Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,516,734 G→T 26.7% F207L (TTC→TTA)  sgcE ← putative epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,516,7340GT26.7% 575.9 / 207.2 273F207L (TTC→TTA) sgcEputative epimerase
Reads supporting (aligned to +/- strand):  ref base G (111/89);  new base T (36/37);  total (147/126)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

ACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCG  >  CP009273/4516586‑4516883
                                                                                                                                                    |                                                                                                                                                     
acgacgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGa                                                                                                                                                      <  1:2454517/150‑1 (MQ=255)
  gacgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAAt                                                                                                                                                    <  2:661116/150‑1 (MQ=255)
  gacgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAAt                                                                                                                                                    >  2:294231/1‑150 (MQ=255)
  gacgGACAGTCGCGTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAAt                                                                                                                                                    <  1:226762/150‑1 (MQ=255)
    cgGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTg                                                                                                                                                  <  2:746440/150‑1 (MQ=255)
     ggACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGa                                                                                                                                                 >  1:3381725/1‑150 (MQ=255)
            cGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAgg                                                                                                                                          >  2:3629196/1‑150 (MQ=255)
            cGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAgg                                                                                                                                          >  1:4027533/1‑150 (MQ=255)
               ttCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTcg                                                                                                                                       >  2:1625553/1‑150 (MQ=255)
               ttCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTcg                                                                                                                                       >  2:1625204/1‑150 (MQ=255)
                    cATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGa                                                                                                                                  >  2:4038139/1‑150 (MQ=255)
                       aGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAg                                                                                                                               <  2:4855714/150‑1 (MQ=255)
                       aGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAg                                                                                                                               >  2:4144535/1‑150 (MQ=255)
                       aGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAg                                                                                                                               >  2:2679243/1‑150 (MQ=255)
                         ccAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTc                                                                                                                             <  2:2656854/150‑1 (MQ=255)
                          cAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTCAGGTCGCGCGATAGTCa                                                                                                                            >  1:2338046/1‑150 (MQ=255)
                          cAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGACTCTGTTGGCTCATCAGACACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCa                                                                                                                            >  1:1531622/1‑150 (MQ=255)
                           aaaaaGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAg                                                                                                                           >  2:4441549/1‑150 (MQ=255)
                           aaaaaGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAg                                                                                                                           >  2:3099853/1‑150 (MQ=255)
                            aaaaGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGa                                                                                                                          >  2:2974210/1‑150 (MQ=255)
                            aaaaGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGa                                                                                                                          >  2:4603898/1‑150 (MQ=255)
                             aaaGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGAt                                                                                                                         <  2:2404480/150‑1 (MQ=255)
                              aaGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATg                                                                                                                        >  1:4620867/1‑150 (MQ=255)
                                gCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGaa                                                                                                                      >  1:2594241/1‑150 (MQ=255)
                                    cATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAgaga                                                                                                                  >  2:1696794/1‑150 (MQ=255)
                                     aTGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGaa                                                                                                                 >  1:3921992/1‑150 (MQ=255)
                                      tGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAAc                                                                                                                >  1:3834653/1‑150 (MQ=255)
                                      tGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAAc                                                                                                                <  2:584944/150‑1 (MQ=255)
                                       ggCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACa                                                                                                               <  1:4168618/150‑1 (MQ=255)
                                          ctgctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAggg                                                                                                            >  2:2259523/1‑150 (MQ=255)
                                            gctgTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGgcg                                                                                                          <  1:2436245/150‑1 (MQ=255)
                                                  tgGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCa                                                                                                    <  1:2896671/150‑1 (MQ=255)
                                                   ggAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTTCAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCaa                                                                                                   <  2:430640/150‑1 (MQ=255)
                                                     agagGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATc                                                                                                 >  2:4802095/1‑150 (MQ=255)
                                                      gagGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCa                                                                                                <  2:5136612/150‑1 (MQ=255)
                                                      gagGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCa                                                                                                <  2:1149578/150‑1 (MQ=255)
                                                           gACGATGTTGCCACAGGTCGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCatc                                                                                           >  2:2314743/1‑149 (MQ=255)
                                                             cGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATg                                                                                         <  1:362042/150‑1 (MQ=255)
                                                              gATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGt                                                                                        >  1:3774061/1‑150 (MQ=255)
                                                              gATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGt                                                                                        >  1:4358500/1‑150 (MQ=255)
                                                              gATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGt                                                                                        >  1:4811400/1‑150 (MQ=255)
                                                                tGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTg                                                                                      >  1:1359881/1‑150 (MQ=255)
                                                                 gTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTgc                                                                                     <  1:2966839/150‑1 (MQ=255)
                                                                   tGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTgcgc                                                                                   >  1:692214/1‑150 (MQ=255)
                                                                    gCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGcc                                                                                  >  2:3551135/1‑150 (MQ=255)
                                                                      cacaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGcccc                                                                                >  2:3223563/1‑150 (MQ=255)
                                                                       acaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCa                                                                               <  1:5085412/150‑1 (MQ=255)
                                                                       acaGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCa                                                                               <  2:329591/150‑1 (MQ=255)
                                                                           gTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGTACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgctg                                                                           <  2:1074974/150‑1 (MQ=255)
