BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,237,098	G→T	88.0%	H12N (CAT→AAT)	folE ←	GTP cyclohydrolase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,237,098	0	G	T	88.0%	597.9 / 75.9	218	H12N (CAT→AAT)	folE	GTP cyclohydrolase I
Reads supporting (aligned to +/- strand): ref base G (16/10); new base T (79/113); total (95/123)
Fisher's exact test for biased strand distribution p-value = 5.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 219 total aligned reads displayed.

CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236952‑2237247

cAAACTGTCATCAGCCAGGTCGAGATTCGGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAc                                                                                                                                                     >  2:496069/1‑150 (MQ=255)

cAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAc                                                                                                                                                     <  2:3061732/150‑1 (MQ=255)

 aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                                    <  1:4692880/150‑1 (MQ=255)

 aaaCCGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                                    <  2:27560/150‑1 (MQ=255)

   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:68053/150‑1 (MQ=255)

   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:181861/150‑1 (MQ=255)

   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:181193/150‑1 (MQ=255)

   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:636145/150‑1 (MQ=255)

    cTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAgg                                                                                                                                                 <  1:1429422/150‑1 (MQ=255)

     tGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAggg                                                                                                                                                >  1:2257194/1‑150 (MQ=255)

      gTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGc                                                                                                                                               <  1:738609/150‑1 (MQ=255)

      gTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGc                                                                                                                                               >  1:3871687/1‑150 (MQ=255)

             gCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttct                                                                                                                                        <  2:3366130/150‑1 (MQ=255)

              ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGcttctt                                                                                                                                       >  2:1061373/1‑150 (MQ=255)

                aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                                     <  2:4163192/150‑1 (MQ=255)

                 ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAc                                                                                                                                    >  2:4255066/1‑150 (MQ=255)

                   tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTg                                                                                                                                  <  1:1130719/150‑1 (MQ=255)

                     gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGGTTACagag                                                                                                                                >  1:2837790/1‑150 (MQ=255)

                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                               >  2:1659062/1‑150 (MQ=255)

                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                               <  1:4130204/150‑1 (MQ=255)

                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGt                                                                                                                               >  1:2841984/1‑150 (MQ=255)

                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGTGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                             >  2:4583873/1‑150 (MQ=255)

                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGTGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                             >  2:2201287/1‑150 (MQ=255)

                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGa                                                                                                                             >  1:3679378/1‑150 (MQ=255)

                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCAAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGATTGa                                                                                                                             >  2:1935091/1‑150 (MQ=255)

                          tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATg                                                                                                                           <  2:1207230/150‑1 (MQ=255)

                           cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATgg                                                                                                                          <  1:2365565/150‑1 (MQ=255)

                           cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATgg                                                                                                                          <  1:3518219/150‑1 (MQ=255)

                            agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGTTGGc                                                                                                                         >  1:2540303/1‑150 (MQ=255)

                            agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                         <  1:1000737/150‑1 (MQ=255)

                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                              <  1:3213998/150‑1 (MQ=255)

                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACTTAGTGTTTGCGTTTTTGAttt                                                                                                              >  1:2449808/1‑150 (MQ=255)

                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATTATTGATGGCATTTTTTAttt                                                                                                              >  1:3347859/1‑150 (MQ=255)

                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                              <  2:1793494/150‑1 (MQ=255)

                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttta                                                                                                             >  2:3144063/1‑149 (MQ=255)

                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACTGAGTGATTGCAGTTAtttttta                                                                                                             >  1:1982147/1‑144 (MQ=255)

                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCGTTACTGAGTGATGGCCTTTATGATTtc                                                                                                             >  1:1459945/1‑150 (MQ=255)

                                         tttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtct                                                                                                            <  1:432600/150‑1 (MQ=255)

                                            cGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCt                                                                                                         <  2:1050931/150‑1 (MQ=255)

                                             ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                                                                        <  2:3686667/150‑1 (MQ=255)

                                               tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCa                                                                                                      >  1:1127108/1‑150 (MQ=255)

                                               tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGTGTGATGGGATTTTTTATTTATCCTGCa                                                                                                      >  1:1466872/1‑150 (MQ=255)

