| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 132,632 | A→T | 12.4% | V107E (GTG→GAG) | speE ← | spermidine synthase (putrescine aminopropyltransferase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 132,632 | 0 | A | T | 12.4% | 758.1 / 67.7 | 239 | V107E (GTG→GAG) | speE | spermidine synthase (putrescine aminopropyltransferase) |
| Reads supporting (aligned to +/- strand): ref base A (103/105); new base T (17/13); total (121/118) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 251 total aligned reads displayed.
AGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCG > CP009273/132483‑132780 | gaTAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCCCATCGATTTacaa > 1:1579162/1‑149 (MQ=255) aTAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTccacc > 2:190770/1‑150 (MQ=255) aTGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATc < 1:3045478/150‑1 (MQ=255) tGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCg > 1:2298648/1‑150 (MQ=255) tGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGAT‑TGTTGGGTAGATAATTACGGCAGAACGATAAGACACCCGCAACGATTTacaacaaag > 2:3608360/1‑147 (MQ=255) gACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATAGt > 2:3162323/1‑150 (MQ=255) gACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATAATGACGGCAGAAAGATACGACACCCGCATCGATTTACACCATCGt > 2:1976718/1‑150 (MQ=255) aCATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGAT‑GGTTGGGTAGATACAGACGGCAGAACGATACGACACCCCCAACGATTTCCACCAACGTg > 1:941511/1‑150 (MQ=255) cATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGa > 2:3529036/1‑150 (MQ=255) cATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGa > 2:371671/1‑150 (MQ=255) aTCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGGTTGGGT‑GATACATAAGGCAGAACCAAACCACACCCCCAACGATTTCCACCATAGTGAt > 1:4362776/1‑150 (MQ=255) cAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTACTCCATCGTGATTg > 1:667683/1‑150 (MQ=255) aaaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACATAAGGCACAACGATACGACACCCGCATCGATTTCCACCAACGTGATTGa > 2:438516/1‑150 (MQ=255) aaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTCCCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGAc > 2:3909468/1‑150 (MQ=255) aGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGTCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACt > 2:2961042/1‑150 (MQ=255) ggTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTc < 1:103603/150‑1 (MQ=255) ggTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTc > 2:2185866/1‑150 (MQ=255) gTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCa > 2:3384767/1‑150 (MQ=255) tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACg < 1:2377379/150‑1 (MQ=255) tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACg < 1:2643879/150‑1 (MQ=255) gctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGtt < 1:2540868/150‑1 (MQ=255) gctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGtt < 2:708129/150‑1 (MQ=255) tggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGtttt < 2:2517212/150‑1 (MQ=255) tggTTTGATTAACGAAATTGACGCCATCGACGATCACCAGATTAAAGCGCGGATCGTCGTAGCTACCGGCTTTAT‑TTTTTGGTAGATACTGACGGCATAACGATAAGAAACACGCAAAGAATTCCACCATCGTGATTGACTCCACGttgt > 2:1409353/1‑148 (MQ=255) ggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGttttt > 2:4144249/1‑150 (MQ=255) ggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGttttt > 2:247229/1‑150 (MQ=255) tttGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAAAGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTAt > 1:215073/1‑150 (MQ=255) ttGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATg < 1:52301/150‑1 (MQ=255) tGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGt < 1:3732160/150‑1 (MQ=255) tGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGt < 1:4222644/150‑1 (MQ=255) aTTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCg > 2:2125677/1‑150 (MQ=255) ttAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCgg > 2:305819/1‑150 (MQ=255) aaCGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCgggg > 1:1855464/1‑149 (MQ=255) gAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTAGGGTTAc > 2:528529/1‑150 (MQ=255) aaaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACt > 2:4356927/1‑150 (MQ=255) aaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACtt < 2:2719683/150‑1 (MQ=255) aaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCAACGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTAcgt > 1:2620891/1‑148 (MQ=255) gACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACg < 1:1314642/150‑1 (MQ=255) gACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACg < 1:4681226/150‑1 (MQ=255) cGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACgca < 2:3434329/150‑1 (MQ=255) cATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑TGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCCTCGATTTCCACCAACGTGTTTTACTCAACGTTTTTATGTGGGGTTAATTCACgcagca > 1:1980432/1‑150 (MQ=255) tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg < 2:2445056/150‑1 (MQ=255) tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg < 2:1094197/150‑1 (MQ=255) tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg < 1:3142764/150‑1 (MQ=255) tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACATACGGCAGAACGAAAAGACACCCCCATCGATTTCCACCAACCGGATTGACACAACGTTTTTATGTAGGGTTACTTCACGCAGCATg > 2:1623843/1‑150 (MQ=255) cgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATgg > 