Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 486,169 A→T 6.9% F34I (TTC→ATC)  priC ← primosomal replication protein N''

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273486,1690AT 6.9% 801.7 / 38.5 233F34I (TTC→ATC) priCprimosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base A (109/108);  new base T (9/7);  total (118/115)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 236 total aligned reads displayed.

AGTTGTGCCGCCAGATGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGCCACTCT  >  CP009273/486021‑486317
                                                                                                                                                    |                                                                                                                                                    
    gtgCCGCCAGATGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAg                                                                                                                                                 >  1:3406436/1‑150 (MQ=255)
         gccAGATGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACtt                                                                                                                                            >  1:3845428/1‑150 (MQ=255)
          ccAGATGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTa                                                                                                                                           <  2:1637592/150‑1 (MQ=255)
           cAGATGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAg                                                                                                                                          <  1:4072392/150‑1 (MQ=255)
              aTGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGt                                                                                                                                       <  2:2286508/150‑1 (MQ=255)
                   ccGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGa                                                                                                                                  >  1:4947549/1‑150 (MQ=255)
                   ccGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGa                                                                                                                                  <  2:3852770/150‑1 (MQ=255)
                       cagccagGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACtg                                                                                                                              >  2:4836055/1‑150 (MQ=255)
                       cagccagGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACtg                                                                                                                              <  2:4743492/150‑1 (MQ=255)
                        agccagGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACtgt                                                                                                                             >  2:488757/1‑150 (MQ=255)
                        agccagGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACtgt                                                                                                                             <  2:4947520/150‑1 (MQ=255)
                        agccagGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACtgt                                                                                                                             >  2:3665242/1‑150 (MQ=255)
                           cagGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGac                                                                                                                          <  2:3046249/150‑1 (MQ=255)
                            agGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGaca                                                                                                                         >  1:3486692/1‑150 (MQ=255)
                            agGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGaca                                                                                                                         >  2:1987181/1‑150 (MQ=255)
                             ggCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGacac                                                                                                                        >  1:2714018/1‑150 (MQ=255)
                                cACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCgg                                                                                                                     >  2:4313800/1‑150 (MQ=255)
                                 aCTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCggg                                                                                                                    <  2:4673316/150‑1 (MQ=255)
                                  cTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCgggg                                                                                                                   >  2:699993/1‑150 (MQ=255)
                                    tGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGca                                                                                                                 <  2:4384765/150‑1 (MQ=255)
                                       ggCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACaa                                                                                                              >  2:5155298/1‑150 (MQ=255)
                                        gCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAAc                                                                                                             <  2:3814292/150‑1 (MQ=255)
                                          aGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGc                                                                                                           >  1:115304/1‑150 (MQ=255)
                                              gTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGAc                                                                                                       <  2:1453889/150‑1 (MQ=255)
                                              gTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGAc                                                                                                       <  2:1454559/150‑1 (MQ=255)
                                              gTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGAc                                                                                                       >  1:2488338/1‑150 (MQ=255)
                                              gTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGAc                                                                                                       <  1:2084812/150‑1 (MQ=255)
                                                      tCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTa                                                                                               <  1:922386/150‑1 (MQ=255)
                                                      tCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACCCAGCGTa                                                                                               >  2:4352385/1‑150 (MQ=255)
                                                        aaCTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGc                                                                                             <  2:4928759/150‑1 (MQ=255)
                                                         aCTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCg                                                                                            <  2:1803376/150‑1 (MQ=255)
                                                          cTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGa                                                                                           >  1:2919519/1‑150 (MQ=255)
                                                           tGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAg                                                                                          <  2:4222409/150‑1 (MQ=255)
                                                            gCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGc                                                                                         >  2:1182714/1‑150 (MQ=255)
                                                            gCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGc                                                                                         >  1:3216333/1‑150 (MQ=255)
                                                             cATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCt                                                                                        <  1:4971251/150‑1 (MQ=255)
                                                             cATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCt                                                                                        <  2:5089222/150‑1 (MQ=255)
                                                              aTGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTg                                                                                       >  1:4825215/1‑150 (MQ=255)
                                                                gACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTc                                                                                     >  2:2518895/1‑150 (MQ=255)
                                                                gACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTc                                                                                     <  1:1196675/150‑1 (MQ=255)
                                                                 aCGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTcc                                                                                    <  2:2665294/150‑1 (MQ=255)
                                                                  cGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCt                                                                                   <  2:1231203/150‑1 (MQ=255)
                                                                   gAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGACCTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCtt                                                                                  >  2:4781072/1‑150 (MQ=255)
                                                                   gAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCtt                                                                                  <  1:2238069/150‑1 (MQ=255)
                                                                              aGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTcc                                                                       >  1:2914042/1‑150 (MQ=255)
                                                                              aGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTcc                                                                       <  1:3588616/150‑1 (MQ=255)
                                                                                aTTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCcag                                                                     >  1:1459181/1‑150 (MQ=255)
                                                                                  tATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCcagca                                                                   >  1:1003789/1‑150 (MQ=255)
                                                                                   aTCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCcagcag                                                                  <  2:3515662/150‑1 (MQ=255)
