BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,472,138	G→T	8.6%	A87A (GCG→GCT)	ynbA →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,472,138	0	G	T	8.6%	646.4 / 31.7	175	A87A (GCG→GCT)	ynbA	inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (77/83); new base T (6/9); total (83/92)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 177 total aligned reads displayed.

TTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTAA  >  CP009273/1471990‑1472287

tttCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGa                                                                                                                                                      >  1:921182/1‑150 (MQ=255)

 ttCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAt                                                                                                                                                     <  1:852363/150‑1 (MQ=255)

  tctcTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAtt                                                                                                                                                    <  2:3201360/150‑1 (MQ=255)

  tctcTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAtt                                                                                                                                                    <  1:3709923/150‑1 (MQ=255)

   ctctTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAttt                                                                                                                                                   <  1:735645/150‑1 (MQ=255)

    tcttctTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAtttt                                                                                                                                                  <  2:4277579/150‑1 (MQ=255)

    tcttctTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGAtttt                                                                                                                                                  >  2:2512937/1‑150 (MQ=255)

     cttcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTg                                                                                                                                                 >  1:2910996/1‑150 (MQ=255)

     cttcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTg                                                                                                                                                 >  2:4000419/1‑150 (MQ=255)

     cttcttACCGGATCGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTg                                                                                                                                                 >  1:2911140/1‑150 (MQ=255)

      ttcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGa                                                                                                                                                >  2:3591663/1‑150 (MQ=255)

      ttcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGa                                                                                                                                                <  1:626735/150‑1 (MQ=255)

       tcttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGaa                                                                                                                                               <  2:3462177/150‑1 (MQ=255)

         ttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATg                                                                                                                                             <  1:2528236/150‑1 (MQ=255)

         ttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATg                                                                                                                                             <  1:3113748/150‑1 (MQ=255)

         ttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATg                                                                                                                                             <  2:4124095/150‑1 (MQ=255)

         ttACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATg                                                                                                                                             >  1:1008064/1‑150 (MQ=255)

         ttACCGGAGTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATg                                                                                                                                             <  1:1021909/150‑1 (MQ=255)

              ggATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACt                                                                                                                                        <  1:398964/150‑1 (MQ=255)

                 ttGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGc                                                                                                                                     <  1:4756426/150‑1 (MQ=255)

                    cTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGAt                                                                                                                                  <  1:1515675/150‑1 (MQ=255)

                    cTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGAt                                                                                                                                  <  2:3332852/150‑1 (MQ=255)

                          aTGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTAtt                                                                                                                            >  1:1967015/1‑150 (MQ=255)

                             ttGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTcc                                                                                                                         <  2:229990/150‑1 (MQ=255)

                              tGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCg                                                                                                                        >  2:1611216/1‑150 (MQ=255)

                               ggcggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGa                                                                                                                       >  2:919320/1‑150 (MQ=255)

                                gcggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGat                                                                                                                      <  1:1545557/150‑1 (MQ=255)

                                gcggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGat                                                                                                                      <  2:1722218/150‑1 (MQ=255)

                                gcggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGat                                                                                                                      <  1:1545514/150‑1 (MQ=255)

                                 cggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGata                                                                                                                     >  1:4613203/1‑150 (MQ=255)

                                  ggcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGatat                                                                                                                    <  1:1455244/150‑1 (MQ=255)

                                   gcACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATAtt                                                                                                                   <  2:555789/150‑1 (MQ=255)

                                    cacaACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                  >  1:1840012/1‑150 (MQ=255)

                                    cacaACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTg                                                                                                                  <  1:2949593/150‑1 (MQ=255)

                                     acaACCCATCCTCTTTTTGCTAGTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTgc                                                                                                                 <  1:2395652/150‑1 (MQ=255)

                                      caACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTgcg                                                                                                                >  1:2492355/1‑150 (MQ=255)

                                      caACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTgcg                                                                                                                >  1:4695445/1‑150 (MQ=255)

                                        aCCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGct                                                                                                              >  2:2783385/1‑150 (MQ=255)

                                          ccATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGctct                                                                                                            >  1:4729207/1‑150 (MQ=255)

                                            aTCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATTTTATTTCCGATATTGCGctctct                                                                                                          >  1:1769601/1‑148 (MQ=255)

                                            aTCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGctctct                                                                                                          >  1:1769570/1‑148 (MQ=255)

                                              ccTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTAttt                                                                                                        <  2:4197116/150‑1 (MQ=255)

                                               ctctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTCTTTa                                                                                                       >  2:1036969/1‑150 (MQ=255)

                                               ctctTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTa                                                                                                       <  1:3341000/150‑1 (MQ=255)

                                                    tttGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatt                                                                                                  >  1:2277330/1‑150 (MQ=255)

                                                     ttGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattt                                                                                                 <  2:203314/150‑1 (MQ=255)

                                                        cTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCattttta                                                                                              >  1:1895862/1‑150 (MQ=255)

                                                         tATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttat                                                                                             >  2:333285/1‑150 (MQ=255)

                                                         tATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttat                                                                                             <  2:4729452/150‑1 (MQ=255)

                                                          aTTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttatt                                                                                            <  2:1918332/150‑1 (MQ=255)

                                                          aTTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttatt                                                                                            <  1:27946/150‑1 (MQ=255)

                                                          aTTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttatt                                                                                            <  2:4302384/150‑1 (MQ=255)

                                                           ttGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttattt                                                                                           <  2:1646083/150‑1 (MQ=255)

