BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,074,680	A→T	60.3%	W22R (TGG→AGG)	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,074,680	0	A	T	60.3%	220.7 / inf	252	W22R (TGG→AGG)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand): ref base A (53/47); new base T (80/72); total (133/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG  >  CP009273/2074533‑2074826

ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCg                                                                                                                                                  <  1:740389/150‑1 (MQ=255)

     acgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCa                                                                                                                                             <  1:1616745/150‑1 (MQ=255)

      cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCaa                                                                                                                                            >  1:4303749/1‑150 (MQ=255)

      cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCaa                                                                                                                                            >  2:3121676/1‑150 (MQ=255)

      cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCaa                                                                                                                                            >  2:752896/1‑150 (MQ=255)

        acgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACg                                                                                                                                          <  2:4038218/150‑1 (MQ=255)

          ggTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACgag                                                                                                                                        >  1:2548797/1‑150 (MQ=255)

          ggTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCAGTTCCCTCGGGCCAACgag                                                                                                                                        <  2:3599738/150‑1 (MQ=255)

              tCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATgg                                                                                                                                    >  2:3992296/1‑150 (MQ=255)

              tCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATgg                                                                                                                                    >  2:620037/1‑150 (MQ=255)

                gCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATGGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGaa                                                                                                                                  <  2:2459489/150‑1 (MQ=255)

                   gCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAcc                                                                                                                               <  2:1651336/150‑1 (MQ=255)

                    cGTAATTTTTCGGCGTGTGTTTCATCTGTATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCt                                                                                                                              <  1:478130/150‑1 (MQ=255)

                    cGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGACACCt                                                                                                                              >  2:2781402/1‑150 (MQ=255)

                       aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                           >  1:3054987/1‑150 (MQ=255)

                       aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                           >  1:2339644/1‑150 (MQ=255)

                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     <  1:2961172/150‑1 (MQ=255)

                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     >  2:18315/1‑150 (MQ=255)

                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     <  1:446058/150‑1 (MQ=255)

                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGACTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     >  2:19266/1‑150 (MQ=255)

                              cggcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTAc                                                                                                                    <  1:3788494/150‑1 (MQ=255)

                                  gggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                                                                                >  1:30789/1‑150 (MQ=255)

                                  gggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                                                                                >  2:1042069/1‑150 (MQ=255)

                                      gtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTctct                                                                                                            <  2:3265561/150‑1 (MQ=255)

                                       tttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCtt                                                                                                           <  1:4228445/150‑1 (MQ=255)

                                           aTCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTg                                                                                                       >  1:3939332/1‑150 (MQ=255)

                                           aTCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTg                                                                                                       <  2:2284819/150‑1 (MQ=255)

                                             cTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCt                                                                                                     >  2:3809675/1‑150 (MQ=255)

                                              tGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCtt                                                                                                    >  2:197113/1‑150 (MQ=255)

                                                   tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCACCGGTACGTTTCTCTTCCTGCTTAAtct                                                                                               >  1:543719/1‑150 (MQ=255)

                                                   tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtct                                                                                               >  1:1135011/1‑150 (MQ=255)

                                                       gTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTa                                                                                           >  1:4393391/1‑150 (MQ=255)

                                                         aaTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                         >  2:2735768/1‑150 (MQ=255)

                                                         aaTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                         <  1:1733364/150‑1 (MQ=255)

                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        >  1:2524893/1‑150 (MQ=255)

                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        <  2:2790076/150‑1 (MQ=255)

                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        >  2:2515672/1‑150 (MQ=255)

                                                             gACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCAt                                                                                     >  1:1224370/1‑150 (MQ=255)

                                                             gACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCAt                                                                                     <  1:1499608/150‑1 (MQ=255)

                                                               cAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGa                                                                                   >  2:610555/1‑150 (MQ=255)

                                                                aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                  >  1:2273123/1‑150 (MQ=255)

                                                                aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                  >  1:1303279/1‑150 (MQ=255)

                                                                  cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGc                                                                                >  1:3143082/1‑150 (MQ=255)

                                                                  cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGc                                                                                >  2:4654272/1‑150 (MQ=255)

                                                                   aaCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGcc                                                                               >  2:4245241/1‑150 (MQ=255)

                                                                      ccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCATCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGTGCCAACGAGATGGAAACCTGAAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                                                                            <  2:1491711/150‑1 (MQ=255)

                                                                       cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                           >  2:4741993/1‑150 (MQ=255)

                                                                       cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                           <  2:4186648/150‑1 (MQ=255)

                                                                        aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                          <  1:2898780/150‑1 (MQ=255)

                                                                        aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                          >  1:3104936/1‑150 (MQ=255)

                                                                        aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                          <  2:1436020/150‑1 (MQ=255)

                                                                            cTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAt                                                                      <  1:4090704/150‑1 (MQ=255)