                                                cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAg                                                                                                     >  2:245616/1‑150 (MQ=255)

                                                 atatGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTTAAGAGTGATTGCCGTTTTTATTTGTCCAGCAgg                                                                                                    >  1:3177582/1‑150 (MQ=255)

                                                    tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTGAGTGATTGGATTTATTATTTATCCTGCCGgggt                                                                                                 >  2:4027370/1‑150 (MQ=255)

                                                    tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                                                                                 >  2:1143310/1‑150 (MQ=255)

                                                     gACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGtgtg                                                                                                <  2:4502546/150‑1 (MQ=255)

                                                         aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTAATGATTGATGGCATTTATGTTTTATCCTGCAGGTGTGACGc                                                                                            >  1:2358220/1‑150 (MQ=255)

                                                           cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCAAGTATGATTTCTCCGGCAGGTGTGTCGCCt                                                                                          >  2:1394157/1‑150 (MQ=255)

                                                           cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTTCTGAGTGATTGGATTTTTTATTTCTACGGCAGGGGTGTCGccg                                                                                          >  1:3632362/1‑149 (MQ=255)

                                                             aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                        <  2:1236422/150‑1 (MQ=255)

                                                             aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                        <  2:4540828/150‑1 (MQ=255)

                                                              tAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCg                                                                                       <  2:4256941/150‑1 (MQ=255)

                                                                 ggCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGGATTTATTATTTATCCAGCAGGTGTGTCGCCTCCGccc                                                                                    >  1:603388/1‑150 (MQ=255)

                                                                  gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                                   <  1:963425/150‑1 (MQ=255)

                                                                  gCTTTTGCGCGTTTCGTTATCCATGTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                                   <  2:4073174/150‑1 (MQ=255)

                                                                  gCTTGTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                                   <  2:2598236/150‑1 (MQ=255)

                                                                   cTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTTATGGCATTTATGATTTATACAGCAGGTGTGAAGCCTccccccag                                                                                  >  1:4455248/1‑148 (MQ=255)

                                                                   cTTGTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTg                                                                                  <  2:3410532/150‑1 (MQ=255)

                                                                       tGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTg                                                                             >  2:3905265/1‑150 (MQ=255)

                                                                        gcgcGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTgg                                                                            <  2:1148374/150‑1 (MQ=255)

                                                                        gcgcGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTgg                                                                            <  2:782459/150‑1 (MQ=255)

                                                                         cgcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTggg                                                                           <  2:3120560/150‑1 (MQ=255)

                                                                          gcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAAAGATTGATTGGCTGTATGTTTTATACATCAGGGGTGAAGCCTCCGCCCAGC‑GTgggg                                                                          >  1:1029562/1‑150 (MQ=255)

                                                                              ttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGAGCAAATAACGCTTCATTAAACAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCaa                                                                      <  1:1935091/150‑1 (MQ=255)

                                                                                   tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGtta                                                                 >  2:282437/1‑150 (MQ=255)

                                                                                   tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGTTGGCAGTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGC‑GTGGGGGCAAAGtta                                                                 >  1:1883188/1‑150 (MQ=255)

                                                                                    aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGttat                                                                <  2:3520281/150‑1 (MQ=255)

                                                                                    aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGttat                                                                <  2:1639216/150‑1 (MQ=255)

                                                                                     tCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGttatt                                                               <  1:1659062/150‑1 (MQ=255)

                                                                                        aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGGTGGGATTTATGATTTGTGCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTaat                                                            >  2:442610/1‑149 (MQ=255)

                                                                                        aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAg                                                            <  2:232279/150‑1 (MQ=255)

                                                                                         tttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGc                                                           <  2:1708344/150‑1 (MQ=255)

                                                                                               gCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGAttt                                                     <  2:2257194/150‑1 (MQ=255)

                                                                                                cACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTa                                                    <  2:1640078/150‑1 (MQ=255)

                                                                                                 aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTAc                                                   <  1:124955/150‑1 (MQ=255)