2:1803968/1‑150 (MQ=255) tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa < 1:612145/150‑1 (MQ=255) tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa < 1:2959633/150‑1 (MQ=255) tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa < 1:4748443/150‑1 (MQ=255) gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATTTCGGGTTACTTAACGCAGCATGGCAcc > 1:3038979/1‑150 (MQ=255) gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCAcc > 1:1507959/1‑150 (MQ=255) tCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGt > 1:3538792/1‑150 (MQ=255) cACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTc > 2:3753783/1‑150 (MQ=255) cAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgcc < 2:1086704/150‑1 (MQ=255) ttAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccg < 1:683456/150‑1 (MQ=255) ttAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccg < 2:665152/150‑1 (MQ=255) ttAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccg < 2:3737747/150‑1 (MQ=255) tAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccgc > 1:2724117/1‑150 (MQ=255) aaGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGccgccgccg < 2:405599/150‑1 (MQ=255) aaGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGccgccgccg < 1:3437013/150‑1 (MQ=255) aGCGCGGATCGTCGTAGCTACCGGCGTTAA‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGCTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACATCACGCCGCATGGCACCGTCcccgcccccca > 1:3852607/1‑148 (MQ=255) gcgGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATaa < 2:1334833/150‑1 (MQ=255) ggATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATc < 1:71032/150‑1 (MQ=255) cgtAGCTACCGGCGTTAT‑GGTTGGGTAGATACTTAAGGCAGAACGATACCACACCCCCAACGATTTACACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCCTGGCACCGTCGCCGCCCCCGCTAAACAGcccaa > 1:3321343/1‑149 (MQ=255) gtAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGGACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATg < 2:1219890/150‑1 (MQ=255) gtAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATg < 1:266185/150‑1 (MQ=255) gCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGttt < 2:4030568/150‑1 (MQ=255) cTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTc < 1:3210356/150‑1 (MQ=255) ccGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTcgcg < 2:2293786/150‑1 (MQ=255) ccGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTcgcg < 2:3226878/150‑1 (MQ=255) cGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGt < 2:1043764/150‑1 (MQ=255) t‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGAGAATCATCACATGTTTCGCGTGACCATgg > 2:825753/1‑150 (MQ=255) gTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTa < 1:4564583/150‑1 (MQ=255) gTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTa < 2:1004631/150‑1 (MQ=255) gTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTa < 2:184113/150‑1 (MQ=255) tAGATACTGACGGCAGAACGAGACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAg < 2:4747877/150‑1 (MQ=255) aGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGc > 1:1347438/1‑150 (MQ=255) aTACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCgg > 1:1756432/1‑150 (MQ=255) aTACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCgg < 2:1138850/150‑1 (MQ=255) tACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGa > 1:4199554/1‑150 (MQ=255) aCTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGaa > 1:3951476/1‑150 (MQ=255) aCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATg > 1:2148982/1‑150 (MQ=255) aGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATCATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtca > 2:4115439/1‑150 (MQ=255) aGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtca > 2:1366420/1‑150 (MQ=255) gAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtcat > 2:1630982/1‑150 (MQ=255) aCGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtcatca < 2:4229811/150‑1 (MQ=255) aTACGACACCCGCATCGATGTCCACCATCGTGATTGACTCAACGTTTTTCTGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTGTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCAtct < 1:556848/150‑1 (MQ=255) tACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCAtctc < 1:3467112/150‑1 (MQ=255) aCGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCa < 2:921177/150‑1 (MQ=255) cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATTATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt < 2:1460696/150‑1 (MQ=255) cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt < 2:450197/150‑1 (MQ=255) cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt > 1:4165439/1‑150 (MQ=255) cGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat < 1:3647507/150‑1 (MQ=255) cGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat > 2:768945/1‑150 (MQ=255) cATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa < 1:847324/150‑1 (MQ=255) cGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACt < 2:3285237/150‑1 (MQ=255) aTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcg < 1:1618760/150‑1 (MQ=255) tCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcgtcg > 2:333771/1‑150 (MQ=255) ccaccaTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgc > 1:2010685/1‑150 (MQ=255) caccaTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgcg < 2:2602300/150‑1 (MQ=255) | AGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCG > CP009273/132483‑132780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |