Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 334,250 G→T 10.6% R72L (CGT→CTT)  yahG → DUF1116 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273334,2500GT10.6% 817.8 / 56.6 242R72L (CGT→CTT) yahGDUF1116 family protein
Reads supporting (aligned to +/- strand):  ref base G (119/97);  new base T (13/13);  total (132/110)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

GACCTGAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAGCGC  >  CP009273/334102‑334399
                                                                                                                                                    |                                                                                                                                                     
gACCTGAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGt                                                                                                                                                      >  2:2485125/1‑150 (MQ=255)
 aCCTGAAACAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGta                                                                                                                                                     <  2:3532619/150‑1 (MQ=255)
  ccTGAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGtat                                                                                                                                                    >  2:4960632/1‑150 (MQ=255)
    tGAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGtatta                                                                                                                                                  <  2:4813048/150‑1 (MQ=255)
    tGAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGtatta                                                                                                                                                  >  1:3522684/1‑150 (MQ=255)
     gAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGtattat                                                                                                                                                 <  1:1676589/150‑1 (MQ=255)
         aaGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAg                                                                                                                                             <  1:3544486/150‑1 (MQ=255)
          agcagcATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGt                                                                                                                                            >  1:3834390/1‑150 (MQ=255)
          agcagcATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGt                                                                                                                                            >  2:298320/1‑150 (MQ=255)
            cagcaTGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCg                                                                                                                                          <  1:774183/150‑1 (MQ=255)
              gcaTGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCa                                                                                                                                        <  2:1064893/150‑1 (MQ=255)
              gcaTGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCa                                                                                                                                        <  1:54559/150‑1 (MQ=255)
               caTGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCAt                                                                                                                                       >  1:5029549/1‑150 (MQ=255)
                aTGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATc                                                                                                                                      >  2:3666956/1‑150 (MQ=255)
                 tGTAGAAGTGACTCAACACGACAGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGAGCAGGCGCTGGATAACATTGACGATTCGCCGCTGGCGGACAAACTCGCCGCCGCTAAACAGCAGGCGCTGGAGCGTATTATCCAGTCGCATcc                                                                                                                                     >  1:3322315/1‑150 (MQ=255)
                   tAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCgg                                                                                                                                   >  2:2715065/1‑150 (MQ=255)
                   tAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCgg                                                                                                                                   >  2:1663922/1‑150 (MQ=255)
                        gTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTg                                                                                                                              >  1:1150859/1‑150 (MQ=255)
                          gACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGAt                                                                                                                            <  1:4286154/150‑1 (MQ=255)
                            cTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTg                                                                                                                          >  2:754985/1‑150 (MQ=255)
                               aaCTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGtt                                                                                                                       >  2:2603690/1‑150 (MQ=255)
                                  tCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTg                                                                                                                    <  2:4448353/150‑1 (MQ=255)
                                  tCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTg                                                                                                                    >  1:2753028/1‑150 (MQ=255)
                                  tCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTg                                                                                                                    >  1:2752991/1‑150 (MQ=255)
                                   cGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGa                                                                                                                   >  1:4831515/1‑150 (MQ=255)
                                   cGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGa                                                                                                                   >  1:2114233/1‑150 (MQ=255)
                                    gACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGAt                                                                                                                  <  2:209105/150‑1 (MQ=255)
                                     aCTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATc                                                                                                                 <  2:1624494/150‑1 (MQ=255)
                                       tGGACGGCGACGGGTCAGTGCAATATTCTGGTGGTGCAGGCGATGTATAACATTGCCGATTCGCCGCTTGCGGACAAAATCGCCGCCGCTTACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAg                                                                                                               <  2:5824/150‑1 (MQ=255)
                                        ggACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAgg                                                                                                              >  1:3107086/1‑150 (MQ=255)
                                           cgccgcCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCTGGAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGa                                                                                                           <  2:3600803/150‑1 (MQ=255)
                                             ccgccgGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGAtt                                                                                                         >  1:2379273/1‑150 (MQ=255)
                                             ccgccgAGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTACCCAGCAGGCGCTGGTGCGGAGTATCCAGTCGCATCCGGTGCTGCGTGGGTTTGAGCCGGCGagt                                                                                                         >  1:5084730/1‑148 (MQ=255)
                                              cgccgGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTa                                                                                                        <  2:3410982/150‑1 (MQ=255)
                                               gccgGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTaa                                                                                                       <  1:1343592/150‑1 (MQ=255)
                                               gccgGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTaa                                                                                                       >  2:4128788/1‑150 (MQ=255)
                                                ccgGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAAc                                                                                                      <  2:3361077/150‑1 (MQ=255)
                                                  ggggCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACgt                                                                                                    >  2:3933989/1‑150 (MQ=255)
                                                     gcagggcaATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACgtggt                                                                                                 <  2:2200579/150‑1 (MQ=255)
                                                         ggcaATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCg                                                                                             >  1:2692778/1‑150 (MQ=255)
                                                         ggcaATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCg                                                                                             <  2:2813929/150‑1 (MQ=255)
                                                          gcaATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCgg                                                                                            <  1:361211/150‑1 (MQ=255)
                                                             atatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCa                                                                                         >  2:161836/1‑150 (MQ=255)
                                                             atatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCa                                                                                         >  2:1874176/1‑150 (MQ=255)
                                                             atatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCa                                                                                         >  2:200702/1‑150 (MQ=255)
                                                             atatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCa                                                                                         >  1:4990369/1‑150 (MQ=255)
                                                             atatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCa                                                                                         <  1:4180610/150‑1 (MQ=255)
                                                             atatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCa                                                                                         <  2:4538037/150‑1 (MQ=255)
                                                              tatGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCAt                                                                                        >  1:3418691/1‑150 (MQ=255)
