Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 764,517 G→A 5.2% D362N (GAC→AAC)  mngB → alpha‑mannosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273764,5170GA 5.2% 832.3 / 21.7 231D362N (GAC→AAC) mngBalpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base G (121/98);  new base A (4/8);  total (125/106)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 231 total aligned reads displayed.

GGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCA  >  CP009273/764369‑764665
                                                                                                                                                    |                                                                                                                                                    
 gCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGAc                                                                                                                                                    <  2:4258635/150‑1 (MQ=255)
    tGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATg                                                                                                                                                 <  2:2065328/150‑1 (MQ=255)
      gACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGc                                                                                                                                               >  2:261428/1‑150 (MQ=255)
       aCGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCg                                                                                                                                              <  1:3831578/150‑1 (MQ=255)
       aCGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCg                                                                                                                                              >  2:3467226/1‑150 (MQ=255)
       aCGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCg                                                                                                                                              <  1:3120406/150‑1 (MQ=255)
       aCGTTGGGTTTTGAATACCACCACGGATTGCTGGAGAAAATTTGGAAATAGATCTTAAAAAATCATGACCACGACAGAATCGGCTGCTGATGTATTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCg                                                                                                                                              >  2:1314233/1‑150 (MQ=255)
        cGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCgg                                                                                                                                             <  2:1965721/150‑1 (MQ=255)
          ttGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGAt                                                                                                                                           >  2:1013884/1‑150 (MQ=255)
          ttGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGAt                                                                                                                                           >  2:734556/1‑150 (MQ=255)
          ttGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGAt                                                                                                                                           <  2:620831/150‑1 (MQ=255)
          ttGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGAt                                                                                                                                           >  2:3276359/1‑150 (MQ=255)
          ttGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAAACATGGCGGAt                                                                                                                                           >  2:751681/1‑150 (MQ=255)
              tttttGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATc                                                                                                                                       <  2:3728880/149‑1 (MQ=255)
              gTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATc                                                                                                                                       <  2:2696042/150‑1 (MQ=255)
              gTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATc                                                                                                                                       <  2:3705461/150‑1 (MQ=255)
               ttttTAATACCACCACGGCTTGCTTTATAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCt                                                                                                                                      <  1:2215567/150‑1 (MQ=255)
               ttttGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCt                                                                                                                                      >  2:1817191/1‑150 (MQ=255)
               ttttGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCt                                                                                                                                      >  1:1342968/1‑150 (MQ=255)
                   gAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGAtt                                                                                                                                  <  2:151281/150‑1 (MQ=255)
                   gAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGAtt                                                                                                                                  <  2:1068687/150‑1 (MQ=255)
                    aaTACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTc                                                                                                                                 >  2:3825669/1‑150 (MQ=255)
                     aTACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCg                                                                                                                                <  2:520716/150‑1 (MQ=255)
                      tACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGt                                                                                                                               <  2:2690225/150‑1 (MQ=255)
                      tACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGt                                                                                                                               >  1:4405797/1‑150 (MQ=255)
                      tACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGt                                                                                                                               >  2:3873692/1‑150 (MQ=255)
                      tACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGt                                                                                                                               <  1:3332629/150‑1 (MQ=255)
                           ccacGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTa                                                                                                                          <  1:3736867/150‑1 (MQ=255)
                           ccacGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATAATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTa                                                                                                                          <  1:2743830/150‑1 (MQ=255)
                            cacGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTAc                                                                                                                         >  1:1817461/1‑150 (MQ=255)
                            cacGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAAACATGGCGGATAATCTGATTCGTTTCTAc                                                                                                                         <  2:4348148/150‑1 (MQ=255)
                               ggCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATg                                                                                                                      >  1:2019596/1‑150 (MQ=255)
                                gCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATgc                                                                                                                     >  2:2156313/1‑150 (MQ=255)
                                 cTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATgcg                                                                                                                    <  2:3201470/150‑1 (MQ=255)
                                     cTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCaaa                                                                                                                <  1:4187212/150‑1 (MQ=255)
                                     cTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCaaa                                                                                                                >  1:423589/1‑150 (MQ=255)
                                     cTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCaaa                                                                                                                >  1:235198/1‑150 (MQ=255)
                                          gaAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGc                                                                                                           >  2:2037576/1‑150 (MQ=255)
                                           aaaaTGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGcc                                                                                                          <  1:751383/150‑1 (MQ=255)
                                              aTGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGac                                                                                                       >  1:3072703/1‑150 (MQ=255)
                                                 tgGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGacaac                                                                                                    >  2:2459593/1‑150 (MQ=255)
                                                  ggAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGacaaca                                                                                                   <  1:4003096/150‑1 (MQ=255)
                                                   gAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACAt                                                                                                  <  1:3984133/150‑1 (MQ=255)
                                                    aaaGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATg                                                                                                 <  1:1648816/150‑1 (MQ=255)
                                                    aaaGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATg                                                                                                 <  1:2531086/150‑1 (MQ=255)
                                                     aaGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGc                                                                                                <  2:4720603/150‑1 (MQ=255)
                                                          aTCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCag                                                                                           <  2:4040480/150‑1 (MQ=255)
                                                            cTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCagag                                                                                         >  1:2185870/1‑150 (MQ=255)
                                                             ttAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGc                                                                                        >  1:901676/1‑150 (MQ=255)
