Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,501,734 G→T 13.4% D233Y (GAT→TAT)  ydcP → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,501,7340GT13.4% 553.1 / 52.5 170D233Y (GAT→TAT) ydcPputative peptidase
Reads supporting (aligned to +/- strand):  ref base G (70/77);  new base T (12/11);  total (82/88)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CAGGGCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACTGAACA  >  CP009273/1501585‑1501882
                                                                                                                                                     |                                                                                                                                                    
cAGGGCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgattg                                                                                                                                                      <  2:2870264/150‑1 (MQ=255)
cAGGGCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgattg                                                                                                                                                      <  2:2869754/150‑1 (MQ=255)
 aGGGCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgattga                                                                                                                                                     <  2:4325157/150‑1 (MQ=255)
  gggCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgatttat                                                                                                                                                    <  1:3818826/150‑1 (MQ=255)
  gggCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgattgat                                                                                                                                                    >  2:1811929/1‑150 (MQ=255)
  gggCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgattgat                                                                                                                                                    <  1:3762518/150‑1 (MQ=255)
  gggCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCtgattgat                                                                                                                                                    <  1:1965764/150‑1 (MQ=255)
   ggCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATg                                                                                                                                                   <  2:2363740/150‑1 (MQ=255)
        aGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGgt                                                                                                                                              >  2:4020693/1‑150 (MQ=255)
             cAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACg                                                                                                                                         >  2:2335201/1‑150 (MQ=255)
             cAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACAAATTGAAAGAAGATCAGGGTCGGGTGGTTTCCTATGAAAAAAATCTGCTGTCGATGAAAGATAAAGAAAAGACTTCCCACCTCGGCGCGCTGATTTATGCTGGTGTACg                                                                                                                                         >  1:836018/1‑150 (MQ=255)
                 cGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTcc                                                                                                                                     >  1:4285503/1‑150 (MQ=255)
                  gTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCt                                                                                                                                    <  2:4133675/150‑1 (MQ=255)
                   tGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCtt                                                                                                                                   <  1:4288202/150‑1 (MQ=255)
                        aTTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGa                                                                                                                              >  1:2684332/1‑150 (MQ=255)
                          tGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGAtt                                                                                                                            <  2:1007578/150‑1 (MQ=255)
                          tGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGAtt                                                                                                                            >  2:1594123/1‑150 (MQ=255)
                          tGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGAtt                                                                                                                            <  1:2778540/150‑1 (MQ=255)
                           gCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTg                                                                                                                           <  1:1896620/150‑1 (MQ=255)
                              cGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAg                                                                                                                        >  1:2107374/1‑150 (MQ=255)
                                  ggCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAAAGATCAGACTGCCAAACTCGGCGCGCTGATTGATGCAGGTGTACTCTCCTTCAAGATTGAAGGGCg                                                                                                                    >  2:259200/1‑150 (MQ=255)
                                    cGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGtt                                                                                                                  <  2:3962282/150‑1 (MQ=255)
                                     gTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTTGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTa                                                                                                                 <  1:3678570/150‑1 (MQ=255)
                                      tGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTAc                                                                                                                >  1:4154340/1‑150 (MQ=255)
                                         cGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACaaa                                                                                                             >  1:3577306/1‑150 (MQ=255)
                                              gCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGatat                                                                                                        <  1:2398327/150‑1 (MQ=255)
                                                cATACACATTGAAAGACGATCATGGGCGGGTGGTTTCCTATGAAAAACATCTTCTGTCGATGAAAGATAACGATCAGACTGCAAACCTCGGCGCGCTGATTGATTCTGGTTTACTCTCCTTCAAGATTGAAGTGCGTTACAAAGATATGa                                                                                                      >  1:3786709/1‑150 (MQ=255)
                                                cATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGa                                                                                                      >  1:2343773/1‑150 (MQ=255)
                                                cATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGa                                                                                                      >  1:3610660/1‑150 (MQ=255)
                                                    cacaTTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTa                                                                                                  <  1:4525137/150‑1 (MQ=255)
                                                    cacaTTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTa                                                                                                  >  2:1741399/1‑150 (MQ=255)
                                                         tGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTga                                                                                             <  1:3140572/150‑1 (MQ=255)
