Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,849,854 A→T 25.3% H27L (CAC→CTC)  yidI → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,849,8540AT25.3% 470.5 / 186.5 229H27L (CAC→CTC) yidIinner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (93/78);  new base T (34/24);  total (127/102)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

CGGTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATA  >  CP009273/3849707‑3850003
                                                                                                                                                   |                                                                                                                                                     
cGGTTTTTTTATGTATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCTCTACAAGGAGGTCAGTTGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCctct                                                                                                                                                     <  2:590528/150‑1 (MQ=255)
 ggTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACtt                                                                                                                                                    >  1:496347/1‑150 (MQ=255)
    ttttttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTc                                                                                                                                                 <  1:4315177/150‑1 (MQ=255)
      ttttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCAt                                                                                                                                               <  2:4432428/150‑1 (MQ=255)
       tttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCAtt                                                                                                                                              <  1:15030/150‑1 (MQ=255)
        ttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTc                                                                                                                                             >  1:2570348/1‑150 (MQ=255)
         tAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTca                                                                                                                                            <  2:5122617/150‑2 (MQ=255)
         tAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCg                                                                                                                                            >  1:1531411/1‑150 (MQ=255)
          aaGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCgg                                                                                                                                           <  2:4513199/150‑1 (MQ=255)
          aaGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCgg                                                                                                                                           >  1:2909379/1‑150 (MQ=255)
            gAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGCTGGGGATTATCCACTTTTCATTCGGcc                                                                                                                                         >  2:3092850/1‑150 (MQ=255)
            gAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGcc                                                                                                                                         >  2:4327007/1‑150 (MQ=255)
             aaTTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGccc                                                                                                                                        >  2:944000/1‑150 (MQ=255)
               tttCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCtt                                                                                                                                      >  1:4057198/1‑150 (MQ=255)
                 tCGTGCGCCCGTTTGCTGATATGCTTTTGCCATCCGCTACTAGGTGGTCAGATGGGTATCATATCTCAAAATAAAATCAGTTCTTTGGGCATGGTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTtc                                                                                                                                    <  1:1978023/150‑1 (MQ=255)
                   gTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTtctc                                                                                                                                  >  1:2397265/1‑150 (MQ=255)
                    tCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTcc                                                                                                                                 <  2:4775737/150‑1 (MQ=255)
                    tCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTcc                                                                                                                                 >  2:4189346/1‑150 (MQ=255)
                    tCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTcc                                                                                                                                 <  1:489638/150‑1 (MQ=255)
                     ccGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCg                                                                                                                                >  2:3318839/1‑150 (MQ=255)
                     ccGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCg                                                                                                                                >  1:2092577/1‑150 (MQ=255)
                       gCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGca                                                                                                                              >  1:2680983/1‑150 (MQ=255)
                       gCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGca                                                                                                                              <  1:4131483/150‑1 (MQ=255)
                        cccGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGcac                                                                                                                             >  2:4037960/1‑150 (MQ=255)
                         ccGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGcacc                                                                                                                            <  1:2233993/150‑1 (MQ=255)
                          cGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGcacca                                                                                                                           >  1:104255/1‑150 (MQ=255)
                           gTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGcaccac                                                                                                                          >  1:726119/1‑150 (MQ=255)
                           gTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGcaccac                                                                                                                          >  1:4172923/1‑150 (MQ=255)
                               ccTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGcc                                                                                                                      <  1:343634/150‑1 (MQ=255)
                                  gATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAAc                                                                                                                   <  2:2265097/150‑1 (MQ=255)
                                         ttttGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGaa                                                                                                            <  2:2383658/150‑1 (MQ=255)
                                         ttttGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGaa                                                                                                            <  2:1329629/150‑1 (MQ=255)
                                              ccATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCAt                                                                                                       >  1:1855378/1‑150 (MQ=255)
                                               cATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATc                                                                                                      <  2:153381/150‑1 (MQ=255)
                                                aTCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCg                                                                                                     >  1:1321683/1‑150 (MQ=255)
                                                aTCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCg                                                                                                     >  1:4408007/1‑150 (MQ=255)
                                                aTCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCg                                                                                                     <  1:658321/150‑1 (MQ=255)
                                                aTCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCg                                                                                                     >  1:1819184/1‑150 (MQ=255)
                                                 tCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGt                                                                                                    >  2:1145593/1‑150 (MQ=255)
                                                  ccGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTg                                                                                                   <  2:4893322/150‑1 (MQ=255)
                                                  ccGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTg                                                                                                   >  1:5121852/1‑150 (MQ=255)
                                                   cGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTgg                                                                                                  >  2:339494/1‑150 (MQ=255)
                                                     cTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCg                                                                                                >  2:1969832/1‑150 (MQ=255)
                                                      tACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCgg                                                                                               <  1:2498830/150‑1 (MQ=255)
                                                             aggTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAAc                                                                                        <  1:1584222/150‑1 (MQ=255)
                                                              ggTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACg                                                                                       <  2:19973/150‑1 (MQ=255)
                                                                 cAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCt                                                                                    >  2:3698375/1‑150 (MQ=255)
                                                                  aGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTg                                                                                   <  2:1799799/150‑1 (MQ=255)
                                                                   gATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGa                                                                                  >  1:13327/1‑150 (MQ=255)
