Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,187,518 G→T 20.1% D230Y (GAT→TAT)  nudC → NADH pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,187,5180GT20.1% 669.0 / 136.9 253D230Y (GAT→TAT) nudCNADH pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (103/99);  new base T (22/29);  total (125/128)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 258 total aligned reads displayed.

GAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGT  >  CP009273/4187369‑4187667
                                                                                                                                                     |                                                                                                                                                     
 aaTTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGa                                                                                                                                                      >  1:4668542/1‑150 (MQ=255)
    tAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTAttt                                                                                                                                                   >  2:3143222/1‑150 (MQ=255)
     aaaGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTCTGACGATTTg                                                                                                                                                  >  2:2083225/1‑150 (MQ=255)
      aaGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGc                                                                                                                                                 >  1:4364822/1‑150 (MQ=255)
       aGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGcc                                                                                                                                                <  2:1100248/150‑1 (MQ=255)
       aGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGcc                                                                                                                                                >  1:4131323/1‑150 (MQ=255)
        gTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCg                                                                                                                                               >  1:695526/1‑150 (MQ=255)
        gTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCg                                                                                                                                               <  1:2831564/150‑1 (MQ=255)
          tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGtt                                                                                                                                             <  1:1806465/150‑1 (MQ=255)
          tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGtt                                                                                                                                             >  2:2762118/1‑150 (MQ=255)
          tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGtt                                                                                                                                             <  2:2007588/150‑1 (MQ=255)
           aaaaaCTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTa                                                                                                                                            <  2:3642929/150‑1 (MQ=255)
              aaCTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTc                                                                                                                                         >  1:2407205/1‑150 (MQ=255)
               aCTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTcc                                                                                                                                        <  1:1101106/150‑1 (MQ=255)
               aCTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTcc                                                                                                                                        <  1:3464956/150‑1 (MQ=255)
                cTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTccc                                                                                                                                       <  1:1685090/150‑1 (MQ=255)
                  tGCGTTACGTGACTTCTCAGCCGTGGCCGTGTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCccgc                                                                                                                                     <  1:1994970/150‑1 (MQ=255)
                     gTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCcgccgc                                                                                                                                  >  2:451900/1‑150 (MQ=255)
                       tACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGccc                                                                                                                                <  1:4128006/150‑1 (MQ=255)
                        aCGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCg                                                                                                                               >  1:80187/1‑150 (MQ=255)
                          gTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGc                                                                                                                             >  2:1624553/1‑150 (MQ=255)
                          gTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGc                                                                                                                             <  1:3360748/150‑1 (MQ=255)
                          gTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGc                                                                                                                             >  1:3743503/1‑150 (MQ=255)
                             aCTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCAcc                                                                                                                          <  1:640831/150‑1 (MQ=255)
                                 ctcAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAg                                                                                                                      >  1:47614/1‑150 (MQ=255)
                                  tcAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTGATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAgc                                                                                                                     <  2:2917946/150‑1 (MQ=255)
                                    aGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAgcgc                                                                                                                   >  2:1229992/1‑150 (MQ=255)
                                      ccGTGGCCGTTTCCTCAGTCTTAAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAgcgcgc                                                                                                                 >  2:32427/1‑150 (MQ=255)
                                            ccGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTg                                                                                                           >  2:904728/1‑150 (MQ=255)
                                                ttCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATag                                                                                                       >  2:5069948/1‑150 (MQ=255)
                                                ttCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATag                                                                                                       >  2:2490591/1‑150 (MQ=255)
                                                  ccTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATagaa                                                                                                     >  2:121989/1‑150 (MQ=255)
                                                    tCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATagaaga                                                                                                   >  2:1750047/1‑150 (MQ=255)
                                                      aGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATa                                                                                                 <  1:4924660/150‑1 (MQ=255)
                                                      aGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATa                                                                                                 <  1:4924821/150‑1 (MQ=255)
                                                       gTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATAc                                                                                                <  2:598024/150‑1 (MQ=255)
                                                        tCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACg                                                                                               >  2:4083900/1‑150 (MQ=255)
                                                         cTTTAGTGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACgg                                                                                              <  2:569157/150‑1 (MQ=255)
                                                         cTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACgg                                                                                              >  2:3325776/1‑150 (MQ=255)
                                                            tAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTgg                                                                                           >  2:690131/1‑150 (MQ=255)
                                                             actGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGc                                                                                          >  1:2966888/3‑150 (MQ=255)
                                                               tGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCATTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGa                                                                                        <  2:2010623/150‑1 (MQ=255)
                                                                gACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAt                                                                                       <  1:1330974/150‑1 (MQ=255)
                                                                gACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAt                                                                                       >  2:4763500/1‑150 (MQ=255)
                                                                 aCCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAtg                                                                                      <  1:1629404/150‑1 (MQ=255)
                                                                  ccGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAtgt                                                                                     <  1:516207/150‑1 (MQ=255)
                                                                    gcgTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATgtgt                                                                                   <  1:4133323/150‑1 (MQ=255)
                                                                     cgTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTc                                                                                  <  2:2288909/150‑1 (MQ=255)
