BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,211,576	G→T	67.9%	S280R (AGC→AGA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,211,576	0	G	T	67.9%	236.7 / 217.5	200	S280R (AGC→AGA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (26/38); new base T (68/68); total (94/106)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 203 total aligned reads displayed.

CTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCC  >  CP009273/4211428‑4211725

cTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGc                                                                                                                                                      >  2:2871689/1‑150 (MQ=255)

   ttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCa                                                                                                                                                   <  2:3493474/150‑1 (MQ=255)

      aCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATaa                                                                                                                                                >  1:1688356/1‑150 (MQ=255)

      aCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATaa                                                                                                                                                <  2:4701043/150‑1 (MQ=255)

       cATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAAt                                                                                                                                               <  2:3233908/150‑1 (MQ=255)

       cATAAATAGCTGTTCTTTCGGAATTGCTGTAATTATATCAACAGATAGATTAATAACATGATCATTTGACCATATCAGTAGGATATAAACTACATTCATAAAATCCAACAGAGAATAGTGTTTAAAATTCTATATGATTTCTCTaataat                                                                                                                                               >  2:2344117/1‑150 (MQ=255)

          cAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCt                                                                                                                                            >  1:2268777/1‑150 (MQ=255)

           aaTAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAAGAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCtt                                                                                                                                           >  2:825137/1‑150 (MQ=255)

             tAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAt                                                                                                                                         >  1:5071949/1‑150 (MQ=255)

              aGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAtt                                                                                                                                        >  1:3768213/1‑150 (MQ=255)

               gCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTAttt                                                                                                                                       >  2:3498936/1‑150 (MQ=255)

               gCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTAttt                                                                                                                                       <  2:1542259/150‑1 (MQ=255)

                cTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTAtttt                                                                                                                                      >  1:2257915/1‑150 (MQ=255)

                 tGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTa                                                                                                                                     >  2:3331856/1‑150 (MQ=255)

                    tCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCg                                                                                                                                  >  2:4195929/1‑150 (MQ=255)

                    tCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCg                                                                                                                                  <  2:3976613/150‑1 (MQ=255)

                        tCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttag                                                                                                                              >  2:2282506/1‑150 (MQ=255)

                         cGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttagt                                                                                                                             <  2:376995/150‑1 (MQ=255)

                         cGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttagt                                                                                                                             <  1:1155020/150‑1 (MQ=255)

                           gAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCgttagtta                                                                                                                           >  2:2226762/1‑150 (MQ=255)

                           gAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttagtta                                                                                                                           >  2:4144832/1‑150 (MQ=255)

                           gAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttagtta                                                                                                                           <  1:2513321/150‑1 (MQ=255)

                            aaGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTAc                                                                                                                          <  2:2925340/150‑1 (MQ=255)

                                      attattGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCa                                                                                                                >  2:4042872/1‑150 (MQ=255)

                                       ttattGCAACAGATTGATTAAGAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCAt                                                                                                               <  2:720703/150‑1 (MQ=255)

                                         attGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaa                                                                                                             <  1:3478016/150‑1 (MQ=255)

                                          ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATaaa                                                                                                            <  1:2021935/150‑1 (MQ=255)

                                            gCAACATATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAAcc                                                                                                          <  2:2840106/150‑1 (MQ=255)

                                            gCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAAcc                                                                                                          <  1:1817733/150‑1 (MQ=255)

                                             taaCAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGACTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCg                                                                                                         >  2:4212674/2‑150 (MQ=255)

                                             cAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCg                                                                                                         <  1:3834494/150‑1 (MQ=255)

                                                 aGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                     <  2:4981227/150‑1 (MQ=255)

                                                 aGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAAc                                                                                                     <  2:4815585/150‑1 (MQ=255)

                                                   attgattAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCa                                                                                                   <  2:1708172/150‑1 (MQ=255)

                                                   attgattAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCa                                                                                                   <  1:1705194/150‑1 (MQ=255)

                                                         taataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAAt                                                                                             <  2:1354541/150‑1 (MQ=255)

                                                         taataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAAt                                                                                             <  2:2395789/150‑1 (MQ=255)

                                                         taataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAAt                                                                                             >  1:2775266/1‑150 (MQ=255)

                                                          aataaCATGATCATTTGACCATTTCAGGAGGCTATTATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATg                                                                                            <  1:1587836/150‑1 (MQ=255)

                                                          aataaCATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATg                                                                                            >  2:214541/1‑150 (MQ=255)

                                                               cATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTAc                                                                                       <  2:4529927/150‑1 (MQ=255)

                                                                aTGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAAAATTCTCTTTGATTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACa                                                                                      >  2:1680485/1‑150 (MQ=255)

                                                                   aTCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATcc                                                                                   >  1:4173169/1‑150 (MQ=255)

                                                                    tCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCt                                                                                  <  1:2596723/150‑1 (MQ=255)

                                                                     cATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                                 <  1:3518848/150‑1 (MQ=255)

                                                                     cATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTTTTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCtt                                                                                 <  1:4351736/150‑1 (MQ=255)

                                                                      aTTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTc                                                                                >  1:4550916/1‑150 (MQ=255)

                                                                        ttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAg                                                                              >  1:4130759/1‑150 (MQ=255)

