Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 507,331 G→A 17.3% D79N (GAT→AAT)  glsA → glutaminase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273507,3310GA17.3% 657.4 / 116.0 271D79N (GAT→AAT) glsAglutaminase 1
Reads supporting (aligned to +/- strand):  ref base G (109/115);  new base A (21/26);  total (130/141)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

ACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAGCCTGATTAAC  >  CP009273/507182‑507480
                                                                                                                                                     |                                                                                                                                                     
aCAGTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTagaag                                                                                                                                                       <  1:2356845/150‑1 (MQ=255)
 cATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTagaaga                                                                                                                                                      <  1:3839957/150‑1 (MQ=255)
 cATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTagaaga                                                                                                                                                      >  2:4700170/1‑150 (MQ=255)
  agtCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGAt                                                                                                                                                     <  2:518621/148‑1 (MQ=255)
  aTTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGAt                                                                                                                                                     <  1:2803085/150‑1 (MQ=255)
  aTTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGAt                                                                                                                                                     <  2:2715021/150‑1 (MQ=255)
  aTTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGAt                                                                                                                                                     >  2:2640354/1‑150 (MQ=255)
   ttCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATg                                                                                                                                                    >  1:4320364/1‑150 (MQ=255)
     cccTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTc                                                                                                                                                  >  1:927786/1‑150 (MQ=255)
     cccTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTc                                                                                                                                                  >  2:1764344/1‑150 (MQ=255)
       cTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCgg                                                                                                                                                >  1:1174993/1‑150 (MQ=255)
        tttCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGc                                                                                                                                               >  2:1605632/1‑150 (MQ=255)
          tCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATGGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGccc                                                                                                                                             <  1:560209/150‑1 (MQ=255)
           cTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCg                                                                                                                                            >  2:3816249/1‑150 (MQ=255)
           cTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCg                                                                                                                                            >  1:2090247/1‑150 (MQ=255)
             ggCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCa                                                                                                                                          <  2:1284001/150‑1 (MQ=255)
             ggCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCa                                                                                                                                          >  1:537836/1‑150 (MQ=255)
             ggCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCa                                                                                                                                          >  1:463023/1‑150 (MQ=255)
             ggCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCa                                                                                                                                          >  2:3432790/1‑150 (MQ=255)
                gAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGc                                                                                                                                       >  2:2918243/1‑150 (MQ=255)
                gAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGc                                                                                                                                       >  1:3996165/1‑150 (MQ=255)
                  aTGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCgg                                                                                                                                     >  2:2816689/1‑150 (MQ=255)
                     tACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTAc                                                                                                                                  <  1:4866897/150‑1 (MQ=255)
                      aCCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACa                                                                                                                                 <  2:4600465/150‑1 (MQ=255)
                      aCCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACa                                                                                                                                 <  1:1088169/150‑1 (MQ=255)
                            tCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACaa                                                                                                                           >  1:626291/1‑150 (MQ=255)
                                cTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAAtt                                                                                                                       <  1:4049458/150‑1 (MQ=255)
                                 tGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTg                                                                                                                      >  2:457601/1‑150 (MQ=255)
                                  ggcggcAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTgg                                                                                                                     <  1:2783461/150‑1 (MQ=255)
                                   gcggcAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGgc                                                                                                                    <  2:1572437/150‑1 (MQ=255)
                                     ggcAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGgcgc                                                                                                                  >  2:3298004/1‑150 (MQ=255)
                                      gcAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCt                                                                                                                 <  2:1023708/150‑1 (MQ=255)
                                        aGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGa                                                                                                               >  2:3145561/1‑150 (MQ=255)
                                        aGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGa                                                                                                               >  2:3454334/1‑150 (MQ=255)
                                         gTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGAc                                                                                                              <  1:2972201/150‑1 (MQ=255)
                                            gCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCg                                                                                                           >  1:2496238/1‑150 (MQ=255)
                                               aTCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGAcc                                                                                                        <  2:1723727/150‑1 (MQ=255)
                                                tCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCg                                                                                                       >  1:3807238/1‑150 (MQ=255)
                                                  gTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGa                                                                                                     >  1:531435/1‑150 (MQ=255)
                                                  gTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGa                                                                                                     <  2:4100183/150‑1 (MQ=255)
                                                  gTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGa                                                                                                     >  2:5147612/1‑150 (MQ=255)
                                                    gACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGAtt                                                                                                   >  1:5050610/1‑150 (MQ=255)
                                                    gACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGAtt                                                                                                   <  1:3329526/150‑1 (MQ=255)
                                                       cTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGcc                                                                                                <  2:189284/150‑1 (MQ=255)
                                                           gATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCttt                                                                                            <  2:3931330/150‑1 (MQ=255)
                                                           gATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCttt                                                                                            <  1:1643139/150‑1 (MQ=255)
                                                            aTGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTa                                                                                           <  1:2959460/150‑1 (MQ=255)
                                                               gCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACt                                                                                        >  1:4390457/1‑150 (MQ=255)
                                                                cAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTc                                                                                       <  2:4987499/150‑1 (MQ=255)
