Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,068,208 C→A 100% G60G (GGC→GGA)  yihW → DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,068,2080CA95.6% 927.0 / 23.2 271G60G (GGC→GGA) yihWDeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (6/7);  new base A (150/108);  total (156/115)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

CACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGA  >  CP009273/4068062‑4068354
                                                                                                                                                  |                                                                                                                                                  
cACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGagcg                                                                                                                                                 <  2:737950/150‑1 (MQ=255)
  cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCggg                                                                                                                                               >  2:646711/1‑150 (MQ=255)
  cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCggg                                                                                                                                               >  2:448051/1‑150 (MQ=255)
  cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCggg                                                                                                                                               >  2:3691454/1‑150 (MQ=255)
   gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGt                                                                                                                                              >  1:2509038/1‑150 (MQ=255)
   gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGt                                                                                                                                              >  1:2507581/1‑150 (MQ=255)
    aCCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTc                                                                                                                                             <  2:3925920/150‑1 (MQ=255)
      cAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCgg                                                                                                                                           >  2:1106681/1‑150 (MQ=255)
                   tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGcgtcg                                                                                                                              >  1:891854/1‑150 (MQ=255)
                   tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtcg                                                                                                                              >  1:4691111/1‑150 (MQ=255)
                   tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtcg                                                                                                                              >  1:247401/1‑150 (MQ=255)
                      tCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTa                                                                                                                           >  1:2082283/1‑150 (MQ=255)
                         ccGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATa                                                                                                                        <  1:2248555/150‑1 (MQ=255)
                         ccGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATa                                                                                                                        >  2:198611/1‑150 (MQ=255)
                          cGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATAc                                                                                                                       <  1:1820113/150‑1 (MQ=255)
                            aGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACgg                                                                                                                     >  1:1223130/1‑150 (MQ=255)
                              cGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCTCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCg                                                                                                                   <  1:2817503/150‑1 (MQ=255)
                               gTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGt                                                                                                                  >  2:1520456/1‑150 (MQ=255)
                                tGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGtt                                                                                                                 <  1:1572657/150‑1 (MQ=255)
                                 gggTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTc                                                                                                                <  2:3919755/150‑1 (MQ=255)
                                   gTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGa                                                                                                              <  2:2363280/150‑1 (MQ=255)
                                     atatGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagc                                                                                                            >  2:3410865/1‑150 (MQ=255)
                                      tatGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagca                                                                                                           >  2:737653/1‑150 (MQ=255)
                                          aaTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                       >  2:229483/1‑150 (MQ=255)
                                          aaTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                       <  2:82794/150‑1 (MQ=255)
                                          aaTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                       >  1:1263846/1‑150 (MQ=255)
                                           atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTg                                                                                                      >  2:1964207/1‑150 (MQ=255)
                                           atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTg                                                                                                      >  1:4490801/1‑150 (MQ=255)
                                             atTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAg                                                                                                    >  1:2895456/1‑150 (MQ=255)
                                              ttGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAgg                                                                                                   <  1:3409931/150‑1 (MQ=255)
                                              ttGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAgg                                                                                                   >  2:738723/1‑150 (MQ=255)
                                               tgatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGGGAGGt                                                                                                  >  1:1339104/1‑150 (MQ=255)
                                               tgatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGt                                                                                                  >  2:3671666/1‑150 (MQ=255)
                                                 atgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGttt                                                                                                <  2:2196943/150‑1 (MQ=255)
                                                    aGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGc                                                                                             >  1:3169243/1‑150 (MQ=255)
                                                      cTGGCAAATCTGCTGGATGTCTCCCCGCAGACGGTCCGCCGGGATATTCGTAAATTACGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGGCGTTAATACGGCGGTCGAGCAGCGTGAGGTTTCGCac                                                                                           >  2:2851711/1‑149 (MQ=255)
                                                          cAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCg                                                                                       <  2:1366512/150‑1 (MQ=255)
                                                          cAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCg                                                                                       <  2:1254970/150‑1 (MQ=255)
                                                          cAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCg                                                                                       <  2:1254727/150‑1 (MQ=255)
                                                           aaaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGa                                                                                      >  2:786641/1‑150 (MQ=255)
                                                           aaaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGa                                                                                      >  2:3774969/1‑150 (MQ=255)
                                                           aaaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGa                                                                                      >  2:317746/1‑150 (MQ=255)
                                                           aaaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGa                                                                                      >  2:3391041/1‑150 (MQ=255)
                                                             aTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAgg                                                                                    >  1:4373228/1‑150 (MQ=255)
                                                              tCTGCTGGTTGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGTGGa                                                                                   <  1:4051991/150‑1 (MQ=255)
                                                                tgctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaa                                                                                 <  1:4282880/150‑1 (MQ=255)
                                                                  ctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCATAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaa                                                                               >  1:5005712/1‑150 (MQ=255)
                                                                   tgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaa                                                                              >  1:3290258/1‑150 (MQ=255)
                                                                     gATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaaaa                                                                            <  2:187105/150‑1 (MQ=255)
