BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	776,694	T→A	5.3%	intergenic (+16/‑131)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	776,694	0	T	A	5.3%	598.9 / 5.8	210	intergenic (+16/‑131)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (99/93); new base A (11/4); total (113/97)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  CP009273/776545‑776843

 agCACCTCCCTTACAAGGA‑GGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAGTTTTAACAACCAAATCa                                                                                                                                                     >  1:837452/1‑150 (MQ=11)

   cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAg                                                                                                                                                    <  1:3313532/150‑1 (MQ=40)

   cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAg                                                                                                                                                    >  1:2214030/1‑150 (MQ=40)

   cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCCGTTTAAAATCAAACACAg                                                                                                                                                    >  1:1233924/1‑150 (MQ=11)

      cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATg                                                                                                                                                 >  2:2656352/1‑150 (MQ=255)

      cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCACATg                                                                                                                                                 >  2:2657920/1‑150 (MQ=255)

         ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTa                                                                                                                                              <  2:1579638/150‑1 (MQ=255)

         ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTTAACATCAAACTCAGCTGTTa                                                                                                                                              >  2:2439225/1‑150 (MQ=255)

            tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGc                                                                                                                                           <  1:4228503/150‑1 (MQ=255)

             aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCg                                                                                                                                          <  1:2900524/150‑1 (MQ=255)

               aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAATTTTAATAAATTGGTGGCAGGTTCGAATACATGACGACCCACCAGTTTTAACATAAAAAACAGATGTTAAGCGTg                                                                                                                                        >  1:1188272/1‑150 (MQ=11)

                  gaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAg                                                                                                                                     <  2:533102/150‑1 (MQ=255)

                  gaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAg                                                                                                                                     <  1:2233212/150‑1 (MQ=255)

                   aggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAgg                                                                                                                                    <  1:389452/150‑1 (MQ=255)

                   aggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAgg                                                                                                                                    <  2:2124667/150‑1 (MQ=255)

                   aggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAgg                                                                                                                                    <  1:391163/150‑1 (MQ=255)

                      gggTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATa                                                                                                                                 <  1:1991926/150‑1 (MQ=255)

                      gggTCGGCGGTTCGATCCAGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATa                                                                                                                                 <  2:1068590/150‑1 (MQ=255)

                         tCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACg                                                                                                                              <  2:2704653/150‑1 (MQ=255)

                         tCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAATCTGATGTTAAGCGTGAAGGATaaag                                                                                                                              >  2:2130030/1‑148 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCCGTTGGTAGAGCAGTTGTCTGTTAATCAATTGGTCGCAGGTTCGAATCCTGCACCACCCACCACTTTTAAAATCAAAAACAGTTGTTAATCGGTAAGGATAACGTTggg                                                                                                                         >  1:401364/1‑148 (MQ=11)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAATATAAGATGTTAAGCGTGAAGGATAACTTTgcg                                                                                                                         >  2:1234266/1‑150 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAATCGTGAAGGATAACGTTgcg                                                                                                                         >  1:2109595/1‑150 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         >  2:1687580/1‑150 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAACAAATGGTCGCAGGTTCGAATCCAGCACGACCCACCACTTTTAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         >  1:1704994/1‑150 (MQ=14)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGGTGGTAGAGCCGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                         >  1:3112891/1‑150 (MQ=255)

                               gTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAAAGTGTTGTTAAGCTGGAAGGATAGCGTTgcgc                                                                                                                        >  2:2108162/1‑150 (MQ=11)

                                ttCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGTGCAGTTGACTTTTTATCAATTGGTCGCAGGGTCGAATACGGCACGACCCACCCGTTTTAACCTCAAACGCAGATGTTAAGCGTGGAGGGTAACGGTGCGcc                                                                                                                       >  1:89989/1‑150 (MQ=14)

                                   gATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGc                                                                                                                    <  2:2230288/150‑1 (MQ=255)

                                    aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                                                                                                                   <  1:2650513/150‑1 (MQ=255)

                                     tCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCaa                                                                                                                  >  1:2333365/1‑150 (MQ=255)

                                          tcatcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTTGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCATTAGAGGTTGTTAAGCGTGTAGGGTAAAGTTGCGCCAGCaaacgcc                                                                                                             >  1:4507244/1‑145 (MQ=11)

                                           catcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGTGCTGTTGACTTTTAATCAATTGGTCGATGGTTCTATTCCTGCACGACCCACCAGTTTTAACATAAAACTCATATGTTAAGCGTGAAGTATAACGTTGCGCCAGCAACGGccc                                                                                                            <  2:623967/150‑1 (MQ=14)

