Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 708,114 C→A 5.0% D49Y (GAT→TAT)  ybfF ← acyl‑CoA esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273708,1140CA 5.0% 990.1 / 23.5 257D49Y (GAT→TAT) ybfFacyl‑CoA esterase
Reads supporting (aligned to +/- strand):  ref base C (120/124);  new base A (9/4);  total (129/128)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 257 total aligned reads displayed.

TGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCA  >  CP009273/707968‑708262
                                                                                                                                                  |                                                                                                                                                    
tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAc                                                                                                                                                   <  1:502268/150‑1 (MQ=255)
 gCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAcc                                                                                                                                                  >  1:1811895/1‑150 (MQ=255)
 gCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAcc                                                                                                                                                  <  1:3677771/150‑1 (MQ=255)
 gCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAcc                                                                                                                                                  <  1:345377/150‑1 (MQ=255)
  ccATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCATTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCt                                                                                                                                                 <  1:2343091/150‑1 (MQ=255)
      tACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgat                                                                                                                                             >  1:1814290/1‑150 (MQ=255)
      tACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgat                                                                                                                                             >  1:2051892/1‑150 (MQ=255)
       aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgatg                                                                                                                                            <  2:3093161/150‑1 (MQ=255)
        ccGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgatga                                                                                                                                           >  2:2710445/1‑150 (MQ=255)
        ccGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATAAACCTGgatga                                                                                                                                           >  2:2909038/1‑150 (MQ=255)
           cTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGatat                                                                                                                                        <  2:356172/150‑1 (MQ=255)
           cTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCACAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGatat                                                                                                                                        >  1:96261/1‑150 (MQ=255)
            tttACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATAtt                                                                                                                                       <  2:2802101/150‑1 (MQ=255)
                  gCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATc                                                                                                                                 >  1:3227574/1‑150 (MQ=255)
                  gCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATc                                                                                                                                 <  1:4095273/150‑1 (MQ=255)
                    ccATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGt                                                                                                                               >  1:3047429/1‑150 (MQ=255)
                    ccATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGt                                                                                                                               <  2:67355/150‑1 (MQ=255)
                     cATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGtt                                                                                                                              <  1:4676590/150‑1 (MQ=255)
                      aTGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGttt                                                                                                                             >  1:2742213/1‑150 (MQ=255)
                      aTGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGttt                                                                                                                             >  1:2743043/1‑150 (MQ=255)
                      aTGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGttt                                                                                                                             >  1:692730/1‑150 (MQ=255)
                      aTGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGttt                                                                                                                             <  2:3339124/150‑1 (MQ=255)
                      aTGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGttt                                                                                                                             <  2:1700901/150‑1 (MQ=255)
                       tGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTa                                                                                                                            >  1:2463792/1‑150 (MQ=255)
                        ggAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTAc                                                                                                                           >  2:4698665/1‑150 (MQ=255)
                           gTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATGGTGATCGTTTACCAg                                                                                                                        >  2:4115055/1‑150 (MQ=255)
                             gACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGAt                                                                                                                      <  2:4647976/150‑1 (MQ=255)
                             gACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGAt                                                                                                                      <  2:2267747/150‑1 (MQ=255)
                              aCCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATc                                                                                                                     >  2:1268072/1‑150 (MQ=255)
                              aCCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATc                                                                                                                     <  1:2277719/150‑1 (MQ=255)
                              aCCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATc                                                                                                                     >  2:3265000/1‑150 (MQ=255)
                               ccGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGGAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATcg                                                                                                                    <  2:191248/150‑1 (MQ=255)
                               ccGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATcg                                                                                                                    <  2:190191/150‑1 (MQ=255)
                               ccGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATcg                                                                                                                    <  2:190219/150‑1 (MQ=255)
                                 gATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATcgcg                                                                                                                  >  2:687816/1‑150 (MQ=255)
                                     aaTGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGcc                                                                                                              <  1:4246621/150‑1 (MQ=255)
                                      aTGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCa                                                                                                             <  1:489156/150‑1 (MQ=255)
                                        gTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGt                                                                                                           >  2:430016/1‑150 (MQ=255)
                                        gTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGt                                                                                                           >  2:1235900/1‑150 (MQ=255)
                                           gCTTTGTCGATCTGCTGTGCATCCAGAGTACCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACg                                                                                                        >  1:1275156/1‑150 (MQ=255)
                                              ttGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCa                                                                                                     <  2:750144/150‑1 (MQ=255)
                                                gTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAg                                                                                                   <  2:3503403/150‑1 (MQ=255)
                                                 tCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAgg                                                                                                  >  2:2613435/1‑150 (MQ=255)
                                                      ctgctgTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTc                                                                                             <  1:1450437/150‑1 (MQ=255)
                                                       tgctgTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCg                                                                                            >  2:194446/1‑150 (MQ=255)
                                                                       tATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGAc                                                                            <  2:1120318/150‑1 (MQ=255)
                                                                       tATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGAc                                                                            >  2:4749556/1‑150 (MQ=255)
                                                                        aTCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGAcc                                                                           <  1:4328527/150‑1 (MQ=255)
                                                                        aTCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGAcc                                                                           >  2:1991049/1‑150 (MQ=255)
