Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 968,415 G→T 100% S148* (TCG→TAG)  ycbC ← DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273968,4150GT95.5% 1099.1 / 31.6 319S148* (TCG→TAG) ycbCDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base T (157/147);  total (165/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 319 total aligned reads displayed.

AAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTA  >  CP009273/968268‑968561
                                                                                                                                                   |                                                                                                                                                  
aaaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAt                                                                                                                                                  >  1:1113945/1‑150 (MQ=255)
aaaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAt                                                                                                                                                  >  1:1790291/1‑150 (MQ=255)
 aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                 >  2:2657083/1‑150 (MQ=255)
   aaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgc                                                                                                                                               <  2:2130698/150‑1 (MQ=255)
   aaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgc                                                                                                                                               >  2:3243172/1‑150 (MQ=255)
    aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcg                                                                                                                                              >  1:3632892/1‑150 (MQ=255)
       tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCaa                                                                                                                                           >  2:1593290/1‑150 (MQ=255)
       tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCaa                                                                                                                                           >  2:2385339/1‑150 (MQ=255)
         atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAct                                                                                                                                         <  1:1009213/150‑1 (MQ=255)
         atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAct                                                                                                                                         >  2:4196631/1‑150 (MQ=255)
          tcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAActc                                                                                                                                        >  2:4047982/1‑150 (MQ=255)
            gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTg                                                                                                                                      <  2:3639756/150‑1 (MQ=255)
              ccGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGc                                                                                                                                    <  2:1648797/150‑1 (MQ=255)
               cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCa                                                                                                                                   <  1:510614/150‑1 (MQ=255)
                       aTGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTc                                                                                                                           <  1:1568179/150‑1 (MQ=255)
                        tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTcc                                                                                                                          <  1:2289597/150‑1 (MQ=255)
                           gATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCt                                                                                                                       >  1:288994/1‑150 (MQ=255)
                              gCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTa                                                                                                                    <  1:2175131/150‑1 (MQ=255)
                                 gAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTc                                                                                                                 >  1:1902279/1‑150 (MQ=255)
                                   ggTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCAc                                                                                                               >  1:3018947/1‑150 (MQ=255)
                                    gTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCAcc                                                                                                              <  1:4350417/150‑1 (MQ=255)
                                     tCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCg                                                                                                             <  2:2719128/150‑1 (MQ=255)
                                         cagcagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAtt                                                                                                         >  1:2286837/1‑150 (MQ=255)
                                          agcagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTg                                                                                                        >  1:3277770/1‑150 (MQ=255)
                                          agcagGAAAGGGGCGTCACCAAGCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTg                                                                                                        <  2:2459286/150‑1 (MQ=255)
                                            cagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGt                                                                                                      <  2:4484295/150‑1 (MQ=255)
                                            cagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGt                                                                                                      <  2:3034799/150‑1 (MQ=255)
                                               gAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                   <  1:2846240/150‑1 (MQ=255)
                                                aaaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGTGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGttttt                                                                                                  >  2:611380/1‑150 (MQ=255)
                                                 aaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTg                                                                                                 >  1:2685344/1‑150 (MQ=255)
                                                 aaGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCGTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTg                                                                                                 >  1:1487162/1‑150 (MQ=255)
                                                   ggggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCt                                                                                               <  1:2993906/150‑1 (MQ=255)
                                                   ggggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCt                                                                                               <  1:4613571/150‑1 (MQ=255)
                                                   ggggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCt                                                                                               <  2:219552/150‑1 (MQ=255)
                                                     ggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTAc                                                                                             <  2:44469/150‑1 (MQ=255)
                                                            ccAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTccc                                                                                      <  2:3323009/150‑1 (MQ=255)
                                                            ccAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTccc                                                                                      <  1:3968864/150‑1 (MQ=255)
                                                            ccAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTccc                                                                                      <  1:621213/150‑1 (MQ=255)
                                                              aaTCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGt                                                                                    >  1:2242538/1‑150 (MQ=255)
                                                                tCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGa                                                                                  <  2:3196583/150‑1 (MQ=255)
                                                                    cTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGAt                                                                              <  1:3800694/150‑1 (MQ=255)
                                                                    cTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGAt                                                                              >  2:3355208/1‑150 (MQ=255)
                                                                     tGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCTCGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATc                                                                             <  2:1046868/150‑1 (MQ=255)
                                                                     tGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATc                                                                             >  2:2569617/1‑150 (MQ=255)
                                                                      gTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTTGTTTTTGCTACTCCTCCCGTGAAGATCa                                                                            <  2:2165126/150‑1 (MQ=255)
                                                                       tttCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAg                                                                           >  1:4039325/1‑150 (MQ=255)
                                                                         tCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtt                                                                         <  1:2408283/150‑1 (MQ=255)
                                                                         tCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtt                                                                         >  2:197135/1‑150 (MQ=255)
                                                                           aCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtttt                                                                       <  2:1894561/150‑1 (MQ=255)
