400AC13.2% 424.9 / 18.5 213Q12H (CAA→CAC) xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (109/70);  new base C (6/28);  total (115/98)
Fisher's exact test for biased strand distribution p-value = 3.30e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  CP009273/3822193‑3822489
                                                                                                                                                   |                                                                                                                                                     
 taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCg                                                                                                                                                    >  2:4636674/1‑150 (MQ=255)
 taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAccc                                                                                                                                                    >  1:1590846/1‑149 (MQ=255)
 taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAccc                                                                                                                                                    >  1:2749349/1‑149 (MQ=255)
 taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                    <  2:2555075/150‑1 (MQ=255)
  aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                   >  1:5099005/1‑150 (MQ=255)
   aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTCCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                  >  2:2720742/1‑150 (MQ=255)
   aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGt                                                                                                                                                  >  2:4210180/1‑150 (MQ=255)
   aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                  >  2:103937/1‑150 (MQ=255)
   aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                  >  1:3933043/1‑150 (MQ=255)
    acaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                 <  1:4232209/148‑1 (MQ=255)
    aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCCGtt                                                                                                                                                 >  1:2239589/1‑150 (MQ=255)
    aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                 >  2:1714545/1‑150 (MQ=255)
    aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                 >  1:3718246/1‑150 (MQ=255)
            aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                         >  1:2168130/1‑150 (MQ=255)
            aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGCTATCGCAGAc                                                                                                                                         >  2:1269853/1‑150 (MQ=255)
            aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGATTCGCAAAc                                                                                                                                         >  1:1009775/1‑150 (MQ=255)
            aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGGTGCGCCGAc                                                                                                                                         >  1:981911/1‑150 (MQ=255)
              ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTc                                                                                                                                       >  2:3974208/1‑150 (MQ=255)
              ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTc                                                                                                                                       >  2:3974270/1‑150 (MQ=255)
               tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACTTTTGCTTTTCTCGTTCGGGCCGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                      <  2:740409/150‑1 (MQ=255)
                aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaa                                                                                                                                     >  2:5148389/1‑150 (MQ=255)
                aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCaa                                                                                                                                     >  1:1227375/1‑150 (MQ=255)
                  ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCAGGTGCGCAGACACaaaa                                                                                                                                   >  1:2379828/1‑150 (MQ=255)
                   taaTTTAATTATTTAGTTGATGTTGTGGCAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACAACGGTATTGTTTTGGATGTCTTTTACCACCCTCGAGTAAGAAAATGCGCACCCGGTTGCGCAGACTCAAAAc                                                                                                                                  <  1:3423416/148‑1 (MQ=255)
                    cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                 <  2:5041133/150‑1 (MQ=255)
                      tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                               >  2:715026/1‑150 (MQ=255)
                            tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCACTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                         >  2:4870260/1‑150 (MQ=255)
                                 aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTa                                                                                                                    >  1:1164859/1‑150 (MQ=255)
                                       ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                              <  2:2747953/150‑1 (MQ=255)
                                           gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                          >  2:1592557/1‑150 (MQ=255)
                                               cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                      <  2:1864923/150‑1 (MQ=255)
                                                aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATCg                                                                                                     >  2:19492/1‑150 (MQ=255)
                                                 aaCGTTTGCGTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAACTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                    <  2:2791927/150‑1 (MQ=255)
                                                   cGTTTGCTTTTCTCTTTCGTTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                  <  2:545069/150‑1 (MQ=255)
                                                   cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                  <  2:3252170/150‑1 (MQ=255)
                                                    gTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                                 >  1:4604624/1‑150 (MQ=255)
                                                      ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcagcc                                                                                               >  2:4517099/1‑150 (MQ=255)
                                                      tggtttttCGCTGTCGGTCAGCTAAATGGCCTGTTTTGCTACGTGGGTTTTTTTTTGTTTGTGTGTTCCCACCCTCGAGTCATAAATGGCGCCCCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgcc                                                                                               <  1:1322375/146‑1 (MQ=255)
