BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,250,324	G→A	22.9%	A26T (GCA→ACA)	yjbL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,250,324	0	G	A	22.9%	371.0 / 111.5	188	A26T (GCA→ACA)	yjbL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (76/68); new base A (22/21); total (98/90)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 188 total aligned reads displayed.

GATTTTTGATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACGCATAATT  >  CP009273/4250177‑4250471

gATTTTTGATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACa                                                                                                                                                   <  1:4726563/150‑1 (MQ=255)

     ttGATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCaa                                                                                                                                              >  1:4882526/1‑150 (MQ=255)

     ttGATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCaa                                                                                                                                              >  1:4882507/1‑150 (MQ=255)

      tGATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCaaa                                                                                                                                             >  1:1032218/1‑150 (MQ=255)

       gATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAg                                                                                                                                            >  1:2038587/1‑150 (MQ=255)

          aGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATa                                                                                                                                         >  1:4601605/1‑150 (MQ=255)

           gcgACCAGATGCCAAAAGAATTATAAGGCCCACACGGAACGACAAAAAAGCTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATaa                                                                                                                                        <  1:3813305/148‑1 (MQ=255)

             gACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAAt                                                                                                                                      >  2:3301521/1‑150 (MQ=255)

             gACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAAt                                                                                                                                      <  1:2556865/150‑1 (MQ=255)

             gACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAAt                                                                                                                                      <  1:2141508/150‑1 (MQ=255)

               ccAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATACTACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTa                                                                                                                                    <  2:5118511/150‑1 (MQ=255)

                cAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCACTCAAAGATAAATTAg                                                                                                                                   >  2:2220714/1‑150 (MQ=255)

                 aGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGa                                                                                                                                  <  1:690364/150‑1 (MQ=255)

                   aTGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGata                                                                                                                                >  1:4700809/1‑150 (MQ=255)

                    tGGCAAAAGACTGATAAGGAGGACACGGTACGACAAACAAGGTAGATGAATCATGTTGAAGAGAATACCCGGTGCAACTGGATACTTGAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGataa                                                                                                                               <  2:2647685/150‑1 (MQ=255)

                    tGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGataa                                                                                                                               >  1:2659951/1‑150 (MQ=255)

                    tGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGataa                                                                                                                               >  2:2131534/1‑150 (MQ=255)

                      gCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGataata                                                                                                                             <  1:4330338/150‑1 (MQ=255)

                          aaGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAgg                                                                                                                         <  1:3765711/150‑1 (MQ=255)

                           aGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGt                                                                                                                        >  2:3492361/1‑150 (MQ=255)

                            gAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGtt                                                                                                                       <  2:670410/150‑1 (MQ=255)

                             aaTTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTtc                                                                                                                      <  2:4295914/150‑1 (MQ=255)

                             aaTTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTtc                                                                                                                      >  2:3230049/1‑150 (MQ=255)

                                   aaGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTtcaatcac                                                                                                                >  2:4919712/1‑149 (MQ=255)

                                   aaGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTtcaatcaa                                                                                                                >  2:1527132/1‑150 (MQ=255)

                                     ggaggaCACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAg                                                                                                              <  2:449066/150‑1 (MQ=255)

                                      gaggaCACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATCATAGAGGTTCAATCAAAgg                                                                                                             >  2:1968935/1‑150 (MQ=255)

                                      gaggaCACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAgg                                                                                                             <  1:1944930/150‑1 (MQ=255)

                                       aggaCACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGt                                                                                                            <  1:4718302/150‑1 (MQ=255)

                                        ggaCACGGTACGACAAAAAAGGTAGATGAATCATGTTGANGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGta                                                                                                           >  1:4787414/1‑150 (MQ=255)

                                          acacGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaa                                                                                                         <  1:611739/150‑1 (MQ=255)

                                          acacGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaa                                                                                                         >  1:2811368/1‑150 (MQ=255)

