BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,744,009	T→A	5.2%	D20V (GAT→GTT)	grpE ←	heat shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,744,009	0	T	A	5.2%	769.4 / 22.3	212	D20V (GAT→GTT)	grpE	heat shock protein
Reads supporting (aligned to +/- strand): ref base T (79/122); new base A (7/4); total (86/126)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 214 total aligned reads displayed.

CATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGT  >  CP009273/2743861‑2744158

cATTTCGGCTTTTACACGAAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATc                                                                                                                                                      <  2:4702575/150‑1 (MQ=255)

    tCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATg                                                                                                                                                  <  2:899360/150‑1 (MQ=255)

       gCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATa                                                                                                                                               >  1:195478/1‑150 (MQ=255)

        cTTTTACCCGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATaa                                                                                                                                              <  2:3359726/150‑1 (MQ=255)

        cTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATaa                                                                                                                                              >  2:3222/1‑150 (MQ=255)

         ttttACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAAt                                                                                                                                             <  2:1501522/150‑1 (MQ=255)

           ttACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAAttt                                                                                                                                           <  2:1352072/150‑1 (MQ=255)

                 gCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCg                                                                                                                                     <  1:2610377/150‑1 (MQ=255)

                 gCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCg                                                                                                                                     >  1:2289254/1‑150 (MQ=255)

                 gCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCg                                                                                                                                     >  1:3504960/1‑150 (MQ=255)

                   aaaaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCggg                                                                                                                                   <  2:1292716/150‑1 (MQ=255)

                   aaaaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCggg                                                                                                                                   <  2:3954802/150‑1 (MQ=255)

                   aaaaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCggg                                                                                                                                   >  1:355176/1‑150 (MQ=255)

                   aaaaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCggg                                                                                                                                   <  2:258904/150‑1 (MQ=255)

                   aaaaTGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCggg                                                                                                                                   >  2:2337310/1‑150 (MQ=255)

                       tGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCtt                                                                                                                               >  1:1820905/1‑150 (MQ=255)

                       tGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCtt                                                                                                                               <  1:278995/150‑1 (MQ=255)

                        gCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTg                                                                                                                              >  1:1967561/1‑150 (MQ=255)

                             cACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCt                                                                                                                         <  1:1417447/150‑1 (MQ=255)

                             cACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCt                                                                                                                         <  1:3867045/150‑1 (MQ=255)

                               cGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCa                                                                                                                       >  1:452566/1‑150 (MQ=255)

                                gTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAg                                                                                                                      <  1:803497/150‑1 (MQ=255)

                                 ttCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAgg                                                                                                                     >  2:342195/1‑150 (MQ=255)

                                   cACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCg                                                                                                                   <  2:4522392/150‑1 (MQ=255)

                                   cACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCg                                                                                                                   <  2:3880447/150‑1 (MQ=255)

                                   cACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCg                                                                                                                   >  2:3118033/1‑150 (MQ=255)

                                    aCGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGt                                                                                                                  <  1:407388/150‑1 (MQ=255)

                                    aCGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGt                                                                                                                  <  1:1962156/150‑1 (MQ=255)

                                          cTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTg                                                                                                            <  1:2429421/150‑1 (MQ=255)

                                           tGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGt                                                                                                           >  2:3607442/1‑150 (MQ=255)

                                           tGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGt                                                                                                           >  2:4440734/1‑150 (MQ=255)

                                               cTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCtt                                                                                                       <  1:1125500/150‑1 (MQ=255)

                                               cTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCtt                                                                                                       <  1:3692799/150‑1 (MQ=255)

                                                ttCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCttt                                                                                                      <  2:183253/150‑1 (MQ=255)

                                                ttCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCttt                                                                                                      <  2:183228/150‑1 (MQ=255)

                                                ttCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGGTCttt                                                                                                      >  1:726049/1‑150 (MQ=255)