                                                               tctTGCGGTCAGCTAAACGGCCCGCTTTGCTACCACGGTTGTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAACTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                      <  1:821086/150‑1 (MQ=255)
                                                                ttttCGGTCATAAAAACTGCCCGGTTTGCCACCTCGGTTTTTTTTTCGTTTTTTGTTTCCACCCTCTATACAGAAAATGCCCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaa                                                                                     <  1:1975109/149‑1 (MQ=255)
                                                                 tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCGGTTGCGCACACGCAAAAACGCGCACAGATTTACCCTCTTGAAGATCGGCCGCCGCGTccgccaa                                                                                    >  1:1823230/1‑145 (MQ=255)
                                                                 tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                    <  2:4308493/150‑1 (MQ=255)
                                                                 tttCGGTCAGATAAAATGCCCGCTTTGCTACCAGGGGATTGTTTTCGTTGTGTGTTTCCACCCTCGAGTCATAAATTGCGCAACCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                    <  1:1479472/150‑1 (MQ=255)
                                                                   tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGCAGATCGTCCGCCGCTTCCTCAAAcc                                                                                  >  2:1531835/1‑150 (MQ=255)
                                                                    cGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCCGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATCGTACGCCGCGGCCGCAAAacc                                                                                 >  1:627378/1‑150 (MQ=255)
                                                                    cGGTCAGCTAAAAGGCCCGCTGTGCTACCTCGGTATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                 <  1:4763690/150‑1 (MQ=255)
                                                                      gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                               >  2:3324272/1‑150 (MQ=255)
                                                                      gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCCGACTAAAAACAGCGAACAGATTTAACGTCTTGAAGATAGTACCCCGCGTCCACAAAaccgg                                                                               >  1:924952/1‑148 (MQ=255)
                                                                         agtttttaTGCGCGCTTTGCTTCCACTGTTTTGTTTTCGTTGTGTGTTTCCACCCTCGTGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                            <  2:4061548/143‑1 (MQ=255)
                                                                         aGCTAAAGTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTGCCACCCTCGAGGCCGAGAATGCGCAACCGGTGGCGCAGACTCCAAACCGCGAGCTGATTGACCGTCTTGGAGATCGTCCGCCGCTGCCTCAGAcccgggtt                                                                            >  2:4541740/1‑145 (MQ=255)
                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATGGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCTTCCTCAAACCCGGtgt                                                                            >  2:3194784/1‑148 (MQ=255)
                                                                           cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                          <  2:2670085/150‑1 (MQ=255)
                                                                              aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTgccgc                                                                       >  2:1575675/1‑150 (MQ=255)
                                                                              aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                       >  2:3868775/1‑150 (MQ=255)
                                                                               aaggCCCGCTTTGCTACCACGGGATTGTTTTCGATTTCTGTTTCCACCCTCGAGTAATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                      <  2:889655/147‑1 (MQ=255)
                                                                               aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCGGTTTgccgcc                                                                      >  1:4703917/1‑150 (MQ=255)
                                                                                    ccGCTTTGCTACCACGGGATTGTTTTCGATGTCGGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                 <  2:3924818/150‑1 (MQ=255)
                                                                                      tcTTCGCTCCCCCGGGATTTTTTTCGTTGTCTGTTTCCACCCTCGCTTCAGAAAATGCGCAACGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                               <  1:4952850/149‑1 (MQ=255)
                                                                                      gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                               >  1:3376966/1‑150 (MQ=255)
                                                                                       cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATGTACCGTCTTGAAGATCGTCCGCCGCATCCTCAAACCCTGTTTGCCGACTGTCAGCa                                                              >  2:855585/1‑150 (MQ=255)
                                                                                         ttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                            <  2:5099005/150‑1 (MQ=255)
                                                                                         ttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTTAAGATCGTCCGCCGCGTCCGCAAACCCAGTTTGGCGCCTGTCAGCATc                                                            >  1:2648976/1‑150 (MQ=255)
                                                                                          tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                           >  2:3589693/1‑150 (MQ=255)
                                                                                          tGCAACCAGGGTTTTGTTTTCGTTGTCTGTTACCACCCTCGAGTCAGAAAATGCGCCCCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                           <  1:120868/150‑1 (MQ=255)
                                                                                             tACCCCGGGATTGTTTGCGAGGTCTGTTGCCACCCTCTAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgct                                                        <  1:4290450/150‑1 (MQ=255)
                                                                                              aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                       <  2:2176044/150‑1 (MQ=255)
                                                                                                cACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACGCAAAACAGCCAACAGATTTACCCTCTTGAAAATAGTACGCCGCGTCCACAAAACCAGTTTTACGCCAGTCACCCTCTGCTGGc                                                     >  1:572468/1‑150 (MQ=255)
                                                                                                    ggATTTTTTTCGTTGTGTGTTACCACCCTGGAGTCAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                 <  1:3181776/150‑1 (MQ=255)
                                                                                                    ggATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                 <  1:3771586/150‑1 (MQ=255)
                                                                                                    ggATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                 <  1:3472465/150‑1 (MQ=255)
