BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,159	C→A	93.7%	P33P (CCG→CCT)	ibpB ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,159	0	C	A	93.7%	604.5 / 28.7	230	P33P (CCG→CCT)	ibpB	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (5/10); new base A (94/115); total (105/125)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

Only 100 of 233 total aligned reads displayed.

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGA  >  CP009273/3860013‑3860305

     ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGct                                                                                                                                            >  2:2377954/1‑150 (MQ=255)

     ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGct                                                                                                                                            <  1:1877819/150‑1 (MQ=255)

        ttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTg                                                                                                                                         <  2:1758962/150‑1 (MQ=255)

              tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTc                                                                                                                                   >  2:4270038/1‑150 (MQ=255)

               ggCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCa                                                                                                                                  <  2:2181156/150‑1 (MQ=255)

               ggCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCa                                                                                                                                  <  2:4860762/150‑1 (MQ=255)

                  tgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTTTACGGAGGGAATCTCTGGGTGTGACCg                                                                                                                               >  1:687555/1‑150 (MQ=255)

                    ctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCAGGCTTTCACCGGc                                                                                                                             >  1:2856813/1‑150 (MQ=255)

                     tCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCAAGTTGTACGGCGGGAACCTCTGGCGTTAACCGGCg                                                                                                                            >  1:4893350/1‑150 (MQ=255)

                     tCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAAGGAGGGAAGCTCAGGCGTTCAcacgcg                                                                                                                            >  1:4368630/1‑145 (MQ=255)

                      ccGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGCTCTGGCTTTAACCGGCGt                                                                                                                           >  2:894672/1‑150 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          >  2:4767666/1‑150 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          <  2:2832955/150‑1 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          <  2:848051/150‑1 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTACGGAGGGAAGCTCTGGGTTTCACCGGCggt                                                                                                                          >  2:1010363/1‑148 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                          >  1:3894650/1‑150 (MQ=255)

                        ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTTCGGAGGGAAGCTCTGGCTTTCACCGGCGttt                                                                                                                         >  1:5054660/1‑150 (MQ=255)

                        ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGgtt                                                                                                                         >  1:2519807/1‑150 (MQ=255)

                         gCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtttt                                                                                                                        <  1:4825759/150‑1 (MQ=255)

                         gCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtttt                                                                                                                        <  1:882367/150‑1 (MQ=255)

                          cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGGTTTg                                                                                                                       >  2:1375502/1‑150 (MQ=255)

                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGc                                                                                                                      <  1:142779/150‑1 (MQ=255)

                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTAAGGAGGGAAGCTCTGGCGTTCACCGGCGtttttc                                                                                                                      >  1:3212706/1‑148 (MQ=255)

                             gCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAg                                                                                                                    <  1:2501601/150‑1 (MQ=255)

                             gCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAg                                                                                                                    <  2:3474404/150‑1 (MQ=255)

                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGc                                                                                                                 <  1:967200/150‑1 (MQ=255)

                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGc                                                                                                                 >  2:2168764/1‑150 (MQ=255)

                                  ttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTTATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                               <  2:3103348/150‑1 (MQ=255)

                                  ttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                               <  1:1022466/150‑1 (MQ=255)

                                   tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGtt                                                                                                              <  2:4210809/150‑1 (MQ=255)

                                    aCGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTg                                                                                                             <  2:5170703/150‑1 (MQ=255)

                                     cGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTgg                                                                                                            <  2:5044641/150‑1 (MQ=255)

                                       cTCAGGCGCGAACCAGCCAGATGAATCTCGAAATCTTCCCGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCGGGCTTTCACCGGCGTTTTGCAGTGCGTTGGcc                                                                                                          <  2:3485868/150‑1 (MQ=255)

                                         cAGTCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCAGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGTTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTTGGCCAg                                                                                                        <  2:3170973/150‑1 (MQ=255)

                                         cAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAg                                                                                                        <  2:4561742/150‑1 (MQ=255)

