Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,076 A→T 74.8% L114* (TTA→TAA)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,0760AT74.8% 513.1 / 193.8 242L114* (TTA→TAA) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (34/27);  new base T (107/74);  total (141/101)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

ATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTG  >  CP009273/3993929‑3994225
                                                                                                                                                   |                                                                                                                                                     
aTCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTAc                                                                                                                                                     >  1:4223556/1‑150 (MQ=255)
aTCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAc                                                                                                                                                     <  1:3705559/150‑1 (MQ=255)
   aaCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGcc                                                                                                                                                  >  1:3187995/1‑150 (MQ=255)
   aaCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGcc                                                                                                                                                  <  2:2180191/150‑1 (MQ=255)
   aaCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGcc                                                                                                                                                  <  1:1951604/150‑1 (MQ=255)
     cATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCAc                                                                                                                                                >  2:4098195/1‑150 (MQ=255)
      aTTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGACGCGAGATTTTACGCCAcc                                                                                                                                               >  1:1427133/1‑150 (MQ=255)
         ggCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTc                                                                                                                                            >  1:1428638/1‑150 (MQ=255)
          gCCCTGACTGCACCGCAAACTGACTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACTATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTct                                                                                                                                           <  1:3208489/150‑1 (MQ=255)
           cccTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctg                                                                                                                                          >  1:2093883/1‑150 (MQ=255)
           cccTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctg                                                                                                                                          >  1:4205840/1‑150 (MQ=255)
           cccTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTctg                                                                                                                                          <  2:2482358/150‑1 (MQ=255)
               gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGa                                                                                                                                      >  1:5123466/1‑150 (MQ=255)
                    cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtt                                                                                                                                 >  1:927958/1‑150 (MQ=255)
                      ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGtttt                                                                                                                               >  1:518900/1‑150 (MQ=255)
                        gCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCg                                                                                                                             >  1:2542958/1‑150 (MQ=255)
                           aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATg                                                                                                                          >  1:164720/1‑150 (MQ=255)
                           aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATg                                                                                                                          <  2:93167/150‑1 (MQ=255)
                             cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTa                                                                                                                        <  2:2112229/150‑1 (MQ=255)
                             cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTa                                                                                                                        <  1:1800269/150‑1 (MQ=255)
                               gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACa                                                                                                                      <  2:1408942/150‑1 (MQ=255)
                               gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACa                                                                                                                      <  1:2299877/150‑1 (MQ=255)
                                aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                                                                                                                     <  1:4693077/150‑1 (MQ=255)
                                  tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc                                                                                                                   <  2:3955671/150‑1 (MQ=255)
                                    tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg                                                                                                                 <  2:2868103/150‑1 (MQ=255)
                                     ctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGa                                                                                                                >  2:1963939/1‑150 (MQ=255)
                                      tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                                                                                                               >  1:187422/1‑150 (MQ=255)
                                        tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                             >  2:2529878/1‑150 (MQ=255)
                                        tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                             >  2:3414045/1‑150 (MQ=255)
                                        tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                             >  1:1489192/1‑150 (MQ=255)
                                         aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGAAATAAAACACGCCGCCACGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg                                                                                                            <  1:4770771/150‑1 (MQ=255)
                                           tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggcg                                                                                                          >  1:307570/1‑150 (MQ=255)
                                               tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATa                                                                                                      <  1:1609461/150‑1 (MQ=255)
                                               tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATa                                                                                                      <  2:200024/150‑1 (MQ=255)
                                                ttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAg                                                                                                     >  1:2472780/1‑150 (MQ=255)
                                                ttGAAGGCATCCAGACGAACGATGCCGAATTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAg                                                                                                     <  1:964208/150‑1 (MQ=255)
                                                 tGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGc                                                                                                    >  2:4811854/1‑150 (MQ=255)
                                                   aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCtt                                                                                                  >  2:2274563/1‑150 (MQ=255)
                                                   aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCtt                                                                                                  <  1:2724276/150‑1 (MQ=255)
                                                     ggCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtc                                                                                                >  1:4815621/1‑150 (MQ=255)
                                                        aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatc                                                                                             >  2:1292622/1‑150 (MQ=255)
                                                         tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatca                                                                                            >  2:5043070/1‑150 (MQ=255)
                                                         tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatca                                                                                            >  2:1628379/1‑150 (MQ=255)
                                                         tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatca                                                                                            >  2:818185/1‑150 (MQ=255)
                                                         tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatca                                                                                            <  1:803464/150‑1 (MQ=255)
                                                          ccAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTcatcat                                                                                           >  2:2999111/1‑150 (MQ=255)
                                                            aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAg                                                                                         <  2:4473997/150‑1 (MQ=255)
                                                              acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGAt                                                                                       <  2:5071846/150‑1 (MQ=255)
                                                                gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                     <  1:1117490/150‑1 (MQ=255)
                                                                gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                     >  1:4086297/1‑150 (MQ=255)
