Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,907,559 A→T 20.0% P334P (CCT→CCA)  glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,907,5590AT20.0% 645.5 / 150.7 254P334P (CCT→CCA) glmUfused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand):  ref base A (98/105);  new base T (20/31);  total (118/136)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

GTAATGGTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGT  >  CP009273/3907410‑3907708
                                                                                                                                                     |                                                                                                                                                     
gTAATGGTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCACCTCAGCACCa                                                                                                                                                       >  1:3817086/1‑150 (MQ=255)
  aaTGGTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTgg                                                                                                                                                     <  1:1653996/150‑1 (MQ=255)
    tGGTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGAc                                                                                                                                                   <  1:1118888/150‑1 (MQ=255)
     ggTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACg                                                                                                                                                  <  1:2858566/150‑1 (MQ=255)
      gTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACgc                                                                                                                                                 >  2:2470286/1‑150 (MQ=255)
          ccGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACgcaaac                                                                                                                                             <  2:1157688/150‑1 (MQ=255)
               ccGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACgcaaacgggca                                                                                                                                        <  1:788246/150‑1 (MQ=255)
                 gATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACgcaaacgggcaaa                                                                                                                                      <  1:2680217/150‑1 (MQ=255)
                   tGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACgcaaacgggcaaacg                                                                                                                                    >  2:5063051/1‑150 (MQ=255)
                      tAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCaaacgggcaaacgggc                                                                                                                                 <  2:4957562/150‑1 (MQ=255)
                       aaCGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGcc                                                                                                                                >  2:1630926/1‑150 (MQ=255)
                        aCGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCACACGGGCAAACGTGCCa                                                                                                                               >  2:30070/1‑150 (MQ=255)
                          gTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAAt                                                                                                                             >  1:4450339/1‑150 (MQ=255)
                          gTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAAt                                                                                                                             >  1:5043167/1‑150 (MQ=255)
                                  cAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAgg                                                                                                                     <  2:1379382/150‑1 (MQ=255)
                                  cAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAgg                                                                                                                     >  1:1777919/1‑150 (MQ=255)
                                    aTTTCCGCATAGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGcc                                                                                                                   <  2:1724499/150‑1 (MQ=255)
                                      ttCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGc                                                                                                                 >  1:2123670/1‑150 (MQ=255)
                                          gCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGcc                                                                                                             <  2:3673205/150‑1 (MQ=255)
                                          gCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGcc                                                                                                             <  1:561719/150‑1 (MQ=255)
                                           cATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCa                                                                                                            >  2:4329219/1‑150 (MQ=255)
                                           cATCGCCCAGGTAAGTCAGAAGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCa                                                                                                            <  2:3993916/150‑1 (MQ=255)
                                            aTCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCACACGGGCCAATGGTACAGGCCGCTGCCAg                                                                                                           >  2:4664506/1‑150 (MQ=255)
                                            aTCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAg                                                                                                           >  1:3099933/1‑150 (MQ=255)
                                            aTCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAg                                                                                                           >  2:481372/1‑150 (MQ=255)
                                             tCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGa                                                                                                          >  2:3159088/1‑150 (MQ=255)
                                              cGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGAt                                                                                                         <  2:610690/150‑1 (MQ=255)
                                               gCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGAtt                                                                                                        <  2:1513633/150‑1 (MQ=255)
                                                cccAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTc                                                                                                       >  2:3297686/1‑150 (MQ=255)
                                                 ccAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCg                                                                                                      >  1:3298888/1‑150 (MQ=255)
                                                 ccAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCg                                                                                                      >  1:3298797/1‑150 (MQ=255)
                                                  cAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGc                                                                                                     >  1:3266102/1‑150 (MQ=255)
                                                   aGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCa                                                                                                    <  2:2198020/150‑1 (MQ=255)
                                                    ggTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCAt                                                                                                   >  2:2817362/1‑150 (MQ=255)
                                                      tAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCt                                                                                                 >  1:4328013/1‑150 (MQ=255)
                                                         gTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTcc                                                                                              >  2:2038191/1‑150 (MQ=255)
                                                         gTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTcc                                                                                              <  1:3090508/150‑1 (MQ=255)
                                                           cAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCcac                                                                                            >  2:4376566/1‑150 (MQ=255)
                                                              aTGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAAc                                                                                         >  2:2922958/1‑150 (MQ=255)
                                                              aTGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAAc                                                                                         <  2:2664531/150‑1 (MQ=255)
                                                                 aCCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGt                                                                                      <  2:4075805/150‑1 (MQ=255)
                                                                 aCCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGt                                                                                      <  2:1482422/150‑1 (MQ=255)
                                                                 aCCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGACAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGt                                                                                      <  2:2225746/150‑1 (MQ=255)
                                                                   cAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGtat                                                                                    >  1:195297/1‑150 (MQ=255)
                                                                     gCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATAc                                                                                  <  1:4344680/150‑1 (MQ=255)
                                                                      cTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACg                                                                                 >  2:2579442/1‑150 (MQ=255)
                                                                      cTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACg                                                                                 >  2:330351/1‑150 (MQ=255)
                                                                            aGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGa                                                                           <  2:4215990/150‑1 (MQ=255)
                                                                             gCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGAt                                                                          >  1:4219535/1‑150 (MQ=255)
