Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,841,044 C→A 60.6% A519A (GCG→GCT)  hycC ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,841,0440CA60.6% 206.2 / inf 366A519A (GCG→GCT) hycChydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (71/73);  new base A (107/114);  total (178/188)
Fisher's exact test for biased strand distribution p-value = 9.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 367 total aligned reads displayed.

CATTTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTA  >  CP009273/2840895‑2841192
                                                                                                                                                     |                                                                                                                                                    
cATTTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATc                                                                                                                                                      <  2:1951308/150‑1 (MQ=255)
     gCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGAATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCAt                                                                                                                                                 >  1:4475706/1‑150 (MQ=255)
       gTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATaa                                                                                                                                               <  1:410960/150‑1 (MQ=255)
       gTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATaa                                                                                                                                               >  1:1421615/1‑150 (MQ=255)
        tAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAat                                                                                                                                              <  2:4015248/150‑1 (MQ=255)
        tAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAat                                                                                                                                              <  1:1115369/150‑1 (MQ=255)
        tAGTGTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAat                                                                                                                                              <  1:265916/150‑1 (MQ=255)
         aGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAatg                                                                                                                                             <  2:1892194/150‑1 (MQ=255)
           tttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgat                                                                                                                                           <  1:1005493/150‑1 (MQ=255)
            ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgatg                                                                                                                                          <  1:1027038/150‑1 (MQ=255)
             tCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAAtgatga                                                                                                                                         <  2:2190590/150‑1 (MQ=255)
               aGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAAt                                                                                                                                       >  1:2739281/1‑150 (MQ=255)
               aGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAAt                                                                                                                                       >  1:4494203/1‑150 (MQ=255)
                gCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATg                                                                                                                                      >  1:1429632/1‑150 (MQ=255)
                 cACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATgg                                                                                                                                     >  1:537002/1‑150 (MQ=255)
                 cACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATgg                                                                                                                                     <  1:790924/150‑1 (MQ=255)
                  aCCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgc                                                                                                                                    <  2:4283632/150‑1 (MQ=255)
                   ccGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgca                                                                                                                                   <  2:312475/150‑1 (MQ=255)
                      gcgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATGGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagca                                                                                                                                <  2:1457648/150‑1 (MQ=255)
                       cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGcagcag                                                                                                                               >  1:4034761/1‑150 (MQ=255)
                          aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                            <  1:1229815/150‑1 (MQ=255)
                                cTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggc                                                                                                                      >  1:2592732/1‑150 (MQ=255)
                                    ttCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATc                                                                                                                  <  2:525589/150‑1 (MQ=255)
                                     tCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATca                                                                                                                 >  1:708264/1‑150 (MQ=255)
                                      cACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                                <  2:4606101/150‑1 (MQ=255)
                                       aCCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcagc                                                                                                               <  1:3214923/150‑1 (MQ=255)
                                          ggCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCagcagc                                                                                                            >  2:807890/1‑150 (MQ=255)
                                          ggCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCagcagc                                                                                                            >  1:2538507/1‑150 (MQ=255)
                                            cATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGGCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCaa                                                                                                          >  1:3668646/1‑150 (MQ=255)
                                             aTGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAAc                                                                                                         <  2:3039957/150‑1 (MQ=255)
                                                gCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTg                                                                                                      >  1:3696129/1‑150 (MQ=255)
                                                 cAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGa                                                                                                     <  2:3243010/150‑1 (MQ=255)
                                                  aaaaCCGTGAGAGGTTATCACCATTGATTTTTCGTGGTCGTAAGCGCACACCCAGGCCGCACCTCGGGAACGAGATGGCAAACTATAGCCTTTGCTAATTGCCATAATGATGAATGGCTGCAGCGGGCAGGCAATCAGCAGCAACGTGat                                                                                                    <  1:2446250/150‑1 (MQ=255)
                                                    aaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatca                                                                                                  >  2:1191907/1‑150 (MQ=255)
                                                    aaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatca                                                                                                  >  2:2379473/1‑150 (MQ=255)
                                                    aaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGatca                                                                                                  <  1:597427/150‑1 (MQ=255)
                                                       cGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCg                                                                                               >  1:2194871/1‑150 (MQ=255)
                                                           aGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTg                                                                                           >  2:3207440/1‑150 (MQ=255)
                                                            gCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTga                                                                                          <  1:4745414/150‑1 (MQ=255)
                                                            gCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTga                                                                                          <  2:1550872/150‑1 (MQ=255)
                                                             cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgag                                                                                         >  1:3339674/1‑150 (MQ=255)
                                                             cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgag                                                                                         >  1:1478504/1‑150 (MQ=255)
                                                               gTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGACTGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGaa                                                                                       <  2:1707362/150‑1 (MQ=255)
                                                                tAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGaaa                                                                                      >  2:2320057/1‑150 (MQ=255)
                                                                  aTCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACg                                                                                    >  1:4302052/1‑150 (MQ=255)
                                                                     aCCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtg                                                                                 >  2:1540564/1‑150 (MQ=255)
                                                                        aTTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGgtggtg                                                                              <  1:1951573/150‑1 (MQ=255)
                                                                         ttGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACgg                                                                                   >  2:904170/1‑144 (MQ=255)
