Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 943,573 G→T 42.4% A297S (GCC→TCC)  ycaM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273943,5730GT42.4% 218.2 / inf 261A297S (GCC→TCC) ycaMputative transporter
Reads supporting (aligned to +/- strand):  ref base G (82/68);  new base T (57/54);  total (139/122)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 268 total aligned reads displayed.

CGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTATTTT  >  CP009273/943424‑943722
                                                                                                                                                     |                                                                                                                                                     
      gtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTg                                                                                                                                                 <  1:3859142/150‑1 (MQ=255)
      gtgCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTg                                                                                                                                                 >  1:3295616/1‑150 (MQ=255)
                gggCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCt                                                                                                                                       <  1:4160976/150‑1 (MQ=255)
                 ggCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTg                                                                                                                                      >  2:1074549/1‑150 (MQ=255)
                     cGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCTGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGAc                                                                                                                                  <  1:3066277/150‑1 (MQ=255)
                      gCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACa                                                                                                                                 >  1:3775568/1‑150 (MQ=255)
                       cTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACaa                                                                                                                                <  1:786108/150‑1 (MQ=255)
                        tGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAg                                                                                                                               >  1:716189/1‑150 (MQ=255)
                        tGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAg                                                                                                                               >  1:2107391/1‑150 (MQ=255)
                           cGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAg                                                                                                                            <  1:4189510/150‑1 (MQ=255)
                              tGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAgcgg                                                                                                                         >  1:1625081/1‑150 (MQ=255)
                                  gatgatGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCt                                                                                                                     >  2:525876/1‑150 (MQ=255)
                                  gatgatGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCt                                                                                                                     >  2:1813292/1‑150 (MQ=255)
                                   atgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTg                                                                                                                    >  1:4321638/1‑150 (MQ=255)
                                    tgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTgg                                                                                                                   >  1:143281/1‑150 (MQ=255)
                                    tgatgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTgg                                                                                                                   >  1:3092056/1‑150 (MQ=255)
                                      atgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTa                                                                                                                 >  1:1832780/1‑150 (MQ=255)
                                      atgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTa                                                                                                                 >  1:3428826/1‑150 (MQ=255)
                                       tgTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAt                                                                                                                <  1:2181897/150‑1 (MQ=255)
                                         tttGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtc                                                                                                              >  2:1413561/1‑150 (MQ=255)
                                           tGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                            <  2:3786231/150‑1 (MQ=255)
                                           tGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATtctc                                                                                                            >  2:2245984/1‑150 (MQ=255)
                                            gATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCg                                                                                                           >  2:4156628/1‑150 (MQ=255)
                                             aTTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGa                                                                                                          >  2:1454402/1‑150 (MQ=255)
                                              ttCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGAt                                                                                                         >  1:3172038/1‑150 (MQ=255)
                                               tCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGAtt                                                                                                        >  2:1147555/1‑150 (MQ=255)
                                                cgcgTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTg                                                                                                       <  2:2205129/150‑1 (MQ=255)
                                                           ccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCtt                                                                                            <  1:3647144/150‑1 (MQ=255)
                                                           ccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCtt                                                                                            >  2:2703421/1‑150 (MQ=255)
                                                           ccGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCtt                                                                                            <  1:1885199/150‑1 (MQ=255)
                                                            cGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTa                                                                                           >  2:4571562/1‑150 (MQ=255)
                                                             ggATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTaa                                                                                          >  2:2506752/1‑150 (MQ=255)
                                                                tGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGt                                                                                       <  2:4658962/150‑1 (MQ=255)
                                                                 gACTTAATGACCACCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTg                                                                                      <  1:2726684/150‑1 (MQ=255)
                                                                 gACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTg                                                                                      <  1:922511/150‑1 (MQ=255)
                                                                  aCTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGc                                                                                     >  1:1099552/1‑150 (MQ=255)
                                                                   cTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCt                                                                                    <  1:1729027/150‑1 (MQ=255)
                                                                    ttAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTa                                                                                   >  2:2477958/1‑150 (MQ=255)
                                                                       aTGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTa                                                                                <  2:3255629/150‑1 (MQ=255)
                                                                         gACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGGGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTAGTCTCGATTGATGCCCCGCTTAAAGTGCTATTAgg                                                                              >  1:3019098/1‑150 (MQ=255)
                                                                           ccACCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTg                                                                            <  1:3994093/150‑1 (MQ=255)
                                                                              aCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATg                                                                         >  2:3887892/1‑150 (MQ=255)
                                                                              aCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATg                                                                         >  2:3687018/1‑150 (MQ=255)
                                                                              aCGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATg                                                                         <  2:3213051/150‑1 (MQ=255)
                                                                                ggTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCt                                                                       >  2:767211/1‑150 (MQ=255)
                                                                                ggTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCt                                                                       <  2:4637704/150‑1 (MQ=255)
                                                                                 gTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACAGGGGTAATACTTTAATGGTGATTTACTCCATTGCGAAGACCCTGGGACAAGTAGCGGCGCGGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTg                                                                      <  2:3948512/150‑1 (MQ=255)
                                                                                  tCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGa                                                                     >  2:3508979/1‑150 (MQ=255)
                                                                                   cAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGAc                                                                    >  2:240979/1‑150 (MQ=255)