                                                                 gATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATc                                                                                     >  1:831851/1‑150 (MQ=255)
                                                                 gATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATc                                                                                     >  1:806438/1‑150 (MQ=255)
                                                                     aGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCg                                                                                 >  1:3109410/1‑150 (MQ=255)
                                                                           gggTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCAtt                                                                           <  1:374753/150‑1 (MQ=255)
                                                                           gggTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCAtt                                                                           <  1:4584604/150‑1 (MQ=255)
                                                                           gggTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCAtt                                                                           <  1:285778/150‑1 (MQ=255)
                                                                            ggtggtTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTa                                                                          >  2:2024915/1‑150 (MQ=255)
                                                                             gtggtTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTAt                                                                         <  1:1697375/150‑1 (MQ=255)
                                                                              tggtTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATc                                                                        <  2:924750/150‑1 (MQ=255)
                                                                              tggtTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATc                                                                        >  2:524832/1‑150 (MQ=255)
                                                                                   tCCTATGAAAAACATCTTCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGATGGGCTTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAg                                                                   <  1:2065559/150‑1 (MQ=255)
                                                                                   tCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAg                                                                   <  1:4228378/150‑1 (MQ=255)
                                                                                    ccTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGa                                                                  <  2:2862097/150‑1 (MQ=255)
                                                                                      tATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATg                                                                >  2:1631400/1‑150 (MQ=255)
                                                                                         gAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCtt                                                             <  1:4496940/150‑1 (MQ=255)
                                                                                         gAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCtt                                                             <  1:4359935/150‑1 (MQ=255)
                                                                                         gAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCtt                                                             <  1:1924919/150‑1 (MQ=255)
                                                                                          aaaaaCATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTg                                                            >  1:52567/1‑150 (MQ=255)
                                                                                          aaaaaCATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTg                                                            <  1:317652/150‑1 (MQ=255)
                                                                                          aaaaaCATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTg                                                            >  2:4307782/1‑150 (MQ=255)
                                                                                            aaaCATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGAt                                                          >  1:2193312/1‑150 (MQ=255)
                                                                                             aaCATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATg                                                         <  2:42513/150‑1 (MQ=255)
                                                                                                aTCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCa                                                      >  1:4709925/1‑150 (MQ=255)
                                                                                                  ctgctgTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCatt                                                    <  2:2542035/150‑1 (MQ=255)
                                                                                                  ctgctgTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCatt                                                    <  2:3696332/150‑1 (MQ=255)
                                                                                                  ctgctgTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCatt                                                    >  1:1873909/1‑150 (MQ=255)
                                                                                                   tgctgTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCatta                                                   >  2:2993345/1‑150 (MQ=255)
                                                                                                    gctgTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCattat                                                  <  2:2199352/150‑1 (MQ=255)
                                                                                                      tgTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCCTTATTg                                                >  2:4102920/1‑150 (MQ=255)
                                                                                                        tCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTgaa                                              >  1:1561555/1‑150 (MQ=255)
                                                                                                          gATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTgaaga                                            >  2:855748/1‑150 (MQ=255)
                                                                                                          gATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCAGTATTgacga                                            >  2:855957/1‑150 (MQ=255)
                                                                                                           aTGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTCCGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTgaagaa                                           <  1:2024915/150‑1 (MQ=255)
                                                                                                             gAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACg                                         >  2:223809/1‑150 (MQ=255)
                                                                                                              aaaGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGt                                        >  2:1313681/1‑150 (MQ=255)