                                                                            ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTg                                                                      >  2:1306767/1‑150 (MQ=255)
                                                                            ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTg                                                                      >  1:832288/1‑150 (MQ=255)
                                                                             tgctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGa                                                                     <  1:4121873/150‑1 (MQ=255)
                                                                              gctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGAt                                                                    <  1:4297669/150‑1 (MQ=255)
                                                                                    gCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGa                                                              <  2:3117647/150‑1 (MQ=255)
                                                                                        cttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtttt                                                          <  2:3494100/150‑1 (MQ=255)
                                                                                        cttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtttt                                                          >  2:781465/1‑150 (MQ=255)
                                                                                             tcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCgcgc                                                     >  1:1304215/1‑150 (MQ=255)
                                                                                              cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGcc                                                    >  2:3228609/1‑150 (MQ=255)
                                                                                              cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGcc                                                    <  1:4047982/150‑1 (MQ=255)
                                                                                              cGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGcc                                                    >  1:152214/1‑150 (MQ=255)
                                                                                                   tCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAg                                               >  2:4085709/1‑150 (MQ=255)
                                                                                                    cTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGc                                              >  2:222147/1‑150 (MQ=255)
                                                                                                    cTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGc                                              >  1:1747524/1‑150 (MQ=255)
                                                                                                     ttttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCg                                             <  1:3519287/150‑1 (MQ=255)
                                                                                                      tttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGa                                            <  2:2731530/150‑1 (MQ=255)
                                                                                                       ttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGaa                                           >  2:2870218/1‑150 (MQ=255)
                                                                                                        tGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAAt                                          >  1:1982783/1‑150 (MQ=255)
                                                                                                        tGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAAt                                          >  1:2551513/1‑150 (MQ=255)
                                                                                                           cAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGACTAcc                                       >  2:4452188/1‑150 (MQ=255)
                                                                                                           cAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATAcc                                       >  2:3710817/1‑150 (MQ=255)
                                                                                                            aaaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCt                                      >  1:1103043/1‑150 (MQ=255)
                                                                                                            aaaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCt                                      >  1:133029/1‑150 (MQ=255)
                                                                                                            aaaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCt                                      >  2:1023069/1‑150 (MQ=255)
                                                                                                             aaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtt                                     >  1:1192272/1‑150 (MQ=255)
                                                                                                             aaTCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCtt                                     >  2:4262604/1‑150 (MQ=255)
                                                                                                               tCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttca                                   >  2:3014311/1‑150 (MQ=255)
                                                                                                               tCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttca                                   >  2:3636848/1‑150 (MQ=255)
                                                                                                                 cAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcatt                                 <  2:2812414/150‑1 (MQ=255)
                                                                                                                  aGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattc                                >  1:2472542/1‑150 (MQ=255)
                                                                                                                   gggTGATAATTTGCTCGCGTGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                               <  1:611380/150‑1 (MQ=255)
                                                                                                                   gggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                               >  2:3906614/1‑150 (MQ=255)
                                                                                                                    ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAg                              <  1:2088461/150‑1 (MQ=255)
                                                                                                                     gTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAgg                             <  2:1359012/150‑1 (MQ=255)
                                                                                                                        aTAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGt                          >  2:3661981/1‑150 (MQ=255)
                                                                                                                           aTTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGc                       >  1:3701794/1‑150 (MQ=255)
                                                                                                                             ttGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAg                     >  1:2692992/1‑150 (MQ=255)
                                                                                                                             ttGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAg                     >  1:2128821/1‑150 (MQ=255)
                                                                                                                             ttGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAg                     >  1:3386433/1‑150 (MQ=255)
                                                                                                                              tGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGa                    >  2:2259850/1‑150 (MQ=255)
                                                                                                                               gCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGAc                   <  1:2657083/150‑1 (MQ=255)
                                                                                                                                cTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACt                  >  1:575695/1‑150 (MQ=255)
                                                                                                                                  cgcgcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCGGTCTGt                <  1:3203551/150‑1 (MQ=255)
                                                                                                                                   gcgcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGtt               <  2:378555/150‑1 (MQ=255)
                                                                                                                                       ggCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTg           >  2:2218023/1‑150 (MQ=255)
                                                                                                                                       ggCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTg           >  2:1149685/1‑150 (MQ=255)
                                                                                                                                         cACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGAt         <  2:2901780/150‑1 (MQ=255)
                                                                                                                                         cACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGAt         <  2:1852846/150‑1 (MQ=255)
                                                                                                                                            gCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaa      <  1:4480217/150‑1 (MQ=255)
                                                                                                                                                aGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa  <  1:3432913/150‑1 (MQ=255)
                                                                                                                                                aGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa  <  1:3616732/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTA  >  CP009273/968268‑968561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