Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,844,801 C→A 5.2% M448I (ATG→ATT)  ilvB ← acetolactate synthase 2 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,844,8010CA 5.2% 813.9 / 19.8 231M448I (ATG→ATT) ilvBacetolactate synthase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base C (115/103);  new base A (7/5);  total (122/109)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

ATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCG  >  CP009273/3844652‑3844949
                                                                                                                                                     |                                                                                                                                                    
 ttttGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATtcatca                                                                                                                                                     <  1:4039171/150‑1 (MQ=255)
         ggATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTg                                                                                                                                             >  1:3813587/1‑150 (MQ=255)
           aTAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGcc                                                                                                                                           <  1:4077704/150‑1 (MQ=255)
           aTAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGcc                                                                                                                                           <  1:539057/150‑1 (MQ=255)
            tAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCg                                                                                                                                          <  2:1786691/150‑1 (MQ=255)
             aGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGt                                                                                                                                         >  2:2819596/1‑150 (MQ=255)
                tGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGc                                                                                                                                      >  1:4297059/1‑150 (MQ=255)
                  gcggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCg                                                                                                                                    <  2:4537966/150‑1 (MQ=255)
                    ggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGga                                                                                                                                  <  2:2243029/150‑1 (MQ=255)
                    ggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGga                                                                                                                                  >  2:2288285/1‑150 (MQ=255)
                     gcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGgag                                                                                                                                 >  1:4290970/1‑150 (MQ=255)
                       aaaaaCGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGaa                                                                                                                               >  2:4590659/1‑150 (MQ=255)
                          aaCGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAcaca                                                                                                                            >  1:1541506/1‑150 (MQ=255)
                            cGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAaacaca                                                                                                                          >  1:4121606/1‑150 (MQ=255)
                             gCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGACTATTCATCATCAGGCTGCCGTCGCCGGAGAaacacaa                                                                                                                         >  2:582321/1‑150 (MQ=255)
                             gCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAaacacaa                                                                                                                         <  2:342359/150‑1 (MQ=255)
                              ccTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAaacacaac                                                                                                                        <  1:490854/150‑1 (MQ=255)
                               cTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATTATCAGGCTGCCGTCGCCGGAGAaacacaaca                                                                                                                       <  1:3845125/150‑1 (MQ=255)
                                ttGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAaacacaacac                                                                                                                      >  1:5126407/1‑150 (MQ=255)
                                   cTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACttt                                                                                                                   <  1:4590659/150‑1 (MQ=255)
                                   cTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACttt                                                                                                                   <  2:446011/150‑1 (MQ=255)
                                     cGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGc                                                                                                                 <  2:1984591/150‑1 (MQ=255)
                                      gTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCg                                                                                                                >  2:355283/1‑150 (MQ=255)
                                       tAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGa                                                                                                               >  2:1443811/1‑150 (MQ=255)
                                       tAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGa                                                                                                               >  1:1928058/1‑150 (MQ=255)
                                          aaCAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATcc                                                                                                            >  1:4843213/1‑150 (MQ=255)
                                             aGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCggg                                                                                                         >  1:1345491/1‑150 (MQ=255)
                                              gACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATAATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGt                                                                                                        <  1:4560824/150‑1 (MQ=255)
                                                ctctGTTGCTGATGCACCAGCCCCCGCGCCTCGTTGTTCATCAGAATGATTTTGACCTCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGGTc                                                                                                      <  1:3180083/150‑1 (MQ=255)
                                                ctctGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTc                                                                                                      <  2:3749699/150‑1 (MQ=255)
                                                 tctGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCg                                                                                                     >  1:2367903/1‑150 (MQ=255)
                                                 tctGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCg                                                                                                     >  1:2367740/1‑150 (MQ=255)
                                                  ctGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGc                                                                                                    <  1:3414288/150‑1 (MQ=255)
                                                  ctGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGc                                                                                                    >  1:386250/1‑150 (MQ=255)
                                                   tGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGATTGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGcc                                                                                                   <  2:3823003/150‑1 (MQ=255)
                                                   tGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGcc                                                                                                   <  2:3061586/150‑1 (MQ=255)
                                                   tGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGcc                                                                                                   <  1:676533/150‑1 (MQ=255)
                                                     ttGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATAATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCcag                                                                                                 >  2:83115/1‑150 (MQ=255)
                                                         tGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCcagcgca                                                                                             <  1:4384344/150‑1 (MQ=255)
                                                           aTGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCcagcgcagc                                                                                           >  2:4283746/1‑150 (MQ=255)
                                                               aCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCa                                                                                       >  1:2922311/1‑150 (MQ=255)
                                                               aCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCa                                                                                       >  2:2092029/1‑150 (MQ=255)
                                                               aCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCa                                                                                       >  1:2922550/1‑150 (MQ=255)
                                                                ccAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCaa                                                                                      >  1:5146134/1‑150 (MQ=255)
                                                                ccAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATAATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCaa                                                                                      <  2:2841888/150‑1 (MQ=255)
                                                                   gCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATcg                                                                                   >  1:2169187/1‑150 (MQ=255)
                                                                       cAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATcgccgc                                                                               <  2:309724/150‑1 (MQ=255)
                                                                         gcgcTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCgcag                                                                             <  2:510803/150‑1 (MQ=255)
                                                                         gcgcTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCgcag                                                                             <  2:510672/150‑1 (MQ=255)
