Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,279,051 G→T 15.0% E454* (GAG→TAG)  nrfA → nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,279,0510GT15.0% 894.8 / 107.2 285E454* (GAG→TAG) nrfAnitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base G (110/132);  new base T (26/17);  total (136/149)
Fisher's exact test for biased strand distribution p-value = 9.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

CGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGTTATTAACTG  >  CP009273/4278902‑4279200
                                                                                                                                                     |                                                                                                                                                     
cGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCg                                                                                                                                                       <  2:213848/150‑1 (MQ=255)
cGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCg                                                                                                                                                       >  1:208111/1‑150 (MQ=255)
    gATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGaa                                                                                                                                                   <  1:2016345/150‑1 (MQ=255)
       ggATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCa                                                                                                                                                >  1:1105800/1‑150 (MQ=255)
       ggATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCa                                                                                                                                                <  1:139948/150‑1 (MQ=255)
            aaGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACt                                                                                                                                           <  1:1918406/150‑1 (MQ=255)
               cggcggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTtca                                                                                                                                        <  1:1097970/150‑1 (MQ=255)
                  cggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTtcatca                                                                                                                                     >  1:4961546/1‑150 (MQ=255)
                  cggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTtcatca                                                                                                                                     <  2:3196363/150‑1 (MQ=255)
                  cggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTtcatca                                                                                                                                     <  1:2413732/150‑1 (MQ=255)
                   ggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCaa                                                                                                                                    >  1:3236058/1‑150 (MQ=255)
                    gATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCaaa                                                                                                                                   >  2:3450390/1‑150 (MQ=255)
                       gcacgcacCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACg                                                                                                                                <  2:2772699/150‑1 (MQ=255)
                        cacgcacCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACgg                                                                                                                               >  1:1160088/1‑150 (MQ=255)
                         acgcacCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGt                                                                                                                              <  2:1020736/150‑1 (MQ=255)
                           gcacCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGa                                                                                                                            <  2:1110822/150‑1 (MQ=255)
                            cacCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGAt                                                                                                                           >  1:3915504/1‑150 (MQ=255)
                               cAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATccc                                                                                                                        <  2:2186785/150‑1 (MQ=255)
                               cAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATccc                                                                                                                        <  1:278470/150‑1 (MQ=255)
                                aaaCTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCg                                                                                                                       >  1:1246652/1‑150 (MQ=255)
                                aaaCTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCg                                                                                                                       <  2:5189333/150‑1 (MQ=255)
                                aaaCTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCg                                                                                                                       >  2:4733827/1‑150 (MQ=255)
                                aaaCTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATAGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCg                                                                                                                       <  1:4478040/150‑1 (MQ=255)
                                  aCTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCa                                                                                                                     <  2:4240228/150‑1 (MQ=255)
                                    tGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAATCGGTGATCCCGCAGt                                                                                                                   >  2:4337446/1‑150 (MQ=255)
                                      gcgcgcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTgg                                                                                                                 <  1:2092746/150‑1 (MQ=255)
                                       cgcgcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTggg                                                                                                                >  1:3697967/1‑150 (MQ=255)
                                         cgcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaa                                                                                                              <  2:4182950/150‑1 (MQ=255)
                                         cgcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaa                                                                                                              <  2:3355703/150‑1 (MQ=255)
                                          gcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaag                                                                                                             <  1:215498/150‑1 (MQ=255)
                                          gcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaag                                                                                                             <  1:2829871/150‑1 (MQ=255)
                                              gctgGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACa                                                                                                         >  2:2536535/1‑150 (MQ=255)
                                               ctgGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAg                                                                                                        >  1:2043932/1‑150 (MQ=255)
                                                tgGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAgg                                                                                                       >  1:279635/1‑150 (MQ=255)
                                                 ggCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGc                                                                                                      >  1:59853/1‑150 (MQ=255)
                                                 ggCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGc                                                                                                      >  1:2369634/1‑150 (MQ=255)
                                                   cGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCGAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGGGGGAAGAACAGGCAc                                                                                                    >  1:3353154/1‑150 (MQ=255)
                                                      ccAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTa                                                                                                 >  2:1838562/1‑150 (MQ=255)
                                                        aaaGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTaaa                                                                                               >  2:5188216/1‑150 (MQ=255)
                                                          aGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTaaaaa                                                                                             >  2:5024111/1‑150 (MQ=255)
                                                                  cccATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGt                                                                                     <  2:716530/150‑1 (MQ=255)
                                                                   ccATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGtt                                                                                    >  2:2980637/1‑150 (MQ=255)
                                                                   ccATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGtt                                                                                    <  1:2534202/150‑1 (MQ=255)
                                                                        aaaTCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGcc                                                                               <  1:3769977/150‑1 (MQ=255)
                                                                         aaTCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCa                                                                              <  1:1776888/150‑1 (MQ=255)
                                                                            ccAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATa                                                                           <  1:1618628/150‑1 (MQ=255)
                                                                             cAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATaa                                                                          <  1:4652147/150‑1 (MQ=255)
                                                                             cAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATaa                                                                          <  1:400616/150‑1 (MQ=255)
                                                                             cAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATaa                                                                          >  1:5160682/1‑150 (MQ=255)