                                                                                     cgcccgccTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCcagcagca                                                                >  2:1705259/1‑150 (MQ=255)
                                                                                        ccgccTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAggg                                                             >  2:115406/1‑150 (MQ=255)
                                                                                         cgccTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGc                                                            >  1:1503602/1‑150 (MQ=255)
                                                                                          gccTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCg                                                           >  2:4148164/1‑150 (MQ=255)
                                                                                          gccTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCg                                                           <  2:4825215/150‑1 (MQ=255)
                                                                                           ccTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCgg                                                          <  1:2300088/150‑1 (MQ=255)
                                                                                            cTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGt                                                         <  2:2728235/150‑1 (MQ=255)
                                                                                            cTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGAAGGGCGGt                                                         >  1:2665096/1‑150 (MQ=255)
                                                                                             tcgtcgAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGtt                                                        <  1:4511863/150‑1 (MQ=255)
                                                                                               gtcgAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGtttt                                                      >  2:4459377/1‑150 (MQ=255)
                                                                                               gtcgAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGtttt                                                      <  1:819373/150‑1 (MQ=255)
                                                                                                  gagaCAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCAc                                                   <  2:2548402/150‑1 (MQ=255)
                                                                                                   agaCAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACg                                                  >  2:409464/1‑150 (MQ=255)
                                                                                                   agaCAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACg                                                  <  1:1640099/150‑1 (MQ=255)
                                                                                                   agaCAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACg                                                  >  1:927143/1‑150 (MQ=255)
                                                                                                    gaCAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGa                                                 >  1:3888514/1‑150 (MQ=255)
                                                                                                     aCAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGAt                                                <  2:1693331/150‑1 (MQ=255)
                                                                                                      cAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATa                                               >  2:2125074/1‑150 (MQ=255)
                                                                                                      cAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATa                                               <  1:1730844/150‑1 (MQ=255)
                                                                                                       aaGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATaa                                              >  2:385704/1‑150 (MQ=255)
                                                                                                            tGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCt                                         <  1:1487743/150‑1 (MQ=255)
                                                                                                             gTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTc                                        <  1:3174658/150‑1 (MQ=255)
                                                                                                              tAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCa                                       <  2:454754/150‑1 (MQ=255)
                                                                                                               aGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCaa                                      >  2:4451043/1‑150 (MQ=255)
                                                                                                                    tgtCGCACGAGTCTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGaaa                                 >  2:1657616/1‑150 (MQ=255)
                                                                                                                      tCGCACGAGTCTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAAt                               <  1:1792201/150‑1 (MQ=255)
                                                                                                                      tCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAAt                               <  2:1601880/150‑1 (MQ=255)
                                                                                                                         cACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGAt                            <  1:982643/150‑1 (MQ=255)
                                                                                                                             aGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAAtt                        >  2:5080885/1‑150 (MQ=255)
                                                                                                                             aGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAAtt                        <  1:3665242/150‑1 (MQ=255)
                                                                                                                              gTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTg                       <  2:1717727/150‑1 (MQ=255)
                                                                                                                               tCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGt                      <  2:3152296/150‑1 (MQ=255)
                                                                                                                               tCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGt                      >  1:4687679/1‑150 (MQ=255)
                                                                                                                                cTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGtt                     <  1:4264643/150‑1 (MQ=255)
                                                                                                                                cTGAAAAAGATGCCTGTCGATACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGtt                     <  1:2526350/150‑1 (MQ=255)
                                                                                                                                cTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGtt                     >  1:2504376/1‑150 (MQ=255)
                                                                                                                                cTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGtt                     <  2:1582748/150‑1 (MQ=255)
                                                                                                                                  gAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTAt                   >  1:2858590/1‑150 (MQ=255)
                                                                                                                                   aaaaaGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATg                  <  1:4167352/150‑1 (MQ=255)
                                                                                                                                      aaGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTa               >  2:5132587/1‑150 (MQ=255)
                                                                                                                                       aGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTaa              >  1:43454/1‑150 (MQ=255)
                                                                                                                                        gATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTaaa             >  1:3411756/1‑150 (MQ=255)
                                                                                                                                         aTGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAg            <  1:699993/150‑1 (MQ=255)
                                                                                                                                          tGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGt           >  1:5099748/1‑150 (MQ=255)
                                                                                                                                           gCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTa          >  1:98470/1‑150 (MQ=255)
                                                                                                                                             cTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGc        >  2:2391098/1‑150 (MQ=255)
                                                                                                                                              tGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGcc       <  1:305334/150‑1 (MQ=255)
                                                                                                                                               gTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGCCa      >  1:2058321/1‑150 (MQ=255)
                                                                                                                                                tCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGCCAc     <  1:710844/150‑1 (MQ=255)
                                                                                                                                                 cGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGCCAct    <  1:1629954/150‑1 (MQ=255)
                                                                                                                                                   aaaCGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGCCActct  >  1:4637775/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGTTGTGCCGCCAGATGTTCCGCCAGCCAGGCCACTTGCGGCAGCTGTTGCTGCTCAACTGCATGACGAAGCGCAGCCAGATTATCGCCCGCCTCGTCGAGACAAGCCTGTAGTGTTGTCGCACGAGTCTGAAAAAGATGCCTGTCGAAACGAGCACTTAGCGTGGCGAACTGTGACACCGGGGCACAACGCTGACGCAGCGTAGCGAGCTGTCCTTCCAGTTTTTCCAGCAGCAGGGCGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAGTAGCCACTCT  >  CP009273/486021‑486317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