                                                                         tCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCg                                                                          >  1:255646/1‑150 (MQ=255)
                                                                           aacaaGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTg                                                                        <  1:4390336/150‑1 (MQ=255)
                                                                             caaGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGa                                                                      >  2:2965313/1‑150 (MQ=255)
                                                                                gATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACaa                                                                   <  2:4293177/150‑1 (MQ=255)
                                                                                 aTCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTTTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAg                                                                  <  2:2477412/150‑1 (MQ=255)
                                                                                 aTCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAg                                                                  <  2:103720/150‑1 (MQ=255)
                                                                                  tCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGa                                                                 <  2:1704254/150‑1 (MQ=255)
                                                                                   ccTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGaa                                                                >  1:4647081/1‑150 (MQ=255)
                                                                                    cTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAAc                                                               <  2:2683443/150‑1 (MQ=255)
                                                                                    cTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAAc                                                               <  2:4483999/150‑1 (MQ=255)
                                                                                    cTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAAc                                                               <  1:1061649/150‑1 (MQ=255)
                                                                                     tGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACg                                                              >  2:2366152/1‑150 (MQ=255)
                                                                                       cgcCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGAt                                                            <  2:864445/150‑1 (MQ=255)
                                                                                          cATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATggg                                                         <  1:3611646/150‑1 (MQ=255)
                                                                                              gCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGaa                                                     <  1:4486499/150‑1 (MQ=255)
                                                                                               ccGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAat                                                    <  1:1400192/150‑1 (MQ=255)
                                                                                               ccGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAat                                                    <  1:3963353/150‑1 (MQ=255)
                                                                                                  ggTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAattat                                                 >  2:3942496/1‑150 (MQ=255)
                                                                                                    tAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATtg                                               >  1:2467661/1‑150 (MQ=255)
                                                                                                     aaTTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATtgt                                              >  2:3210782/1‑150 (MQ=255)
                                                                                                     aaTTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAAGTATtgt                                              >  1:1769975/1‑150 (MQ=255)
                                                                                                     aaTTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGAGGGGAGAATTATtgt                                              >  2:3853630/1‑150 (MQ=255)
                                                                                                        tCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCt                                           >  2:4167264/1‑150 (MQ=255)
                                                                                                        tCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCt                                           >  1:2155543/1‑150 (MQ=255)
                                                                                                         cATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTg                                          <  2:3129928/150‑1 (MQ=255)
                                                                                                            tACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGtt                                       <  1:834334/150‑1 (MQ=255)
                                                                                                              ccGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGtttt                                     >  1:3820674/1‑150 (MQ=255)
                                                                                                                ggATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGACTTATTGTGCTGGTTTtgt                                   >  2:4577949/1‑150 (MQ=255)
                                                                                                                ggATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTtgt                                   >  1:3125492/1‑150 (MQ=255)
                                                                                                                 gATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTtgtg                                  <  2:304944/150‑1 (MQ=255)
                                                                                                                 gATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTtgtg                                  <  2:1498793/150‑1 (MQ=255)
                                                                                                                      ctcGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGtt                             <  2:3827202/150‑1 (MQ=255)
                                                                                                                        cGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTg                           <  2:2051897/150‑1 (MQ=255)
                                                                                                                        cGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTg                           <  2:1814290/150‑1 (MQ=255)
                                                                                                                           tGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTgcgc                        <  2:3741794/150‑1 (MQ=255)
                                                                                                                           tGAAAGACCGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTgcgc                        >  2:3630148/1‑150 (MQ=255)
                                                                                                                            gAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTgcgcg                       <  1:889918/150‑1 (MQ=255)
                                                                                                                               aGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGACTTATTGTGCTGGTTTTGTGCAGTTTGCGCGCgg                    >  2:1799171/1‑150 (MQ=255)
                                                                                                                               aGACCGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCgg                    >  1:4160809/1‑150 (MQ=255)
                                                                                                                                 aCCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGat                  <  1:2965313/150‑1 (MQ=255)
                                                                                                                                  ccGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAAGGATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGatc                 >  2:1356073/1‑149 (MQ=255)
                                                                                                                                   cGTGGTTACGCATATAAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGatat                <  1:4588/150‑1 (MQ=255)
                                                                                                                                     tGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTc              <  1:1582520/150‑1 (MQ=255)
                                                                                                                                       gTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCaa            <  1:636865/150‑1 (MQ=255)
                                                                                                                                         tACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAAtt          <  1:1992620/150‑1 (MQ=255)
                                                                                                                                           cGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTc        >  2:766932/1‑150 (MQ=255)
                                                                                                                                           cGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTc        <  2:2338748/150‑1 (MQ=255)
                                                                                                                                             cATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCAt      >  2:472152/1‑150 (MQ=255)
                                                                                                                                              atatCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATg     >  1:4697667/1‑150 (MQ=255)
                                                                                                                                                atCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGc   >  2:4421308/1‑150 (MQ=255)
                                                                                                                                                atCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGc   <  2:1625208/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCA  >  CP009273/707968‑708262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