Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,224,292 G→A 92.6% D8N (GAT→AAT)  ycgN → UPF0153 family cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,224,2920GA92.6% 714.1 / 34.0 217D8N (GAT→AAT) ycgNUPF0153 family cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (9/7);  new base A (102/98);  total (111/106)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  >  CP009273/1224145‑1224441
                                                                                                                                                   |                                                                                                                                                     
   tGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTa                                                                                                                                                  >  2:3614984/1‑150 (MQ=255)
    gACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAc                                                                                                                                                 <  2:2297364/150‑1 (MQ=255)
      cAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCt                                                                                                                                               <  1:778140/150‑1 (MQ=255)
           aCTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTg                                                                                                                                          >  1:3228581/1‑150 (MQ=255)
                cGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCaaa                                                                                                                                     >  1:3147447/1‑150 (MQ=255)
                 gATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                                    >  1:913060/1‑150 (MQ=255)
                 gATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAg                                                                                                                                    <  1:3068686/150‑1 (MQ=255)
                  aTGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACATTTCTGGCAAAGt                                                                                                                                   >  1:4243455/1‑150 (MQ=255)
                   tGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTa                                                                                                                                  <  1:961972/150‑1 (MQ=255)
                    gggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaa                                                                                                                                 <  1:3261357/150‑1 (MQ=255)
                     ggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                                <  2:2527924/150‑1 (MQ=255)
                     ggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                                <  2:153359/150‑1 (MQ=255)
                      gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaaa                                                                                                                               >  1:3131030/1‑150 (MQ=255)
                      gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaaa                                                                                                                               >  2:1099858/1‑150 (MQ=255)
                       cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                              <  2:1418400/150‑1 (MQ=255)
                       cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAc                                                                                                                              >  2:1090811/1‑150 (MQ=255)
                        aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                             >  2:798811/1‑150 (MQ=255)
                         ttCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGGTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAccc                                                                                                                            >  1:3997256/1‑150 (MQ=255)
                             ttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCTGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTCGa                                                                                                                        >  2:53643/1‑150 (MQ=255)
                             ttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGa                                                                                                                        <  1:1930129/150‑1 (MQ=255)
                             ttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGa                                                                                                                        >  1:3645407/1‑150 (MQ=255)
                              tGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                       >  1:815851/1‑150 (MQ=255)
                              tGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                       <  1:2779420/150‑1 (MQ=255)
                               gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACg                                                                                                                      >  2:2829648/1‑150 (MQ=255)
                               gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACg                                                                                                                      <  2:404549/150‑1 (MQ=255)
                                 cAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCTGCAAAACTTGCAACGCTGTGCCAGACTGTTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGAAGAGCAAAGAACCTTTCTGGCAAAGTAAAACCCTGGACGaa                                                                                                                    >  1:3513448/1‑150 (MQ=255)
                                 cAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaa                                                                                                                    >  1:4474779/1‑150 (MQ=255)
                                  aaCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaaa                                                                                                                   <  1:3384489/150‑1 (MQ=255)
                                   aCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAAt                                                                                                                  <  2:1399378/150‑1 (MQ=255)
                                    cTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCCAAGTAAAACCCTGGACGAAATg                                                                                                                 >  2:42636/1‑150 (MQ=255)
                                        cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                             >  2:4085420/1‑150 (MQ=255)
                                          ttttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGAt                                                                                                           <  1:3032703/150‑1 (MQ=255)
                                          tttaGTAATACTTTTTGCCGACTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTTCGATTTAACGTAATGGAGGAAAACATGATGAGCAATGTACCTTTATGTCAAAGTAAAACCCTGGACGAAATGAGCGAt                                                                                                           >  2:544885/1‑150 (MQ=255)
                                             tGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCg                                                                                                        <  1:2901034/150‑1 (MQ=255)
                                             tGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCg                                                                                                        >  1:4043182/1‑150 (MQ=255)
                                              gTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCgg                                                                                                       >  1:2318528/1‑150 (MQ=255)
                                               tAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGa                                                                                                      >  1:810540/1‑150 (MQ=255)
                                                 aTACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAAt                                                                                                    <  1:1297241/150‑1 (MQ=255)
                                                   aCTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATgg                                                                                                  >  1:4375580/1‑150 (MQ=255)
                                                    cTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                                 >  1:3818817/1‑150 (MQ=255)
                                                    cTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATggg                                                                                                 <  2:2430851/150‑1 (MQ=255)
                                                      ttttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAg                                                                                               <  2:4734327/150‑1 (MQ=255)
                                                       tttGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGt                                                                                              <  1:244819/150‑1 (MQ=255)
                                                         tGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCg                                                                                            <  2:3552544/150‑1 (MQ=255)
                                                         tGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCg                                                                                            >  2:3108602/1‑150 (MQ=255)
                                                              ccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtg                                                                                       <  2:2180014/150‑1 (MQ=255)
                                                               cTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtgt                                                                                      <  2:604154/150‑1 (MQ=255)
                                                                tGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTtgtgtg                                                                                     >  1:1438/1‑150 (MQ=255)
                                                                    aGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTgg                                                                                 >  2:1769534/1‑150 (MQ=255)
                                                                    aGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATgg                                                                                 >  1:3933651/1‑150 (MQ=255)
                                                                      cggcggCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGtt                                                                               <  1:2027323/150‑1 (MQ=255)
                                                                       ggcggcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGTTGTGATGGTTg                                                                              >  2:4497690/1‑150 (MQ=255)