                                                                      gTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCg                                                                                 >  2:4429981/1‑150 (MQ=255)
                                                                        ttATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCggg                                                                               <  2:1302339/150‑1 (MQ=255)
                                                                           tGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCag                                                                            >  2:4315973/1‑150 (MQ=255)
                                                                             gCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCagag                                                                          <  1:3549913/150‑1 (MQ=255)
                                                                              cGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGt                                                                         <  2:1263722/150‑1 (MQ=255)
                                                                                gAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTAt                                                                       >  1:4348138/1‑150 (MQ=255)
                                                                                    atGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGt                                                                   >  1:987485/1‑150 (MQ=255)
                                                                                    atGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGt                                                                   >  2:3794347/1‑150 (MQ=255)
                                                                                     tGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGtg                                                                  <  2:4156869/150‑1 (MQ=255)
                                                                                      gACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGtga                                                                 >  1:2756841/1‑150 (MQ=255)
                                                                                       aCAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGtgat                                                                <  2:4546233/150‑1 (MQ=255)
                                                                                           cggcgACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATa                                                            <  1:2951729/150‑1 (MQ=255)
                                                                                           cggcgACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATa                                                            <  1:378257/150‑1 (MQ=255)
                                                                                             gcgACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATaca                                                          <  2:1216845/150‑1 (MQ=255)
                                                                                               gACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGGTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACt                                                        <  2:133857/150‑1 (MQ=255)
                                                                                                aCATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTg                                                       <  1:1038434/150‑1 (MQ=255)
                                                                                                     gTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGc                                                  >  2:1634229/1‑150 (MQ=255)
                                                                                                       gATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCt                                                >  2:350244/1‑150 (MQ=255)
                                                                                                         tCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGa                                              <  2:47614/150‑1 (MQ=255)
                                                                                                           gACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACg                                            >  2:2317840/1‑150 (MQ=255)
                                                                                                            aCCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGc                                           >  2:1223877/1‑150 (MQ=255)
                                                                                                            aCCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGc                                           >  1:4414356/1‑150 (MQ=255)
                                                                                                             cccGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCa                                          <  1:3183758/150‑1 (MQ=255)
                                                                                                             cccGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCa                                          >  1:363636/1‑150 (MQ=255)
                                                                                                                  aaGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAg                                     >  1:1898894/1‑150 (MQ=255)
                                                                                                                  aaGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAg                                     >  1:1898811/1‑150 (MQ=255)
                                                                                                                    gAATTGCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGa                                   <  1:4246997/150‑1 (MQ=255)
                                                                                                                     aaTTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGaa                                  <  1:4218051/150‑1 (MQ=255)
                                                                                                                         gCTCGAGGCGAACTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGc                              >  1:1426297/1‑150 (MQ=255)
                                                                                                                           tCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCa                            >  1:3791238/1‑150 (MQ=255)
                                                                                                                           tCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCa                            <  2:3310219/150‑1 (MQ=255)
                                                                                                                            cGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCaa                           <  1:4080140/150‑1 (MQ=255)
                                                                                                                            cGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCaa                           >  1:5111593/1‑150 (MQ=255)
                                                                                                                            cGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCaa                           >  2:1720064/1‑150 (MQ=255)
                                                                                                                              aGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCaaaa                         >  1:2172405/1‑150 (MQ=255)
                                                                                                                               ggCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAAt                        <  2:4131323/150‑1 (MQ=255)
                                                                                                                                 cGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGa                      >  1:1397961/1‑150 (MQ=255)
                                                                                                                                 cGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGa                      >  2:1988427/1‑150 (MQ=255)
                                                                                                                                   aaCTGGTATCGCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGAcc                    <  2:5025907/150‑1 (MQ=255)
                                                                                                                                   aaCTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGAcc                    <  2:4935320/150‑1 (MQ=255)
                                                                                                                                       ggTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAAc                <  1:123061/150‑1 (MQ=255)
                                                                                                                                            cGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTaaa           <  2:74703/150‑1 (MQ=255)
                                                                                                                                             gCTATGACTATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTaaaa          <  2:349386/150‑1 (MQ=255)
                                                                                                                                               tATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAAc        >  1:4971633/1‑150 (MQ=255)
                                                                                                                                                aTGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACg       >  2:461168/1‑150 (MQ=255)
                                                                                                                                                  gACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGAt     <  1:4297453/150‑1 (MQ=255)
                                                                                                                                                   aCGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATc    <  2:3887115/150‑1 (MQ=255)
                                                                                                                                                   aCGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATc    <  2:3887032/150‑1 (MQ=255)
                                                                                                                                                   aCGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATc    <  2:31793/150‑1 (MQ=255)
                                                                                                                                                    cGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCg   >  1:2580476/1‑150 (MQ=255)
                                                                                                                                                     gATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGt  <  1:2311658/150‑1 (MQ=255)
                                                                                                                                                     gATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGt  >  2:2075621/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGT  >  CP009273/4187369‑4187667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