BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,702,530	G→T	7.6%	A78A (GCC→GCA)	rseA ←	anti‑sigma factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,702,530	0	G	T	7.6%	1297.2 / 54.5	347	A78A (GCC→GCA)	rseA	anti‑sigma factor
Reads supporting (aligned to +/- strand): ref base G (160/160); new base T (11/16); total (171/176)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

GACGTTTCAGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCA  >  CP009273/2702381‑2702678

  cGTTTCAGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGc                                                                                                                                                    >  1:4169536/1‑150 (MQ=255)

    tttCAGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCtt                                                                                                                                                  <  1:2448270/150‑1 (MQ=255)

    tttCAGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCtt                                                                                                                                                  >  2:1471909/1‑150 (MQ=255)

      tCAGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTcc                                                                                                                                                <  2:451167/150‑1 (MQ=255)

        aGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGTGCTTCCgg                                                                                                                                              >  2:1006056/1‑150 (MQ=255)

          aTTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGa                                                                                                                                            >  1:2340677/1‑150 (MQ=255)

           ttGTCCATAAAAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCAACGCCCAGTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATATTCTGCCATTGATGAGGCGCAGGCTGGGCTTCCGGGAt                                                                                                                                           >  2:4751706/1‑150 (MQ=255)

              tCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCaa                                                                                                                                        >  1:679717/1‑150 (MQ=255)

               ccATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAAt                                                                                                                                       >  1:4359527/1‑150 (MQ=255)

               ccATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAAt                                                                                                                                       <  1:183051/150‑1 (MQ=255)

                 aTTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGt                                                                                                                                     >  1:140617/1‑150 (MQ=255)

                  ttATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTc                                                                                                                                    >  2:1769254/1‑150 (MQ=255)

                     taGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGcc                                                                                                                                 >  1:4739967/1‑150 (MQ=255)

                         gCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGtt                                                                                                                             <  2:2325345/150‑1 (MQ=255)

                           tGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGa                                                                                                                           >  1:2290802/1‑150 (MQ=255)

                           tGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGa                                                                                                                           <  1:1182860/150‑1 (MQ=255)

                           tGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGa                                                                                                                           >  1:3925432/1‑150 (MQ=255)

                            ggACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGAc                                                                                                                          >  1:4080069/1‑150 (MQ=255)

                               cGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTa                                                                                                                       <  1:159937/150‑1 (MQ=255)

                                  cAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTg                                                                                                                    >  1:2333462/1‑150 (MQ=255)

                                   aaCGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTgg                                                                                                                   >  2:2163764/1‑150 (MQ=255)

                                    aCGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGc                                                                                                                  >  1:3134920/1‑150 (MQ=255)

                                    aCGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGc                                                                                                                  >  2:3467688/1‑150 (MQ=255)

                                     cGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGct                                                                                                                 <  2:2436606/150‑1 (MQ=255)

                                       aTAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGctct                                                                                                               >  1:4282938/1‑150 (MQ=255)

                                        tAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCtctt                                                                                                              <  2:3148872/150‑1 (MQ=255)

                                         aaCTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCtcttc                                                                                                             <  2:1026844/150‑1 (MQ=255)

                                         aaCTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCtcttc                                                                                                             <  2:2642154/150‑1 (MQ=255)

                                             gCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCa                                                                                                         <  1:3570605/150‑1 (MQ=255)

                                               aaGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAAt                                                                                                       <  2:746892/150‑1 (MQ=255)

                                                aGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATg                                                                                                      <  2:3214719/150‑1 (MQ=255)

                                                 gCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATgg                                                                                                     <  2:4247430/150‑1 (MQ=255)

                                                  cGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggc                                                                                                    >  1:3238379/1‑150 (MQ=255)

                                                  cGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggc                                                                                                    >  1:4526360/1‑150 (MQ=255)

                                                   gATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                                   >  2:326817/1‑150 (MQ=255)

                                                   gATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                                   >  1:3752244/1‑150 (MQ=255)

                                                   gATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                                   >  2:1406820/1‑150 (MQ=255)

                                                   gATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                                   >  1:2809900/1‑150 (MQ=255)

                                                   gATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                                   <  2:3386687/150‑1 (MQ=255)

                                                   gATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                                   <  2:1704409/150‑1 (MQ=255)

                                                     tACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATggcggc                                                                                                 >  2:4486236/1‑150 (MQ=255)

                                                           tGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCAc                                                                                           <  1:1249017/150‑1 (MQ=255)

                                                              ggCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCAcgcg                                                                                        <  1:1261966/150‑1 (MQ=255)

                                                              ggCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCAcgcg                                                                                        >  2:4409000/1‑150 (MQ=255)

                                                                cTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTg                                                                                      <  1:172916/150‑1 (MQ=255)

                                                                 tACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGa                                                                                     <  2:3916194/150‑1 (MQ=255)

                                                                   cGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAag                                                                                   <  2:2948934/150‑1 (MQ=255)

                                                                   cGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAag                                                                                   <  1:3884768/150‑1 (MQ=255)

                                                                       cATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGat                                                                               >  1:2912160/1‑150 (MQ=255)

