Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,169,117 A→T 7.1% D228E (GAT→GAA)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,169,1170AT 7.1% 956.8 / 46.8 269D228E (GAT→GAA) gatZD‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base A (132/118);  new base T (8/11);  total (140/129)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 273 total aligned reads displayed.

AATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGC  >  CP009273/2168968‑2169266
                                                                                                                                                     |                                                                                                                                                     
aaTATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGa                                                                                                                                                       >  2:2629466/1‑150 (MQ=255)
aaTATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGa                                                                                                                                                       <  1:717334/150‑1 (MQ=255)
 atatTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGAt                                                                                                                                                      >  2:2592293/1‑150 (MQ=255)
  tcttGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATc                                                                                                                                                     <  2:4561229/148‑1 (MQ=255)
  tatTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATc                                                                                                                                                     <  2:462880/150‑1 (MQ=255)
    ttGCATAGTGATCGCGGACTAATTCACTATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCaa                                                                                                                                                   <  1:3502191/150‑1 (MQ=255)
     tGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCaaa                                                                                                                                                  <  2:4547706/150‑1 (MQ=255)
      gCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAAt                                                                                                                                                 <  1:4337852/150‑1 (MQ=255)
        aaaGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTc                                                                                                                                               >  2:306922/1‑150 (MQ=255)
             gATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCacac                                                                                                                                          <  2:830858/150‑1 (MQ=255)
             gATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCacac                                                                                                                                          >  2:2987454/1‑150 (MQ=255)
              aTCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACAcc                                                                                                                                         >  1:856086/1‑150 (MQ=255)
                  cgGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCACACCCGGc                                                                                                                                     >  2:2926801/1‑150 (MQ=255)
                  cgGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGc                                                                                                                                     >  2:3879890/1‑150 (MQ=255)
                    gACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTg                                                                                                                                   >  2:381362/1‑150 (MQ=255)
                       tAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGcac                                                                                                                                >  1:1803431/1‑150 (MQ=255)
                        aaTTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATCATGGATAATATTGCTGTGATCAAATACCACACCCGGCTGcacc                                                                                                                               >  2:1262924/1‑150 (MQ=255)
                            cccAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCACACCCGGCTGCACCACGa                                                                                                                           >  1:2668678/1‑150 (MQ=255)
                              cAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATg                                                                                                                         >  1:4522218/1‑150 (MQ=255)
                               aaTAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATgg                                                                                                                        >  1:1232423/1‑150 (MQ=255)
                                  aaGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCaa                                                                                                                     >  1:3519049/1‑150 (MQ=255)
                                   aGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAAt                                                                                                                    <  2:3164031/150‑1 (MQ=255)
                                       gTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATcaca                                                                                                                >  2:1865776/1‑150 (MQ=255)
                                        tCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCacac                                                                                                               <  1:1914553/150‑1 (MQ=255)
                                           gggTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACgtg                                                                                                            <  1:4446769/150‑1 (MQ=255)
                                           gggTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACgtg                                                                                                            >  2:3527094/1‑150 (MQ=255)
                                           gggTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACgtg                                                                                                            <  2:2335527/150‑1 (MQ=255)
                                             gTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGtt                                                                                                          >  2:3935704/1‑150 (MQ=255)
                                             gTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGtt                                                                                                          <  1:3863653/150‑1 (MQ=255)
                                              tCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTa                                                                                                         >  1:535174/1‑150 (MQ=255)
                                                  gTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGc                                                                                                     <  1:3677684/150‑1 (MQ=255)
                                                      tCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCctct                                                                                                 >  2:1970715/1‑150 (MQ=255)
                                                          tAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCa                                                                                             <  2:2551140/150‑1 (MQ=255)
                                                          tAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCa                                                                                             <  2:1380330/150‑1 (MQ=255)
                                                          tAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCa                                                                                             <  1:2715187/150‑1 (MQ=255)
                                                          tAGAATGTGATTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCa                                                                                             <  1:4612682/150‑1 (MQ=255)
                                                             aaTGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGcc                                                                                          <  1:4232568/150‑1 (MQ=255)
                                                             aaTGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGcc                                                                                          >  1:3345503/1‑150 (MQ=255)
                                                             aaTGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGcc                                                                                          >  1:564319/1‑150 (MQ=255)
                                                               tgtgCTTCATAAACCATTCGGGTGTTTTCTTTCCTTTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCa                                                                                        <  1:2146920/150‑1 (MQ=255)
                                                                gtgCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCAc                                                                                       <  2:701184/150‑1 (MQ=255)
                                                                 tgCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACg                                                                                      >  1:4077065/1‑150 (MQ=255)
                                                                 tgCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACg                                                                                      >  1:2001826/1‑150 (MQ=255)
                                                                 tgCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACg                                                                                      >  2:1322790/1‑150 (MQ=255)
                                                                       aTAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAAt                                                                                >  2:2837565/1‑150 (MQ=255)
                                                                          aaCCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATaaa                                                                             >  2:2349242/1‑150 (MQ=255)
                                                                            ccATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAgg                                                                           >  2:4299170/1‑150 (MQ=255)
                                                                              aTTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATAAAATTCCACACCCGGCTGCACCACGATGGAAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGcc                                                                         <  1:4061021/150‑1 (MQ=255)
                                                                               ttCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTTATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCt                                                                        <  1:3211217/150‑1 (MQ=255)