                                         cAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTATATAAAAGGTGTAAGGAAGGAAGCTCTGGCATTAAACCGCGTTTTGCAGTGCGGAGGCCAg                                                                                                        >  1:849981/1‑150 (MQ=255)

                                           ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGtt                                                                                                      <  2:1194186/150‑1 (MQ=255)

                                             cgcgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGGTTg                                                                                                    >  2:4101862/1‑150 (MQ=255)

                                               cgTACCTTCCAGTTGAATCTCTACATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                  <  2:476359/150‑1 (MQ=255)

                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCaa                                                                                                <  1:3227243/150‑1 (MQ=255)

                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTGTTGCAGTGCGGTGGCCCGGTTGTcca                                                                                                >  2:2073936/1‑148 (MQ=255)

                                                 tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCCGTGCGTTGGCCAGTTTGTCaa                                                                                                >  2:2073414/1‑150 (MQ=255)

                                                  aCCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCaaa                                                                                               <  1:5172491/150‑1 (MQ=255)

                                                         aGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCGTTTGTCAAAACCGATc                                                                                        >  1:4211863/1‑150 (MQ=255)

                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTTTAAGGGGGGAAGCTCTGGCTTTACACGGCGTTTTTCAGTTCGTTTGGCAGGTTGTGAAAACCCAtac                                                                                       >  2:1570944/1‑148 (MQ=255)

                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCCGTGCGTTGGCCCGGTTGTCAAAACCGAtac                                                                                       >  2:277339/1‑148 (MQ=255)

                                                           ttGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTTTCAAAACCGATCCa                                                                                      >  2:723391/1‑150 (MQ=255)

                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGCGGGGAGCTCAGGCTTTAAACGGCGTTTTTCAGTGCGTTGGCCAGGTTGTCAAAACCGATCCaat                                                                                    >  2:601884/1‑148 (MQ=255)

                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAGTTTGTACGGCGGGAAGCTCAGGCATTCACCCGCGTTTTGCAGTGCGTTGGGCCGGTTTTCAAAACCGATACaat                                                                                    >  1:1085412/1‑148 (MQ=255)

                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGCAGCACTGGCTTTCAACGGCGTTTTGCAGTTCGTTGGCCCGTTTGTCAAAAACGCTCCaat                                                                                    >  1:1641706/1‑148 (MQ=255)

                                                              aaTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTg                                                                                   <  1:2219085/150‑1 (MQ=255)

                                                               aTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGa                                                                                  <  1:2632367/150‑1 (MQ=255)

                                                                    tAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGTGCAGTTTGTCAAAAACGATACAATGACGCAt                                                                             >  1:3759991/1‑150 (MQ=255)

                                                                    tAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGGGGGAAGCTCTGGCTTTCACCGGCGGGTTTGAGTGCGTTGGCCCGTTTTTAAAAAACCATACCATGACGCaa                                                                             >  1:659957/1‑149 (MQ=255)

                                                                        tCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGt                                                                         <  1:4962220/150‑1 (MQ=255)

                                                                        tCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCCTTTGTCAAAACCGATCCATTTACGCATCCGt                                                                         >  1:2988858/1‑150 (MQ=255)

                                                                             cTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGa                                                                    <  2:1573437/150‑1 (MQ=255)

                                                                             cTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGTTTGTCAAAACCGATACATTGACGCATCAGTGGGGa                                                                    >  2:428862/1‑150 (MQ=255)

                                                                              tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAt                                                                   <  1:1163763/150‑1 (MQ=255)

                                                                              tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAt                                                                   <  2:3099082/150‑1 (MQ=255)

                                                                              tGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGGTTTGCAGTGCGTTGGCCAGGTTGTCAAAACCGATCCATTGACGCATCAGGGGGGAt                                                                   >  2:2150020/1‑150 (MQ=255)

                                                                                  ggAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAAt                                                               <  2:1521814/150‑1 (MQ=255)