                                                           ttGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttattt                                                                                           <  2:1643715/150‑1 (MQ=255)

                                                           ttGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCatttttattt                                                                                           <  1:3579949/150‑1 (MQ=255)

                                                                 aTCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCg                                                                                     >  1:1306587/1‑150 (MQ=255)

                                                                 aTCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCg                                                                                     <  2:2313952/150‑1 (MQ=255)

                                                                    gTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGaa                                                                                  >  2:3795134/1‑150 (MQ=255)

                                                                    gTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGaa                                                                                  >  2:3797958/1‑150 (MQ=255)

                                                                    gTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGaa                                                                                  >  1:2730032/1‑150 (MQ=255)

                                                                       cTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGt                                                                               >  1:2788410/1‑150 (MQ=255)

                                                                          tttATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAAc                                                                            >  1:1556750/1‑150 (MQ=255)

                                                                              tCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCAt                                                                        >  2:3379730/1‑150 (MQ=255)

                                                                               ccGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATc                                                                       <  1:3445981/150‑1 (MQ=255)

                                                                                 ttATGTCGCTCAATGCGCTGGATGGCTTGTTGGCTCGTGAGTGCAACCAGCAAACTCGTATGTGGGCGATTTTGATTGAGACTTGCGATGTTATTTCAGATATTGCTCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCAc                                                                     <  2:4016981/149‑1 (MQ=255)

                                                                                     ggCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGt                                                                 <  1:1987576/150‑1 (MQ=255)

                                                                                      gcgcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTg                                                                <  2:3530302/150‑1 (MQ=255)

                                                                                      gcgcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTg                                                                <  2:1204969/150‑1 (MQ=255)

                                                                                      gcgcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTg                                                                >  1:2493815/1‑150 (MQ=255)

                                                                                        gcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                              <  2:4136745/150‑1 (MQ=255)

                                                                                        gcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                              >  2:1752399/1‑150 (MQ=255)

                                                                                        gcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                              >  2:2580079/1‑150 (MQ=255)

                                                                                        gcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                              >  2:1752497/1‑150 (MQ=255)

                                                                                        gcTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                              <  2:3013694/150‑1 (MQ=255)

                                                                                        gcTCAATGCGCTGGATGGCAGGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGAt                                                              <  2:4580375/150‑1 (MQ=255)

                                                                                         cTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATa                                                             <  2:3483835/150‑1 (MQ=255)

                                                                                          tCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATAc                                                            <  1:411368/150‑1 (MQ=255)

                                                                                            aaTGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATGTTTACCGGAAAGTAACGCATCACTCGTGATACTc                                                          >  2:3680916/1‑150 (MQ=255)

                                                                                             aggcgcCGGACGGCATGTTGGCGCGTGACTGCAACCAGCAAACACGTCTGGGGGCGATTTGGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCa                                                         <  1:1714933/148‑1 (MQ=255)

                                                                                                 gcTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCTATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGtt                                                     <  1:1194172/150‑1 (MQ=255)

                                                                                                   tGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTAt                                                   <  1:2068059/150‑1 (MQ=255)

                                                                                                    ggATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTAtt                                                  >  1:4283204/1‑150 (MQ=255)

                                                                                                    ggATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTAtt                                                  <  2:3352482/150‑1 (MQ=255)

                                                                                                       tGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTg                                               >  1:2862390/1‑150 (MQ=255)

                                                                                                          cATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCAc                                            >  1:4037394/1‑150 (MQ=255)

                                                                                                           aTGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCAcc                                           <  2:4284503/150‑1 (MQ=255)

                                                                                                           aTGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCAcc                                           <  2:4284406/150‑1 (MQ=255)

                                                                                                            tGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCa                                          <  2:1840012/150‑1 (MQ=255)

                                                                                                                      tGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGa                                >  2:1417511/1‑150 (MQ=255)

                                                                                                                        aGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGt                              >  2:2817040/1‑150 (MQ=255)

                                                                                                                          tGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATATCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTAATGTTATTTTGCACCATATTGACCGAGttt                            >  2:3896974/1‑150 (MQ=255)

                                                                                                                           gCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGtttt                           <  1:159487/150‑1 (MQ=255)

                                                                                                                            cAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTg                          <  1:4099686/150‑1 (MQ=255)

                                                                                                                            cAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTg                          <  2:493182/150‑1 (MQ=255)

                                                                                                                                  gCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGttt                    <  2:702451/150‑1 (MQ=255)

                                                                                                                                    aaaCACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTAc                  >  1:3917324/1‑150 (MQ=255)

                                                                                                                                    aaaCACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGGTTTTCGGTTTAc                  >  1:2383401/1‑150 (MQ=255)

                                                                                                                                         cGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTcgcg             <  1:1256460/150‑1 (MQ=255)

                                                                                                                                            cTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTGTACTCGCGCAg          >  2:2772453/1‑150 (MQ=255)

                                                                                                                                                    gATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTaa  >  1:1341903/1‑150 (MQ=255)

TTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTATTGCCCATCGTGCTTTTTATCCGTATGGCGCTCAATGCGCTGGATGGCATGTTGGCGCGTGAGTGCAACCAGCAAACACGTCTGGGGGCGATTTTGAATGAGACTGGCGATGTTATTTCCGATATTGCGCTCTATTTACCATTTTTATTTTTACCGGAAAGTAACGCATCACTCGTGATACTCATGTTATTTTGCACCATATTGACCGAGTTTTGCGGTTTACTCGCGCAGACGATTAA  >  CP009273/1471990‑1472287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`