                                                              tAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCg                                                                                       <  1:3169395/150‑1 (MQ=255)
                                                               aaaaaaTCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCACAATTGCCGACAACATGCCGCAGAGCGa                                                                                      >  2:2004620/1‑150 (MQ=255)
                                                               aaaaaaTCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGa                                                                                      >  2:4252086/1‑150 (MQ=255)
                                                                  aaaTCATGCCCACTACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGc                                                                                   >  1:583090/1‑150 (MQ=255)
                                                                   aaTCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGcc                                                                                  <  1:3579735/150‑1 (MQ=255)
                                                                       aTGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACa                                                                              >  1:123081/1‑150 (MQ=255)
                                                                       aTGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACa                                                                              >  2:1556018/1‑150 (MQ=255)
                                                                        tGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACaa                                                                             <  1:1065258/150‑1 (MQ=255)
                                                                          cccACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAAc                                                                           >  1:2427845/1‑150 (MQ=255)
                                                                          cccACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAAc                                                                           <  2:635931/150‑1 (MQ=255)
                                                                           ccACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACt                                                                          >  1:698043/1‑150 (MQ=255)
                                                                            cACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTc                                                                         >  1:3202613/1‑150 (MQ=255)
                                                                              cGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGt                                                                       <  1:2408706/150‑1 (MQ=255)
                                                                              cGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGt                                                                       >  1:4573910/1‑150 (MQ=255)
                                                                               gACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTc                                                                      >  1:2687022/1‑150 (MQ=255)
                                                                               gACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAAACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTc                                                                      >  2:3479376/1‑150 (MQ=255)
                                                                                 cAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCt                                                                    >  1:3902053/1‑150 (MQ=255)
                                                                                    tATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAAACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGtt                                                                 <  2:2949497/150‑1 (MQ=255)
                                                                                        ggCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAAc                                                             >  2:881542/1‑150 (MQ=255)
                                                                                          ctgctgctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCt                                                           <  2:3034861/150‑1 (MQ=255)
                                                                                           tgctgctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGTGACGCCGACAAACTCGTCCTGTTTAACCTg                                                          >  2:3937852/1‑150 (MQ=255)
                                                                                           tgctgctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTg                                                          >  2:4544747/1‑150 (MQ=255)
                                                                                             ctgctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGAt                                                        <  1:3486764/150‑1 (MQ=255)
                                                                                              tgctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATg                                                       <  2:1821523/150‑1 (MQ=255)
                                                                                                ctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGcc                                                     >  2:64420/1‑150 (MQ=255)
                                                                                                 tgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGccc                                                    >  1:428907/1‑150 (MQ=255)
                                                                                                  gTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCt                                                   >  2:66945/1‑150 (MQ=255)
                                                                                                   tAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTg                                                  <  1:3058657/150‑1 (MQ=255)
                                                                                                    aGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACGTGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTgg                                                 >  2:1785497/1‑150 (MQ=255)
                                                                                                    aGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTgg                                                 <  2:3082521/150‑1 (MQ=255)
                                                                                                     gTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGc                                                <  1:3248505/150‑1 (MQ=255)
                                                                                                       gACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCcg                                              >  1:219916/1‑150 (MQ=255)
                                                                                                          aaaGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGt                                           >  1:3531605/1‑150 (MQ=255)
                                                                                                           aaGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTg                                          <  1:820145/150‑1 (MQ=255)
                                                                                                              ttCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaag                                       <  1:4326758/150‑1 (MQ=255)
                                                                                                              ttCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaag                                       <  2:3394774/150‑1 (MQ=255)
                                                                                                                      gAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATc                               >  1:171890/1‑150 (MQ=255)
                                                                                                                       aaaTCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCa                              <  2:3834523/150‑1 (MQ=255)
                                                                                                                       aaaTCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCa                              >  1:2377312/1‑150 (MQ=255)
                                                                                                                         aTCGTCGCCCGCTTCGAACTGGCTGAAAACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAac                            <  1:1147965/150‑1 (MQ=255)
                                                                                                                           cgtcgCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAacac                          <  1:590985/150‑1 (MQ=255)
                                                                                                                              cgCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAAcaccac                       <  2:1715625/150‑1 (MQ=255)
                                                                                                                              cgCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAAcaccac                       >  2:316306/1‑150 (MQ=255)
                                                                                                                               gCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACt                      >  1:4736383/1‑150 (MQ=255)
                                                                                                                                     ttCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCgg                >  2:2794540/1‑150 (MQ=255)
                                                                                                                                           cTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTgcgc          <  1:3180166/150‑1 (MQ=255)
                                                                                                                                              gCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgcc       >  2:665042/1‑150 (MQ=255)
                                                                                                                                               cTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgcca      <  2:2202066/150‑1 (MQ=255)
                                                                                                                                                tGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag     <  1:1757416/150‑1 (MQ=255)
                                                                                                                                                tGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag     >  1:1570329/1‑150 (MQ=255)
                                                                                                                                                   aGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccagcca  >  2:4186412/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCA  >  CP009273/764369‑764665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