                                                                                                                aGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTgg                                      >  1:617990/1‑150 (MQ=255)
                                                                                                                aGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTgg                                      >  2:2408183/1‑150 (MQ=255)
                                                                                                                  aTAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCg                                    >  1:741426/1‑150 (MQ=255)
                                                                                                                  aTAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCg                                    <  1:129564/150‑1 (MQ=255)
                                                                                                                  aTAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCg                                    >  1:3112785/1‑150 (MQ=255)
                                                                                                                  aTAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCg                                    >  2:102536/1‑150 (MQ=255)
                                                                                                                   tAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGa                                   >  2:3419678/1‑150 (MQ=255)
                                                                                                                       gATCAGACTGCCAACCTCGGCGCGCTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTg                               <  2:4708046/150‑1 (MQ=255)
                                                                                                                       gATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTg                               >  2:1212896/1‑150 (MQ=255)
                                                                                                                          cAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGgcg                            <  1:25895/150‑1 (MQ=255)
                                                                                                                              cTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGgcgcgcg                        >  1:173465/1‑150 (MQ=255)
                                                                                                                                gCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTTATGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCt                      <  2:3786709/150‑1 (MQ=255)
                                                                                                                                gCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCt                      <  1:1119678/150‑1 (MQ=255)
                                                                                                                                  cAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtc                    <  1:1465491/150‑1 (MQ=255)
                                                                                                                                  cAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtc                    <  1:1338855/150‑1 (MQ=255)
                                                                                                                                   aaCCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTTAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGACAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtca                   >  1:4076487/1‑150 (MQ=255)
                                                                                                                                   aaCCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtca                   >  1:3756957/1‑150 (MQ=255)
                                                                                                                                    aCCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtcat                  <  2:3590443/150‑1 (MQ=255)
                                                                                                                                    aCCTCGGCGCGCTGAGTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtcat                  <  2:3591583/150‑1 (MQ=255)
                                                                                                                                      cTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtcatca                <  2:2911498/150‑1 (MQ=255)
                                                                                                                                      cTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtcatca                <  1:2665353/150‑1 (MQ=255)
                                                                                                                                      cTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTtcatca                <  2:3194543/150‑1 (MQ=255)
                                                                                                                                        cGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAgg              <  2:4423840/150‑1 (MQ=255)
                                                                                                                                         ggCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGt             >  2:3784503/1‑150 (MQ=255)
                                                                                                                                         ggCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGt             >  1:4465178/1‑150 (MQ=255)
                                                                                                                                          gcgcgcTGATTTATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTc            >  2:2827202/1‑150 (MQ=255)
                                                                                                                                          gcgcgcTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTc            <  2:131565/150‑1 (MQ=255)
                                                                                                                                              gcTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTAc        <  1:3655846/150‑1 (MQ=255)
                                                                                                                                                tgattgatGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACta      <  1:2605969/150‑2 (MQ=255)
                                                                                                                                                tgattgatGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACTg      >  1:3692102/1‑150 (MQ=255)
                                                                                                                                                 gattgatGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACTGa     >  2:279691/1‑150 (MQ=255)
                                                                                                                                                   ttgatGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCGACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCAGTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACTGAAc   <  2:4517695/150‑1 (MQ=255)
                                                                                                                                                    tgatGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACTGAACa  >  2:1087529/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGGGCGTAGCGCCAACCGTGGCGATTGCTCGCAGGCGTGCCGTTTGCCATACACATTGAAAGACGATCAGGGGCGGGTGGTTTCCTATGAAAAACATCTGCTGTCGATGAAAGATAACGATCAGACTGCCAACCTCGGCGCGCTGATTGATGCTGGTGTACGCTCCTTCAAGATTGAAGGGCGTTACAAAGATATGAGCTACGTGAAGAATATCACCGCCCATTATCGCCAGATGCTTGATGCCATTATTGAAGAACGTGGCGATCTGGCGCGCGCTTCATCAGGTCGTACTGAACA  >  CP009273/1501585‑1501882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