                                                                   cGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGt                                                                                    <  2:4771859/150‑1 (MQ=255)
                                                                    gTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGtt                                                                                   <  1:145768/150‑1 (MQ=255)
                                                                    gTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGtt                                                                                   >  1:1888577/1‑150 (MQ=255)
                                                                    gTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGtt                                                                                   <  2:1856573/150‑1 (MQ=255)
                                                                      cTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTAt                                                                                 <  2:2283638/150‑1 (MQ=255)
                                                                       tataGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATc                                                                                >  1:4531643/1‑150 (MQ=255)
                                                                       tataGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATc                                                                                <  2:1102007/150‑1 (MQ=255)
                                                                        ataGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCg                                                                               <  2:4659606/150‑1 (MQ=255)
                                                                            tGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCtt                                                                           >  2:4507219/1‑150 (MQ=255)
                                                                            tGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCtt                                                                           <  1:2435163/150‑1 (MQ=255)
                                                                             gCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTa                                                                          <  1:4698854/150‑1 (MQ=255)
                                                                                  tGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGtt                                                                     <  2:3762673/150‑1 (MQ=255)
                                                                                  tGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGtt                                                                     >  1:793920/1‑150 (MQ=255)
                                                                                   gACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTATCTCAGTTATCGCCTTAGAGTTg                                                                    >  1:3931130/1‑150 (MQ=255)
                                                                                   gACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTg                                                                    <  2:4214415/150‑1 (MQ=255)
                                                                                    aCAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGc                                                                   <  2:603812/150‑1 (MQ=255)
                                                                                        tGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATgg                                                               >  2:39149/1‑150 (MQ=255)
                                                                                         gATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATggc                                                              <  1:699360/150‑1 (MQ=255)
                                                                                          aTTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATggcg                                                             >  2:3881677/1‑150 (MQ=255)
                                                                                            tACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCAGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATggcggc                                                           >  2:3081250/1‑150 (MQ=255)
                                                                                                 cTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAAcc                                                      >  1:185551/1‑150 (MQ=255)
                                                                                                 cTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAAcc                                                      >  1:4822002/1‑150 (MQ=255)
                                                                                                    tGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCt                                                   >  2:2972388/1‑150 (MQ=255)
                                                                                                     gCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCtt                                                  >  1:3177951/1‑150 (MQ=255)
                                                                                                       aCTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTc                                                <  1:3516672/150‑1 (MQ=255)
                                                                                                       aCTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTc                                                <  2:3985439/150‑1 (MQ=255)
                                                                                                       aCTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTc                                                <  2:1464096/150‑1 (MQ=255)
                                                                                                       aCTGGAATCCAGCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAATATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTc                                                <  2:7964/150‑1 (MQ=255)
                                                                                                          ggAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGcc                                             >  1:1032851/1‑150 (MQ=255)
                                                                                                             aTCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACt                                          <  1:2507521/150‑1 (MQ=255)
                                                                                                                  tctcGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTaa                                     <  1:1365797/150‑1 (MQ=255)
                                                                                                                   ctcGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTaaa                                    >  1:2218588/1‑150 (MQ=255)
                                                                                                                    tcGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAAt                                   <  2:2369226/150‑1 (MQ=255)
                                                                                                                      gAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGc                                 >  2:50979/1‑150 (MQ=255)
                                                                                                                       aaaGTCTGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGACTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCt                                >  1:1973087/1‑150 (MQ=255)
                                                                                                                        aaGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTg                               <  2:938805/150‑1 (MQ=255)
                                                                                                                             tGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCt                          <  2:693949/150‑1 (MQ=255)
                                                                                                                             tGTACGTTAGCCCTTGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCt                          <  2:2961035/150‑1 (MQ=255)
                                                                                                                               tACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTAt                        <  2:4399679/150‑1 (MQ=255)
                                                                                                                               tACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTAt                        <  2:4806838/150‑1 (MQ=255)
                                                                                                                               tACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTAt                        <  1:1590321/150‑1 (MQ=255)
                                                                                                                                    tAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGcca                   >  2:2010233/1‑150 (MQ=255)
                                                                                                                                      gCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGccacc                 <  2:638487/150‑1 (MQ=255)
                                                                                                                                       cccTTGCGATAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGccacca                <  2:2041737/150‑1 (MQ=255)
                                                                                                                                         cTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGccaccacc              >  1:3580654/1‑150 (MQ=255)
                                                                                                                                          ttGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGccaccacca             >  1:323163/1‑150 (MQ=255)
                                                                                                                                           tGCGTTAGAAAATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAg            <  2:866151/150‑1 (MQ=255)
                                                                                                                                                 agaagaTGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAGCCTGAt      <  2:1356819/150‑1 (MQ=255)
                                                                                                                                                   aagaTGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAGCCTGATTa    >  1:2244134/1‑150 (MQ=255)
                                                                                                                                                     gaTGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAGCCTGATTAAc  <  2:218423/150‑1 (MQ=255)
                                                                                                                                                     gaTGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAGCCTGATTAAc  <  1:4961990/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCACTGGTAAATGCTGGCGCTATTGCCACCACCAGCCTGATTAAC  >  CP009273/507182‑507480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