                                                                 gCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGAc                                                                                     >  1:2164631/1‑150 (MQ=255)
                                                                   aGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACgg                                                                                   >  2:2614096/1‑150 (MQ=255)
                                                                      tggtgCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGa                                                                                <  1:1179851/150‑1 (MQ=255)
                                                                         tgCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGaaaa                                                                             >  2:5062986/1‑150 (MQ=255)
                                                                          gCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAAc                                                                            >  1:2783230/1‑150 (MQ=255)
                                                                          gCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAAc                                                                            <  2:515551/150‑1 (MQ=255)
                                                                            aGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCa                                                                          >  1:928481/1‑150 (MQ=255)
                                                                            aGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCa                                                                          <  1:1401043/150‑1 (MQ=255)
                                                                              gcgcTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAtt                                                                        <  1:2715065/150‑1 (MQ=255)
                                                                               cgcTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAttc                                                                       <  1:4699896/150‑1 (MQ=255)
                                                                                gcTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAttct                                                                      <  1:843146/150‑1 (MQ=255)
                                                                                gcTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAttct                                                                      >  1:3922735/1‑150 (MQ=255)
                                                                                 cTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAttctt                                                                     >  2:115456/1‑150 (MQ=255)
                                                                                  tGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAttcttc                                                                    >  2:4615228/1‑150 (MQ=255)
                                                                                  tGGATAACATTGCCGATTCGCCGCTGGCGGACAAAAACGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCAttcttc                                                                    >  1:80339/1‑150 (MQ=255)
                                                                                   ggATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCa                                                                   <  1:5017621/150‑1 (MQ=255)
                                                                                    gATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCAc                                                                  <  2:3774355/150‑1 (MQ=255)
                                                                                     aTAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACg                                                                 >  1:3975457/1‑150 (MQ=255)
                                                                                       aaCATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGcc                                                               <  2:3503549/150‑1 (MQ=255)
                                                                                       aaCATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGcc                                                               >  1:1749127/1‑150 (MQ=255)
                                                                                        aCATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCg                                                              <  1:2996765/150‑1 (MQ=255)
                                                                                             gCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGgccg                                                         >  2:2693500/1‑150 (MQ=255)
                                                                                                 aTTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGa                                                     <  2:561513/150‑1 (MQ=255)
                                                                                                  ttCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGAt                                                    >  2:119158/1‑150 (MQ=255)
                                                                                                   tCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATc                                                   <  2:3522684/150‑1 (MQ=255)
                                                                                                    cgccgcTGGCGGACAAAATCGCCGCCGCTAACCCGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCa                                                  <  2:241957/150‑1 (MQ=255)
                                                                                                    cgccgcTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCa                                                  >  1:2926368/1‑150 (MQ=255)
                                                                                                       cgcTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCt                                               <  2:1350509/150‑1 (MQ=255)
                                                                                                        gcTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTCTTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTg                                              >  2:3155561/1‑150 (MQ=255)
                                                                                                        gcTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTg                                              >  2:3155694/1‑150 (MQ=255)
                                                                                                        gcTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTg                                              <  1:4557544/150‑1 (MQ=255)
                                                                                                          tGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTggg                                            <  1:1816023/150‑1 (MQ=255)
                                                                                                            gCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGaa                                          <  1:3208768/150‑1 (MQ=255)
                                                                                                              ggACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGaaaa                                        >  1:709454/1‑150 (MQ=255)
                                                                                                                 cAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAAt                                     >  2:2316758/1‑150 (MQ=255)
                                                                                                                  aaaaTCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAAtg                                    <  2:4871127/150‑1 (MQ=255)
                                                                                                                    aaTCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAAtgtg                                  >  1:2679014/1‑150 (MQ=255)
                                                                                                                            ccgcTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGa                          <  1:1517640/150‑1 (MQ=255)
                                                                                                                               cTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGa                       >  2:3507029/1‑150 (MQ=255)
                                                                                                                                   ccAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAgg                   <  1:2603690/150‑1 (MQ=255)
                                                                                                                                   ccAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAgg                   <  1:3407053/150‑1 (MQ=255)
                                                                                                                                    cagcagGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGgc                  <  1:298320/150‑1 (MQ=255)
                                                                                                                                    cagcagGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGgc                  <  2:4078928/150‑1 (MQ=255)
                                                                                                                                    cagcagGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGgc                  <  1:2012969/150‑1 (MQ=255)
                                                                                                                                       cagGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGcgcg               <  1:4338916/150‑1 (MQ=255)
                                                                                                                                        agGCGCTGGAGCTTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCgg              >  1:4410041/1‑150 (MQ=255)
                                                                                                                                        agGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCgg              >  2:2064308/1‑150 (MQ=255)
                                                                                                                                         ggCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGt             >  2:2975960/1‑150 (MQ=255)
                                                                                                                                                 aGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAg     <  2:4242502/150‑1 (MQ=255)
                                                                                                                                                    gTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAgcgc  >  1:3655003/1‑150 (MQ=255)
                                                                                                                                                    gTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAgcgc  >  1:1056634/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GACCTGAAAAAGCAGCATGTAGAAGTGACTCAACTCGACTGGACGCCGCCGGGGCAGGGCAATATGCAGGTGGTGCAGGCGCTGGATAACATTGCCGATTCGCCGCTGGCGGACAAAATCGCCGCCGCTAACCAGCAGGCGCTGGAGCGTATTATCCAGTCGCATCCGGTGCTGATTGGTTTTGATCAGGCGATTAACGTGGTGCCGGGCATGACGGCGAAAACCATTCTTCACGCCGGGCCGCCGATCACCTGGGAAAAAATGTGCGGCGCGATGAAAGGCGCGGTCACCGGAGCGC  >  CP009273/334102‑334399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