                                            atcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCg                                                                                                           >  1:3936629/1‑150 (MQ=255)

                                              cacccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATACTGCACGACCCACCACTTTTAACCTCAAAAACGGTTGTTAAGCGTGAAGGAAAAAGATGAACCAGCAACTGCCCgga                                                                                                         >  2:3985453/1‑148 (MQ=14)

                                               acccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCTAATCCTGCACGACCCACCATTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAg                                                                                                        <  2:3000396/150‑1 (MQ=255)

                                               acccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACCGCCCGTAg                                                                                                        >  1:1087901/1‑150 (MQ=255)

                                                   accacTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTACCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg                                                                                                    <  2:2372601/150‑1 (MQ=255)

                                                     cacTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAACAAATTGTCGCAGGTTCGAATCCAGCACGACCCACCACTTTTAAAAACAAAATCAAATTTTAAGCGTGAAGGATAAACTTTCGCCAGCAACGGCCCGGAGGgcgag                                                                                                  >  1:542473/1‑150 (MQ=14)

                                                           ggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagc                                                                                            <  2:1013783/150‑1 (MQ=255)

                                                               gTTAGCTCAGTTGGTAGAGCTGTTTACTTTTAATCAATTGGTCGCAGGTCCGAATCCTGCACGACCCACCAGTTTTAACACCAAACTCAGATGTTAAGCGTGAAGGATAACGTGGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                        <  2:3241190/150‑1 (MQ=14)

                                                               gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAg                                                                                                                                                    <  2:287262/90‑1 (MQ=40)

                                                                       aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAAGTTAAGCGTGAAGGATAACGTTGCTCCAGCAACGGCACGTAGGGCGAGCGAAGCGAGTCATCCTGc                                                                                >  2:937529/1‑150 (MQ=255)

                                                                          tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACg                                                                             >  1:2950426/1‑150 (MQ=255)

                                                                          tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACg                                                                             >  2:913863/1‑150 (MQ=255)

                                                                          tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGAAGGGCGAGCGAAGCGAGTCAAACTGAACg                                                                             >  1:2950113/1‑150 (MQ=255)

                                                                                   tgTTTACTTTTAATCATTTGGTGGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa                                                                    <  2:1227193/149‑1 (MQ=255)

                                                                                   aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTc                                                                                                                                                      <  2:3544339/68‑1 (MQ=40)

                                                                                   aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTc                                                                                                                                                      >  1:3543231/1‑68 (MQ=40)

                                                                                       gaattttAAAAAATGGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAAATCAGATGTTAAGCGTGAAGTATAAGGTTGCGCCAGAAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCtt                                                                <  1:1834694/147‑1 (MQ=14)

                                                                                           tttAATCATTTGGTCGCTGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTATGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAg                                                            <  1:2732196/150‑1 (MQ=255)

                                                                                            ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCGTAAAGa                                                           >  2:3731142/1‑150 (MQ=255)

                                                                                              aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                         >  1:4626778/1‑150 (MQ=255)

                                                                                               aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTg                                                        >  2:263339/1‑150 (MQ=255)

                                                                                               aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTGAAAGATTg                                                        >  2:4512921/1‑150 (MQ=255)

                                                                                               aTCAATTGGTCGAAGGTTCGAATCCTGCACGACCCACCAGATTAAACATCAAACTAAGATGATAAGCGTGAAGGATAACGTTCCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGATTCATACAGAAAGACCCACCAATCTTAAAGAATg                                                        >  1:3852793/1‑150 (MQ=14)

                                                                                                tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                       >  1:624258/1‑150 (MQ=255)

                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGACGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGccc                                                    >  2:3484131/1‑150 (MQ=255)

                                                                                                    ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                   >  1:3606545/1‑150 (MQ=255)

                                                                                                     tGGTCGCTGGTGCGAATCCGGCACGACCCACCATTTTTAACATCAAACTCAGTTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCg                                                  <  2:3970567/150‑1 (MQ=17)

                                                                                                     tGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCg                                                  <  2:3402142/150‑1 (MQ=255)

                                                                                                        tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                               >  1:4401319/1‑150 (MQ=255)

                                                                                                            aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaa                                           <  2:2260071/150‑1 (MQ=255)

                                                                                                             ggTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCCGTTGTTAAGCGGGAAGGAAAACGTTGCGCAAGCACCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTACAGATACGCACCGAGAacaaa                                          >  1:1428790/1‑150 (MQ=14)