                                           cacGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaat                                                                                                        >  2:1410463/1‑150 (MQ=255)

                                             cGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaatta                                                                                                      <  1:3439277/150‑1 (MQ=255)

                                             cGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaatta                                                                                                      <  1:3439266/150‑1 (MQ=255)

                                              ggTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaattaa                                                                                                     <  1:1262451/150‑1 (MQ=255)

                                               gTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGtaaattaaa                                                                                                    <  1:776009/150‑1 (MQ=255)

                                                   gACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAaataat                                                                                                >  2:359513/1‑150 (MQ=255)

                                                     cAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAatat                                                                                              >  2:1651223/1‑150 (MQ=255)

                                                     cAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAatat                                                                                              >  2:3189057/1‑150 (MQ=255)

                                                     cAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAatat                                                                                              >  2:4953933/1‑150 (MQ=255)

                                                       acaaaGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATAATTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAatatat                                                                                            <  1:4783291/148‑1 (MQ=255)

                                                         aaaGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAATGGTAAATTAAATAatatatat                                                                                          >  2:3206407/1‑150 (MQ=255)

                                                          aaGGGAGAGGAATCATGTTGAAGATAATACCAGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCTATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATg                                                                                         <  2:4782445/150‑1 (MQ=255)

                                                           aGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATCAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATgg                                                                                        >  2:4415712/1‑150 (MQ=255)

                                                           aGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATgg                                                                                        >  2:1826500/1‑150 (MQ=255)

                                                            ggTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGt                                                                                       <  2:3506151/150‑1 (MQ=255)

                                                             gTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTTGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTa                                                                                      <  1:54974/150‑1 (MQ=255)

                                                             gTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTa                                                                                      <  1:1902540/150‑1 (MQ=255)

                                                                     aTCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTAtt                                                                              <  2:2893203/150‑1 (MQ=255)

                                                                       cATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGa                                                                            >  1:3837858/1‑150 (MQ=255)

                                                                        aTGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTCTTGAt                                                                           >  1:4615356/1‑150 (MQ=255)

                                                                        aTGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGAt                                                                           >  2:2782125/1‑150 (MQ=255)

                                                                        aTGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGAt                                                                           >  1:1631372/1‑150 (MQ=255)

                                                                        aTGTTGAAGATAACACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGAt                                                                           >  1:2366364/1‑150 (MQ=255)

                                                                              aaGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCa                                                                     <  2:2217563/150‑1 (MQ=255)

                                                                                gATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAgc                                                                   >  1:1303037/1‑150 (MQ=255)

                                                                                   aataCCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCt                                                                <  2:1474928/150‑1 (MQ=255)

                                                                                      accctctGCAACTGGAAACATAAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGATGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCg                                                             <  2:2647458/144‑1 (MQ=255)

                                                                                      aCCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATCGAGGTTCAATCCATGGTAAATTAAATCATATATATGGTAAATCTATTGATTATGCAGCGCTGCg                                                             >  2:3824116/1‑150 (MQ=255)

                                                                                      aCCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCg                                                             <  1:885627/150‑1 (MQ=255)

                                                                                       ccaggTGAAACTGGATACTATAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATAAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGt                                                            <  2:1332915/147‑1 (MQ=255)

                                                                                        ccGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTc                                                           >  2:1647108/1‑150 (MQ=255)

                                                                                        ccGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTc                                                           <  1:1289845/150‑1 (MQ=255)

                                                                                         cGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCa                                                          <  2:1810388/150‑1 (MQ=255)

                                                                                         cGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCa                                                          >  1:31556/1‑150 (MQ=255)

                                                                                            tGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCg                                                       >  2:1933110/1‑150 (MQ=255)

                                                                                              cAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCggg                                                     >  1:3773316/1‑150 (MQ=255)

                                                                                               aaCTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGa                                                    >  2:4805429/1‑150 (MQ=255)

                                                                                                  tGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGNTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGatat                                                 <  1:4787109/150‑1 (MQ=255)