                                                                                                     gATTGTGTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                                <  2:2828450/150‑1 (MQ=255)
                                                                                                       tttttttCGTTGTCTGTCTCCACCCTCGAGTCATAAAAGGCCCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                              <  1:528880/147‑1 (MQ=255)
                                                                                                        tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGCACAGATTTAACGGCTTGAAGATCGTCCCCCGCTTCCACAAACCCAGTTTGGCGCCGGTCAGCATCAGATGGCGATGTTCg                                             >  1:4919138/1‑150 (MQ=255)
                                                                                                        tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                             >  2:1533719/1‑150 (MQ=255)
                                                                                                        tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACGCAAAACAGCGAACTGATTTACCGTCATGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                             >  2:2312711/1‑150 (MQ=255)
                                                                                                         gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                            >  2:4670491/1‑150 (MQ=255)
                                                                                                         gTTGTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCACACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                            <  2:3054551/150‑1 (MQ=255)
                                                                                                              cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                       >  2:339844/1‑150 (MQ=255)
                                                                                                                aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                     <  2:3216539/150‑1 (MQ=255)
                                                                                                                  gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                   <  2:643933/150‑1 (MQ=255)
                                                                                                                  gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                   <  2:2181833/150‑1 (MQ=255)
                                                                                                                          ccACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCATCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                           >  2:3540457/1‑150 (MQ=255)
                                                                                                                          ccACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTGGCTGTGATCACGCCAg                           >  2:938444/1‑150 (MQ=255)
                                                                                                                            aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCAGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                         >  2:4428113/1‑150 (MQ=255)
                                                                                                                               cTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                      <  1:4287154/150‑1 (MQ=255)
                                                                                                                               cTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                      <  2:2379828/150‑1 (MQ=255)
                                                                                                                                  gAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCAAATGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTa                   >  1:4328061/1‑150 (MQ=255)
                                                                                                                                  gAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACTGATTTACCCTCTTGAAGATAGTCCGCCGCTTCCACAAAACCAGTTTGCCGCCGGTCAGCAACTGCTGGCGAAGTTAGGTGCGGTGATAACGCCAGCGCGATTa                   >  1:3988386/1‑150 (MQ=255)
                                                                                                                                   agtaataaaaTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa                  <  1:4636695/144‑1 (MQ=255)
                                                                                                                                      cAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt               <  2:3632202/150‑1 (MQ=255)
                                                                                                                                       aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg              >  2:1566863/1‑150 (MQ=255)
                                                                                                                                        gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc             >  2:1433183/1‑150 (MQ=255)
                                                                                                                                         aaacTGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc            <  1:4746048/150‑1 (MQ=255)
                                                                                                                                         aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGGTGCGGTGATCACGCCAGCGCTATTAATCTGcc            >  1:1789984/1‑150 (MQ=255)
                                                                                                                                         aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTGACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc            <  2:1150428/150‑1 (MQ=255)
                                                                                                                                          aaaTGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATGTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           <  2:571923/150‑1 (MQ=255)
                                                                                                                                          aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           >  1:438113/1‑150 (MQ=255)
                                                                                                                                           aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTGGCGGTGATCACGCCAGCGCTATTAATCTGCCAg          >  2:5043861/1‑150 (MQ=255)
                                                                                                                                             tGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc        >  1:2894869/1‑150 (MQ=255)
                                                                                                                                               cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc      >  2:2369050/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  CP009273/3822193‑3822489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