Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,362,338 T→A 100% L274Q (CTG→CAG)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,362,3380TA95.8% 1234.8 / 31.3 335L274Q (CTG→CAG) arnAfused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (9/5);  new base A (173/148);  total (182/153)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 336 total aligned reads displayed.

CGATCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCGGGGT  >  CP009273/2362192‑2362485
                                                                                                                                                  |                                                                                                                                                   
cGATCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGa                                                                                                                                                  >  1:2614934/1‑150 (MQ=255)
cGATCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGa                                                                                                                                                  >  2:2946515/1‑150 (MQ=255)
    ccGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATc                                                                                                                                              <  1:3206818/150‑1 (MQ=255)
              gTGCCTTCAGCTATGTTGGCGATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGAc                                                                                                                                    <  2:158786/150‑1 (MQ=255)
              gTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGAc                                                                                                                                    >  1:4111285/1‑150 (MQ=255)
              gTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGAc                                                                                                                                    >  2:1776516/1‑150 (MQ=255)
                      aGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggcgggc                                                                                                                            >  2:3956021/1‑150 (MQ=255)
                      aGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggcgggc                                                                                                                            >  1:1498724/1‑150 (MQ=255)
                        cTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGa                                                                                                                          >  2:4020558/1‑150 (MQ=255)
                         tATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGAc                                                                                                                         >  2:2024016/1‑150 (MQ=255)
                          aTGTTGGCAATCAGAAAGTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACg                                                                                                                        <  2:2839524/150‑1 (MQ=255)
                            gTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGc                                                                                                                      >  2:3891389/1‑150 (MQ=255)
                              tGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCAt                                                                                                                    <  2:4670306/150‑1 (MQ=255)
                               ggCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCATAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGAGTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCAtt                                                                                                                   <  1:2517023/150‑1 (MQ=255)
                                gCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTa                                                                                                                  <  1:4232011/150‑1 (MQ=255)
                                 cAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTAc                                                                                                                 >  1:3435115/1‑150 (MQ=255)
                                  aaTCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACt                                                                                                                >  2:1262566/1‑150 (MQ=255)
                                  aaTCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACt                                                                                                                <  2:3198036/150‑1 (MQ=255)
                                      aGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGc                                                                                                            >  1:1797455/1‑150 (MQ=255)
                                        aaaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAg                                                                                                          <  1:4570292/150‑1 (MQ=255)
                                        aaaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAg                                                                                                          <  1:484216/150‑1 (MQ=255)
                                        aaaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAg                                                                                                          <  2:3548505/150‑1 (MQ=255)
                                             cACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGACATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTc                                                                                                     >  2:791306/1‑150 (MQ=255)
                                             cACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTc                                                                                                     <  2:1684159/150‑1 (MQ=255)
                                              aCCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCg                                                                                                    >  2:2162212/1‑150 (MQ=255)
                                                cGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGTGACGGCATTACTATGCAGGGCTCGCa                                                                                                  >  2:906542/1‑150 (MQ=255)
                                                cGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCa                                                                                                  <  1:3592568/150‑1 (MQ=255)
                                                     ggTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAg                                                                                             <  2:2070530/150‑1 (MQ=255)
                                                     ggTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAg                                                                                             >  1:257964/1‑150 (MQ=255)
                                                     ggTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAg                                                                                             >  1:385197/1‑150 (MQ=255)
                                                        cgtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAgcgc                                                                                          >  1:408551/1‑150 (MQ=255)
                                                         gtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCa                                                                                         >  1:659522/1‑150 (MQ=255)
                                                         gtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCa                                                                                         >  2:442928/1‑150 (MQ=255)
                                                                gtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTg                                                                                  >  2:894577/1‑150 (MQ=255)
                                                                 ttCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTgg                                                                                 >  2:338507/1‑150 (MQ=255)
                                                                   cATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                               >  1:3056527/1‑150 (MQ=255)
                                                                   cATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                               <  2:2311763/150‑1 (MQ=255)
                                                                    aTCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGcc                                                                              >  1:973937/1‑150 (MQ=255)
                                                                      ccTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTg                                                                            >  2:3905652/1‑150 (MQ=255)
                                                                      ccTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTg                                                                            >  2:4096033/1‑150 (MQ=255)
                                                                       cTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTgg                                                                           >  1:1124978/1‑150 (MQ=255)
                                                                         cATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTg                                                                         >  1:858428/1‑150 (MQ=255)
                                                                          aTGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGc                                                                        >  2:3517343/1‑150 (MQ=255)
                                                                            gCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCaa                                                                      <  2:1105998/150‑1 (MQ=255)
                                                                             ccAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAg                                                                     <  1:4007730/150‑1 (MQ=255)
                                                                             ccAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAg                                                                     <  2:3928712/150‑1 (MQ=255)
                                                                             ccAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAg                                                                     >  1:1361650/1‑150 (MQ=255)
                                                                             ccAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAg                                                                     <  1:4308836/150‑1 (MQ=255)
                                                                             ccAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATGACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAg                                                                     >  1:2119997/1‑150 (MQ=255)