                                                                         cggcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCg                                                                            <  1:2546926/150‑1 (MQ=255)
                                                                           gcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGt                                                                          <  2:2027/150‑1 (MQ=255)
                                                                            cAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTc                                                                         <  1:2336776/150‑1 (MQ=255)
                                                                             aaaaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCa                                                                        >  2:2255102/1‑150 (MQ=255)
                                                                                 cTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAAGGGCGGAACACCTGATGAGCAATGGACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGGTGCGGTCAgtgt                                                                    <  1:321497/150‑1 (MQ=255)
                                                                                        cGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGc                                                             >  2:866883/1‑150 (MQ=255)
                                                                                         gCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCa                                                            >  1:1140837/1‑150 (MQ=255)
                                                                                           tgtgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATa                                                          >  2:3789131/1‑150 (MQ=255)
                                                                                                cAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTg                                                     <  1:1811826/150‑1 (MQ=255)
                                                                                                 aGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTga                                                    <  2:3660278/150‑1 (MQ=255)
                                                                                                      ggTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggat                                               <  1:2170771/150‑1 (MQ=255)
                                                                                                       tttATAAGGTGAGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggatg                                              <  1:3307642/149‑1 (MQ=255)
                                                                                                       gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggatg                                              <  1:1978143/150‑1 (MQ=255)
                                                                                                       gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggatg                                              <  1:1978097/150‑1 (MQ=255)
                                                                                                          ataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCGGCAAAAACTGATGGATGaat                                           >  1:949803/1‑149 (MQ=255)
                                                                                                           taAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGa                                          <  1:1613872/150‑1 (MQ=255)
                                                                                                            aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGCGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGac                                         >  1:2740916/1‑150 (MQ=255)
                                                                                                            aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGac                                         >  2:863614/1‑150 (MQ=255)
                                                                                                            aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaa                                         >  1:2526008/1‑149 (MQ=255)
                                                                                                             aGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaca                                        >  2:165641/1‑150 (MQ=255)
                                                                                                             aGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaca                                        <  1:1384357/150‑1 (MQ=255)
                                                                                                             aGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGCGGTCAGTGTTGCCTGCCTAAACTGATGGATGAAGaca                                        >  2:3396858/1‑150 (MQ=255)
                                                                                                              ggTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGacac                                       >  2:1351859/1‑150 (MQ=255)
                                                                                                               gTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACAcc                                      <  2:2066008/150‑1 (MQ=255)
                                                                                                                tGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTTCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACAccc                                     >  2:1190819/1‑149 (MQ=255)
                                                                                                                  cGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcgac                                   <  2:913060/150‑1 (MQ=255)
                                                                                                                     tttAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGaa                                >  2:4160457/1‑150 (MQ=255)
                                                                                                                       tAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGGTGCCAGCAAAAACTGATGGATGAAGAAACCGACGAAAt                              >  2:1607227/1‑150 (MQ=255)
                                                                                                                        aaCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATc                             >  2:3063779/1‑150 (MQ=255)
                                                                                                                         aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTTATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATTAAGACACCGACGAAATCt                            >  2:2807351/1‑150 (MQ=255)
                                                                                                                         aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCt                            <  1:3615536/150‑1 (MQ=255)
                                                                                                                         aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCt                            <  1:209948/150‑1 (MQ=255)
                                                                                                                         aCGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCt                            <  2:3645505/150‑1 (MQ=255)
                                                                                                                           gTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTAc                          >  1:662987/1‑150 (MQ=255)
                                                                                                                           gTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTATTGCCTGCATAAACTGATGGATGAAGACACCGACGAAAACTAc                          >  2:2041392/1‑150 (MQ=255)
                                                                                                                             aaTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGTTGGTTTCGGTCAGTGTTGCCTGCATAAAATGATGGATGAAGACACCGACGAAAACTaaat                        >  1:2818102/1‑147 (MQ=255)
                                                                                                                               tGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCa                      >  2:4384277/1‑150 (MQ=255)
                                                                                                                                 gCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACt                    <  2:29786/150‑1 (MQ=255)
                                                                                                                                   ggAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAACTCTACTTCACTaa                  >  2:4597070/1‑150 (MQ=255)
                                                                                                                                    gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTGGTGTGATGGTTGCGGTCCGTGTTGCCTGCATAAACTGATGGATGAAGAAACCGACGAAATCTACTTCAATAAc                 >  2:848627/1‑150 (MQ=255)
                                                                                                                                    gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAAc                 >  2:3133431/1‑150 (MQ=255)
                                                                                                                                    gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGTTTGTTGCGGTCAGTGTTTCCTGCATAAAAAGATGGATGAAGACAACCACCAAATCAACTTCACAAAc                 >  1:2775395/1‑150 (MQ=255)
                                                                                                                                          cTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTTCCTGCATAAAATGATGGATTAATACACCGACGAAATCTAATTCACTAACGTCGcc           >  1:1524116/1‑150 (MQ=255)
                                                                                                                                           tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCt          <  1:1400665/150‑1 (MQ=255)
                                                                                                                                           tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCt          <  1:535760/150‑1 (MQ=255)
                                                                                                                                               gaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGGTGGTTGGGGTCAGTGTTTGCTGCATAAAATTATGGATGAATACACCGACGAAATATAAATAACTAACCTCGCCAGtag      >  1:4393879/1‑148 (MQ=255)
                                                                                                                                                  cGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGACTGGGAGTCGTTGTGTGATGGTTGCGGTCAGGGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCa   >  2:3635402/1‑150 (MQ=255)
                                                                                                                                                   gATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATATACTTCACTAACGTCGCCTGTCGCCAg  >  1:3795129/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAG  >  CP009273/1224145‑1224441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