                                                                     tGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGaaa                                                                                >  1:4741884/1‑150 (MQ=255)
                                                                     tGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGaaa                                                                                <  1:4603690/150‑1 (MQ=255)
                                                                     tGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGaaa                                                                                >  1:3822269/1‑150 (MQ=255)
                                                                      gggTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAAt                                                                               <  1:4814222/150‑1 (MQ=255)
                                                                      gggTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAAt                                                                               >  1:4998252/1‑150 (MQ=255)
                                                                      gggTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAAt                                                                               >  2:709638/1‑150 (MQ=255)
                                                                         tATCATAGATCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTaa                                                                            <  1:4109086/150‑1 (MQ=255)
                                                                            cATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAag                                                                         >  1:964536/1‑150 (MQ=255)
                                                                                  tCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtct                                                                   <  2:762431/150‑1 (MQ=255)
                                                                                   cAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtctt                                                                  <  1:1866110/150‑1 (MQ=255)
                                                                                    aaaataaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtcttc                                                                 <  2:3230723/150‑1 (MQ=255)
                                                                                     aaataaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtcttct                                                                <  1:139685/150‑1 (MQ=255)
                                                                                      aataaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTtctc                                                               >  2:278974/1‑150 (MQ=255)
                                                                                         aaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGcc                                                            <  1:881495/150‑1 (MQ=255)
                                                                                         aaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGcc                                                            >  2:847575/1‑150 (MQ=255)
                                                                                          aaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCg                                                           >  2:12662/1‑150 (MQ=255)
                                                                                          aaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCg                                                           <  2:2097964/150‑1 (MQ=255)
                                                                                          aaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTAGAAAGCATCGTGGCGGACAAAACGGCTGAAAATAAAAGACGTCTTCTCGCCg                                                           >  2:914967/1‑150 (MQ=255)
                                                                                             tCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGaa                                                        <  1:692040/150‑1 (MQ=255)
                                                                                              cAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAAt                                                       >  2:2896405/1‑150 (MQ=255)
                                                                                               aGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATa                                                      >  2:2373404/1‑150 (MQ=255)
                                                                                                gTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATaa                                                     >  1:3371865/1‑150 (MQ=255)
                                                                                                gTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATaa                                                     <  2:1515688/150‑1 (MQ=255)
                                                                                                   cATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAACACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAg                                                  <  2:4886044/150‑1 (MQ=255)
                                                                                                    aTTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAgg                                                 >  2:1437693/1‑150 (MQ=255)
                                                                                                      tGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCg                                               >  2:681135/1‑150 (MQ=255)
                                                                                                      tGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCg                                               >  2:680928/1‑150 (MQ=255)
                                                                                                          cATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGaaaa                                           <  1:1068002/150‑1 (MQ=255)
                                                                                                            tGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGaaaaaa                                         >  2:3714738/1‑150 (MQ=255)
                                                                                                              cTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAAtt                                       >  2:2060407/1‑150 (MQ=255)
                                                                                                              cTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAAtt                                       >  1:357165/1‑150 (MQ=255)
                                                                                                                    ggTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccacc                                 <  2:384226/150‑1 (MQ=255)
                                                                                                                    ggTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccacc                                 <  2:3586697/150‑1 (MQ=255)
                                                                                                                         cATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGga                            >  1:4244868/1‑150 (MQ=255)
                                                                                                                         cATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGga                            <  2:4330829/150‑1 (MQ=255)
                                                                                                                         cATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCGTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGga                            <  2:5092172/150‑1 (MQ=255)
                                                                                                                         cATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTGTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGga                            <  2:5092209/150‑1 (MQ=255)
                                                                                                                           tCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACTGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGgaga                          >  2:2647411/1‑150 (MQ=255)
                                                                                                                           tCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGgaga                          >  1:2578883/1‑150 (MQ=255)
                                                                                                                               tttAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCG‑AAAAATTACCACCGTGGAGaaaaaa                     >  1:2755211/1‑150 (MQ=255)
                                                                                                                                 tAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAag                    <  1:4515039/150‑1 (MQ=255)
                                                                                                                                    tgatgGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAgg                 >  2:646448/1‑150 (MQ=255)
                                                                                                                                     gatgGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGa                <  1:1094215/150‑1 (MQ=255)
                                                                                                                                        ggggATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGt             >  1:1836070/1‑150 (MQ=255)
                                                                                                                                          ggATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAg           <  2:3776306/150‑1 (MQ=255)
                                                                                                                                            aTTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGAt         >  1:2369019/1‑150 (MQ=255)
                                                                                                                                              tATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGt       >  2:3828203/1‑150 (MQ=255)
                                                                                                                                              tATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGt       <  1:1426963/150‑1 (MQ=255)
                                                                                                                                               aTCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTc      >  2:1745724/1‑150 (MQ=255)
                                                                                                                                                tCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCg     <  1:3941943/150‑1 (MQ=255)
                                                                                                                                                   aCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATa  <  1:1351231/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATA  >  CP009273/3849707‑3850003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