                                                                                                   ggATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGAtata                                                <  1:3087238/150‑1 (MQ=255)

                                                                                                    gATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATaa                                               >  2:2645534/1‑150 (MQ=255)

                                                                                                      tACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAatt                                             <  2:3391710/150‑1 (MQ=255)

                                                                                                        cTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAattat                                           <  2:427193/150‑1 (MQ=255)

                                                                                                           taataaAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGc                                        >  1:4429172/1‑150 (MQ=255)

                                                                                                            aataaAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCa                                       >  1:2454365/1‑150 (MQ=255)

                                                                                                            aataaAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCa                                       >  1:920244/1‑150 (MQ=255)

                                                                                                             ataaAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCaa                                      >  2:3008184/1‑150 (MQ=255)

                                                                                                             ataaAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCaa                                      <  1:1988666/150‑1 (MQ=255)

                                                                                                               aaaaCACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCaaaa                                    <  1:4496277/150‑1 (MQ=255)

                                                                                                               aaaaCACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCaaaa                                    >  2:1289832/1‑150 (MQ=255)

                                                                                                               aaaaCACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCaaaa                                    >  2:3436260/1‑150 (MQ=255)

                                                                                                                 aaCACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAAt                                  >  1:325742/1‑150 (MQ=255)

                                                                                                                        aaTTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTg                           <  1:2196481/150‑1 (MQ=255)

                                                                                                                          ttCAAATCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGtt                         >  2:1013070/1‑150 (MQ=255)

                                                                                                                             aaaTCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTAt                      <  2:4580649/150‑1 (MQ=255)

                                                                                                                             aaaTCAATTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTAt                      <  1:822340/150‑1 (MQ=255)

                                                                                                                               aTCAATTATATAATGAATATGCAATAAAATATAAATTAGATAATAGAGGTTCAATCATAGTTAAATTAAATAATATTTATGGTAAATCTATTGATTATGCAGATCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTc                    <  1:4055904/150‑1 (MQ=255)

                                                                                                                                  aaTTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAAc                 <  2:4667425/150‑1 (MQ=255)

                                                                                                                                  aaTTCGATAATGAAGATACAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATCAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAAc                 >  2:3718852/1‑150 (MQ=255)

                                                                                                                                   aTTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACg                >  1:337384/1‑150 (MQ=255)

                                                                                                                                   aTTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGAGATAATTATTGCAAAAATAGATTTGTTTATTCAACg                >  1:3187522/1‑150 (MQ=255)

                                                                                                                                     tCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGaa              >  2:4778500/1‑150 (MQ=255)

                                                                                                                                        aTAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCa           >  1:1018340/1‑150 (MQ=255)

                                                                                                                                        aTAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCa           >  1:1822970/1‑150 (MQ=255)

                                                                                                                                          aaTGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACg         >  2:1698079/1‑150 (MQ=255)

                                                                                                                                            tGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACGCa       <  1:2313829/150‑1 (MQ=255)

                                                                                                                                               aGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACGCATaa    <  2:4925990/150‑1 (MQ=255)

                                                                                                                                               aGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACGCATaa    >  2:929635/1‑150 (MQ=255)

                                                                                                                                                 aTGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACGCATAAtt  <  1:293962/150‑1 (MQ=255)

GATTTTTGATAGTGACCAGATGGCAAAAGAATTATAAGGAGGACACGGTACGACAAAAAAGGTAGATGAATCATGTTGAAGATAATACCCGGTGCAACTGGATACTTTAATAAAACACTCAATTCAAATCAATTCGATAATGAAGATGCAATCAAAGATAAATTAGATAATAGAGGTTCAATCAAAGGTAAATTAAATAATATATATGGTAAATCTATTGATTATGCAGCGCTGCGTCATCGGGATATAATTATTGCAAAAATAGATTTGTTTATTCAACGAATCACGCATAATT  >  CP009273/4250177‑4250471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`