                                                                                gCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGtt                                                                  <  2:46253/150‑1 (MQ=255)
                                                                                gCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGtt                                                                  <  2:3126482/150‑1 (MQ=255)
                                                                                     gCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGc                                                             >  2:1349765/1‑150 (MQ=255)
                                                                                        caGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTg                                                          >  2:56891/1‑150 (MQ=255)
                                                                                         aGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGa                                                         >  1:2785293/1‑150 (MQ=255)
                                                                                          gCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGaa                                                        <  1:3974192/150‑1 (MQ=255)
                                                                                           ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAAt                                                       <  1:1245369/150‑1 (MQ=255)
                                                                                           ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAAt                                                       <  1:363881/150‑1 (MQ=255)
                                                                                             ggggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATag                                                     >  1:4413944/1‑150 (MQ=255)
                                                                                              gggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTCTGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagc                                                    <  2:2891051/150‑1 (MQ=255)
                                                                                              gggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagc                                                    <  2:2209066/150‑1 (MQ=255)
                                                                                                 aGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagccag                                                 >  2:3813345/1‑150 (MQ=255)
                                                                                                    gTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcct                                              <  2:4699673/150‑1 (MQ=255)
                                                                                                    gTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcct                                              <  1:769135/150‑1 (MQ=255)
                                                                                                     tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                             >  1:2951705/1‑150 (MQ=255)
                                                                                                     tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctg                                             <  2:1881404/150‑1 (MQ=255)
                                                                                                      gATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgc                                            >  1:1540227/1‑150 (MQ=255)
                                                                                                      gATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgc                                            >  1:1194158/1‑150 (MQ=255)
                                                                                                      gATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgc                                            <  2:683627/150‑1 (MQ=255)
                                                                                                      gATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgc                                            >  1:4673542/1‑150 (MQ=255)
                                                                                                       aTTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgcc                                           <  1:348425/150‑1 (MQ=255)
                                                                                                         ttCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGcctgcctg                                         <  2:4201591/150‑1 (MQ=255)
                                                                                                           cTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCa                                       <  2:3139858/150‑1 (MQ=255)
                                                                                                           cTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCa                                       >  1:4470552/1‑150 (MQ=255)
                                                                                                           cTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCa                                       >  1:1286033/1‑150 (MQ=255)
                                                                                                                     aCTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACg                             >  1:3734732/1‑150 (MQ=255)
                                                                                                                         ctgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGt                         <  1:2368211/150‑1 (MQ=255)
                                                                                                                         ctgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGt                         >  1:1641883/1‑150 (MQ=255)
                                                                                                                          tgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTa                        >  1:2253982/1‑150 (MQ=255)
                                                                                                                          tgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTa                        >  2:3736397/1‑150 (MQ=255)
                                                                                                                             ttGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTAccc                     <  1:4373522/150‑1 (MQ=255)
                                                                                                                             ttGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTAccc                     <  2:1844346/150‑1 (MQ=255)
                                                                                                                              tGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCg                    >  1:3412542/1‑150 (MQ=255)
                                                                                                                              tGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCg                    >  1:1027556/1‑150 (MQ=255)
                                                                                                                                ccTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCggg                  <  1:2732558/150‑1 (MQ=255)
                                                                                                                                  tgtgGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTa                >  2:1439050/1‑150 (MQ=255)
                                                                                                                                  tgtgGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTa                >  2:1049224/1‑150 (MQ=255)
                                                                                                                                  tgtgGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTa                >  2:1717013/1‑150 (MQ=255)
                                                                                                                                   gtgGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTAc               >  2:318498/1‑150 (MQ=255)
                                                                                                                                   gtgGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTAc               <  1:1935482/150‑1 (MQ=255)
                                                                                                                                        gATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATc          >  2:4336176/1‑150 (MQ=255)
                                                                                                                                        gATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATc          >  2:2589167/1‑150 (MQ=255)
                                                                                                                                          tGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCt        >  1:1554940/1‑150 (MQ=255)
                                                                                                                                          tGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCt        <  1:240703/150‑1 (MQ=255)
                                                                                                                                          tGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCt        >  1:3538395/1‑150 (MQ=255)
                                                                                                                                          tGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCt        <  2:586696/150‑1 (MQ=255)
                                                                                                                                           ggCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTc       <  1:2715102/150‑1 (MQ=255)
                                                                                                                                           ggCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTc       >  1:3727979/1‑150 (MQ=255)
                                                                                                                                           ggCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGCTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTc       <  2:853158/150‑1 (MQ=255)
                                                                                                                                               cgcAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCgggg   <  2:721780/150‑1 (MQ=255)
                                                                                                                                               cgcAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCgggg   <  1:2024016/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGATCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCGGGGT  >  CP009273/2362192‑2362485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