BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,649,384	C→A	7.7%	D112Y (GAC→TAC)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,649,384	0	C	A	7.7%	1028.0 / 45.4	273	D112Y (GAC→TAC)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base C (137/115); new base A (12/9); total (149/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 275 total aligned reads displayed.

GTGCAGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACC  >  CP009273/2649235‑2649531

           tataACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTGCTGCCGGTGCGTTGATGGTGTCACGCACCCAGt                                                                                                                                          >  2:1016587/1‑150 (MQ=255)

            ataACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTAACGCACCCAGTc                                                                                                                                         <  2:2946575/150‑1 (MQ=255)

                ccTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCgatg                                                                                                                                     <  1:4469559/150‑1 (MQ=255)

                ccTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCgatg                                                                                                                                     <  2:4086192/150‑1 (MQ=255)

                ccTTGCTCACGCAGATCGTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCgatg                                                                                                                                     <  1:2076536/150‑1 (MQ=255)

                 cTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCgatga                                                                                                                                    >  1:606934/1‑150 (MQ=255)

                   tGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGatc                                                                                                                                  <  1:1066680/150‑1 (MQ=255)

                   tGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGatc                                                                                                                                  <  1:4186738/150‑1 (MQ=255)

                   tGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGatc                                                                                                                                  >  2:3219897/1‑150 (MQ=255)

                    gCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGatca                                                                                                                                 <  1:1639260/150‑1 (MQ=255)

                     cTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGatcat                                                                                                                                >  1:3206616/1‑150 (MQ=255)

                      tCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGatcatc                                                                                                                               >  1:2331535/1‑150 (MQ=255)

                       cACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGAtcatca                                                                                                                              <  2:2108493/150‑1 (MQ=255)

                          gCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGc                                                                                                                           >  1:3488102/1‑150 (MQ=255)

                          gCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGATGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGc                                                                                                                           >  2:2913060/1‑150 (MQ=255)

                              aTCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGtt                                                                                                                       <  2:2073492/150‑1 (MQ=255)

                               tCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTa                                                                                                                      >  1:2438789/1‑150 (MQ=255)

                                cTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTAt                                                                                                                     <  2:3159582/150‑1 (MQ=255)

                                cTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTAt                                                                                                                     >  1:1306918/1‑150 (MQ=255)

                                  tCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATcc                                                                                                                   >  1:3682711/1‑150 (MQ=255)

                                   cGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCa                                                                                                                  <  2:2760072/150‑1 (MQ=255)

                                   cGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTAACGCACCCAGTCGATGATCATCAGGCGGTTATCCa                                                                                                                  <  2:2013851/150‑1 (MQ=255)

                                    gCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAg                                                                                                                 >  1:3891825/1‑150 (MQ=255)

                                        ttGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTcc                                                                                                             <  1:2195064/150‑1 (MQ=255)

                                         tGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCt                                                                                                            >  2:4318422/1‑150 (MQ=255)

                                         tGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCt                                                                                                            >  2:4325952/1‑150 (MQ=255)

                                            tGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGgc                                                                                                         <  1:3731972/150‑1 (MQ=255)

                                            tGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGgc                                                                                                         <  2:4245350/150‑1 (MQ=255)

                                                ccGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTg                                                                                                     <  1:2447992/150‑1 (MQ=255)

                                                    tAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCa                                                                                                 <  1:4692193/150‑1 (MQ=255)

                                                     aaGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCAt                                                                                                >  1:291245/1‑150 (MQ=255)

                                                          acacGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTcc                                                                                           >  1:193460/1‑150 (MQ=255)

                                                            acGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAg                                                                                         >  2:2395285/1‑150 (MQ=255)

                                                            acGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTAACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAg                                                                                         <  2:4122119/150‑1 (MQ=255)

                                                              gATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGAt                                                                                       <  2:3738827/150‑1 (MQ=255)

                                                               aTCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTAACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATc                                                                                      >  1:4668917/1‑150 (MQ=255)

                                                                 cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCg                                                                                    >  1:2973478/1‑150 (MQ=255)

                                                                 cGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCg                                                                                    <  1:3919498/150‑1 (MQ=255)

                                                                  gaccGCGACGTTGCTGATCAGATCAACAGCACTCTGTGCCAGTTGCGATGGACCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCAACAGGCGGTTATCCAGTACCTGGCGCTGGGCATCGTCCAGATCCgg                                                                                   >  2:3377562/3‑150 (MQ=255)

                                                                     cgcgACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCca                                                                                >  2:2019382/1‑150 (MQ=255)

                                                                        gACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCTTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGTCGCAGGGCATCGTCCAGATCCGGCCacac                                                                             >  2:790934/1‑150 (MQ=255)

                                                                        gACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCacac                                                                             >  2:1527358/1‑150 (MQ=255)

                                                                           gTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCAcaccac                                                                          >  1:1541022/1‑150 (MQ=255)

                                                                              gCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACttt                                                                       <  2:4514927/150‑1 (MQ=255)

                                                                               cTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTa                                                                      <  2:2373290/150‑1 (MQ=255)

                                                                               cTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTa                                                                      <  2:908121/150‑1 (MQ=255)