                                                                               cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACt                                                                   >  2:1336994/1‑150 (MQ=255)

                                                                                    caATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtttt                                                              <  2:4553237/150‑1 (MQ=255)

                                                                                      atatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                            <  2:167598/150‑1 (MQ=255)

                                                                                      atatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                            <  1:353677/150‑1 (MQ=255)

                                                                                        atTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtata                                                          >  1:2463039/1‑150 (MQ=255)

                                                                                          ttttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtatata                                                        <  1:3407952/150‑1 (MQ=255)

                                                                                            ttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACa                                                      <  2:1911042/150‑1 (MQ=255)

                                                                                             tGCTATCTAACCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGTTACGTTTATATTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAg                                                     >  1:789628/1‑150 (MQ=255)

                                                                                              gCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGt                                                    >  2:2907413/1‑150 (MQ=255)

                                                                                               cTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAGTGATACTGTTTTTATATACAGta                                                   >  1:3581381/1‑150 (MQ=255)

                                                                                                 aTCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGtata                                                 >  1:4693183/1‑150 (MQ=255)

                                                                                                 aTCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGtata                                                 >  2:2418646/1‑150 (MQ=255)

                                                                                                   cTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGt                                               >  2:69775/1‑150 (MQ=255)

                                                                                                   cTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGt                                               >  1:2342577/1‑150 (MQ=255)

                                                                                                    tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                              >  2:895161/1‑150 (MQ=255)

                                                                                                     aCCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTg                                             >  2:1247655/1‑150 (MQ=255)

                                                                                                     aCCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTg                                             <  2:4477298/150‑1 (MQ=255)

                                                                                                      cccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGc                                            <  2:3884141/150‑1 (MQ=255)

                                                                                                       ccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCa                                           >  1:1942042/1‑150 (MQ=255)

                                                                                                        cacaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaa                                          <  2:373736/150‑1 (MQ=255)

                                                                                                          cctcatcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaaaa                                        <  1:3773429/148‑1 (MQ=255)

                                                                                                            tcatcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAtt                                      >  1:3600667/1‑150 (MQ=255)

                                                                                                             catcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTa                                     <  1:4654272/150‑1 (MQ=255)

                                                                                                             catcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTa                                     >  2:10224/1‑150 (MQ=255)

                                                                                                              atcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGGATAGTTGCAAAATTaa                                    >  1:1063836/1‑150 (MQ=255)

                                                                                                              atcaACTGCTCAAAGCCTTTCTTTACCGTCTGGTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTaa                                    <  2:3218513/150‑1 (MQ=255)

                                                                                                               tcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTaaa                                   >  1:2048963/1‑150 (MQ=255)

                                                                                                                  aCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAcc                                >  2:215947/1‑150 (MQ=255)

                                                                                                                    tGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcac                              >  2:4715136/1‑150 (MQ=255)

                                                                                                                      cTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACaa                            <  2:3939332/150‑1 (MQ=255)

                                                                                                                        cAAAGCCTTTCTTTACCGTCTTTGCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGATTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAgg                          <  1:88396/150‑1 (MQ=255)

                                                                                                                        cAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAgg                          <  2:2618825/150‑1 (MQ=255)

                                                                                                                        cAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAgg                          >  2:1730300/1‑150 (MQ=255)

                                                                                                                          ccgCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGaa                        >  1:986312/3‑150 (MQ=255)

                                                                                                                           aGCATTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAAt                       <  2:3080463/150‑1 (MQ=255)

                                                                                                                             ccTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACATTATAGTTGCAAAATTAAAACCACAAGGAATGa                     <  1:3699648/150‑1 (MQ=255)

                                                                                                                               tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCACAATTAAAACCACAAGGAATGAgt                   >  1:3568655/1‑150 (MQ=255)

                                                                                                                               tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgt                   <  1:3370247/150‑1 (MQ=255)

                                                                                                                                ttctttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgtg                  <  2:2403307/150‑1 (MQ=255)

                                                                                                                                  ctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGTGTGtt                >  2:3018612/1‑150 (MQ=255)

                                                                                                                                     tACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAt             <  1:1538767/150‑1 (MQ=255)

                                                                                                                                        cGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTAt          >  2:4354503/1‑150 (MQ=255)

                                                                                                                                        cGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTAt          >  1:3924816/1‑150 (MQ=255)

                                                                                                                                         gTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg         <  2:2060380/150‑1 (MQ=255)

                                                                                                                                           cTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCg       <  2:1822442/150‑1 (MQ=255)

                                                                                                                                              ttCCCTCGGTCCAACGAGATGTAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGc    <  2:3347671/150‑1 (MQ=255)

                                                                                                                                                cccTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCCTTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAg  <  1:4051758/150‑1 (MQ=255)

                                                                                                                                                cccTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAg  >  2:2341026/1‑150 (MQ=255)

CCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG  >  CP009273/2074533‑2074826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`