Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,458,254 T→A 92.8% V271V (GTT→GTA)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,458,2540TA92.8% 901.8 / 44.0 265V271V (GTT→GTA) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (6/13);  new base A (133/113);  total (139/126)
Fisher's exact test for biased strand distribution p-value = 9.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 265 total aligned reads displayed.

CTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTT  >  CP009273/4458109‑4458400
                                                                                                                                                 |                                                                                                                                                  
cTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTg                                                                                                                                                >  2:1766919/1‑150 (MQ=255)
 tACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGa                                                                                                                                               >  2:580955/1‑150 (MQ=255)
  aCCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAg                                                                                                                                              >  2:920842/1‑150 (MQ=255)
   ccACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGc                                                                                                                                             >  2:2819591/1‑150 (MQ=255)
    cACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCa                                                                                                                                            >  2:4647219/1‑150 (MQ=255)
     aCTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAg                                                                                                                                           >  1:4671412/1‑150 (MQ=255)
     aCTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAg                                                                                                                                           >  2:1874882/1‑150 (MQ=255)
      cTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGGAAGTGAGCAgg                                                                                                                                          >  2:3456651/1‑150 (MQ=255)
        cGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaa                                                                                                                                        >  2:3086529/1‑150 (MQ=255)
         gCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaaa                                                                                                                                       <  2:4907523/150‑1 (MQ=255)
         gCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGaaa                                                                                                                                       <  2:3582277/150‑1 (MQ=255)
             gCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGa                                                                                                                                   <  1:4416688/150‑1 (MQ=255)
               cGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGc                                                                                                                                 >  2:604498/1‑150 (MQ=255)
               cGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGc                                                                                                                                 <  1:2582546/150‑1 (MQ=255)
                ggAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGcc                                                                                                                                >  1:4759351/1‑150 (MQ=255)
                 gAGCACAGCAATCCGCGGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCCAGGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCg                                                                                                                               >  2:2298777/1‑150 (MQ=255)
                        gCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCt                                                                                                                        <  1:4818803/150‑1 (MQ=255)
                        gCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCt                                                                                                                        <  1:1614972/150‑1 (MQ=255)
                              cGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGc                                                                                                                  >  1:1028663/1‑150 (MQ=255)
                              cGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGc                                                                                                                  <  2:4029480/150‑1 (MQ=255)
                                 tGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAg                                                                                                               >  2:2595674/1‑150 (MQ=255)
                                     gTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGAt                                                                                                           >  2:1092481/1‑150 (MQ=255)
                                      tGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATc                                                                                                          <  1:2278835/150‑1 (MQ=255)
                                      tGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATc                                                                                                          <  1:2278877/150‑1 (MQ=255)
                                         gACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGc                                                                                                       <  2:2008730/150‑1 (MQ=255)
                                         gACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGc                                                                                                       <  1:3859581/150‑1 (MQ=255)
                                           cacGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCcg                                                                                                     >  2:2987916/1‑150 (MQ=255)
                                             cGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCcgcg                                                                                                   >  1:4934433/1‑150 (MQ=255)
                                             cGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCcgcg                                                                                                   >  1:1112187/1‑150 (MQ=255)
                                               cTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTc                                                                                                 >  1:4009067/1‑150 (MQ=255)
                                                tgtgTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCa                                                                                                >  1:2949566/1‑150 (MQ=255)
                                                  tgtTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGt                                                                                              <  1:397661/150‑1 (MQ=255)
                                                    ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                            >  1:624744/1‑150 (MQ=255)
                                                    ttttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAt                                                                                            <  2:1878322/150‑1 (MQ=255)
                                                     tttATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtg                                                                                           >  1:1315728/1‑150 (MQ=255)
                                                        aTGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgctg                                                                                        <  2:3243138/150‑1 (MQ=255)
                                                        aTGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTAtgctg                                                                                        <  1:306539/150‑1 (MQ=255)
                                                          gggCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATgctgct                                                                                      <  1:150280/150‑1 (MQ=255)
                                                           ggCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGctgctg                                                                                     <  1:4106153/150‑1 (MQ=255)
                                                           ggCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGctgctg                                                                                     <  2:4315747/150‑1 (MQ=255)
                                                                  ccGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCt                                                                              <  2:1222666/150‑1 (MQ=255)
                                                                   cGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCtt                                                                             >  2:506637/1‑150 (MQ=255)
                                                                      ggtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTAt                                                                          <  1:1850814/150‑1 (MQ=255)
                                                                       gtgAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATg                                                                         >  2:4042126/1‑150 (MQ=255)
                                                                          aGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTg                                                                      <  1:2371284/150‑1 (MQ=255)
                                                                           gCGGCACGGCACAAGCAATGGTGAGTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTgg                                                                     <  1:1748987/150‑1 (MQ=255)
                                                                            cggcacggcaCAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGt                                                                    >  1:4442223/1‑150 (MQ=255)
                                                                            cggcacggcaCAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGt                                                                    >  2:3159150/1‑150 (MQ=255)
                                                                             ggcacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTg                                                                   <  1:1945536/150‑1 (MQ=255)
                                                                               cacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGAt                                                                 >  1:4854466/1‑150 (MQ=255)