                                                                          cgcTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCgcagg                                                                            >  2:3512185/1‑150 (MQ=255)
                                                                          cgcTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCgcagg                                                                            <  1:4050314/150‑1 (MQ=255)
                                                                              tCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGcaggcagg                                                                        <  1:2895290/150‑1 (MQ=255)
                                                                               cGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATAATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCCGCGCAGCGCCAATCGCCGCaggcaggc                                                                       >  2:1861675/1‑150 (MQ=255)
                                                                                gttgttCATCAGTATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGcc                                                                      <  1:1130776/150‑1 (MQ=255)
                                                                                gttgttCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGcc                                                                      >  2:4995597/1‑150 (MQ=255)
                                                                                    ttCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCaaaa                                                                  <  1:1677732/150‑1 (MQ=255)
                                                                                    ttCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCaaaa                                                                  >  2:1995323/1‑150 (MQ=255)
                                                                                    ttCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCAACATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCaaaa                                                                  <  1:1677564/150‑1 (MQ=255)
                                                                                           gAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCg                                                           >  2:44869/1‑150 (MQ=255)
                                                                                            aaTGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGt                                                          <  2:1868580/150‑1 (MQ=255)
                                                                                              tGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgc                                                        >  1:3290773/1‑150 (MQ=255)
                                                                                               gATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgcc                                                       <  1:3388809/150‑1 (MQ=255)
                                                                                               gATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgcc                                                       >  1:1429610/1‑150 (MQ=255)
                                                                                                aTTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgccc                                                      >  1:96637/1‑150 (MQ=255)
                                                                                                aTTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgccc                                                      >  2:4637366/1‑150 (MQ=255)
                                                                                                 ttttGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgccca                                                     <  1:758140/150‑1 (MQ=255)
                                                                                                 ttttGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATAATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTgccca                                                     <  1:43193/150‑1 (MQ=255)
                                                                                                        aTCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCAcc                                              <  2:4860679/150‑1 (MQ=255)
                                                                                                        aTCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCAcc                                              >  1:2024154/1‑150 (MQ=255)
                                                                                                          ccAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCgg                                            <  2:4795176/150‑1 (MQ=255)
                                                                                                            aGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAg                                          <  1:4239040/150‑1 (MQ=255)
                                                                                                               tGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCCTCAGGCTGCCGTCGCCGGAGAAACACAACACTGTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTc                                       <  2:2644928/150‑1 (MQ=255)
                                                                                                                 aTTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAg                                     <  2:1408422/150‑1 (MQ=255)
                                                                                                                   tttCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGcc                                   <  2:946107/150‑1 (MQ=255)
                                                                                                                   tttCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGcc                                   <  1:4112609/150‑1 (MQ=255)
                                                                                                                     tCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCAc                                 <  1:5020461/150‑1 (MQ=255)
                                                                                                                      cACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACt                                >  1:3703642/1‑150 (MQ=255)
                                                                                                                       aCTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTg                               >  2:1257289/1‑150 (MQ=255)
                                                                                                                        cTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTgg                              >  2:715631/1‑150 (MQ=255)
                                                                                                                         tGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGc                             >  2:3168264/1‑150 (MQ=255)
                                                                                                                         tGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGc                             >  2:5166176/1‑150 (MQ=255)
                                                                                                                         tGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGc                             <  2:3176493/150‑1 (MQ=255)
                                                                                                                          ggCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCg                            >  1:3485083/1‑150 (MQ=255)
                                                                                                                           gCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGACACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGt                           <  2:4169818/150‑1 (MQ=255)
                                                                                                                             ggTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTgg                         >  1:1639431/1‑150 (MQ=255)
                                                                                                                             ggTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTgg                         >  2:3951005/1‑150 (MQ=255)
                                                                                                                              gTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTggg                        <  1:2092029/150‑1 (MQ=255)
                                                                                                                                   cATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGAtt                   >  2:2852247/1‑150 (MQ=255)
                                                                                                                                   cATCTCCTGAATATCCAGCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGAtt                   <  2:5132128/150‑1 (MQ=255)
                                                                                                                                      ctcCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAg                >  2:2551399/1‑150 (MQ=255)
                                                                                                                                      ctcCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAg                >  2:3351916/1‑150 (MQ=255)
                                                                                                                                             atatTCATAATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATaa         <  1:2480723/150‑1 (MQ=255)
                                                                                                                                               atTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGc       <  2:2418364/150‑1 (MQ=255)
                                                                                                                                               atTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACGTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGc       <  2:4113733/150‑1 (MQ=255)
                                                                                                                                                ttCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCt      <  2:2443794/150‑1 (MQ=255)
                                                                                                                                                 tcatcatcaGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCtt     <  1:2991141/150‑1 (MQ=255)
                                                                                                                                                  cataatcaGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTg    <  2:4275059/150‑1 (MQ=255)
                                                                                                                                                   atcatcaGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTgc   <  1:2651558/150‑1 (MQ=255)
                                                                                                                                                   atcatcaGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTgc   >  1:588853/1‑150 (MQ=255)
                                                                                                                                                   atcatcaGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTgc   <  1:187928/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGCCAGCGCAGCGCCAATCGCCGCAGGCAGGCCAAAACCCATCGTGCCCAGCCCACCGGAGGTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCG  >  CP009273/3844652‑3844949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