                                                                        aTTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGata                                                                              >  1:3030328/1‑150 (MQ=255)

                                                                        aTTTGGGTAAGCTGTGCCGCCCAAGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGata                                                                              >  2:330584/1‑150 (MQ=255)

                                                                             ggTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGaa                                                                         >  2:872351/1‑150 (MQ=255)

                                                                               tAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAAt                                                                       <  2:4313546/150‑1 (MQ=255)

                                                                                taGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCTGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATg                                                                      <  1:3275448/149‑1 (MQ=255)

                                                                                aaGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATg                                                                      >  2:1336969/1‑150 (MQ=255)

                                                                                    tgtgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGc                                                                  >  1:1937162/1‑150 (MQ=255)

                                                                                      tgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCAc                                                                <  1:648669/150‑1 (MQ=255)

                                                                                      tgCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCAc                                                                >  1:3335403/1‑150 (MQ=255)

                                                                                             cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGa                                                         <  2:4170358/150‑1 (MQ=255)

                                                                                              aCGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAg                                                        >  1:2547359/1‑150 (MQ=255)

                                                                                                ggACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTa                                                      >  2:613452/1‑150 (MQ=255)

                                                                                                 gACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTAt                                                     >  1:2590737/1‑150 (MQ=255)

                                                                                                      aCTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCAccc                                                >  1:855734/1‑150 (MQ=255)

                                                                                                        tttCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCg                                              <  1:2731588/150‑1 (MQ=255)

                                                                                                            tGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCAtt                                          <  2:1474900/150‑1 (MQ=255)

                                                                                                               cAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAg                                       <  2:1112913/150‑1 (MQ=255)

                                                                                                               cAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAg                                       >  2:3628613/1‑150 (MQ=255)

                                                                                                                  aaTGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCa                                    >  1:3952351/1‑150 (MQ=255)

                                                                                                                  aaTGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCa                                    <  2:384594/150‑1 (MQ=255)

                                                                                                                   aTGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCAc                                   >  1:1733971/1‑150 (MQ=255)

                                                                                                                   aTGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCAc                                   >  2:2860690/1‑150 (MQ=255)

                                                                                                                   aTGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCAc                                   <  1:4688117/150‑1 (MQ=255)

                                                                                                                     ggCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACgg                                 <  1:218194/150‑1 (MQ=255)

                                                                                                                         ttttCTGCCATTGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTa                             <  2:2657201/150‑1 (MQ=255)

                                                                                                                         ttttCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTa                             >  2:4037679/1‑150 (MQ=255)

                                                                                                                         ttttCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTa                             <  1:1170837/150‑1 (MQ=255)

                                                                                                                           ttCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAg                           <  2:460758/150‑1 (MQ=255)

                                                                                                                             cTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTg                         <  2:1873607/150‑1 (MQ=255)

                                                                                                                               gCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGAt                       <  1:3380771/150‑1 (MQ=255)

                                                                                                                               gCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGAt                       >  2:1996372/1‑150 (MQ=255)

                                                                                                                                ccATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATa                      >  2:293717/1‑150 (MQ=255)

                                                                                                                                  aTTGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGc                    <  1:1006056/150‑1 (MQ=255)

                                                                                                                                  aTTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGc                    >  2:1225841/1‑150 (MQ=255)

                                                                                                                                  aTTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGc                    <  2:603923/150‑1 (MQ=255)

                                                                                                                                    tGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCTGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCtt                  <  1:3465682/150‑1 (MQ=255)

                                                                                                                                    tGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCtt                  >  1:1087297/1‑150 (MQ=255)

                                                                                                                                    tGATGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCtt                  <  1:3465616/150‑1 (MQ=255)

                                                                                                                                    tGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCtt                  <  2:524382/150‑1 (MQ=255)

                                                                                                                                      aTGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTc                <  2:2611009/150‑1 (MQ=255)

                                                                                                                                       tGCGGCGCAGGCTGTGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTcc               >  2:2979364/1‑150 (MQ=255)

                                                                                                                                       tGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTcc               <  2:3752244/150‑1 (MQ=255)

                                                                                                                                       tGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTcc               <  2:2809900/150‑1 (MQ=255)

                                                                                                                                         cggcgCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCa             <  1:38624/150‑1 (MQ=255)

                                                                                                                                          ggcgCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCCTCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAg            >  1:51254/1‑150 (MQ=255)

                                                                                                                                          ggcgCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAg            <  1:213161/150‑1 (MQ=255)

                                                                                                                                             gcAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGtt         <  1:4714181/150‑1 (MQ=255)

                                                                                                                                             gcAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGtt         <  2:4080304/150‑1 (MQ=255)

                                                                                                                                               aGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGtttt       >  2:4340468/1‑150 (MQ=255)

                                                                                                                                                  cTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTg    >  2:2077419/1‑150 (MQ=255)

                                                                                                                                                   tGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGc   >  1:2430295/1‑150 (MQ=255)

GACGTTTCAGATTGTCCATTATAGTGCTGGACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTCCCAGGTTTTCTGCA  >  CP009273/2702381‑2702678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`