Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,865,422 C→A 100% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,865,4220CA95.6% 631.4 / 16.3 181R261R (CGA→AGA) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (85/88);  total (89/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 183 total aligned reads displayed.

ACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTT  >  CP009273/1865276‑1865568
                                                                                                                                                  |                                                                                                                                                  
 caATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGAtt                                                                                                                                                >  2:501831/1‑150 (MQ=255)
  aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                               <  2:440446/150‑1 (MQ=255)
   aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAg                                                                                                                                              >  2:3287873/1‑150 (MQ=255)
   aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGAGCACAAGATTat                                                                                                                                              >  2:3288249/1‑149 (MQ=255)
    tCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGc                                                                                                                                             <  1:1624054/150‑1 (MQ=255)
      tGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCa                                                                                                                                           >  2:1146866/1‑150 (MQ=255)
       gAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAg                                                                                                                                          >  2:2650993/1‑150 (MQ=255)
          cAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATAATTCGCGCAAGAATAGCCAGCGa                                                                                                                                       >  2:3338190/1‑150 (MQ=255)
             ttATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACt                                                                                                                                    <  2:3543110/150‑1 (MQ=255)
               ataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTAt                                                                                                                                  <  1:3579944/150‑1 (MQ=255)
                taACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATg                                                                                                                                 >  1:554728/1‑150 (MQ=255)
                 aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                                >  1:752450/1‑150 (MQ=255)
                  aCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATTCGCACAATATTAGCCCGCGAACTATGGc                                                                                                                               >  1:2559917/1‑150 (MQ=255)
                    gCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCg                                                                                                                             >  2:137873/1‑150 (MQ=255)
                    gCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCg                                                                                                                             >  2:2003910/1‑150 (MQ=255)
                       gACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                          <  1:930609/150‑1 (MQ=255)
                        tcTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTaa                                                                                                                         <  1:2052802/149‑1 (MQ=255)
                        aCTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTaa                                                                                                                         <  2:3504827/150‑1 (MQ=255)
                         cTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAAt                                                                                                                        >  1:177624/1‑150 (MQ=255)
                          tATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATa                                                                                                                       <  1:4431846/150‑1 (MQ=255)
                           aTTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAg                                                                                                                      >  2:1579999/1‑150 (MQ=255)
                             tCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCa                                                                                                                    <  1:1583320/150‑1 (MQ=255)
                             tCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCa                                                                                                                    >  2:2223274/1‑150 (MQ=255)
                              cTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCat                                                                                                                   <  2:838955/150‑1 (MQ=255)
                                ttGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCatta                                                                                                                 <  1:4385787/150‑1 (MQ=255)
                                ttGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCatta                                                                                                                 <  2:2245374/150‑1 (MQ=255)
                                ttGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCatta                                                                                                                 <  2:1416127/150‑1 (MQ=255)
                                    aTGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATtattat                                                                                                             >  1:4289236/1‑150 (MQ=255)
                                    aTGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATtattat                                                                                                             <  2:3998883/150‑1 (MQ=255)
                                     tGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATg                                                                                                            >  1:4723692/1‑150 (MQ=255)
                                       tcgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTc                                                                                                          >  2:1957898/1‑150 (MQ=255)
                                       tcgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTc                                                                                                          >  1:1308656/1‑150 (MQ=255)
                                         gtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                        <  2:3699933/150‑1 (MQ=255)
                                          tcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTgg                                                                                                       >  2:133169/1‑150 (MQ=255)
                                           cgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGc                                                                                                      >  2:3305560/1‑150 (MQ=255)
                                             aTTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCAt                                                                                                    <  2:575720/150‑1 (MQ=255)
                                                aCTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGc                                                                                                 >  2:76814/1‑150 (MQ=255)
                                                 cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCt                                                                                                >  1:4282225/1‑150 (MQ=255)
                                                 cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCt                                                                                                >  1:858217/1‑150 (MQ=255)
                                                         aCAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCa                                                                                        <  2:4472255/150‑1 (MQ=255)
                                                         aCAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCa                                                                                        <  2:4472262/150‑1 (MQ=255)
                                                           aaCCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAac                                                                                      <  1:1202521/150‑1 (MQ=255)
                                                               aTACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttac                                                                                  <  2:1762439/150‑1 (MQ=255)
                                                                tACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACAGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACAATGGCAGTTAATAGCATTATTATTTCTGTCATATCTATGCTGCAacttact                                                                                 >  2:960347/1‑150 (MQ=255)
                                                                 aCTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttactt                                                                                <  1:3107210/150‑1 (MQ=255)
                                                                  cTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTc                                                                               >  1:2153202/1‑150 (MQ=255)
                                                                  cTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTc                                                                               >  2:2691538/1‑150 (MQ=255)
                                                                     cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                            <  1:1241812/150‑1 (MQ=255)
                                                                     cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                            <  2:717166/150‑1 (MQ=255)
                                                                        ccTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGa                                                                         <  1:3035738/150‑1 (MQ=255)