                                                                              ccTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGAtt                                                                         <  1:2300939/150‑1 (MQ=255)
                                                                               cTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGAttt                                                                        <  1:2608600/150‑1 (MQ=255)
                                                                                tttACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTc                                                                       <  2:2424647/150‑1 (MQ=255)
                                                                                    cccAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCaa                                                                   >  2:1836288/1‑150 (MQ=255)
                                                                                    cccAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCaa                                                                   >  2:3912547/1‑150 (MQ=255)
                                                                                         aCGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAatcatc                                                              >  2:389567/1‑150 (MQ=255)
                                                                                          cgcgCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCg                                                             >  2:39781/1‑150 (MQ=255)
                                                                                            cgcTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGcc                                                           >  2:3382142/1‑150 (MQ=255)
                                                                                             gcTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCa                                                          <  2:4960980/150‑1 (MQ=255)
                                                                                               ttttttCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAAt                                                        >  2:5111605/1‑150 (MQ=255)
                                                                                                        ctcAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGtt                                               <  2:5113069/150‑1 (MQ=255)
                                                                                                         tcAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCCGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGttt                                              >  1:230609/1‑150 (MQ=255)
                                                                                                           aaCGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGttttt                                            >  1:3628560/1‑150 (MQ=255)
                                                                                                           aaCGAAGTTACCGACGTGAGCACATACCAGCAACTCAGAACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCAGCTGCCAGATTCGCAACTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGttttt                                            >  2:774770/1‑150 (MQ=255)
                                                                                                            aCGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTa                                           >  1:1384192/1‑150 (MQ=255)
                                                                                                             cGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTaa                                          <  1:1891452/150‑1 (MQ=255)
                                                                                                               aaGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATc                                        <  1:4495867/150‑1 (MQ=255)
                                                                                                                aGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTGTTAATCa                                       >  2:5127568/1‑150 (MQ=255)
                                                                                                                 gTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCAc                                      <  2:747744/150‑1 (MQ=255)
                                                                                                                  ttACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCACTCACGCTGTTTTTAATCACg                                     >  2:4326950/1‑150 (MQ=255)
                                                                                                                   tACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGc                                    >  1:117113/1‑150 (MQ=255)
                                                                                                                      cGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAAc                                 <  1:3018919/150‑1 (MQ=255)
                                                                                                                      cGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAAc                                 >  2:1300989/1‑150 (MQ=255)
                                                                                                                       gACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAAcc                                <  1:883193/150‑1 (MQ=255)
                                                                                                                       gACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAAcc                                >  2:4425291/1‑150 (MQ=255)
                                                                                                                       gACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAAcc                                >  2:3708176/1‑150 (MQ=255)
                                                                                                                        aCGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCg                               <  1:2064367/150‑1 (MQ=255)
                                                                                                                          gTGAGCACCTTCCAGCAACTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGt                             <  1:2365010/150‑1 (MQ=255)
                                                                                                                          gTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGt                             <  1:4423496/150‑1 (MQ=255)
                                                                                                                          gTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGt                             >  1:921752/1‑150 (MQ=255)
                                                                                                                           tGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTg                            <  2:4842801/150‑1 (MQ=255)
                                                                                                                            gAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGc                           <  1:2470037/150‑1 (MQ=255)
                                                                                                                            gAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGc                           <  1:3896544/150‑1 (MQ=255)
                                                                                                                               cACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCaa                        >  1:1834289/1‑150 (MQ=255)
                                                                                                                               cACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCaa                        >  1:1834396/1‑150 (MQ=255)
                                                                                                                                 ccTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAAtt                      <  1:3177082/150‑1 (MQ=255)
                                                                                                                                  cTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAAttt                     <  2:2794399/150‑1 (MQ=255)
                                                                                                                                   ttCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAAtttt                    >  2:4186884/1‑150 (MQ=255)
                                                                                                                                    tCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTc                   <  2:3542235/150‑1 (MQ=255)
                                                                                                                                      cAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCAc                 <  2:4328013/150‑1 (MQ=255)
                                                                                                                                      cAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCAc                 <  1:4052148/150‑1 (MQ=255)
                                                                                                                                      cAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCAc                 <  2:4253100/150‑1 (MQ=255)
                                                                                                                                           aCTCAGCACCTGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGAt            <  1:599940/150‑1 (MQ=255)
                                                                                                                                            cTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATg           >  1:3860345/1‑150 (MQ=255)
                                                                                                                                            cTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATg           >  1:227016/1‑150 (MQ=255)
                                                                                                                                             tCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGa          <  1:383571/150‑1 (MQ=255)
                                                                                                                                             tCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGa          >  1:1343985/1‑150 (MQ=255)
                                                                                                                                                  aCCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCgag     >  1:1071652/1‑150 (MQ=255)
                                                                                                                                                     aGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGt  >  2:1689967/1‑150 (MQ=255)
                                                                                                                                                     aGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGt  >  2:3483929/1‑150 (MQ=255)
                                                                                                                                                     aGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGt  >  2:3274714/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAATGGTTCCCGCGCCGATGTTAACGTTATCGCCAATTTCCGCATCGCCCAGGTAAGTCAGATGACCAGCTTTCGAGCCTTTACCCAGACGCGCTTTTTTCATCTCAACGAAGTTACCGACGTGAGCACCTTCCAGCAACTCAGCACCAGGACGCAAACGGGCAAACGGGCCAATGGTACAGGCCGCTGCCAGATTCGCATCTTCCACAACGGTATACGGACTGATTTCGCAATCATCGCCAATCACGCTGTTTTTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGT  >  CP009273/3907410‑3907708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