BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,471,490	G→T	5.3%	P722Q (CCG→CAG)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,471,490	0	G	T	5.3%	802.0 / 19.9	226	P722Q (CCG→CAG)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (108/106); new base T (5/7); total (113/113)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

TGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCC  >  CP009273/4471341‑4471639

tGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCg                                                                                                                                                       >  1:59337/1‑150 (MQ=255)

tGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCg                                                                                                                                                       >  2:2935846/1‑150 (MQ=255)

    aCCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCtt                                                                                                                                                   >  1:2850182/1‑150 (MQ=255)

      cGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTgg                                                                                                                                                 >  1:1186588/1‑150 (MQ=255)

      cGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTgg                                                                                                                                                 >  1:1669333/1‑150 (MQ=255)

        cAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTc                                                                                                                                               >  2:2885927/1‑150 (MQ=255)

         aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCTGCTTGGTCa                                                                                                                                              <  2:2849966/150‑1 (MQ=255)

         aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCTGCTTGGTCa                                                                                                                                              <  2:4926324/150‑1 (MQ=255)

         aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCa                                                                                                                                              >  2:2418843/1‑150 (MQ=255)

         aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGATCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCa                                                                                                                                              >  1:3968745/1‑150 (MQ=255)

         aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAAAGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGATCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCa                                                                                                                                              >  1:3786841/1‑150 (MQ=255)

           aGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGc                                                                                                                                            >  2:3315070/1‑150 (MQ=255)

           aGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGc                                                                                                                                            >  2:4873269/1‑150 (MQ=255)

            gTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCt                                                                                                                                           <  1:3838380/150‑1 (MQ=255)

            gTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCt                                                                                                                                           <  1:3838002/150‑1 (MQ=255)

               cTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCga                                                                                                                                        >  2:3620229/1‑150 (MQ=255)

                tttCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCgag                                                                                                                                       <  2:2082796/150‑1 (MQ=255)

                tttCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCgag                                                                                                                                       <  2:4669369/150‑1 (MQ=255)

                  tCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGAt                                                                                                                                     <  2:3264422/150‑1 (MQ=255)

                    acacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATAc                                                                                                                                   <  1:3552612/150‑1 (MQ=255)

                     cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATAcc                                                                                                                                  >  2:407045/1‑150 (MQ=255)

                       cGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAg                                                                                                                                >  2:2522040/1‑150 (MQ=255)

                       cGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAg                                                                                                                                <  1:1494266/150‑1 (MQ=255)

                         cTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTc                                                                                                                              >  2:3956352/1‑150 (MQ=255)

                                 tGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACt                                                                                                                      >  1:1979580/1‑150 (MQ=255)

                                   gTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTgg                                                                                                                    <  1:2611657/150‑1 (MQ=255)

                                   gTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTgg                                                                                                                    <  1:3961238/150‑1 (MQ=255)

                                         gTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAg                                                                                                              <  1:2541772/150‑1 (MQ=255)

                                         gTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAg                                                                                                              >  2:1676036/1‑150 (MQ=255)

                                               aaCGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAAc                                                                                                        <  1:1261442/150‑1 (MQ=255)

                                                aCGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACt                                                                                                       <  1:259832/150‑1 (MQ=255)

                                                 cGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTc                                                                                                      <  1:3945865/150‑1 (MQ=255)

                                                   gAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCAt                                                                                                    >  1:3594920/1‑150 (MQ=255)

                                                    aaTGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATa                                                                                                   <  2:1962473/150‑1 (MQ=255)

                                                      tGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACa                                                                                                 <  1:127756/150‑1 (MQ=255)

                                                      tGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACa                                                                                                 <  1:2743876/150‑1 (MQ=255)

                                                       gATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAg                                                                                                <  1:2318945/150‑1 (MQ=255)

                                                       gATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAg                                                                                                >  2:5018323/1‑150 (MQ=255)

                                                       gATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAg                                                                                                <  1:1109091/150‑1 (MQ=255)

                                                          cGGATGCGCGAGGCGCAGCAGACCTTCCAGTTCAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCAGAGAACTGGTTCCAGGTGTACTCATACAGAAt                                                                                             <  1:826525/150‑1 (MQ=255)

                                                                   gAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCGTACAGAATGCCTgcggc                                                                                    >  1:1912550/1‑150 (MQ=255)

                                                                    aGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTgcggcg                                                                                   <  2:1226489/150‑1 (MQ=255)

                                                                     ggCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGa                                                                                  <  2:2618980/150‑1 (MQ=255)

                                                                       cgcAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGata                                                                                <  2:2562434/150‑1 (MQ=255)

                                                                       cgcAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGaga                                                                                >  2:3333380/1‑148 (MQ=255)

                                                                        gcagcaGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGatat                                                                               <  1:2146450/150‑1 (MQ=255)

                                                                         cagcagACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATc                                                                              >  2:2305685/1‑150 (MQ=255)

                                                                         cagcagACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATc                                                                              <  1:1562776/150‑1 (MQ=255)

                                                                         cagcagACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCGTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATc                                                                              <  1:3632795/150‑1 (MQ=255)

                                                                          agcagACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCg                                                                             <  1:1244680/150‑1 (MQ=255)

                                                                          agcagACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCg                                                                             >  1:1082107/1‑150 (MQ=255)

                                                                              gACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGc                                                                         <  2:2129924/150‑1 (MQ=255)

                                                                                ccTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCgg                                                                       <  2:3854625/150‑1 (MQ=255)

                                                                                  ttCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGaa                                                                     <  1:980633/150‑1 (MQ=255)

                                                                                     cAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCt                                                                  <  2:1550619/150‑1 (MQ=255)

                                                                                        tACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCCGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTc                                                               >  2:4203882/1‑150 (MQ=255)

                                                                                         aCAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTcc                                                              <  2:319475/150‑1 (MQ=255)

                                                                                         aCAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTcc                                                              >  2:4189105/1‑150 (MQ=255)

                                                                                         aCAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTcc                                                              <  1:4840493/150‑1 (MQ=255)

                                                                                          cAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCa                                                             >  2:3374132/1‑150 (MQ=255)

                                                                                          cAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCa                                                             <  2:3068949/150‑1 (MQ=255)

                                                                                             tCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAgcg                                                          <  1:5015970/150‑1 (MQ=255)

                                                                                              cACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAgcgc                                                         >  1:4735706/1‑150 (MQ=255)

                                                                                                ccAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCtt                                                       <  2:3722390/150‑1 (MQ=255)

                                                                                                ccAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCtt                                                       <  1:23370/150‑1 (MQ=255)

                                                                                                ccAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCtt                                                       <  2:4606473/150‑1 (MQ=255)

                                                                                                ccAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCtt                                                       >  2:2793238/1‑150 (MQ=255)

                                                                                                ccAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCtt                                                       <  2:1124183/150‑1 (MQ=255)

                                                                                                 cAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTc                                                      <  1:4164373/150‑1 (MQ=255)

                                                                                                 cAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTc                                                      >  2:1537167/1‑150 (MQ=255)

                                                                                                 cAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTc                                                      <  1:1177603/150‑1 (MQ=255)

                                                                                                 cAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTc                                                      <  1:309139/150‑1 (MQ=255)

                                                                                                 cAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTc                                                      <  1:1177558/150‑1 (MQ=255)

                                                                                                  aGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCAGTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTcg                                                     >  2:4359258/1‑150 (MQ=255)

                                                                                                      tATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGt                                                 >  2:2787738/1‑150 (MQ=255)

                                                                                                      tATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGt                                                 <  1:1477161/150‑1 (MQ=255)

                                                                                                       aTGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTa                                                <  1:5190284/150‑1 (MQ=255)

                                                                                                         ggCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACg                                              <  1:2885927/150‑1 (MQ=255)

                                                                                                         ggCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACg                                              >  2:916993/1‑150 (MQ=255)

                                                                                                             aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGACGCTGTCCAGCGCTTCGCGGTACGCgtt                                          >  2:4720203/1‑150 (MQ=255)

                                                                                                             aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCttt                                          >  2:819389/1‑150 (MQ=255)

                                                                                                             aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCttt                                          <  1:2352147/150‑1 (MQ=255)

                                                                                                             aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCttt                                          <  2:2976196/150‑1 (MQ=255)

                                                                                                             aGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCttt                                          >  2:2898970/1‑150 (MQ=255)

                                                                                                               tACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCTGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGa                                        >  1:1035717/1‑150 (MQ=255)

                                                                                                                 ccGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATg                                      <  1:1131998/150‑1 (MQ=255)

                                                                                                                      cAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTg                                 >  2:708553/1‑150 (MQ=255)

                                                                                                                      cAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTg                                 <  2:4787693/150‑1 (MQ=255)

                                                                                                                       aGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTgg                                >  1:3252023/1‑150 (MQ=255)

                                                                                                                        gTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGt                               >  2:366643/1‑150 (MQ=255)

                                                                                                                        gTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGt                               >  2:3633231/1‑150 (MQ=255)

                                                                                                                        gTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGt                               <  2:2850182/150‑1 (MQ=255)

                                                                                                                            tGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGaa                           <  2:1628532/150‑1 (MQ=255)

                                                                                                                              cTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACt                         <  1:3000907/150‑1 (MQ=255)

                                                                                                                                  ggTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGc                     >  2:5010001/1‑150 (MQ=255)

                                                                                                                                    tGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCa                   >  1:3124897/1‑150 (MQ=255)

                                                                                                                                        aCCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAAt               >  2:2313190/1‑150 (MQ=255)

                                                                                                                                                 tACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTc      <  2:2168575/150‑1 (MQ=255)

                                                                                                                                                   ccGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCg    >  2:4654750/1‑150 (MQ=255)

                                                                                                                                                    cGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGc   <  2:2912566/150‑1 (MQ=255)

TGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTCTGCTTCGGTGCCACCGTTCATTACCGGCTTGGTCAGCTCGAGATACCAGTCACAGAACTGGTTCCAGGTGAACTCATACAGAATGCCTGCGGCGATATCGAAGCGGAAGCTGTCCAGCGCTTCGCGGTACGCTTTGATGGTCTGGTTGAACTCCGCCAGAATCCAGCGGTCCGCC  >  CP009273/4471341‑4471639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`