                                                                        ccTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGa                                                                         >  2:1574797/1‑150 (MQ=255)
                                                                         cTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGAc                                                                        >  1:2231206/1‑150 (MQ=255)
                                                                           ggggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGActc                                                                      <  1:2767056/150‑1 (MQ=255)
                                                                            gggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGActct                                                                     <  1:4750021/150‑1 (MQ=255)
                                                                             ggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTg                                                                    >  2:4705291/1‑150 (MQ=255)
                                                                                  cAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGa                                                               >  2:720100/1‑150 (MQ=255)
                                                                                     cTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGta                                                            >  2:1942473/1‑150 (MQ=255)
                                                                                      tataCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtat                                                           <  1:3368110/150‑1 (MQ=255)
                                                                                       ataCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtata                                                          <  1:2498061/150‑1 (MQ=255)
                                                                                           aaaaaTATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATta                                                      <  2:4125229/150‑1 (MQ=255)
                                                                                                tatTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTa                                                 >  1:3793757/1‑150 (MQ=255)
                                                                                                 atTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTAt                                                >  1:1381826/1‑150 (MQ=255)
                                                                                                    ggTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTg                                             >  2:4580411/1‑150 (MQ=255)
                                                                                                    ggTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTg                                             >  1:3916986/1‑150 (MQ=255)
                                                                                                       ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                          <  1:905675/150‑1 (MQ=255)
                                                                                                       ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                          >  1:135139/1‑150 (MQ=255)
                                                                                                       ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                          <  2:2858134/150‑1 (MQ=255)
                                                                                                         gTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtata                                        <  1:4093607/150‑1 (MQ=255)
                                                                                                          tCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTatat                                       <  1:4686900/150‑1 (MQ=255)
                                                                                                            tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATTTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                     >  1:837362/1‑150 (MQ=255)
                                                                                                            tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                     >  1:341986/1‑150 (MQ=255)
                                                                                                              tgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGt                                   <  1:3458786/150‑1 (MQ=255)
                                                                                                              tgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGt                                   <  2:1373977/150‑1 (MQ=255)
                                                                                                              tgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGt                                   <  2:3765119/150‑1 (MQ=255)
                                                                                                               gggCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTc                                  >  2:1650591/1‑150 (MQ=255)
                                                                                                                   aTTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCgg                              >  1:343700/1‑150 (MQ=255)
                                                                                                                   aTTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCgg                              >  1:4627292/1‑150 (MQ=255)
                                                                                                                      tttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                           <  1:3197255/150‑1 (MQ=255)
                                                                                                                      tttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                           <  1:1511586/150‑1 (MQ=255)
                                                                                                                          cTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGaa                       >  2:383034/1‑150 (MQ=255)
                                                                                                                           tGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAg                      <  2:1690590/150‑1 (MQ=255)
                                                                                                                           tGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAg                      <  2:1691137/150‑1 (MQ=255)
                                                                                                                            gTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGt                     >  1:3054440/1‑150 (MQ=255)
                                                                                                                              tCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGttt                   >  2:837556/1‑150 (MQ=255)
                                                                                                                                 ttattaTCATGCGCACAAGATTAGCCAGCGAACTATTGCCGTTAATATCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCa                <  2:4233959/150‑1 (MQ=255)
                                                                                                                                  tattatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAg               >  1:1634704/1‑150 (MQ=255)
                                                                                                                                  tattatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAg               >  2:2952598/1‑150 (MQ=255)
                                                                                                                                   attatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGt              <  1:501831/150‑1 (MQ=255)
                                                                                                                                     tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa            >  1:2041124/1‑150 (MQ=255)
                                                                                                                                     tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa            >  1:3009275/1‑150 (MQ=255)
                                                                                                                                         aTGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtg        >  2:4285660/1‑150 (MQ=255)
                                                                                                                                         aTGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtg        >  1:2953262/1‑150 (MQ=255)
                                                                                                                                         aTGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtg        >  2:3259493/1‑150 (MQ=255)
                                                                                                                                         aTGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtg        >  2:2921033/1‑150 (MQ=255)
                                                                                                                                            cgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATATTTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt     <  1:1969362/150‑1 (MQ=255)
                                                                                                                                            cgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt     >  1:2340623/1‑150 (MQ=255)
                                                                                                                                             gcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttg    >  1:2633723/1‑150 (MQ=255)
                                                                                                                                             gcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttg    >  1:2692637/1‑150 (MQ=255)
                                                                                                                                              cacaAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttgt   >  2:3612976/1‑150 (MQ=255)
                                                                                                                                               acaaGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGTGATTGAAGTTTACAGTAATCtgtttgtt  >  2:245205/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTT  >  CP009273/1865276‑1865568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