                                                                         ttGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGgtgt                                                                             <  2:1426940/150‑1 (MQ=255)
                                                                          tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCAACGCGGGAACGCGATTGCAAACGATCGCATTTGCAAATAGCCATAATGATGAATGGCAGCAGCGTGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGtt                                                                            <  1:4348340/150‑1 (MQ=255)
                                                                            aTTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAg                                                                          >  2:667818/1‑150 (MQ=255)
                                                                                  cGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCt                                                                    >  2:107219/1‑150 (MQ=255)
                                                                                   gTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTc                                                                   >  2:3107769/1‑150 (MQ=255)
                                                                                    tGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTcc                                                                  >  2:4076556/1‑150 (MQ=255)
                                                                                    tGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTcc                                                                  <  2:3782947/150‑1 (MQ=255)
                                                                                            aCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCag                                                          <  2:680828/150‑1 (MQ=255)
                                                                                            aCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCATTGCAAATAGCCATAATGCTGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCag                                                          <  2:4406227/150‑1 (MQ=255)
                                                                                             ccGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCaga                                                         <  1:3088274/150‑1 (MQ=255)
                                                                                              cGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCagag                                                        >  1:953041/1‑150 (MQ=255)
                                                                                               gCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAgg                                                       <  2:383483/150‑1 (MQ=255)
                                                                                                 acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                     <  2:617130/150‑1 (MQ=255)
                                                                                                 acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                     >  2:1237191/1‑150 (MQ=255)
                                                                                                  cacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAc                                                    <  1:1278263/150‑1 (MQ=255)
                                                                                                     ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagc                                                 >  1:1550111/1‑150 (MQ=255)
                                                                                                     ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagc                                                 >  2:1112225/1‑150 (MQ=255)
                                                                                                     ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGATAcagc                                                 >  2:2587927/1‑150 (MQ=255)
                                                                                                      cAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagca                                                <  2:2885075/150‑1 (MQ=255)
                                                                                                       aGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                               >  1:3500513/1‑150 (MQ=255)
                                                                                                       aGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                               >  1:458886/1‑150 (MQ=255)
                                                                                                       aGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                               >  1:2078531/1‑150 (MQ=255)
                                                                                                        ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagc                                              <  1:4222692/150‑1 (MQ=255)
                                                                                                          ccGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagcag                                            >  2:3903009/1‑150 (MQ=255)
                                                                                                           cGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCaga                                           >  1:2301370/1‑150 (MQ=255)
                                                                                                           cGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCaga                                           >  1:4682756/1‑150 (MQ=255)
                                                                                                            gCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagag                                          >  2:1322972/1‑150 (MQ=255)
                                                                                                              aCCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagagag                                        >  1:998905/1‑150 (MQ=255)
                                                                                                              aCCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagagag                                        >  2:2784355/1‑150 (MQ=255)
                                                                                                               ccGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGc                                       >  1:242784/1‑150 (MQ=255)
                                                                                                                   gggAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                                   >  1:3788351/1‑150 (MQ=255)
                                                                                                                   gggAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                                   <  2:3106885/150‑1 (MQ=255)
                                                                                                                   gggAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                                   >  2:2853088/1‑150 (MQ=255)
                                                                                                                    ggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCgg                                  >  1:2002468/1‑150 (MQ=255)
                                                                                                                          cgATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAg                            <  2:4319713/150‑1 (MQ=255)
                                                                                                                              ggCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCAc                        <  1:158322/150‑1 (MQ=255)
                                                                                                                               gCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACg                       >  2:67213/1‑150 (MQ=255)
                                                                                                                                  aaCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcg                    >  2:4658772/1‑150 (MQ=255)
                                                                                                                                   aCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgcgc                   >  1:116767/1‑150 (MQ=255)
                                                                                                                                    cGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgca                  >  1:1710003/1‑150 (MQ=255)
                                                                                                                                    cGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgca                  >  1:2832200/1‑150 (MQ=255)
                                                                                                                                     gATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag                 >  2:2636963/1‑150 (MQ=255)
                                                                                                                                     gATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag                 >  2:590830/1‑150 (MQ=255)
                                                                                                                                      aTCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagc                >  1:2971363/1‑150 (MQ=255)
                                                                                                                                       tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagca               >  1:970060/1‑150 (MQ=255)
                                                                                                                                         gCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATTGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac             >  2:36614/1‑150 (MQ=255)
                                                                                                                                         gCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac             >  2:35575/1‑150 (MQ=255)
                                                                                                                                           cTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAacac           >  1:3418706/1‑150 (MQ=255)
                                                                                                                                           cTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAacac           >  1:441907/1‑150 (MQ=255)
                                                                                                                                             ttGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCg         <  1:277529/150‑1 (MQ=255)
                                                                                                                                              tGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc        >  1:4245972/1‑150 (MQ=255)
                                                                                                                                                cAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCa      >  2:2664259/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CATTTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTA  >  CP009273/2840895‑2841192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