                                                                              aGATCCCGGATATCTCAACCACAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAAc                                                                         <  1:3027781/150‑1 (MQ=255)
                                                                                 tCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAAcccc                                                                      >  2:436326/1‑150 (MQ=255)
                                                                                  cccGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCg                                                                     <  2:4894513/150‑1 (MQ=255)
                                                                                     ggATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTc                                                                  >  2:1663500/1‑150 (MQ=255)
                                                                                          tctcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCt                                                             <  1:506656/150‑1 (MQ=255)
                                                                                           ctcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTc                                                            >  1:4384347/1‑150 (MQ=255)
                                                                                            tcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGGAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCg                                                           >  1:4103048/1‑150 (MQ=255)
                                                                                            tcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATAAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCAGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCg                                                           >  2:2405247/1‑150 (MQ=255)
                                                                                             cAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGc                                                          >  2:2043661/1‑150 (MQ=255)
                                                                                               aCCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCaa                                                        <  1:5024422/150‑1 (MQ=255)
                                                                                                ccAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGTAGCAGGACTTCATCAAACCGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTATGTTAAGCCAATAACCCCGTTCCGCCTCGCTAg                                                       <  2:434019/150‑1 (MQ=255)
                                                                                                 cAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAgg                                                      >  2:1200258/1‑150 (MQ=255)
                                                                                                  aaaGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCACggg                                                     >  2:2081580/1‑150 (MQ=255)
                                                                                                  aaaGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAggg                                                     >  1:772532/1‑150 (MQ=255)
                                                                                                    agagAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGc                                                   <  1:1520541/150‑1 (MQ=255)
                                                                                                      agaAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAACTCAACGCCGAGAAGCAGGACTTCATCACAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCgg                                                 <  2:2944909/150‑1 (MQ=255)
                                                                                                      agaAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCgg                                                 >  1:1463948/1‑150 (MQ=255)
                                                                                                        aaaGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGaa                                               >  1:2702837/1‑150 (MQ=255)
                                                                                                        aaaGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGaa                                               <  2:401314/150‑1 (MQ=255)
                                                                                                         aaGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGaaa                                              >  2:1009237/1‑150 (MQ=255)
                                                                                                           gCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGACAac                                            >  2:1370552/1‑150 (MQ=255)
                                                                                                           gCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAac                                            <  2:1890309/150‑1 (MQ=255)
                                                                                                            cccAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAaca                                           <  2:1424314/150‑1 (MQ=255)
                                                                                                                gcagGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAAt                                       <  1:2170981/150‑1 (MQ=255)
                                                                                                                 cagGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATg                                      <  1:403274/150‑1 (MQ=255)
                                                                                                                      cATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTg                                 >  1:2613763/1‑150 (MQ=255)
                                                                                                                          ggCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAAAGGTGATCCCTCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAACACACAATGGAGTGAata                             >  2:1571679/1‑150 (MQ=255)
                                                                                                                          ggCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAata                             <  1:1305210/150‑1 (MQ=255)
                                                                                                                          ggCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAata                             >  2:3693355/1‑150 (MQ=255)
                                                                                                                          ggCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAata                             <  1:3831764/150‑1 (MQ=255)
                                                                                                                           gCCTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAatat                            <  2:2143906/150‑1 (MQ=255)
                                                                                                                           gCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAatat                            <  2:905651/150‑1 (MQ=255)
                                                                                                                           gCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAatat                            <  1:3504300/150‑1 (MQ=255)
                                                                                                                           gCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAatat                            <  1:2686231/150‑1 (MQ=255)
                                                                                                                           gCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAatat                            <  1:4110150/150‑1 (MQ=255)
                                                                                                                            ccTGAACATGGAACAAATCAAGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATg                           >  1:978126/1‑150 (MQ=255)
                                                                                                                             cTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGa                          >  2:4448047/1‑150 (MQ=255)
                                                                                                                              tGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCACGCTGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAg                         <  2:3762877/150‑1 (MQ=255)
                                                                                                                                 aCATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGt                      >  1:1064431/1‑150 (MQ=255)
                                                                                                                                  cATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTa                     >  2:1229936/1‑150 (MQ=255)
                                                                                                                                   aTGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTAt                    <  1:4254805/150‑1 (MQ=255)
                                                                                                                                     ggAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTa                  >  1:2957755/1‑150 (MQ=255)
                                                                                                                                     ggAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTa                  >  1:2566/1‑150 (MQ=255)
                                                                                                                                          aaaTCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTAcgttc             <  1:3565866/150‑1 (MQ=255)
                                                                                                                                           aaTCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTAcgttcg            <  2:2792090/150‑1 (MQ=255)
                                                                                                                                               aaGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGttat        >  2:4526884/1‑150 (MQ=255)
                                                                                                                                                aGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGttatt       >  2:3824422/1‑150 (MQ=255)
                                                                                                                                                aGGCCTAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGttatt       >  2:3140840/1‑150 (MQ=255)
                                                                                                                                                  gCCGAGAAGCAGGACTTCATCAACACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGTTATTaa     <  2:1793323/150‑1 (MQ=255)
                                                                                                                                                     gagaAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGTTATTAACTg  <  1:4834817/150‑1 (MQ=255)
                                                                                                                                                     gagaAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGTTATTAACTg  >  1:2926248/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGAATATGAGCGTATTACGTTCGTTATTAACTG  >  CP009273/4278902‑4279200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