                                                                        ttGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAg                                                                              >  2:3920178/1‑150 (MQ=255)

                                                                         tGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGt                                                                             >  2:1225789/1‑150 (MQ=255)

                                                                         tGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGt                                                                             >  1:3641789/1‑150 (MQ=255)

                                                                          gACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGtt                                                                            >  1:910084/1‑150 (MQ=255)

                                                                          gACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTAAGtt                                                                            >  2:2709182/1‑150 (MQ=255)

                                                                            ccATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtgt                                                                          <  2:5072540/150‑1 (MQ=255)

                                                                            ccATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTtgt                                                                          >  1:4693863/1‑150 (MQ=255)

                                                                               tttCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGtt                                                                       <  2:376859/150‑1 (MQ=255)

                                                                               tttCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGtt                                                                       >  2:770053/1‑150 (MQ=255)

                                                                                   aggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                   >  2:120232/1‑150 (MQ=255)

                                                                                   aggaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCttt                                                                   >  1:5126372/1‑150 (MQ=255)

                                                                                    ggagtCTATAATCTACATTCATAAAATCCATCAGAGAATTGTGTTTAATATTCTCTTTGCTTTCTCTCATTATTCTTATTTTATCGTTTGTTACTTTATGATCATAATCCGAAAACACGTAATTGGTACATCCTTCAGTTGTGTTCTTTa                                                                  <  1:4449050/150‑1 (MQ=255)

                                                                                     gaggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTAt                                                                 <  1:1783993/150‑1 (MQ=255)

                                                                                       ggCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTc                                                               >  1:1290998/1‑150 (MQ=255)

                                                                                          tataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTa                                                            <  2:5162812/150‑1 (MQ=255)

                                                                                          tataATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTa                                                            <  2:57858/150‑1 (MQ=255)

                                                                                            taATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGc                                                          <  1:776946/150‑1 (MQ=255)

                                                                                             aaTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGcc                                                         >  2:858740/1‑150 (MQ=255)

                                                                                             aaTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGcc                                                         <  2:631077/150‑1 (MQ=255)

                                                                                              aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                        >  2:1080529/1‑150 (MQ=255)

                                                                                              aTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCg                                                        >  1:3296391/1‑150 (MQ=255)

                                                                                                 tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                     <  1:4229064/150‑1 (MQ=255)

                                                                                                 tACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGAc                                                     <  1:4181443/150‑1 (MQ=255)

                                                                                                            aaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCAt                                          <  1:770053/150‑1 (MQ=255)

                                                                                                             aTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATg                                         <  1:5090170/150‑1 (MQ=255)

                                                                                                              tCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATgg                                        <  2:1613532/150‑1 (MQ=255)

                                                                                                                     gagaATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcca                                 >  2:507633/1‑150 (MQ=255)

                                                                                                                     gagaATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATcca                                 >  2:3738095/1‑150 (MQ=255)

                                                                                                                       gaATAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacc                               <  1:5134978/150‑1 (MQ=255)

                                                                                                                        aaTAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacca                              >  2:3476568/1‑150 (MQ=255)

                                                                                                                        aaTAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATccacca                              >  2:3859239/1‑150 (MQ=255)

                                                                                                                          tAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGt                            >  1:4677357/1‑150 (MQ=255)

                                                                                                                          tAGTGTTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGt                            <  1:571706/150‑1 (MQ=255)

                                                                                                                            gtgtTTAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACAAGTaa                          >  2:4884495/1‑150 (MQ=255)

                                                                                                                               tttAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAatat                       <  2:11344/150‑1 (MQ=255)

                                                                                                                               tttAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAatat                       <  2:4972479/150‑1 (MQ=255)

                                                                                                                                 tAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATgg                     >  2:1724288/1‑150 (MQ=255)

                                                                                                                                 tAATATTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATgg                     >  1:5003386/1‑150 (MQ=255)

                                                                                                                                   atatTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCt                   >  1:1210544/1‑150 (MQ=255)

                                                                                                                                     atTCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGc                 >  1:215841/1‑150 (MQ=255)

                                                                                                                                      ttCTCTTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGcc                <  2:5109647/150‑1 (MQ=255)

                                                                                                                                        ctctTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCAt              <  2:1655657/150‑1 (MQ=255)

                                                                                                                                         tctTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATa             >  1:3413552/1‑150 (MQ=255)

                                                                                                                                         tctTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATa             <  1:3178392/150‑1 (MQ=255)

                                                                                                                                          ctTTGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATaa            >  2:4948051/1‑150 (MQ=255)

                                                                                                                                           tttGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAac           <  2:4775231/150‑1 (MQ=255)

                                                                                                                                            ttGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAaca          >  2:158192/1‑150 (MQ=255)

                                                                                                                                             tGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAacac         >  2:4136340/1‑150 (MQ=255)

                                                                                                                                             tGCTTTCTCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGAAATATGGCTGCCATAacac         >  2:4135537/1‑150 (MQ=255)

                                                                                                                                                tttCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGa      <  2:4656052/150‑1 (MQ=255)

                                                                                                                                                 ttCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAt     <  1:2903168/150‑1 (MQ=255)

                                                                                                                                                    gCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGcc  >  1:2350653/1‑150 (MQ=255)

CTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCC  >  CP009273/4211428‑4211725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`