                                                                       tGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGc                                                                          >  1:2434233/1‑150 (MQ=255)
                                                                          ctcCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAt                                                                       <  1:3885089/150‑1 (MQ=255)
                                                                           tcCACGCAGACGGTCCGCCGGGATATTCGTAATTTAAGCGAGCAAGGCCTGATTACGCGCCATCCCGGTGGAGCGGGTCGGGCTTCCAGCGTCTTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAtt                                                                      <  1:538793/150‑1 (MQ=255)
                                                                           tcCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAtt                                                                      <  1:5190623/150‑1 (MQ=255)
                                                                               cGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGGGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGTGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCg                                                                  <  1:4543153/150‑1 (MQ=255)
                                                                                 cAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCCCGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGaa                                                                <  1:4415286/150‑1 (MQ=255)
                                                                                   gACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGc                                                              <  2:2692980/150‑1 (MQ=255)
                                                                                   gACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGc                                                              <  2:2802994/150‑1 (MQ=255)
                                                                                       gTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGTAAAAAAAAGCGATTGCCGAAGCGGTg                                                          <  1:1313877/150‑1 (MQ=255)
                                                                                       gTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTg                                                          >  1:1405212/1‑150 (MQ=255)
                                                                                       gTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTg                                                          <  2:1165840/150‑1 (MQ=255)
                                                                                         ccgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGc                                                        <  2:165957/150‑1 (MQ=255)
                                                                                          cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                       >  1:4228128/1‑150 (MQ=255)
                                                                                             cgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGAc                                                    >  2:201673/1‑150 (MQ=255)
                                                                                              gggATATTCGTAAATTAAGCGAGCCAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGCGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACt                                                   <  2:409034/150‑1 (MQ=255)
                                                                                              gggATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACt                                                   >  2:490385/1‑150 (MQ=255)
                                                                                              gggATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACt                                                   >  2:3099573/1‑150 (MQ=255)
                                                                                               ggATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGTAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACta                                                  <  1:2547999/150‑1 (MQ=255)
                                                                                                gATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtat                                                 >  2:4819553/1‑150 (MQ=255)
                                                                                                 atatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtata                                                >  2:176634/1‑150 (MQ=255)
                                                                                                  tatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGTGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTatat                                               >  2:4341871/1‑150 (MQ=255)
                                                                                                   atTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATAtt                                              >  1:2993509/1‑150 (MQ=255)
                                                                                                   atTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATAtt                                              <  2:3198216/150‑1 (MQ=255)
                                                                                                    ttCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTc                                             >  1:4620776/1‑150 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                           >  2:1587489/1‑150 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                           >  2:467656/1‑150 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                           <  2:703843/150‑1 (MQ=255)
                                                                                                           aTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATgg                                      >  2:4168934/1‑150 (MQ=255)
                                                                                                              aaGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTc                                   >  1:1111165/1‑150 (MQ=255)
                                                                                                               aGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTca                                  <  2:1454327/150‑1 (MQ=255)
                                                                                                               aGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTca                                  <  1:4071815/150‑1 (MQ=255)
                                                                                                                gCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaa                                 <  1:3347343/150‑1 (MQ=255)
                                                                                                                    gCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAat                             <  2:4734328/150‑1 (MQ=255)
                                                                                                                    gCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAat                             <  2:284211/150‑1 (MQ=255)
                                                                                                                       aGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATAtt                          >  1:1653897/1‑150 (MQ=255)
                                                                                                                       aGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATAtt                          >  2:2706723/1‑150 (MQ=255)
                                                                                                                          ccTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTAt                       >  2:1204549/1‑150 (MQ=255)
                                                                                                                           cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                      <  1:3741231/150‑1 (MQ=255)
                                                                                                                           cTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                      >  1:1898760/1‑150 (MQ=255)
                                                                                                                            tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGTGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                     >  2:1383020/1‑150 (MQ=255)
                                                                                                                            tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                     >  1:287934/1‑150 (MQ=255)
                                                                                                                            tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                     >  2:2138911/1‑150 (MQ=255)
                                                                                                                               ttACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCa                  >  1:3780947/1‑150 (MQ=255)
                                                                                                                               ttACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGGGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCa                  >  2:2067062/1‑150 (MQ=255)
                                                                                                                                    cgcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGt             <  2:5097047/150‑1 (MQ=255)
                                                                                                                                     gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa            >  2:3312338/1‑150 (MQ=255)
                                                                                                                                     gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa            >  2:1549639/1‑150 (MQ=255)
                                                                                                                                     gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa            <  1:801091/150‑1 (MQ=255)
                                                                                                                                       cATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACg          >  1:678650/1‑150 (MQ=255)
                                                                                                                                        aTCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGa         >  2:5169858/1‑150 (MQ=255)
                                                                                                                                         tCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGAc        >  1:2729417/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGA  >  CP009273/4068062‑4068354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