                                                                               cacggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGAt                                                                 >  2:4725731/1‑150 (MQ=255)
                                                                                acggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATg                                                                >  2:3045358/1‑150 (MQ=255)
                                                                                  ggcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGc                                                              <  2:1187926/150‑1 (MQ=255)
                                                                                   gcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCa                                                             <  2:1354755/150‑1 (MQ=255)
                                                                                   gcacAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCa                                                             >  1:3192382/1‑150 (MQ=255)
                                                                                     acaAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCAcc                                                           >  2:294738/1‑150 (MQ=255)
                                                                                     acaAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGGAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTGATGCTGGTGATGGCAcc                                                           >  1:924847/1‑150 (MQ=255)
                                                                                         gCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtg                                                       <  2:2181863/150‑1 (MQ=255)
                                                                                         gCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtg                                                       <  2:1382329/150‑1 (MQ=255)
                                                                                         gCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtg                                                       >  1:2642354/1‑150 (MQ=255)
                                                                                           aaTGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCggtggt                                                     >  2:1147827/1‑150 (MQ=255)
                                                                                            aTGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGgtggtg                                                    >  1:3795636/1‑150 (MQ=255)
                                                                                             tGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGc                                                   >  2:454637/1‑150 (MQ=255)
                                                                                                tGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGt                                                >  1:1928476/1‑150 (MQ=255)
                                                                                                tGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGt                                                <  1:3712903/150‑1 (MQ=255)
                                                                                                   ttGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTaa                                             >  1:1486338/1‑150 (MQ=255)
                                                                                                     gCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATc                                           >  1:4126847/1‑150 (MQ=255)
                                                                                                      cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCa                                          >  1:672879/1‑150 (MQ=255)
                                                                                                      cTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCa                                          >  2:1220283/1‑150 (MQ=255)
                                                                                                         cAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTGTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATg                                       >  2:2213014/1‑150 (MQ=255)
                                                                                                          aGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATgg                                      >  2:3139900/1‑150 (MQ=255)
                                                                                                           ggTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGt                                     >  2:2431824/1‑150 (MQ=255)
                                                                                                           ggTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGt                                     <  2:3897011/150‑1 (MQ=255)
                                                                                                            gTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt                                    <  1:2084602/150‑1 (MQ=255)
                                                                                                            gTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGtt                                    <  1:2084426/150‑1 (MQ=255)
                                                                                                                cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTacac                                <  1:528837/150‑1 (MQ=255)
                                                                                                                cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTacac                                >  2:1270978/1‑150 (MQ=255)
                                                                                                                cAATACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTacac                                <  1:3136414/150‑1 (MQ=255)
                                                                                                                 aaTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACAcc                               >  2:1629409/1‑150 (MQ=255)
                                                                                                                  aTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCACTGGTTACACCa                              >  2:1043541/1‑150 (MQ=255)
                                                                                                                  aTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                              >  2:3350501/1‑150 (MQ=255)
                                                                                                                  aTACCTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCa                              <  2:2195677/150‑1 (MQ=255)
                                                                                                                     ccTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAg                           <  1:59067/150‑1 (MQ=255)
                                                                                                                      cTGGTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAgg                          <  2:1418138/150‑1 (MQ=255)
                                                                                                                        ggTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACAcc                               <  1:1303865/143‑1 (MQ=255)
                                                                                                                        ggTTTGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCg                        >  1:1389634/1‑150 (MQ=255)
                                                                                                                          tttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc                      <  1:2987916/150‑1 (MQ=255)
                                                                                                                          tttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc                      <  1:2819591/150‑1 (MQ=255)
                                                                                                                          gtgggTCCACTGGCGGGGCGTGTAAGTGAGCAGGAACGCGAGCCGACTGCCTGTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGCTTCGCTTTAGGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGAc                      <  2:343781/147‑1 (MQ=255)
                                                                                                                           ttGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACt                     >  2:4152451/1‑150 (MQ=255)
                                                                                                                            tGGTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtg                    >  2:4961873/1‑150 (MQ=255)
                                                                                                                              gTCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtggt                  >  1:3456020/1‑150 (MQ=255)
                                                                                                                               tCAACTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACtggtg                 <  1:3596738/150‑1 (MQ=255)
                                                                                                                                 aaCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTggg               >  2:779492/1‑150 (MQ=255)
                                                                                                                                  aCTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGa              <  1:3029157/150‑1 (MQ=255)
                                                                                                                                   cTGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTGTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGaa             >  2:3804097/1‑150 (MQ=255)
                                                                                                                                    tGGCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAg            <  1:4977111/150‑1 (MQ=255)
                                                                                                                                      gCGGGGCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcg          <  2:2609289/150‑1 (MQ=255)
                                                                                                                                        ggggCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAgcggc        >  2:4537983/1‑150 (MQ=255)
                                                                                                                                           gCGTGTAAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCt     <  1:4638436/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATTGCTACAGGTGCCAATACCTGGTTTGGTCAACTGGCGGGGCGTGTTAGTGAGCAGGAAAGCGAGCCGAATGCCTTTCAGCAAGGGATCAGCCGCGTCAGTATGCTGCTGATTCGCTTTATGCTGGTGATGGCACCGGTGGTGCTGTTAATCAATGGTTACACCAAAGGCGACTGGTGGGAAGCGGCGCTGTT  >  CP009273/4458109‑4458400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