                                                                                tGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAc                                                                     >  1:3174388/1‑150 (MQ=255)

                                                                                tGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTAACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAc                                                                     <  1:2685468/150‑1 (MQ=255)

                                                                                 gatcagatcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTAcg                                                                    <  1:4528960/150‑1 (MQ=255)

                                                                                    cagatcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGt                                                                 >  1:3512578/1‑150 (MQ=255)

                                                                                     agatcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTg                                                                >  2:2604314/1‑150 (MQ=255)

                                                                                        tcaACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCt                                                             <  2:1061155/150‑1 (MQ=255)

                                                                                         caACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCtt                                                            >  2:3456881/1‑150 (MQ=255)

                                                                                         caACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCtt                                                            <  1:2091843/150‑1 (MQ=255)

                                                                                          aaCAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCttt                                                           >  1:4324651/1‑150 (MQ=255)

                                                                                          aaCAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCttt                                                           >  1:3078541/1‑150 (MQ=255)

                                                                                            cAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCCTCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCg                                                         >  2:3702225/1‑150 (MQ=255)

                                                                                             aGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgg                                                        >  2:4285755/1‑150 (MQ=255)

                                                                                               cACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCgggg                                                      >  2:528852/1‑150 (MQ=255)

                                                                                                 cGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCt                                                    >  2:1336077/1‑150 (MQ=255)

                                                                                                 cGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCt                                                    >  2:1919165/1‑150 (MQ=255)

                                                                                                   cTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCttt                                                  <  1:1519085/150‑1 (MQ=255)

                                                                                                     gtgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGt                                                <  2:2144998/150‑1 (MQ=255)

                                                                                                     gtgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGt                                                <  1:866758/150‑1 (MQ=255)

                                                                                                      tgCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTa                                               <  1:1379143/150‑1 (MQ=255)

                                                                                                        ccAGTTGCGATGGTCCCAATTCTTCTGCCTGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAAc                                             <  1:2834424/150‑1 (MQ=255)

                                                                                                        ccAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAAc                                             >  1:2547598/1‑150 (MQ=255)

                                                                                                           gTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCtt                                          >  1:1675627/1‑150 (MQ=255)

                                                                                                            ttGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTg                                         <  2:577063/150‑1 (MQ=255)

                                                                                                               cGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCa                                      <  2:3403270/150‑1 (MQ=255)

                                                                                                               cGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCGTTGTAACCTTGCCa                                      >  1:4360050/1‑150 (MQ=255)

                                                                                                                gATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAg                                     <  2:234879/150‑1 (MQ=255)

                                                                                                                gATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAg                                     >  1:649416/1‑150 (MQ=255)

                                                                                                                  tGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGaa                                   <  1:1991509/150‑1 (MQ=255)

                                                                                                                   ggTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCCGAAt                                  >  2:1763986/1‑150 (MQ=255)

                                                                                                                   ggTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAAt                                  <  2:3851450/150‑1 (MQ=255)

                                                                                                                     tCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGc                                >  1:272700/1‑150 (MQ=255)

                                                                                                                      cccAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGcc                               >  2:2688093/1‑150 (MQ=255)

                                                                                                                           ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagca                          >  2:2444138/1‑150 (MQ=255)

                                                                                                                           ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagca                          <  1:2219892/150‑1 (MQ=255)

                                                                                                                           ttcttcTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATAATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagca                          >  2:4047206/1‑150 (MQ=255)

                                                                                                                            tcttctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagcag                         <  2:1499144/150‑1 (MQ=255)

                                                                                                                            tcttctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagcag                         <  1:242909/150‑1 (MQ=255)

                                                                                                                            tcttctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCcagcag                         <  2:3502036/150‑1 (MQ=255)

                                                                                                                             cttctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCagcagc                        <  2:1259766/150‑1 (MQ=255)

                                                                                                                                ctGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGAt                     <  1:2977645/150‑1 (MQ=255)

                                                                                                                                     ggTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCa                >  2:1699307/1‑150 (MQ=255)

                                                                                                                                       tGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATc              <  1:4025505/150‑1 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCGGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           <  1:4165150/150‑1 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           <  1:2486688/150‑1 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           <  2:3977305/150‑1 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           >  1:1311526/1‑150 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           >  1:3416750/1‑150 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           <  1:112471/150‑1 (MQ=255)

                                                                                                                                          gTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCCTCCAGATCCGGCCACACCCCATTACGCCTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCCCATCCCa           >  1:3194340/1‑150 (MQ=255)

                                                                                                                                          gTTGATGGTGTAACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCa           >  1:1108536/1‑150 (MQ=255)

                                                                                                                                            tGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGc         >  1:181104/1‑150 (MQ=255)

                                                                                                                                             gATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGcc        <  1:3334262/150‑1 (MQ=255)

                                                                                                                                                   gtCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCAcc  >  1:947758/1‑150 (MQ=255)

                                                                                                                                                   gtCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCAcc  <  1:1257100/150‑1 (MQ=255)

GTGCAGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGGTCCCAATTCTTCTGCCGGTGCGTTGATGGTGTCACGCACCCAGTCGATGATCATCAGGCGGTTATCCAGTTCCTGGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACC  >  CP009273/2649235‑2649531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`