                                                                                                 aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTAATTAGTTTTGGGAGTTTTGATTTATCCTTCAGGGGTGTCGCCGCCCCCCAGC‑GGGGGGGGGCAGGTATTAAACAGAGTTAc                                                   >  1:1600206/1‑150 (MQ=255)

                                                                                                    ggcgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGGTTAAAGATTGGAGGCCGTTATTTTTTCTCCTTCAGGGGTGACGCCACCGCCCCGCGGTGGGGCCAAAG‑TAATAAACAGAATTACAGc                                                >  1:4214247/1‑150 (MQ=255)

                                                                                                      cgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCt                                              <  2:945600/150‑1 (MQ=255)

                                                                                                       gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCCAAGTTATTAAGCTGATTTACAGCCTg                                             >  1:3926141/1‑150 (MQ=255)

                                                                                                       gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTg                                             <  2:3268572/150‑1 (MQ=255)

                                                                                                        ggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGa                                            <  1:883780/150‑1 (MQ=255)

                                                                                                           gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatta                                         <  1:496069/150‑1 (MQ=255)

                                                                                                           gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatta                                         <  2:3736168/150‑1 (MQ=255)

                                                                                                              gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtg                                      <  2:541572/150‑1 (MQ=255)

                                                                                                              gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACAGAGTGATGGCATTTATGATTTATACAGCAGGTGTGACGCCGCCGCCCAGC‑CGGGGGGCCAAGTTAATAATCAGATTTACAGCCAGattattt                                      >  1:2887184/1‑149 (MQ=255)

                                                                                                                  tgtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGGTGGGATTTATTATTTATCCTTCAGGTGTGTCGCCACCGCCCAGC‑GTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGattatttttcg                                  >  1:1046049/1‑145 (MQ=255)

                                                                                                                   gtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGC‑GTGGGGGCACAGTTATTAAGCTGATTTACAGCCTGATTATTTTGCGt                                 >  2:286664/1‑150 (MQ=255)

                                                                                                                    tttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTg                                >  2:3368632/1‑150 (MQ=255)

                                                                                                                      tCCAGTCCTCGCGCACCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAg                              <  2:2567926/150‑1 (MQ=255)

                                                                                                                         aGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGGGTTGGCATTTATGTTTTGTCCGGCAGGGGTGTCGCCTACCCCCGGC‑GGGGGGGCAAAGTTATTAAGAGGTTTTACAGCCAGATTATTTTTCGTGAggcg                           >  2:1988776/1‑150 (MQ=255)

                                                                                                                         aGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGC‑GTGGGGGCAAAGTTATTAAGCGGATTTAAAGCCTGATTATTTTGAGTGAggcg                           >  2:4374108/1‑150 (MQ=255)

                                                                                                                                   cAACTAACGCTTCATGAACCAGGGCCGCTTGTTTACTGATTGATGGGATTTATGATTTCTCCTGCAGGTGTGACGCCACCGCCCAGC‑GTGGGGGCAAAGTTATTAATCAGATTTAAAGCCAGATTATTTTTGGTGAGGCGGCGCACaaaa                 >  2:820131/1‑150 (MQ=255)

                                                                                                                                   cAACTAACGCTTCATGAACCAGGGCCGCGTGTTTAAAGAGTGGTGGCATTTATGATTTCTCCAGCCGGTGTGTCGCCGCCCCCCTGC‑GGGGGGGCAAACTTAATAAGATGATTTACAGCCTGAATATTTTGCGTGAGGCGGCGCACaaaa                 >  2:3996843/1‑150 (MQ=255)

                                                                                                                                    aaCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGAGTGAGGCGGCGCACATAAt                >  2:3545653/1‑150 (MQ=255)

                                                                                                                                      cTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATcc              <  1:4583744/150‑1 (MQ=255)

                                                                                                                                      cTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATcc              <  2:4703533/150‑1 (MQ=255)

                                                                                                                                         aCGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt           <  1:2512609/150‑1 (MQ=255)

                                                                                                                                          cGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTa          <  2:3650403/150‑1 (MQ=255)

                                                                                                                                                  gAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGc  <  2:1986338/150‑1 (MQ=255)

CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236952‑2237247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`