                                                ttCAGCCAGCTGAGCTTCGAGATTCGCAAATTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGACCCTCAGGCGTGTtcttttcttt                                                                                                      >  1:2697483/1‑150 (MQ=255)

                                                 tCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTta                                                                                                     >  1:2247041/1‑150 (MQ=255)

                                                     ccagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTtactac                                                                                                 <  2:883643/150‑1 (MQ=255)

                                                      cagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTactact                                                                                                >  1:1893347/1‑150 (MQ=255)

                                                      cagcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTactact                                                                                                >  1:4278840/1‑150 (MQ=255)

                                                        gcTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCa                                                                                              <  2:4354200/150‑1 (MQ=255)

                                                             gCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAAt                                                                                         <  1:1609701/150‑1 (MQ=255)

                                                             gCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTCTGTTCTTTACTACTCATGAAt                                                                                         >  2:4618491/1‑150 (MQ=255)

                                                                 cGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTtct                                                                                     >  2:4146836/1‑150 (MQ=255)

                                                                    gaTTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTAAACAAGAATTACTCcg                                                                                  >  2:44774/1‑150 (MQ=255)

                                                                        cGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTATTTACTACTCATGAATTTCTCCGCGtt                                                                              >  2:1219967/1‑150 (MQ=255)

                                                                         gCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGttt                                                                             <  1:3411504/150‑1 (MQ=255)

                                                                         gCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGttt                                                                             <  1:1025246/150‑1 (MQ=255)

                                                                         gCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGttt                                                                             <  2:1646894/150‑1 (MQ=255)

                                                                         gCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACACATGAATTTATACGCGttt                                                                             >  1:4692345/1‑150 (MQ=255)

                                                                             cTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGGTCTTTACTACTCATGAATTTCTCCGCGttttttt                                                                         >  1:4171392/1‑150 (MQ=255)

                                                                                   cATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCAtt                                                                   <  1:909790/150‑1 (MQ=255)

                                                                                   cATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTAGCAtt                                                                   >  2:4236719/1‑150 (MQ=255)

                                                                                     tCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCa                                                                 <  1:2222107/150‑1 (MQ=255)

                                                                                      cgcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAt                                                                <  2:1736354/150‑1 (MQ=255)

                                                                                      cgcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAt                                                                >  2:611669/1‑150 (MQ=255)

                                                                                      cgcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAt                                                                <  2:3491132/150‑1 (MQ=255)

                                                                                       gcgcgGATCCACCTGCACAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtc                                                               <  2:361845/150‑1 (MQ=255)

                                                                                         gcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCAtctc                                                             <  2:2304005/150‑1 (MQ=255)

                                                                                            gATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCt                                                          <  2:1490161/150‑1 (MQ=255)

                                                                                            gATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCt                                                          <  1:616417/150‑1 (MQ=255)

                                                                                                   cTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCg                                                   <  2:3967530/150‑1 (MQ=255)

                                                                                                      cTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTAGCATTCATATAGCTAACTTCGcgt                                                >  1:1782520/1‑148 (MQ=255)

                                                                                                       tCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCtta                                               >  1:1545453/1‑150 (MQ=255)

                                                                                                       tCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCtta                                               <  2:1021497/150‑1 (MQ=255)

                                                                                                       tCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCgta                                               >  1:3936643/1‑150 (MQ=255)

                                                                                                         agcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                             <  2:2379367/150‑1 (MQ=255)

                                                                                                         agcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                             <  1:2127146/150‑1 (MQ=255)

                                                                                                         agcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCGGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                             <  1:1349621/150‑1 (MQ=255)

                                                                                                          gcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTGTGTTCTTTAATACTCATGAATTTCTCCGCGTTTTTTTAGCATTCATCTCGCTAACATCGCgtattt                                            >  1:636622/1‑149 (MQ=255)

                                                                                                          gcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatta                                            <  1:1283408/150‑1 (MQ=255)