                                                                                                             ggTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCATATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaaa                                          <  2:436028/150‑1 (MQ=255)

                                                                                                             ggTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCTTCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaaa                                          >  2:3857064/1‑150 (MQ=255)

                                                                                                             ggTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTgaaaa                                          >  2:3533950/1‑150 (MQ=255)

                                                                                                              gTTCTAACCCTGCACGACCCACCATTTTTAACAACAACCTCATATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                         <  1:2656352/150‑1 (MQ=18)

                                                                                                               ttCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATc                                        >  2:4687595/1‑150 (MQ=255)

                                                                                                                  gAATCCTGCACGACCCACCAGTTTTACCATCAAACACAGATGTTAAGCGTCAATGATAACCTTGCTCCAGCAACGTCCCGTAGGGCGAGAGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     <  2:2540058/150‑1 (MQ=18)

                                                                                                                  gAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     >  1:1399279/1‑150 (MQ=255)

                                                                                                                  gAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     >  1:3525061/1‑150 (MQ=255)

                                                                                                                    aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                   <  2:3125351/150‑1 (MQ=255)

                                                                                                                      ccTGCACGACCCACAAGTTTTAAAAAAAACATCTTTTTTTATGCGTTAAGGATAAGGTTGCGCAAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAgg                                 <  1:3325514/150‑1 (MQ=21)

                                                                                                                       cTGCACGACCCACCATTTTTAACATCAAACTCAGATTTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                <  1:1551471/150‑1 (MQ=255)

                                                                                                                       cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                >  1:1965805/1‑150 (MQ=255)

                                                                                                                       cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                <  2:1778065/150‑1 (MQ=255)

                                                                                                                          cACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAac                             >  1:2401111/1‑150 (MQ=255)

                                                                                                                          cACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAac                             >  2:2004770/1‑150 (MQ=255)

                                                                                                                           aCGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAaca                            >  1:1351891/1‑150 (MQ=255)

                                                                                                                            cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                           >  1:2779279/1‑150 (MQ=255)

                                                                                                                            cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                           >  1:953953/1‑150 (MQ=255)

                                                                                                                             gACCCACAATTTTTAAAATCAAACTCAGATGTTAAGCGTGATGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAcc                          <  1:1171484/150‑1 (MQ=255)

                                                                                                                               cccACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCg                        <  2:2282316/150‑1 (MQ=255)

                                                                                                                                ccaccaGTTTTAACATCAAACTCAGTTGTTATGCGTGAAGGATAAGGTTGCGCCCGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                       <  2:837452/150‑1 (MQ=255)

                                                                                                                                  accaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                     <  2:2176768/150‑1 (MQ=255)

                                                                                                                                         ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt              >  2:952159/1‑150 (MQ=255)

                                                                                                                                          tAACATCAAACTCAGTTGTTAAGCGTGACGGATAACGGGGCGCCCGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt             <  1:1816295/150‑1 (MQ=25)

                                                                                                                                          tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt             >  1:739848/1‑150 (MQ=255)

                                                                                                                                           aaCATCAAACACAGATGTTACGCGTGAAGGATAACGTGGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTa            <  2:3316789/150‑1 (MQ=255)

                                                                                                                                              aacAAACTCATATGTTAAGCGTGAAGTATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt         <  1:647814/148‑1 (MQ=255)

                                                                                                                                                cAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa       >  2:2986007/1‑150 (MQ=255)

                                                                                                                                                   aCTCAGATTTTAAGCGTGAAGGATAAGGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    <  1:2968963/150‑1 (MQ=255)

                                                                                                                                                   aCTCAGATGTTAAGCGTGAAGGATAACTTGGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    <  2:1188272/150‑1 (MQ=255)

                                                                                                                                                   aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    >  1:428062/1‑150 (MQ=255)

                                                                                                                                                    cTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTg   >  1:1257811/1‑150 (MQ=255)

                                                                                                                                                     tCAGATTTTAAGCGTTAAGTATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  <  1:1685066/150‑1 (MQ=255)

                                                                                                                                                     tCAGATGTTACGCGTTAAGTATAAGGTTGCGCCACCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  <  1:1324794/150‑1 (MQ=37)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  <  2:1113306/150‑1 (MQ=255)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAACTTTCAGGTAACACCCGGATGGGTCGTTAGCTCAGTTgg  >  1:4307260/1‑150 (MQ=255)

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  CP009273/776545‑776843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`