                                                                             aGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgctgct                                                                         >  2:1973203/1‑150 (MQ=255)
                                                                             aGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgctgct                                                                         <  1:942080/150‑1 (MQ=255)
                                                                             aGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgctgct                                                                         <  2:3065263/150‑1 (MQ=255)
                                                                             aGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAgctgct                                                                         <  2:4106675/150‑1 (MQ=255)
                                                                              gTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGctgctg                                                                        <  2:4554010/150‑1 (MQ=255)
                                                                                  agcaTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAg                                                                    >  2:3650941/1‑150 (MQ=255)
                                                                                  agcaTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAg                                                                    >  1:112026/1‑150 (MQ=255)
                                                                                  agcaTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAg                                                                    >  2:2155156/1‑150 (MQ=255)
                                                                                    caTCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGtt                                                                  >  2:885643/1‑150 (MQ=255)
                                                                                       cTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCt                                                               >  2:2607310/1‑150 (MQ=255)
                                                                                        tGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCtg                                                              >  1:415158/1‑150 (MQ=255)
                                                                                            tGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGGTGCGCCCCAGCTGCTGCCAGTTGCTGTGCa                                                          <  2:4904606/150‑1 (MQ=255)
                                                                                                  ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGcc                                                    <  1:1960432/150‑1 (MQ=255)
                                                                                                  ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGcc                                                    <  1:79623/150‑1 (MQ=255)
                                                                                                  ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCCGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGcc                                                    >  2:2538127/1‑150 (MQ=255)
                                                                                                  ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGcc                                                    >  1:3363985/1‑150 (MQ=255)
                                                                                                   gTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCa                                                   <  2:230260/150‑1 (MQ=255)
                                                                                                   gTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCa                                                   <  2:5019395/150‑1 (MQ=255)
                                                                                                   gTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCa                                                   >  2:3380624/1‑150 (MQ=255)
                                                                                                     cAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGc                                                 >  2:1349401/1‑150 (MQ=255)
                                                                                                     cAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGc                                                 >  2:3945580/1‑150 (MQ=255)
                                                                                                       ggTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGt                                               <  1:3729860/150‑1 (MQ=255)
                                                                                                          ctctGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAAt                                            >  1:4945098/1‑150 (MQ=255)
                                                                                                           tctGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAAtt                                           <  1:3294148/150‑1 (MQ=255)
                                                                                                             tGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTcc                                         >  1:2285303/1‑150 (MQ=255)
                                                                                                               ttGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGGCGCGCGATAGGCAGAGGAAGAGAACAGGGCGCGCCCAATCCCCCTAGGTTGCGCCCCTGCTGCTGCCCGTTGCTGTGCAGCTGGCCGCGTACTTCCAc                                       >  2:3755549/1‑150 (MQ=255)
                                                                                                                    tcatcaGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCg                                  <  2:5183159/150‑1 (MQ=255)
                                                                                                                    tcatcaGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCg                                  <  1:3107807/150‑1 (MQ=255)
                                                                                                                      atcaGAAACAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGc                                >  1:3481148/1‑150 (MQ=255)
                                                                                                                        caGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGccc                              >  2:1923902/1‑150 (MQ=255)
                                                                                                                        caGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGccc                              <  1:2282003/150‑1 (MQ=255)
                                                                                                                           aaaCAATCTCCTCTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGc                           >  1:442939/1‑150 (MQ=255)
                                                                                                                            aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCa                          <  1:2061626/150‑1 (MQ=255)
                                                                                                                              cAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACt                        >  1:5110248/1‑150 (MQ=255)
                                                                                                                               aaTCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTc                       <  1:4261018/150‑1 (MQ=255)
                                                                                                                                  ctcctcTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGt                    >  2:5094159/1‑150 (MQ=255)
                                                                                                                                    cctcTTACAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCt                  >  2:1488193/1‑150 (MQ=255)
                                                                                                                                     ctctTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACGGGGCGCGCCCAATCACCATATGTGGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                 <  2:5120565/150‑1 (MQ=255)
                                                                                                                                     ctctTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                 <  2:1718083/150‑1 (MQ=255)
                                                                                                                                        ttACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAgg              >  2:783276/1‑150 (MQ=255)
                                                                                                                                          aCAGGGTGGCTAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGaa            <  2:756545/150‑1 (MQ=255)
                                                                                                                                           cAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGaaa           <  1:1015459/150‑1 (MQ=255)
                                                                                                                                            aGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGaaaa          <  1:287804/150‑1 (MQ=255)
                                                                                                                                              ggTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGc        >  1:2701758/1‑150 (MQ=255)
                                                                                                                                               gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGcc       <  2:4382759/150‑1 (MQ=255)
                                                                                                                                                 ggCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGt     <  1:1736006/150‑1 (MQ=255)
                                                                                                                                                  gCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCCGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTg    >  1:1814326/1‑150 (MQ=255)
                                                                                                                                                   cGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGc   >  2:1256413/1‑150 (MQ=255)
                                                                                                                                                    gAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCg  <  1:1632772/150‑1 (MQ=255)
                                                                                                                                                    gAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCg  <  1:4548200/150‑1 (MQ=255)
                                                                                                                                                    gAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCg  >  2:2172055/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCG  >  CP009273/4516586‑4516883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