                                                                 aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgcc                                                                                    <  1:5039720/150‑1 (MQ=255)
                                                                 aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgcc                                                                                    <  1:1768277/150‑1 (MQ=255)
                                                                  acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTACATCATAGATgccg                                                                                   >  2:4543647/1‑150 (MQ=255)
                                                                   cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccgc                                                                                  >  2:1969850/1‑150 (MQ=255)
                                                                   cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCATCTGCTGACCGTGTTCGATGTACAAACCGAGCGGCGCATAGCTTACATCATAGATgccgc                                                                                  >  2:3043938/1‑150 (MQ=255)
                                                                    gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccgcc                                                                                 >  1:2353935/1‑150 (MQ=255)
                                                                    gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccgcc                                                                                 >  2:718277/1‑150 (MQ=255)
                                                                      tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGt                                                                               <  1:455024/150‑1 (MQ=255)
                                                                           aCTTTATCTCCCTCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTCAAGCGAGATTTAACGCCACCTTCAGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATc                                                                          >  2:1268745/1‑150 (MQ=255)
                                                                           aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATc                                                                          >  2:3745989/1‑150 (MQ=255)
                                                                            cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCg                                                                         >  2:2236041/1‑150 (MQ=255)
                                                                                    cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGc                                                                 <  2:2726874/150‑1 (MQ=255)
                                                                                       gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcc                                                              <  1:2081893/150‑1 (MQ=255)
                                                                                        cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcct                                                             >  2:1898224/1‑150 (MQ=255)
                                                                                        cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcct                                                             >  2:4526367/1‑150 (MQ=255)
                                                                                        cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcct                                                             <  2:2353935/150‑1 (MQ=255)
                                                                                          aCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACATAGCTGCGCATAGCTTTAATCATAGATGCCTCCGTTCATCGCCATCTGAAcctga                                                           >  1:1412886/1‑150 (MQ=255)
                                                                                           cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgac                                                          >  1:3088642/1‑150 (MQ=255)
                                                                                            aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgacc                                                         >  2:2035095/1‑150 (MQ=255)
                                                                                                aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctga                                                     >  2:2881932/1‑150 (MQ=255)
                                                                                                aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctga                                                     >  2:5045822/1‑150 (MQ=255)
                                                                                                        gccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAat                                             <  2:1855502/150‑1 (MQ=255)
                                                                                                          cAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAatat                                           >  2:3999539/1‑150 (MQ=255)
                                                                                                            ggACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATcc                                         >  2:1576868/1‑150 (MQ=255)
                                                                                                             gACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCg                                        <  2:4896006/150‑1 (MQ=255)
                                                                                                                ggATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCca                                     >  2:312913/1‑150 (MQ=255)
                                                                                                                  aTAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagc                                   <  1:2189287/150‑1 (MQ=255)
                                                                                                                      aGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCagag                               <  1:297759/150‑1 (MQ=255)
                                                                                                                       gAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGc                              >  2:3147403/1‑150 (MQ=255)
                                                                                                                        aaaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCa                             >  2:5154176/1‑150 (MQ=255)
                                                                                                                         aaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCAt                            >  1:1254604/1‑150 (MQ=255)
                                                                                                                         aaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCAt                            <  1:1665885/150‑1 (MQ=255)
                                                                                                                            tCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTa                         <  1:600403/150‑1 (MQ=255)
                                                                                                                             cccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCCGCAGAGCATGTaa                        >  2:2369970/1‑150 (MQ=255)
                                                                                                                             cccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTaa                        >  2:2369394/1‑150 (MQ=255)
                                                                                                                               cTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACg                      >  2:5054990/1‑150 (MQ=255)
                                                                                                                                   aCCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcc                  <  1:705920/150‑1 (MQ=255)
                                                                                                                                    ccTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTccc                 <  2:4379283/150‑1 (MQ=255)
                                                                                                                                     cTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcccc                <  2:4563641/150‑1 (MQ=255)
                                                                                                                                      tGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAAGATCCGCCAGCAGAGCATGTAACGTTCCCCa               <  2:59839/150‑1 (MQ=255)
                                                                                                                                       gAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCAc              >  1:2858524/1‑150 (MQ=255)
                                                                                                                                       gAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCAc              >  1:4865664/1‑150 (MQ=255)
                                                                                                                                          gCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCt           <  1:2856483/150‑1 (MQ=255)
                                                                                                                                           cGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCtt          <  2:3633288/150‑1 (MQ=255)
                                                                                                                                           cGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCtt          <  1:1045537/150‑1 (MQ=255)
                                                                                                                                            gagaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTc         >  2:4155505/1‑150 (MQ=255)
                                                                                                                                            gagaTTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTc         <  1:3573058/150‑1 (MQ=255)
                                                                                                                                               aTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGcc      <  1:2141307/150‑1 (MQ=255)
                                                                                                                                               aTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGcc      >  1:2454738/1‑150 (MQ=255)
                                                                                                                                                 ttAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCAt    <  1:2162791/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCCATTG  >  CP009273/3993929‑3994225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