                                                                                   gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                              <  2:1790908/150‑1 (MQ=255)

                                                                                     aaCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTTCGGAGGGAAGCACAGGCGTTCACCGGCGGTTTGCACGGCGGTTGCCCGGTTGTCAAAACCCATACCATGACGCAGCAGAGGGGGTAAATCGa                                                            >  2:4948457/1‑150 (MQ=255)

                                                                                         tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGtt                                                        <  1:1731601/150‑1 (MQ=255)

                                                                                            cAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACg                                                     <  1:3642431/150‑1 (MQ=255)

                                                                                                gcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATa                                                 <  1:2896742/150‑1 (MQ=255)

                                                                                                 cAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAg                                                <  2:941279/150‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:4408842/150‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:4467045/150‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:2099206/150‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:475424/150‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                             <  1:4562552/150‑1 (MQ=255)

                                                                                                     ggTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTACGGTGGGCAGCTATGGCGTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTTTCAAAACCCATCCATTTTAGCATCAGTGGGGATAAAAAGAAGTTACGCAAAGTcct                                            >  1:5115577/1‑148 (MQ=255)

                                                                                                     ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAACGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATATTCAt                                            >  2:3682894/1‑150 (MQ=255)

                                                                                                               tAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCt                                  <  2:1933183/150‑1 (MQ=255)

                                                                                                                aGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTa                                 <  1:1174569/150‑1 (MQ=255)

                                                                                                                      tATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCCAAACCGATCCATTGACGCATCACTGGGGATAAATCGAAGTTACGCATAATAATTTCTCCATCTAAGAAGc                           >  2:3832592/1‑150 (MQ=255)

                                                                                                                       aTCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCg                          <  1:649176/150‑1 (MQ=255)

                                                                                                                         cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTTCGTTGGCCAGTTTTTAAAAACCGATCCAATGACGCAACAGTGGGGATAAATAGAAGTTAACCAAATTCAATTATCCATCAAAGAAGCGag                        >  2:39993/1‑150 (MQ=255)

                                                                                                                         cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGag                        <  2:1803278/150‑1 (MQ=255)

                                                                                                                         cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAATGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCATCTAAGAAGCGag                        >  2:2585054/1‑150 (MQ=255)

                                                                                                                           tcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagta                      <  2:324674/150‑1 (MQ=255)

                                                                                                                            cgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaa                     <  2:1221859/150‑1 (MQ=255)

                                                                                                                             gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTAATTTATCCTTCTAAGAAGCGagtaag                    >  1:4915916/1‑150 (MQ=255)

                                                                                                                              cTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagt                   >  1:241192/1‑150 (MQ=255)

                                                                                                                               ttttCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagta                  <  1:3300116/150‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt               <  2:2519807/150‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt               <  2:1335055/150‑1 (MQ=255)

                                                                                                                                   ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg              <  1:2701396/150‑1 (MQ=255)

                                                                                                                                    tcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGc             <  1:2014652/150‑1 (MQ=255)

                                                                                                                                     cAATGTTGTCCGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTGGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCa            <  2:3813753/150‑1 (MQ=255)

                                                                                                                                      aaTGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCaa           <  1:4085908/150‑1 (MQ=255)

                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc       <  1:3064147/150‑1 (MQ=255)

                                                                                                                                          ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc       <  1:3546211/150‑1 (MQ=255)

                                                                                                                                           tgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCg      >  1:862970/1‑150 (MQ=255)

                                                                                                                                           tgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCg      <  1:2222187/150‑1 (MQ=255)

                                                                                                                                            gtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGa     <  2:4469808/150‑1 (MQ=255)

                                                                                                                                             tACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGaa    >  1:3457156/1‑150 (MQ=255)

                                                                                                                                               cggaggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGa  >  2:3525947/1‑150 (MQ=255)

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGA  >  CP009273/3860013‑3860305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`