                                                                                tCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCtt                                                                       <  2:1526325/150‑1 (MQ=255)
                                                                                tCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCtt                                                                       <  2:3705413/150‑1 (MQ=255)
                                                                                 cGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCttt                                                                      >  2:3891228/1‑150 (MQ=255)
                                                                                  gggTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCtttt                                                                     >  1:1204812/1‑150 (MQ=255)
                                                                                   ggTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCATATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTg                                                                    <  2:4572711/150‑1 (MQ=255)
                                                                                    gtgtTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGa                                                                   >  2:238769/1‑150 (MQ=255)
                                                                                     tgtTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGAt                                                                  >  2:770420/1‑150 (MQ=255)
                                                                                     tgtTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGAt                                                                  >  1:2353692/1‑150 (MQ=255)
                                                                                             aTCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGcgtacgt                                                          <  1:3320995/150‑1 (MQ=255)
                                                                                                  ttGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGt                                                     <  1:2076017/150‑1 (MQ=255)
                                                                                                    gcgcCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTAt                                                   <  2:634121/150‑1 (MQ=255)
                                                                                                     cgcCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGTTCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTAtt                                                  <  2:2469507/150‑1 (MQ=255)
                                                                                                     cgcCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTAtt                                                  <  1:1042409/150‑1 (MQ=255)
                                                                                                      gcCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTg                                                 >  2:2103739/1‑150 (MQ=255)
                                                                                                         aGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTggcg                                              >  1:1186085/1‑150 (MQ=255)
                                                                                                           cGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTggcggc                                            >  1:950573/1‑150 (MQ=255)
                                                                                                            ggCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCa                                           >  1:2310055/1‑150 (MQ=255)
                                                                                                            ggCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCa                                           >  1:4183086/1‑150 (MQ=255)
                                                                                                            ggCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCACAGTCAGACCACGGGCAAAAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCa                                           >  1:4691917/1‑150 (MQ=255)
                                                                                                               tGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCt                                        <  1:315451/150‑1 (MQ=255)
                                                                                                                gcgcTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCtt                                       >  2:21965/1‑150 (MQ=255)
                                                                                                                gcgcTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCtt                                       <  1:3391262/150‑1 (MQ=255)
                                                                                                                 cgcTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTc                                      <  1:2987454/150‑1 (MQ=255)
                                                                                                                 cgcTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTc                                      >  2:3242967/1‑150 (MQ=255)
                                                                                                                    ttCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAAc                                   >  1:3018299/1‑150 (MQ=255)
                                                                                                                     tCCTGCGGCTTATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACa                                  <  1:4108277/150‑1 (MQ=255)
                                                                                                                       cTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATg                                >  2:1827632/1‑150 (MQ=255)
                                                                                                                             cTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGAt                          <  1:843808/150‑1 (MQ=255)
                                                                                                                              tGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGAtg                         >  1:4322154/1‑150 (MQ=255)
                                                                                                                              tGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGAtg                         >  1:2917557/1‑150 (MQ=255)
                                                                                                                               gATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGAtgt                        >  1:2823783/1‑150 (MQ=255)
                                                                                                                                  aaTGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTa                     >  1:1634001/1‑150 (MQ=255)
                                                                                                                                   aTGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTac                    >  2:1776176/1‑150 (MQ=255)
                                                                                                                                   aTGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTac                    >  1:2528171/1‑150 (MQ=255)
                                                                                                                                      gATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTactga                 >  2:3637402/1‑150 (MQ=255)
                                                                                                                                      gATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTactga                 <  2:4033142/150‑1 (MQ=255)
                                                                                                                                        taataTTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTactgact               >  1:2109755/1‑150 (MQ=255)
                                                                                                                                        taataTTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTactgact               <  1:1703360/150‑1 (MQ=255)
                                                                                                                                         aataTTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTactgactg              <  1:3173850/150‑1 (MQ=255)
                                                                                                                                          atatTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACTTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTActgactga             <  2:4423611/150‑1 (MQ=255)
                                                                                                                                          atatTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTActgactga             <  2:2577556/150‑1 (MQ=255)
                                                                                                                                           tatTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa            <  1:347084/150‑1 (MQ=255)
                                                                                                                                           tatTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGAGGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa            <  2:1664593/150‑1 (MQ=255)
                                                                                                                                           tagtGCTGTGATCAAATTCCACACCCGGCTGCACCACGAGGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa            <  1:2444831/147‑1 (MQ=255)
                                                                                                                                            atTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAt           >  2:1932754/1‑150 (MQ=255)
                                                                                                                                            atTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAt           >  2:4019648/1‑150 (MQ=255)
                                                                                                                                             ttGCTGTGTTCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATg          <  1:2734961/150‑1 (MQ=255)
                                                                                                                                             ttGCTGTGTTCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATg          <  1:1405881/150‑1 (MQ=255)
                                                                                                                                             ttGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATg          <  1:3894225/150‑1 (MQ=255)
                                                                                                                                              tGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATgg         >  1:1004172/1‑150 (MQ=255)
                                                                                                                                                 tgtgATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGgcgc      >  2:3391171/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGTGTTTTCTATCCATTGCGCCAGCGGCTGCGCTTCCTGCGGCTGATAATGGATAATATTGCTGTGATCAAATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGC  >  CP009273/2168968‑2169266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