                                                                                   cAGTAATAAGCATTTCAGAAGCTGGGCGAGTATTACAACATTGGAAATACTTTAATGGTCATTTACGCCAATGCGAATACCCTGGGACCAGAAGCGGCTCTGGGAAACTCTATTGATGCCCCGCAAAAAGTGCGAATAGGTGTTGCTGAc                                                                    >  2:3589644/1‑150 (MQ=255)
                                                                                     gTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAg                                                                  <  2:2541840/150‑1 (MQ=255)
                                                                                        ttACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaa                                                               >  2:338951/1‑150 (MQ=255)
                                                                                        ttACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaa                                                               >  2:3034764/1‑150 (MQ=255)
                                                                                         tACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaaa                                                              >  1:4693066/1‑150 (MQ=255)
                                                                                         tACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCaaa                                                              <  2:2799449/150‑1 (MQ=255)
                                                                                           cGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAata                                                            >  2:1660139/1‑150 (MQ=255)
                                                                                            gCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatat                                                           <  1:2673257/150‑1 (MQ=255)
                                                                                            gCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAatat                                                           <  2:3775881/150‑1 (MQ=255)
                                                                                                ttCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTc                                                       <  2:4365408/150‑1 (MQ=255)
                                                                                                 tCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcc                                                      >  1:2956647/1‑150 (MQ=255)
                                                                                                 tCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcc                                                      <  2:2847600/150‑1 (MQ=255)
                                                                                                  cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                     <  1:2451916/150‑1 (MQ=255)
                                                                                                  cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                     <  1:3396573/150‑1 (MQ=255)
                                                                                                  cAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTcca                                                     >  2:4205010/1‑150 (MQ=255)
                                                                                                   aGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccag                                                    <  1:1798770/150‑1 (MQ=255)
                                                                                                      aGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTccagcca                                                 >  2:4095372/1‑150 (MQ=255)
                                                                                                         tGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGtt                                              >  2:3997049/1‑150 (MQ=255)
                                                                                                         tGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGtt                                              >  2:4606093/1‑150 (MQ=255)
                                                                                                         tGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCACGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAAAATATTCCAGCCAGtt                                              >  1:132654/1‑150 (MQ=255)
                                                                                                                 tATTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGt                                      >  1:2911628/1‑150 (MQ=255)
                                                                                                                  aTTACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTa                                     >  1:2565336/1‑150 (MQ=255)
                                                                                                                    tACAACATGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTAcc                                   >  1:290060/1‑150 (MQ=255)
                                                                                                                    tACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTAcc                                   <  2:4439238/150‑1 (MQ=255)
                                                                                                                    tACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTATCGATTGATGCCCCCCTTAAAGTGCTATTAGGTTATTCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTAcc                                   >  1:2555556/1‑150 (MQ=255)
                                                                                                                     acaacaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                                  <  1:65350/150‑1 (MQ=255)
                                                                                                                     acaacaTGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCa                                  <  1:2666530/150‑1 (MQ=255)
                                                                                                                       aacaTGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAAc                                >  1:1658627/1‑150 (MQ=255)
                                                                                                                        acaTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACg                               >  1:1429900/1‑150 (MQ=255)
                                                                                                                         caTGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGGTTATGTCGTACCAACGc                              >  1:568830/1‑150 (MQ=255)
                                                                                                                          aTGGGTAATACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCt                             <  2:4267312/150‑1 (MQ=255)
                                                                                                                          aTGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCt                             <  2:4541941/150‑1 (MQ=255)
                                                                                                                             ggTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCt                          <  1:3295713/150‑1 (MQ=255)
                                                                                                                                aaTACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGt                       <  2:1881179/150‑1 (MQ=255)
                                                                                                                                 aTACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATTCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTAACAACGCTTCTGGTa                      >  1:2441865/1‑150 (MQ=255)
                                                                                                                                 aTACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTa                      >  2:3001391/1‑150 (MQ=255)
                                                                                                                                  tACTTTAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTAc                     <  2:1587157/150‑1 (MQ=255)
                                                                                                                                      ttAATGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGccc                 >  2:2116698/1‑150 (MQ=255)
                                                                                                                                        aaTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGt               >  2:4063302/1‑150 (MQ=255)
                                                                                                                                         aTGGTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGtt              >  2:1903599/1‑150 (MQ=255)
                                                                                                                                          tGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa             <  2:827205/150‑1 (MQ=255)
                                                                                                                                          tGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa             <  2:4688147/150‑1 (MQ=255)
                                                                                                                                          tGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTa             <  2:3549982/150‑1 (MQ=255)
                                                                                                                                           ggTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTaa            >  1:1910321/1‑150 (MQ=255)
                                                                                                                                            gTGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAAt           <  2:143281/150‑1 (MQ=255)
                                                                                                                                            gTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAAt           <  2:132032/150‑1 (MQ=255)
                                                                                                                                             tGATTTACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATg          <  1:3508979/150‑1 (MQ=255)
                                                                                                                                               aTTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGc        >  2:4244633/1‑150 (MQ=255)
                                                                                                                                                tttACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCt       <  2:315901/150‑1 (MQ=255)
                                                                                                                                                  tACTCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAt     <  1:4399775/150‑1 (MQ=255)
                                                                                                                                                     gCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTAtttt  <  1:1074549/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACATGGGTAATACTTTAATGGTGATTTACGCCATTGCGAATACCCTGGGACAAGTAGCGGCGCTGGTATTCTCGATTGATGCCCCGCTTAAAGTGCTATTAGGTGATGCTGACAGCAAATATATTCCAGCCAGTTTATGTCGTACCAACGCTTCTGGTACGCCCGTTAATGGCTATTTT  >  CP009273/943424‑943722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