                                                                                                           cagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTtattat                                           <  1:4096801/150‑1 (MQ=255)

                                                                                                            agAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGGTTTCTGTTCTTTACTACTCATGAATTTCGCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATg                                          >  1:390943/1‑150 (MQ=255)

                                                                                                              aaGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCATATTATtgg                                        >  2:2269746/1‑150 (MQ=255)

                                                                                                              aaGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTAATCAAGAAATTCTCCGCGTTTTTTTAGCATACATCAAGCTAACATAGCGTATTATtgg                                        >  1:4504754/1‑150 (MQ=255)

                                                                                                                gCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGGTTTTTTCGCATTCATCTCGCTAAATTCGCTTATTATggggg                                      >  2:2409394/1‑149 (MQ=255)

                                                                                                                   tCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCa                                   <  2:2136808/150‑1 (MQ=255)

                                                                                                                   tCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCa                                   <  2:2247041/150‑1 (MQ=255)

                                                                                                                      ggCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGtt                                <  2:4278840/150‑1 (MQ=255)

                                                                                                                      ggCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGtt                                <  2:1893347/150‑1 (MQ=255)

                                                                                                                      ggCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGtt                                <  1:3182344/150‑1 (MQ=255)

                                                                                                                      ggCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTATGTTCTTTACAAAACAAGAATTTCTCCGCGTTTTTTTAGCATTAATATAGCAAACATAGCTTAATATGGGGATCAGtt                                >  1:1071855/1‑150 (MQ=255)

                                                                                                                         tCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCa                             <  2:1900997/150‑1 (MQ=255)

                                                                                                                             cTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTGTGTTGTTTACTACTCATTAATTTGTCCGCGTTTTTTTATCATTAATCAAGATAACATCGCGTATTATGGGGATCAGTTTAAGGGt                         >  1:3268834/1‑150 (MQ=255)

                                                                                                                              tGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGtt                        <  1:859567/150‑1 (MQ=255)

                                                                                                                               gCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGttt                       <  2:301245/150‑1 (MQ=255)

                                                                                                                                ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTc                      <  2:1914123/150‑1 (MQ=255)

                                                                                                                                ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTc                      <  1:4634275/150‑1 (MQ=255)

                                                                                                                                ccTCAATCTCTTCGTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTAGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTc                      >  1:1438848/1‑150 (MQ=255)

                                                                                                                                 cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCa                     <  2:537390/150‑1 (MQ=255)

                                                                                                                                    aaTCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAgg                  <  1:4217276/150‑1 (MQ=255)

                                                                                                                                             gTGCTGAACCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTc         <  2:3632642/150‑1 (MQ=255)

                                                                                                                                              tGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTGTGTTGTTTAAAACTAATGAATTTCTACGCGTTTTTTTAGCATTCAACACGCAAAATTCGCTTATTATGGGGATCAGTTTAAGGGTTTAAAGGGAAGCACTca        >  1:3387695/1‑150 (MQ=255)

                                                                                                                                                cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca      <  2:1599031/150‑1 (MQ=255)

                                                                                                                                                cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca      >  2:4712008/1‑150 (MQ=255)

                                                                                                                                                cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTAATACACATGAATTTATCCGCGTTTTTTTAGCATTCATATCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca      >  2:4006718/1‑150 (MQ=255)

                                                                                                                                                  gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACAtt    <  1:516563/150‑1 (MQ=255)

                                                                                                                                                  gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACACATGAATTTATCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACAtt    >  2:4431856/1‑150 (MQ=255)

                                                                                                                                                    tCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGt  <  2:1936780/150‑1 (MQ=255)

CATTTCGGCTTTTACACGCAAAATGCCGTCACGTTCACGGGTCTGGGCTTCAGCCAGCTGAGCTTCGAGATTCGCAACTTTTTCATCGCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGT  >  CP009273/2743861‑2744158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`